Abstract:
:Psoriasin is a small calcium-binding protein first found in psoriatic lesions and also up-regulated in other inflammatory skin diseases and cancer tissues. Psoriasin is also present in the fetal epithelial cells. Its biological function is unclear, but there is both in vitro and in vivo evidence for its chemokine-like activity. The aim of the present study was to find whether psoriasin could be found in the amniotic fluid and thus could have long-range immunobiological effects. Two recombinant psoriasins were prepared, one in Escherichia coli, the other one in Pichia pastoris. The former was used to produce a rabbit antiserum against psoriasin. Fractionation of full-term amniotic fluids with polyacrylamide gel electrophoresis (PAGE) and gel filtration associated with immunodetection with the antiserum were used to identify a protein compatible with the size of psoriasin. The identity of psoriasin was further verified by mass spectrometric analysis. Expression of psoriasin in cells of the amniotic membranes was detected with nested RT-PCR. Because of its chemokine-like activity, psoriasin present in the amniotic fluid might have consequential immunobiological effects during the fetal development.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Porre S,Heinonen S,Mäntyjärvi R,Rytkönen-Nissinen M,Perola O,Rautiainen J,Virtanen Tdoi
10.1093/molehr/gah141subject
Has Abstractpub_date
2005-02-01 00:00:00pages
87-92issue
2eissn
1360-9947issn
1460-2407pii
gah141journal_volume
11pub_type
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