Abstract:
:In the interest of classifying cases of male factor infertility, we have paid particular attention to the sugar ligand binding properties of the human sperm surface and the functional capacity of the acrosome for exocytosis--key parameters for assessing sperm fertilizing ability. Zona recognition and binding involve the interactions of sperm surface mannose receptors (lectins) with mannose ligands on the zona pellucida. Sperm surface mannose lectins can be visualized by their ability to bind a synthetic model zona ligand, fluorescein isothiocyanate (FITC)-conjugated mannosylated bovine serum albumin (BSA) (Man-FITC-BSA). We now report that Man-FITC-BSA biologically also mimics the effects of solubilized authentic human zonae, in that binding of Man-FITC-BSA results in a time-dependent receptor aggregation and the induction of acrosome exocytosis in capacitated sperm populations from fertile donors. In our assay, the addition of mM amounts of mannose monosaccharide to Man-FITC-BSA increases the number of polyvalent mannose ligands bound by individual spermatozoa and increases the rate of the acrosome reactions induced by Man-FITC-BSA, thereby increasing specimen processing efficiency. We conclude that exposure of human spermatozoa to polyvalent mannose ligands + D-mannose monosaccharide offers a new, convenient and readily available system to study sperm capacity for induced acrosome loss.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Benoff S,Hurley IR,Mandel FS,Cooper GW,Hershlag Adoi
10.1093/molehr/3.10.827subject
Has Abstractpub_date
1997-10-01 00:00:00pages
827-37issue
10eissn
1360-9947issn
1460-2407journal_volume
3pub_type
杂志文章abstract::Sperm capacitation is a maturation event that takes place in the female reproductive tract and is essential for fertilization. A family of phospholipid-binding proteins present in bovine seminal plasma (BSP proteins) binds the sperm membrane at ejaculation and promotes bovine sperm capacitation. Homologues of these pr...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gal098
更新日期:2007-01-01 00:00:00
abstract::The aetiology of cryptorchidism is for the most part unknown and appears to be multifactorial. Recently, a product of Leydig cells termed Leydig insulin-like hormone (INSL3) has been proposed as a putative trophic hormone of the first part of descent. Absence of Insl3 in male mice results in bilateral cryptorchidism a...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/6.4.298
更新日期:2000-04-01 00:00:00
abstract:STUDY QUESTION:Does dynamin regulate human sperm acrosomal exocytosis? SUMMARY ANSWER:Our studies of dynamin localization and function have implicated this family of mechanoenzymes in the regulation of progesterone-induced acrosomal exocytosis in human spermatozoa. WHAT IS KNOWN ALREADY:Completion of an acrosome reac...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gax044
更新日期:2017-10-01 00:00:00
abstract::Ovaries surgically removed for fertility preservation from a total of 24 women served as a source of human small antral follicles, including the follicular fluid (FF) and the corresponding granulosa cells (GC). The FF was used to evaluate the intrafollicular concentrations of anti-Müllerian hormone (AMH), inhibin-B, e...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaq001
更新日期:2010-09-01 00:00:00
abstract::Before the advent of intracytoplasmic sperm injection (ICSI) semen preparation techniques focused on the need to sustain the fertilizing potential of the spermatozoa particularly by reducing oxidative stress. However, for severely oligozoospermic patients treated by ICSI, sperm preparation protocols are used which aim...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/4.5.439
更新日期:1998-05-01 00:00:00
abstract::Ovarian surface epithelial (OSE) cells participate in the formation of the ovarian cortex and are potential targets of oestrogen action. Oestrogens typically act through nuclear oestrogen receptors (ER) of which there are two known subtypes: ERalpha and ERbeta. In view of the potential importance of oestrogen as a loc...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/4.8.811
更新日期:1998-08-01 00:00:00
abstract::The influence of seminal plasma on the mRNA expression of cytokines in human endometrial epithelial and stromal cells and the cytokine production of spermatozoa were investigated in vitro. Seminal plasma and spermatozoa were collected from healthy volunteers and were screened by enzyme-linked immunosorbent assay for c...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gag095
更新日期:2003-12-01 00:00:00
abstract::Although many candidate genes have been studied in pre-eclampsia (PE), the important class of catecholamine receptors that contribute to sympathetic tone and blood pressure regulation has yet to be investigated. We therefore examined the dopamine D4 receptor (DRD4) gene. We performed a prospective family-based study i...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gal004
更新日期:2006-02-01 00:00:00
abstract::Human mitochondrial DNA (mtDNA) rearrangements, including more than 150 deletions and insertions, accumulate with age and are responsible for certain neuromuscular diseases. Human oocytes, arrested for up to 50 years, may express certain mtDNA rearrangements possibly affecting function. Investigations have previously ...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/5.10.927
更新日期:1999-10-01 00:00:00
abstract::The clinical application of quantitative fluorescent polymerase chain reaction (QF-PCR) for rapid prenatal detection of chromosome aneuploidies has been limited in most studies to the detection of autosomal trisomies. Recently it has been shown that a newly identified highly polymorphic marker, termed X22, which maps ...
