Abstract:
:The Drosophila melanogaster staufen gene encodes an RNA binding protein (Dm Stau) required for the localization and translational repression of mRNAs within the Drosophila oocyte. In mammals translational repression is important for normal spermatogenesis in males and storage of mRNAs in the oocytes of females. In the present study we identified two mouse cDNA expressed sequence tags (ESTs), encoding proteins with significant homology to Dm Stau and used these firstly to screen a mouse kidney cDNA library and secondly to determine whether staufen mRNAs are expressed in the ovaries and testes of mice and rats. Sequence analysis of the cDNAs revealed that they originated from two different genes. Using Northern blots of RNAs from kidneys, ovaries and testes, both cDNAs hybridized to mRNA species of approximately 3 kb in all three tissues. On sections of mouse ovaries, staufen mRNA was localized specifically to oocytes. On sections of mouse testes, staufen mRNA was expressed in spermatocytes found in seminiferous tubules at stages VI-XII of the spermatogenic cycle. In conclusion, we have shown that the mammalian homologues of Dm stau are expressed in germ cells in both male and female mice, consistent with a role for these RNA binding proteins in mammalian gametogenesis.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Saunders PT,Pathirana S,Maguire SM,Doyle M,Wood T,Bownes Mdoi
10.1093/molehr/6.11.983subject
Has Abstractpub_date
2000-11-01 00:00:00pages
983-91issue
11eissn
1360-9947issn
1460-2407journal_volume
6pub_type
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journal_title:Molecular human reproduction
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journal_title:Molecular human reproduction
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journal_title:Molecular human reproduction
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pub_type: 杂志文章,评审
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doi:10.1093/molehr/gah022
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gal021
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah155
更新日期:2005-03-01 00:00:00
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journal_title:Molecular human reproduction
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doi:10.1093/molehr/gam079
更新日期:2008-01-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/molehr/7.11.1039
更新日期:2001-11-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/3.6.507
更新日期:1997-06-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaaa070
更新日期:2020-12-10 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gar010
更新日期:2011-07-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah172
更新日期:2005-05-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/2.11.815
更新日期:1996-11-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gap083
更新日期:2010-02-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
doi:10.1093/molehr/gat039
更新日期:2013-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/molehr/6.7.627
更新日期:2000-07-01 00:00:00
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journal_title:Molecular human reproduction
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更新日期:2010-10-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/2.5.335
更新日期:1996-05-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah041
更新日期:2004-05-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
doi:10.1093/molehr/gaq059
更新日期:2010-11-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah141
更新日期:2005-02-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/8.8.702
更新日期:2002-08-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau095
更新日期:2015-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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journal_title:Molecular human reproduction
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更新日期:2015-06-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah059
更新日期:2004-06-01 00:00:00