Abstract:
:Nuclear oligomerization domains (NODs) are cytosolic pattern recognition receptors (PRRs), present in epithelial cells, monocytes and dendritic cells. This study details their expression, regulation and role in human endometrium. Real-time PCR showed that NOD1 mRNA is constitutively expressed in endometrium. NOD2 is up-regulated in the late secretory phase of the menstrual cycle suggesting a role in menstruation. Both proteins are immunolocalized in endometrial epithelium, stroma and endothelium. In first trimester, decidua NODs are present in decidualized stroma. NOD function was examined in endometrial stromal cells (ESCs) and endometrial epithelial cells (EEpCs) in vitro. IkappaBalpha is up-regulated by stimulation of ESC and EEpC with an NOD1 ligand. IkappaBalpha, IL-8 and TNFalpha mRNA expression is increased in EEpC by a NOD2 ligand. NOD2 mRNA expression increases in response to IL-1 treatment while NOD1 transcripts are unaltered. NOD1 mRNA is increased in an in vitro model of decidualization of ESC. In summary, we report expression of NOD1 and NOD2 in human endometrium and show that they are differentially regulated. NOD2 and, to a lesser extent, NOD1 can function to increase expression of innate immune molecules in endometrium. NODs may have a role in innate immune protection in the uterus and NOD2 may regulate inflammation associated with menstruation.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
King AE,Horne AW,Hombach-Klonisch S,Mason JI,Critchley HOdoi
10.1093/molehr/gap020subject
Has Abstractpub_date
2009-05-01 00:00:00pages
311-9issue
5eissn
1360-9947issn
1460-2407pii
gap020journal_volume
15pub_type
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journal_title:Molecular human reproduction
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更新日期:2018-02-01 00:00:00
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更新日期:2000-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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更新日期:2016-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-07-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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更新日期:2002-02-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/2.4.265
更新日期:1996-04-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2001-10-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaaa069
更新日期:2020-12-10 00:00:00
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pub_type: 杂志文章
doi:10.1093/molehr/7.11.1039
更新日期:2001-11-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
doi:10.1093/molehr/gam089
更新日期:2008-01-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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更新日期:2011-07-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
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更新日期:2015-05-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2004-10-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/5.5.391
更新日期:1999-05-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2018-10-01 00:00:00