Global gene analysis of oocytes from early stages in human folliculogenesis shows high expression of novel genes in reproduction.

abstract：:The human egg may only be fertilized by one spermatozoon to prevent polyploidy. In most mammals, the primary block to polyspermy occurs at the zona pellucida (ZP). Little is known of the human ZP and the changes occurring following fertilization to prevent polyploidy. Using antibodies directed against synthetic peptid...

journal_title：Molecular human reproduction

authors： Bauskin AR,Franken DR,Eberspaecher U,Donner P

更新日期：1999-06-01 00:00:00

abstract：:The aim of the present study was to investigate the potential role of Toll-like receptor 4 (TLR4) in lipopolysaccharide (LPS)-induced preterm delivery. Intraperitoneal injection of LPS in the presence or absence of previous TLR4 blockade was performed to establish a murine model of preterm delivery. The incidences of ...

journal_title：Molecular human reproduction

authors： Li L,Kang J,Lei W

更新日期：2010-04-01 00:00:00

abstract：:In early gestation, the human placental renin-angiotensin system (RAS) is upregulated and plays a role in placental development. Among other functions, signalling through the angiotensin II type 1 receptor (AT1R) initiates proliferation. Many microRNAs (miRNAs) targeting placental RAS mRNAs are downregulated at this t...

journal_title：Molecular human reproduction

authors： Arthurs AL,Lumbers ER,Pringle KG

更新日期：2019-04-01 00:00:00

abstract：:Prevention of mitochondrial DNA (mtDNA) diseases may currently be possible using germline nuclear transfer (NT). However, scientific evidence to compare efficiency of different NT techniques to overcome mtDNA diseases is lacking. Here, we performed four types of NT, including first or second polar body transfer (PB1/2...

journal_title：Molecular human reproduction

authors： Tang M,Guggilla RR,Gansemans Y,Van der Jeught M,Boel A,Popovic M,Stamatiadis P,Ferrer-Buitrago M,Thys V,Van Coster R,Deforce D,De Sutter P,Van Nieuwerburgh F,Heindryckx B

更新日期：2019-12-01 00:00:00

abstract：:The Prader-Willi (PWS) and Angelman (AS) syndromes are two clinically distinct syndromes which result from lack of expression of imprinted genes within chromosome 15q11-q13. These two syndromes result from 15q11-q13 deletions, chromosome 15 uniparental disomy (UPD), imprinting centre mutations and, for AS, probable mu...

journal_title：Molecular human reproduction

authors： Glenn CC,Driscoll DJ,Yang TP,Nicholls RD

更新日期：1997-04-01 00:00:00

abstract：:In oocytes, cytoplasmic 3' polyadenylation regulates translational activation of dormant mRNA during meiotic maturation. Thus exogenous proteins are hardly expressed after injection of conventional RNA. To circumvent this, we synthesized a long polyadenylated (approximately 250 A) tail to encode RNA with an enhanced y...

journal_title：Molecular human reproduction

authors： Aida T,Oda S,Awaji T,Yoshida K,Miyazaki S

更新日期：2001-11-01 00:00:00

abstract：:Within the context of an oocyte in vitro maturation (IVM) program for reproductive treatment, oocyte cumulus complexes (COCs) derived from follicles <6 mm in patients with PCOS were matured in vitro. Key transcripts related to meiotic maturation (FSHR, LHCGR, EGFR, PGR) and oocyte competence (AREG, ADAMTS, HAS2, PTGS2...

journal_title：Molecular human reproduction

authors： Guzman L,Adriaenssens T,Ortega-Hrepich C,Albuz FK,Mateizel I,Devroey P,De Vos M,Smitz J

更新日期：2013-01-01 00:00:00

abstract：:Preimplantation factor (PIF) is detected in the serum of women shortly after fertilization; its origin, however, has not been established. In this study, the embryonal origin of PIF was investigated and partial characterization of the factor was carried out. Culture media from viable human 2-8-cell stage embryos and m...

journal_title：Molecular human reproduction

authors： Roussev RG,Coulam CB,Kaider BD,Yarkoni M,Leavis PC,Barnea ER

更新日期：1996-11-01 00:00:00

abstract：:G12 monoclonal antibody (mAb), one of a library of constructed mAb directed against human sperm proteins, was found by immunoperoxidase staining to label the post-acrosomal and neck regions of fixed human cauda epididymal and ejaculated spermatozoa. Epithelium and fluid of caput epididymis were strongly labelled while...

journal_title：Molecular human reproduction

authors： Lefèvre A,Martin Ruiz C,Chokomian S,Duquenne C,Finaz C

更新日期：1997-06-01 00:00:00

abstract：STUDY HYPOTHESIS:Does a preferential X chromosome inactivation (XCI) pattern exist in female human pluripotent stem cells (hPSCs) and does the pattern change during long-term culture or upon differentiation? STUDY FINDING:We identified two independent phenomena that lead to aberrant XCI patterns in female hPSC: a rapi...

