Cell death and its suppression in human ovarian tissue culture.

Abstract:

:In women with premature ovarian failure, fertility may be preserved by ovarian tissue culture in vitro. However, techniques for tissue culture and follicle maturation have remained suboptimal. Our aim was to characterize ovarian tissue degeneration in cultures and to establish a model for cell death research in cultured ovarian tissue. Precise knowledge on the process resulting in cell death in cultured ovarian tissue will ultimately facilitate work aimed at improving long-term culture conditions. Ovarian tissue apoptosis was studied in a serum-free culture model in which nuclear DNA fragmentation was shown to occur within 24 h of the start of the culture. Activation of caspase-3 was detected in some stromal cells and a few oocytes. Since not all of the tissue exhibited signs of apoptosis and since DNA fragmentation increased over time, the tissue probably gradually dies by apoptosis. The antioxidant N-acetyl-L-cysteine (NAC; 25, 50 and 100 mmol/l) was found to inhibit this apoptosis. Thus, apoptosis appears to play a critical role in the degeneration of human ovarian cortical tissue cultures, and this cell death can be suppressed by NAC. The present tissue culture model can be used for identifying components capable of inhibiting cell death in vitro.

journal_name

Mol Hum Reprod

authors

Otala M,Erkkilä K,Tuuri T,Sjöberg J,Suomalainen L,Suikkari AM,Pentikäinen V,Dunkel L

doi

10.1093/molehr/8.3.228

subject

Has Abstract

pub_date

2002-03-01 00:00:00

pages

228-36

issue

3

eissn

1360-9947

issn

1460-2407

journal_volume

8

pub_type

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