journal_title:Molecular human reproduction
pub_type: 临床试验,杂志文章
doi:10.1093/molehr/7.10.1001
更新日期:2001-10-01 00:00:00
abstract::Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common defect in fatty acid oxidation. The disease is inherited in an autosomal recessive fashion (carrier frequency around 1 in 70) and probably affects as many as 1 in 10000 new-borns. Affected children usually present within the two first years of li...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/6.12.1165
更新日期:2000-12-01 00:00:00
abstract::The requisite endpoint of mammalian oocyte maturation, whether in vivo or in vitro, is a metaphase II oocyte which is able to be fertilized and which can eventually support normal embryonic development. Oocytes which have been matured in vivo basically fulfill these criteria. On the other hand, a completely different ...
journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
doi:10.1093/molehr/4.1.41
更新日期:1998-01-01 00:00:00
abstract::It is widely assumed that, after ovulation, the human endometrium undergoes specific changes and becomes receptive to the implantation of embryo during the mid-secretory phase. When implantation does not take place, further changes occur which eventually result in the shedding of human endometrium. The present study w...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/6.7.627
更新日期:2000-07-01 00:00:00
abstract::The major regulators of endometrial function are oestrogen and progesterone, which act through binding their nuclear receptors and by activating transcription of their target genes. Interactions between steroid receptors and transcription proteins, e.g. c-JUN/AP-1, can modulate steroid action at the transcriptional le...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/4.12.1110
更新日期:1998-12-01 00:00:00
abstract:STUDY QUESTION:Do human adult Leydig cells (ALCs) within hyperplastic micronodules display characteristics of foetal LCs (FLCs)? SUMMARY ANSWER:The gene expression profiles of FLCs and all ALC subgroups were clearly different, but there were no significant differences in expressed genes between the normally clustered ...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gax012
更新日期:2017-05-01 00:00:00
abstract::Within the context of an oocyte in vitro maturation (IVM) program for reproductive treatment, oocyte cumulus complexes (COCs) derived from follicles <6 mm in patients with PCOS were matured in vitro. Key transcripts related to meiotic maturation (FSHR, LHCGR, EGFR, PGR) and oocyte competence (AREG, ADAMTS, HAS2, PTGS2...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gas038
更新日期:2013-01-01 00:00:00
abstract::We investigated the expression of methyl transferase G9a and methylated histone H3-K9 in fresh human decidual/endometrial tissue of 12 normal early pregnancies and 15 unexplained recurrent spontaneous abortions (URSA). The samples were obtained through dilatation and curettage and collected as per strict inclusion-exc...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gar038
更新日期:2011-11-01 00:00:00
abstract::Tyrosine nitration is a widely used marker of peroxynitrite (ONOO-) produced from the reaction of nitric oxide (NO.) with superoxide (O2(.-)). Since human spermatozoa are able to produce both NO. and O2(.-) during capacitation in vitro, we investigated whether spontaneous tyrosine nitration of proteins occurs in human...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/7.10.913
更新日期:2001-10-01 00:00:00
abstract::Retrograde flow of menstrual blood cells during menstruation is considered as the dominant theory for the development of endometriosis. Moreover, current evidence suggests that endometrial-derived stem cells are key players in the pathogenesis of endometriosis. In particular, endometrial stromal stem cells have been s...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau044
更新日期:2014-09-01 00:00:00
abstract::The endometrium plays a key role in reproduction, and this function is tightly regulated by endogenous and xenobiotic steroids. Sulphation, catalysed by members of the sulphotransferase (SULT) enzyme family, is a major deactivating mechanism for steroid hormones and we have investigated the expression and regulation i...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/5.11.995