journal_title：Molecular human reproduction

authors： Geens M,Seriola A,Barbé L,Santalo J,Veiga A,Dée K,Van Haute L,Sermon K,Spits C

更新日期：2016-04-01 00:00:00

abstract：:This study assesses the role of progesterone receptor membrane component 1 (PGRMC1) in actions of progesterone (P4) on human myometrium during pregnancy and labour. Myometrial tissues were obtained from non-pregnant patients during hysterectomy or pregnant women undergoing C-section at term and preterm, before and dur...

journal_title：Molecular human reproduction

authors： Wu W,Shi SQ,Huang HJ,Balducci J,Garfield RE

更新日期：2011-04-01 00:00:00

abstract：:Oocyte quality is one of the most important aspects of in vitro embryo development. Extensive epigenetic programming must occur during oocyte growth and maturation. A specific DNA methylation pattern of the imprinted genes must be established on differentially methylated regions (DMR). The insulin-like growth factor 2...

journal_title：Molecular human reproduction

authors： Fagundes NS,Michalczechen-Lacerda VA,Caixeta ES,Machado GM,Rodrigues FC,Melo EO,Dode MA,Franco MM

更新日期：2011-02-01 00:00:00

abstract：:Tenascin-C is an extracellular matrix glycoprotein whose monomers include eight consecutive fibronectin type III-like repeats, encoded by exons 10-16, and which are subject to alternative splicing. Transcripts containing these exons are expressed during tissue wounding and active tissue remodelling. Human fetal membra...

journal_title：Molecular human reproduction

authors： Bell SC,Pringle JH,Taylor DJ,Malak TM

更新日期：1999-11-01 00:00:00

abstract：:E-cadherin (uvomorulin)-mediated cell interactions are essential for preimplantation development in mammals. We observed that E-cadherin is expressed at contact sites between blastomeres of 2-cell mouse embryos of non-blocking genotype (CBA x C57BL F1) explanted at 32 h post human chorionic gonadotrophin (HCG) and cul...

journal_title：Molecular human reproduction

authors： Neganova IE,Sekirina GG,Eichenlaub-Ritter U

更新日期：2000-05-01 00:00:00

abstract：:Endometriosis, the presence of ectopic endometrial tissue outside the uterine cavity, is a common disease affecting women during their reproductive years. The aim of this study was to identify the molecular mechanism of transcriptional regulation of inflammatory cyclooxygenase-2 (COX-2) gene during endometriosis by hy...

journal_title：Molecular human reproduction

authors： Kim KH,Kim HY,Kim HH,Lee KS,Cheong J

更新日期：2011-11-01 00:00:00

abstract：:In humans, male and female partners contribute more or less equally to the infertility problem. In approximately 20% of infertile couples, the concurrence of male and female factors is suggested to be responsible for infertility. Neither of these factors are known nor is there a model system to prove this assumption. ...

journal_title：Molecular human reproduction

authors： Nayernia K,Adham IM,Shamsadin R,Müller C,Sancken U,Engel W

更新日期：2002-05-01 00:00:00

abstract：:The two estrogen receptors, ERalpha and ERbeta, are likely to have roles in the pathophysiology of fibroid development. They have been detected in myometrial and leiomyoma (fibroid) tissue, but the cell types expressing ERalpha and ERbeta have not been determined. ERs have also been detected in human endothelial cells...

journal_title：Molecular human reproduction

authors： Gargett CE,Bucak K,Zaitseva M,Chu S,Taylor N,Fuller PJ,Rogers PA

更新日期：2002-08-01 00:00:00

abstract：:In the interest of classifying cases of male factor infertility, we have paid particular attention to the sugar ligand binding properties of the human sperm surface and the functional capacity of the acrosome for exocytosis--key parameters for assessing sperm fertilizing ability. Zona recognition and binding involve t...

journal_title：Molecular human reproduction

authors： Benoff S,Hurley IR,Mandel FS,Cooper GW,Hershlag A

更新日期：1997-10-01 00:00:00

abstract：:We report here the molecular cloning and characterization of human haspin cDNA and its genomic DNA construct. The haspin protein is a unique protein kinase, first isolated from mouse testis. Specifically expressed in mouse testicular germ cells, haspin is suggested to play a role in cell cycle arrest in haploid sperma...

journal_title：Molecular human reproduction

authors： Tanaka H,Iguchi N,Nakamura Y,Kohroki J,de Carvalho CE,Nishimune Y

更新日期：2001-03-01 00:00:00

abstract：:The human endometrium is a cyclically regenerating organ under the influence of ovarian steroid hormones. Disturbances in this highly coordinated regulation of endometrial proliferation and differentiation may result in infertility and diseases such as endometriosis and endometrial cancer. Environmental toxins belongi...

