Abstract:
:In addition to prostaglandins, inflammatory cytokines induce uterine contraction via oxytocin (OT). Placental leucine aminopeptidase (P-LAP), an oxytocinase that is identical to cystine aminopeptidase, destroys OT activity. Patients with spontaneous preterm delivery have higher concentrations of inflammatory cytokines and lower P-LAP activities than those with normal delivery. In addition, the P-LAP promoter region contains putative binding sites for cytokine-induced transcription factors. We therefore postulated that inflammatory cytokines suppress P-LAP expression and examined this notion using BeWo choriocarcinoma cells cultured in the presence of cytokines. However, interleukin-1beta (IL-1beta) increased P-LAP activity in a time- and dose-dependent manner. Furthermore, Western blot analysis showed a dose-dependent increase of P-LAP proteins. We also detected IL-1 type I receptor mRNA in BeWo cells by RT-PCR. Semi-quantitative RT-PCR and Southern blot analysis showed that IL-1beta also increased P-LAP mRNA, which was abrogated by prior exposure to cycloheximide. Luciferase assays did not reveal any regulatory regions that could explain IL-1beta-induced P-LAP mRNA accumulation within 1.1 kb upstream of the P-LAP gene. Immunohistochemical analysis of human placenta with chorioamnionitis demonstrated prominent P-LAP staining at sites of abundant inflammatory cell infiltration. These findings indicated that prolonged exposure to IL-1beta induces P-LAP in the trophoblasts, possibly via other de-novo protein synthesis, which contradicted our initial hypothesis.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Ikoma Y,Nomura S,Ito T,Katsumata Y,Nakata M,Iwanaga K,Okada M,Kikkawa F,Tamakoshi K,Nagasaka T,Tsujimoto M,Mizutani Sdoi
10.1093/molehr/gag015subject
Has Abstractpub_date
2003-02-01 00:00:00pages
103-10issue
2eissn
1360-9947issn
1460-2407journal_volume
9pub_type
杂志文章abstract::Follicular prostaglandin concentrations increase following the gonadotrophin surge in domestic animals and rodents approximately 10 h before follicle rupture, suggesting a unifying role for prostaglandins in the timing of ovulation. However, little is known about prostaglandin production by the primate ovulatory folli...
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更新日期:1997-03-01 00:00:00
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更新日期:2000-01-01 00:00:00
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更新日期:2014-03-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
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更新日期:2003-05-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
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journal_title:Molecular human reproduction
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更新日期:2019-04-01 00:00:00
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journal_title:Molecular human reproduction
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更新日期:2017-12-01 00:00:00
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更新日期:2014-08-01 00:00:00
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更新日期:2014-05-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/molehr/3.10.827
更新日期:1997-10-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00