Identification and localization of G protein subunits in human spermatozoa.

abstract：:Immunocytochemistry was used to localize endothelial (eNOS) and inducible (iNOS) nitric oxide synthase in human uterine tissues collected at various stages of the menstrual cycle, after exposure to exogenous progestagens, and in early pregnancy. Endothelial NOS-like immunoreactivity was detected in all specimens in en...

journal_title：Molecular human reproduction

authors： Telfer JF,Irvine GA,Kohnen G,Campbell S,Cameron IT

更新日期：1997-01-01 00:00:00

abstract：:Endometriosis, the presence of ectopic endometrial tissue outside the uterine cavity, is a common disease affecting women during their reproductive years. The aim of this study was to identify the molecular mechanism of transcriptional regulation of inflammatory cyclooxygenase-2 (COX-2) gene during endometriosis by hy...

journal_title：Molecular human reproduction

authors： Kim KH,Kim HY,Kim HH,Lee KS,Cheong J

更新日期：2011-11-01 00:00:00

abstract：:It is widely assumed that, after ovulation, the human endometrium undergoes specific changes and becomes receptive to the implantation of embryo during the mid-secretory phase. When implantation does not take place, further changes occur which eventually result in the shedding of human endometrium. The present study w...

journal_title：Molecular human reproduction

authors： von Wolff M,Thaler CJ,Strowitzki T,Broome J,Stolz W,Tabibzadeh S

更新日期：2000-07-01 00:00:00

abstract：:Multiple types and subtypes, including splice variants, of cyclic nucleotide phosphodiesterases (PDE) have been shown to be expressed in various tissues and organs. They control the intracellular level of cyclic nucleotides and are involved in hormonal signalling. In human spermatozoa, PDE play an important role in th...

journal_title：Molecular human reproduction

authors： Richter W,Dettmer D,Glander H

更新日期：1999-08-01 00:00:00

abstract：:Deletions of the AZFc interval of the human Y chromosome are found in >5% of male patients with idiopathic infertility and are associated with a severely reduced sperm count. The most common deletion type is large (>1 Mb) and removes members of the Y-borne testis-specific gene families of BPY2, CDY1, DAZ, PRY, RBMY2 a...

journal_title：Molecular human reproduction

authors： Saut N,Terriou P,Navarro A,Lévy N,Mitchell MJ

更新日期：2000-09-01 00:00:00

abstract：:We have investigated the possible roles of oncostatin M (OSM), which is a member of the interleukin-6 family of cytokines, in endometrial and endometriotic stromal cell growth. Endometrial and endometriotic stromal cells were collected from the uterus or ovarian chocolate cysts. We observed the expression of mRNA tran...

journal_title：Molecular human reproduction

authors： Ohata Y,Harada T,Fujii A,Yoshida S,Iwabe T,Terakawa N

更新日期：2001-07-01 00:00:00

abstract：:Oligozoospermia (low sperm count) is a common semen deficiency. However, to date, few genetic defects have been identified to cause this condition. Moreover, even fewer molecular genetic diagnostic tests are available for patients with oligozoospermia in the andrology clinic. Based on animal and gene expression studie...

journal_title：Molecular human reproduction

authors： Yatsenko AN,Georgiadis AP,Murthy LJ,Lamb DJ,Matzuk MM

更新日期：2013-06-01 00:00:00

abstract：:Zinc dynamics are essential for oocyte meiotic maturation, egg activation, and preimplantation embryo development. During fertilisation and egg activation, the egg releases billions of zinc atoms (Zn2+) in an exocytotic event termed the 'zinc spark'. We hypothesised that this zinc transport and exocytosis is dependent...

journal_title：Molecular human reproduction

authors： Lee HC,Edmonds ME,Duncan FE,O'Halloran TV,Woodruff TK

更新日期：2020-04-24 00:00:00

abstract：:Charcot-Marie-Tooth (CMT) disease is the 'common' name for a range of hereditary peripheral neuropathies. CMT1 is the most common form and is transmitted in an autosomal dominant manner. CMT1A maps to chromosome 17p11.2 and is caused, in the majority of cases, by a 1.5 Mb DNA duplication, that includes the peripheral ...

journal_title：Molecular human reproduction

authors： De Vos A,Sermon K,De Rijcke M,Goossens V,Henderix P,Van Ranst N,Platteau P,Lissens W,Devroey P,Van Steirteghem A,Liebaers I

更新日期：2003-07-01 00:00:00

abstract：:Oocyte meiosis is sensitive to endogenous and exogenous perturbations that upset the temporal sequence of biochemical reactions during oocyte maturation (OM) and predispose oocytes to aneuploidy. Nicotine is an alkaloid that has been reported to disrupt the rate of OM, reduce ovulation and fertilization rates, and inc...

