Abstract:
:Embryo implantation and subsequent decidualization, trophoblast invasion and formation of a functional placenta are crucial for establishment and maintenance of pregnancy. Interleukin-11 signalling has been shown to be obligatory for adequate decidualization and trophoblast invasion in mice. Defects in IL-11 signalling in mice result in trophoblast over-invasion and fetal loss. The pathological situation of human tubal pregnancy resembles that of IL-11Ralpha(-/-) mice concerning these symptoms. As our interest is focused on the human early pregnancy, we compared IL-11 expression at the implantation site of ectopic tubal pregnancy (EP) to 1st and 2nd trimester of normal intrauterine pregnancies (IP), and to the normal cycling endometrium. The mRNA expression of IL-11 and IL-11Ralpha was analysed by semiquantitative RT-PCR. Protein expression was detected by western blotting and immunohistochemistry. IL-11Ralpha is expressed constitutively in all tissue specimens analysed. IL-11 is expressed predominantly during follicular and early luteal phase of the menstrual cycle. In IP, IL-11 expression peaks during the 1st trimester and declines from the beginning of the 2nd trimester onwards. In tubal abortions, IL-11 expression is reduced in comparison to vital EP and IP. Cultured primary endometrial and decidual epithelial cells were analysed for hormonal regulation of IL-11 by enzyme-linked immunosorbent assay and RT-PCR. IL-11 is up-regulated by estrogen and down-regulated by progesterone. Overall, our results indicate that in humans, IL-11 signalling is significantly involved in regulation of trophoblast invasion. In the case of tubal abortion, inadequate IL-11 signalling may therefore result in dysregulation of trophoblast invasion.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
von Rango U,Alfer J,Kertschanska S,Kemp B,Müller-Newen G,Heinrich PC,Beier HM,Classen-Linke Idoi
10.1093/molehr/gah107subject
Has Abstractpub_date
2004-11-01 00:00:00pages
783-92issue
11eissn
1360-9947issn
1460-2407pii
gah107journal_volume
10pub_type
杂志文章abstract:STUDY QUESTION:Can enzymatically dispersed testicular cells from adult men reassemble into seminiferous cord-like structures in vitro? SUMMARY ANSWER:Adult human testicular somatic cells reassembled into testicular cord-like structures via dynamic interactions of Sertoli and peritubular cells. WHAT IS KNOWN ALREADY:I...
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更新日期:1996-08-01 00:00:00
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更新日期:2015-02-01 00:00:00
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更新日期:1998-11-01 00:00:00
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更新日期:2003-08-01 00:00:00
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更新日期:2016-04-01 00:00:00
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更新日期:2015-08-01 00:00:00
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更新日期:2008-06-01 00:00:00
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更新日期:2015-07-01 00:00:00
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