journal_title：Molecular human reproduction

authors： Willing C,Peich M,Danescu A,Kehlen A,Fowler PA,Hombach-Klonisch S

更新日期：2011-02-01 00:00:00

abstract：:Preimplantation development shifts from a maternal to embryonic programme rapidly after fertilization. Although the majority of oogenetic products are lost during the maternal to embryonic transition (MET), several do survive this interval to contribute directly to supporting preimplantation development. Embryonic gen...

journal_title：Molecular human reproduction

authors： Bell CE,Calder MD,Watson AJ

更新日期：2008-12-01 00:00:00

abstract：:During pregnancy, a cervical connective tissue remodelling takes place, clinically recognized as softening, effacement and dilatation. The role of oestrogens and their receptors (ER) in this process is not clear. ERalpha is a ligand-activated transcription factor involved in many physiological processes. The identific...

journal_title：Molecular human reproduction

authors： Wang H,Stjernholm Y,Ekman G,Eriksson H,Sahlin L

更新日期：2001-03-01 00:00:00

abstract：:We have developed a competitive reverse transcription-polymerase chain reaction (RT-PCR) sensitive enough to detect and quantify as little as 2-fold differences in gene expression in individual oocytes and embryos throughout human preimplantation development. This RT-PCR assay can be tailored for the examination of an...

journal_title：Molecular human reproduction

authors： Taylor DM,Handyside AH,Ray PF,Dibb NJ,Winston RM,Ao A

更新日期：2001-02-01 00:00:00

abstract：:After meiosis, during the final stages of spermatogenesis, the haploid male genome undergoes major structural changes, resulting in a shift from a nucleosome-based genome organization to the sperm-specific, highly compacted nucleoprotamine structure. Recent data support the idea that region-specific programming of the...

journal_title：Molecular human reproduction

authors： Govin J,Gaucher J,Ferro M,Debernardi A,Garin J,Khochbin S,Rousseaux S

更新日期：2012-01-01 00:00:00

abstract：:In male mice heterozygous for a null apolipoprotein B (apoB), allele infertility was noticed. These data led us to investigate a possible role of APOB gene polymorphism and male infertility in humans. In this case-control study, we searched for an association between the insertion/deletion (I/D) polymorphism of the AP...

journal_title：Molecular human reproduction

authors： Peterlin B,Zorn B,Volk M,Kunej T

更新日期：2006-12-01 00:00:00

abstract：:Granzyme B (graB) plays a pivotal role in cytotoxic lymphocyte granule-mediated apoptosis through cleavage of intracellular proteins in target cells. Proteinase inhibitor-9 (PI-9) is a potent inhibitor of graB and is highly expressed in cytotoxic lymphocytes. Here, we show by immunohistochemistry that PI-9 is also abu...

journal_title：Molecular human reproduction

authors： Hirst CE,Buzza MS,Sutton VR,Trapani JA,Loveland KL,Bird PI

更新日期：2001-12-01 00:00:00

abstract：:Type I inositol 1,4,5-trisphosphate-sensitive receptors (InsP(3)R) are expressed in human oocytes and may be involved in operating the Ca(2+) release triggered by the fertilizing sperm. This study examines the contribution of type I InsP(3)R in operating Ca(2+) release in human oocytes secondary to InsP(3) itself, usi...

journal_title：Molecular human reproduction

authors： Goud PT,Goud AP,Leybaert L,Van Oostveldt P,Mikoshiba K,Diamond MP,Dhont M

更新日期：2002-10-01 00:00:00

abstract：:Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were ...

journal_title：Molecular human reproduction

authors： Harris SD,Anobile CJ,McLoughlin JD,Mitchell R,Lambert A,Robertson WR

更新日期：1996-10-01 00:00:00

abstract：:Early embryonic development is characterized by drastic changes in chromatin structure that affects the accessibility of the chromatin. In human, the chromosome reorganization and its involvement in the first linage segregation are poorly characterized due to the difficulties in obtaining human embryonic material and ...

journal_title：Molecular human reproduction

authors： Yang M,Tao X,Titus S,Zhao T,Scott RT,Seli E

更新日期：2020-09-01 00:00:00

abstract：:Charcot-Marie-Tooth (CMT) disease type 1A is an autosomal dominant peripheral neuropathy characterized by slow progressive distal muscle wasting and weakness, and decreased nerve conduction velocities. Most CMT1A cases (>98%) are caused by a duplication of a 1.5 Mb region on the short arm of chromosome 17 containing t...

journal_title：Molecular human reproduction

authors： De Vos A,Sermon K,Van de Velde H,Joris H,Vandervorst M,Lissens W,Mortier G,De Sutter P,Löfgren A,Van Broeckhoven C,Liebaers I,Van Steirteghem A

更新日期：1998-10-01 00:00:00