journal_title：Molecular human reproduction

authors： Mailhes JB,Young D,Caldito G,London SN

更新日期：2000-03-01 00:00:00

abstract：:In recent years, advances in imaging probes, cutting-edge microscopy techniques and powerful bioinformatics image analysis have markedly expanded the imaging toolbox available to developmental biologists. Apart from traditional qualitative studies, embryonic development can now be investigated in vivo with improved sp...

journal_title：Molecular human reproduction

authors： Welling M,Ponti A,Pantazis P

更新日期：2016-03-01 00:00:00

abstract：STUDY QUESTION:What is the prevalence, reproducibility and biological significance of transcriptomic differences between sister blastomeres of the mouse 2-cell embryo? SUMMARY ANSWER:Sister 2-cell stage blastomeres are distinguishable from each other by mRNA analysis, attesting to the fact that differentiation starts ...

journal_title：Molecular human reproduction

authors： Casser E,Israel S,Schlatt S,Nordhoff V,Boiani M

更新日期：2018-07-01 00:00:00

abstract：:Granzyme B (graB) plays a pivotal role in cytotoxic lymphocyte granule-mediated apoptosis through cleavage of intracellular proteins in target cells. Proteinase inhibitor-9 (PI-9) is a potent inhibitor of graB and is highly expressed in cytotoxic lymphocytes. Here, we show by immunohistochemistry that PI-9 is also abu...

journal_title：Molecular human reproduction

authors： Hirst CE,Buzza MS,Sutton VR,Trapani JA,Loveland KL,Bird PI

更新日期：2001-12-01 00:00:00

abstract：:Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...

journal_title：Molecular human reproduction

authors： Catt SL,Sakkas D,Bizzaro D,Bianchi PG,Maxwell WM,Evans G

更新日期：1997-09-01 00:00:00

abstract：:Follicular fluid proteoglycans play an important role in human oocyte maturation, including the development of a fluid-filled compartment and maintenance of the hypocoagulative state of the follicular fluid. Human xylosyltransferase (EC 2.4.2.26, XT) is the key enzyme in the biosynthesis of glycosaminoglycan chains in...

journal_title：Molecular human reproduction

authors： Götting C,Kuhn J,Tinneberg HR,Brinkmann T,Kleesiek K

更新日期：2002-12-01 00:00:00

abstract：:This study was undertaken to localize ryanodine-sensitive Ca2+ stores in human oocytes and to evaluate their role in the Ca2+ oscillations responsible for oocyte activation at fertilization. The addition of ryanodine provoked a Ca2+ discharge from stores localized throughout the ooplasm with the exception of the corti...

journal_title：Molecular human reproduction

authors： Sousa M,Barros A,Tesarik J

更新日期：1996-04-01 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is a heterogeneous condition with unknown etiology and is considered to be the most common endocrine disorder in women of reproductive age. Two meta-analyses are presented here concerning the association of Plasminogen Activator Inhibitor 1 (PAI-1) 4G/5G polymorphism and the methylene-...

journal_title：Molecular human reproduction

authors： Bagos PG

更新日期：2009-01-01 00:00:00

abstract：:The aim of this study was to investigate patterns of gene expression in placental samples from patients with preeclampsia (PE), persistent bilateral uterine artery notching (without PE), and normal controls. This study included placental tissue from nine women with PE, seven with uncomplicated pregnancies and five wit...

journal_title：Molecular human reproduction

authors： Hansson SR,Chen Y,Brodszki J,Chen M,Hernandez-Andrade E,Inman JM,Kozhich OA,Larsson I,Marsál K,Medstrand P,Xiang CC,Brownstein MJ

更新日期：2006-03-01 00:00:00

abstract：:We recently identified the DPY19L2 gene as the main genetic cause of human globozoospermia. Non-genetically characterized cases of globozoospermia were associated with DNA alterations, suggesting that DPY19L2-dependent globozoospermia may be associated with poor DNA quality. However the origins of such defects have no...

journal_title：Molecular human reproduction

authors： Yassine S,Escoffier J,Martinez G,Coutton C,Karaouzène T,Zouari R,Ravanat JL,Metzler-Guillemain C,Lee HC,Fissore R,Hennebicq S,Ray PF,Arnoult C

更新日期：2015-02-01 00:00:00

abstract：:Success rate in human pregnancies is believed to be very low and sex-specific mechanisms may operate in prenatal loss. Assuming a sex-differential in prenatal loss exists, we examined genetic markers in biologically plausible targets in the HLA complex, other immune system-related and iron-regulatory genes in 388 heal...

journal_title：Molecular human reproduction

authors： Ucisik-Akkaya E,Davis CF,Do TN,Morrison BA,Stemmer SM,Amadio WJ,Dorak MT

