Abstract:
:Recombinant human ZP3 (rhuZP3) generated by Chinese hamster ovary cells transfected with a plasmid containing human ZP3 cDNA was used to study the acrosome reaction (AR) and intracellular calcium fluxes in capacitated human spermatozoa. Conditioned medium containing rhuZP3 significantly induced the AR (P < or = 0.005) in 59.4 +/- 4.7% of spermatozoa (control = 8.5 +/- 3.1%) and caused complete acrosomal loss in a further 17.2 +/- 3.8% of cells (control = 3.7 +/- 0.7%; mean +/- SEM, n = 5). Sperm motility was not affected and acrosomal exocytosis in response to rhuZP3 was also shown to be time-dependent. Basal concentrations of sperm intracellular calcium were measured (82 +/- 7 nM; mean +/- SEM, n = 9). A transient increase in intracellular calcium (typically up to 400-450 nM) occurred within 1 min of rhuZP3 addition and was followed by sustained lower values of calcium (200-400 nM). These responses were dependent on the amount of rhuZP3. This is the first report of zona protein-induced changes in intracellular calcium levels in human spermatozoa. The results support the premise that ZP3 is an agonist of the human sperm AR and that rhuZP3 generated in a eukaryotic cell is effective in this respect.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Brewis IA,Clayton R,Barratt CL,Hornby DP,Moore HDdoi
10.1093/molehr/2.8.583subject
Has Abstractpub_date
1996-08-01 00:00:00pages
583-9issue
8eissn
1360-9947issn
1460-2407journal_volume
2pub_type
杂志文章abstract::In recent years, advances in imaging probes, cutting-edge microscopy techniques and powerful bioinformatics image analysis have markedly expanded the imaging toolbox available to developmental biologists. Apart from traditional qualitative studies, embryonic development can now be investigated in vivo with improved sp...
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更新日期:1997-05-01 00:00:00
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更新日期:1998-10-01 00:00:00
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更新日期:2003-01-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2001-10-01 00:00:00
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更新日期:2010-10-01 00:00:00
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更新日期:2001-03-01 00:00:00
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更新日期:2009-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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doi:10.1093/molehr/3.9.821
更新日期:1997-09-01 00:00:00
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更新日期:2017-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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更新日期:2006-02-01 00:00:00
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更新日期:2017-07-01 00:00:00
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更新日期:1999-10-01 00:00:00
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更新日期:2007-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00