更新日期:1999-11-01 00:00:00
abstract::The use of interphase fluorescent in situ hybridization (FISH) has shown that a large number of human embryos exhibit chromosomal abnormalities in vitro. The most common abnormality is mosaicism which is seen in up to 50% of preimplantation embryos at all stages of development. In this study, comparative genomic hybri...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gam087
更新日期:2008-02-01 00:00:00
abstract::Success rate in human pregnancies is believed to be very low and sex-specific mechanisms may operate in prenatal loss. Assuming a sex-differential in prenatal loss exists, we examined genetic markers in biologically plausible targets in the HLA complex, other immune system-related and iron-regulatory genes in 388 heal...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaq047
更新日期:2010-10-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is relevant for normal pregnancy, and abnormalities in VEGF functions are associated with hypertensive disorders of pregnancy. Because there are few studies on how VEGF genetic polymorphisms affect susceptibility to pre-eclampsia (PE), and no studies on how they affect suscept...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gan076
更新日期:2009-02-01 00:00:00
abstract::Olfactory receptors are G protein-coupled transmembrane receptors. Genes encoding olfactory receptors constitute a large gene family of approximately 1000 total member genes. In mammals, a subset of member genes is specifically expressed in the testis (and not in the olfactory mucosa) and olfactory receptor proteins h...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/7.6.553
更新日期:2001-06-01 00:00:00
abstract:STUDY QUESTION:What is the association between placental formyl peptide receptor 2 (FPR2) and trophoblast and endothelial functions in pregnancies affected by foetal growth restriction (FGR)? SUMMARY ANSWER:Reduced FPR2 placental expression in idiopathic FGR results in significantly altered trophoblast differentiation...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gax067
更新日期:2018-02-01 00:00:00
abstract::Prader-Willi syndrome (PWS) is a genomic disorder mostly caused by deletions of 15q11-q13 region (70%). It has been suggested that the particular genomic architecture of 15q11-q13 region, characterized to be flanked by low copy repeats, could predispose it to Non-Allelic Homologous Recombination (NAHR). However, no st...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaq005
更新日期:2010-05-01 00:00:00
abstract::Successful intracytoplasmic sperm injection (ICSI) is dependent upon the competence of the oocyte to respond to the injection of a spermatozoon in the presence of calcium. This study determined if oocytes that failed to become fertilized (absence of any pronuclei) failed to undergo cytoplasmic activation, as ascertain...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/2.12.959
更新日期:1996-12-01 00:00:00
abstract::Prostaglandins (PGs) are important factors in the physiology of human parturition and the control of uterine contractility. We have characterized the expression of 15 genes from all stages of the PG pathway in human pregnant and non-pregnant (NP) myometrium and in other uterine tissues at delivery, and the results sho...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaq057
更新日期:2011-01-01 00:00:00
abstract::Recurrent pregnancy loss (RPL) is a common and distressing disorder. Chromosomal errors in the embryo are the single most common cause, whereas uterine factors are invariably invoked to explain non-chromosomal miscarriages. These uterine factors are, however, poorly defined. The ability of a conceptus to implant in th...
journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
doi:10.1093/molehr/gaq079
更新日期:2010-12-01 00:00:00
abstract::Interleukin (IL)-33, a newly described member of the IL-1 family, has been reported to facilitate primary tumor progression and metastatic dissemination. However, its biological function on decidual stromal cells (DSCs) remains unclear. In this study, we tested the hypothesis whether IL-33 promotes proliferation and i...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gat094
更新日期:2014-04-01 00:00:00