更新日期：2010-10-01 00:00:00

abstract：:Testis expressed gene 18 (Tex18) is a small gene with one exon of 240 bp, which is specifically expressed in male germ cells. The gene encodes for a protein of 80 amino acids with unknown domain. To investigate the function of (Tex18) gene, we generated mice with targeted disruption of the (Tex18) gene by homologous r...

journal_title：Molecular human reproduction

authors： Jaroszynski L,Dev A,Li M,Meinhardt A,de Rooij DG,Mueller C,Böhm D,Wolf S,Adham IM,Wulf G,Engel W,Nayernia K

更新日期：2007-03-01 00:00:00

abstract：:Testicular germ cell cancer is aetiologically linked to genital malformations and male infertility and is most probably caused by a disruption of embryonic programming and gonadal development during fetal life. In some cases, germ cell neoplasia is associated with a relative reduction of Y chromosomal material (e.g. 4...

journal_title：Molecular human reproduction

authors： Frydelund-Larsen L,Vogt PH,Leffers H,Schadwinkel A,Daugaard G,Skakkebaek NE,Rajpert-De Meyts E

更新日期：2003-09-01 00:00:00

abstract：:Prader-Willi syndrome (PWS) is a genomic disorder mostly caused by deletions of 15q11-q13 region (70%). It has been suggested that the particular genomic architecture of 15q11-q13 region, characterized to be flanked by low copy repeats, could predispose it to Non-Allelic Homologous Recombination (NAHR). However, no st...

journal_title：Molecular human reproduction

authors： Molina O,Blanco J,Vidal F

更新日期：2010-05-01 00:00:00

abstract：:Observational human data and several lines of animal experimental data indicate that maternal obesity impairs offspring health. Here, we comprehensively tested the model that maternal obesity causes defects in the next three generations of oocytes and embryos. We exposed female F0 mice to a high-fat/high-sugar (HF/HS)...

journal_title：Molecular human reproduction

authors： Andreas E,Reid M,Zhang W,Moley KH

更新日期：2019-11-30 00:00:00

abstract：:The Y chromosome carries several spermatogenesis genes distributed in three regions: AZFa, AZFb and AZFc. Microdeletions in these regions have been seen in 10% of sterile males with azoospermia or oligozoospermia, the most frequent of them being characterized by a complete deletion of AZFc region. A partial AZFc delet...

journal_title：Molecular human reproduction

authors： Carvalho CM,Zuccherato LW,Bastos-Rodrigues L,Santos FR,Pena SD

更新日期：2006-04-01 00:00:00

abstract：:This study assesses the role of progesterone receptor membrane component 1 (PGRMC1) in actions of progesterone (P4) on human myometrium during pregnancy and labour. Myometrial tissues were obtained from non-pregnant patients during hysterectomy or pregnant women undergoing C-section at term and preterm, before and dur...

journal_title：Molecular human reproduction

authors： Wu W,Shi SQ,Huang HJ,Balducci J,Garfield RE

更新日期：2011-04-01 00:00:00

abstract：:In the implantation, trophoblasts penetrate maternal decidua by secreting proteases. It has been reported that cathepsins are highly expressed in the mouse villi, and play an important role in normal embryonal growth and decidualization. In this study, we evaluated cathepsins and their endogenous inhibitors, cystatins...

journal_title：Molecular human reproduction

authors： Nakanishi T,Ozaki Y,Blomgren K,Tateyama H,Sugiura-Ogasawara M,Suzumori K

更新日期：2005-05-01 00:00:00

abstract：STUDY HYPOTHESIS:What factors in mouse oocytes are involved in the ageing-related decline in oocyte quality? STUDY FINDING:The maternal effect gene Mater is involved in ageing-related oocyte quality decline in mice. WHAT IS KNOWN ALREADY:Premature loss of centromere cohesion is a hallmark of ageing-related oocyte qua...

journal_title：Molecular human reproduction

authors： Lu YQ,He XC,Zheng P

更新日期：2016-04-01 00:00:00

abstract：:This study was undertaken to evaluate the effects of gamma-aminobutyric acid (GABA) and GABAergic agonists and antagonists on sperm kinematic parameters and hyperactivation, evaluated by a computer-assisted semen analysis (CASA) system, and intracellular cAMP content in 22 normozoospermic semen samples. Because of the...

journal_title：Molecular human reproduction

authors： Calogero AE,Hall J,Fishel S,Green S,Hunter A,D'Agata R

更新日期：1996-10-01 00:00:00

abstract：:Natural killer (NK) cells are the predominant leukocyte in first trimester decidua and play a role in vascular remodelling through interferon gamma (IFNgamma) secretion. Membrane expression of the killer immunoglobulin-like receptor (KIR) KIR2DL4 on peripheral blood NK (pNK) cells is controlled by the 9A/10A transmemb...

journal_title：Molecular human reproduction

authors： Goodridge JP,Lathbury LJ,John E,Charles AK,Christiansen FT,Witt CS

更新日期：2009-08-01 00:00:00