Sperm nitric oxide and motility: the effects of nitric oxide synthase stimulation and inhibition.

Abstract:

:Nitric oxide (NO) is synthesized from L-arginine by a family of enzymes known as the nitric oxide synthases (NOS). We have recently shown a NOS similar to constitutive brain NOS (bNOS) and endothelial NOS (ecNOS) to be present in spermatozoa. The aim of this study is to investigate NO production by human spermatozoa and the effects of stimulation and inhibition of NOS. This was carried out using the Iso-NO, an isolated NO meter and sensor, which provides rapid, accurate and direct measurements of NO. Semen samples with normozoospermic and asthenozoospermic profiles were prepared using a direct swim-up technique. Basal concentrations of NO and stimulated NO production were measured after exposure to the calcium ionophore (A23187; 0.01-10 microM) a potent activator of constitutive NOS. NO production in human spermatozoa was significantly increased by the addition of A23187 30 seconds after stimulation. Furthermore, this response was greatly diminished by pre-incubating the samples with competitive inhibitors of L-arginine, the substrate for NOS, before treatment with calcium ionophore. In the presence of N(G)-nitro-L-arginine methyl ester (L-NAME), N(G)-nitro-L-arginine (L-NA) or N(G)-methyl-L-arginine (L-NMMA; all at 10 microM), NO production was inhibited with a rank order of potency L-NAME > L-NMMA > L-NA which is in accordance with the inhibition of an endothelial type of constitutive NOS.

journal_name

Mol Hum Reprod

authors

Donnelly ET,Lewis SE,Thompson W,Chakravarthy U

doi

10.1093/molehr/3.9.755

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

755-62

issue

9

eissn

1360-9947

issn

1460-2407

journal_volume

3

pub_type

杂志文章
  • Relationship between abnormal sperm chromatin packing and IVF results.

    abstract::This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packi...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/5.9.825

    authors: Filatov MV,Semenova EV,Vorob'eva OA,Leont'eva OA,Drobchenko EA

    更新日期:1999-09-01 00:00:00

  • Analysis of accessible chromatin landscape in the inner cell mass and trophectoderm of human blastocysts.

    abstract::Early embryonic development is characterized by drastic changes in chromatin structure that affects the accessibility of the chromatin. In human, the chromosome reorganization and its involvement in the first linage segregation are poorly characterized due to the difficulties in obtaining human embryonic material and ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gaaa048

    authors: Yang M,Tao X,Titus S,Zhao T,Scott RT,Seli E

    更新日期:2020-09-01 00:00:00

  • High concentrations of the vascular endothelial growth factor and interleukin-8 in ovarian endometriomata.

    abstract::Patients with endometriosis are characterized by the ability of the endometrium to implant and by the peritoneal response to the tissue; angiogenic factors may play a significant role in the aetiology of endometriosis supporting the implantation of ectopic endometrial cells. Vascular endothelial growth factor (VEGF) i...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/6.1.50

    authors: Fasciani A,D'Ambrogio G,Bocci G,Monti M,Genazzani AR,Artini PG

    更新日期:2000-01-01 00:00:00

  • Developmental origins of inflammatory and immune diseases.

    abstract::Epidemiological and experimental animal studies show that suboptimal environments in fetal and neonatal life exert a profound influence on physiological function and risk of diseases in adult life. The concepts of the 'developmental programming' and Developmental Origins of Health and Diseases (DOHaD) have become well...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章,评审

    doi:10.1093/molehr/gaw036

    authors: Chen T,Liu HX,Yan HY,Wu DM,Ping J

    更新日期:2016-08-01 00:00:00

  • Identification of genes differentially expressed in testes containing carcinoma in situ.

    abstract::Virtually all testicular germ cell tumours originate from a common precursor, the carcinoma in situ (CIS) cell. The precise nature of the molecular mechanisms leading to CIS remains largely unknown. We performed the first systematic analysis of gene expression in testis with CIS compared to normal testis by the differ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gah059

    authors: Hoei-Hansen CE,Nielsen JE,Almstrup K,Hansen MA,Skakkebaek NE,Rajpert-DeMeyts E,Leffers H

    更新日期:2004-06-01 00:00:00

  • Expression of a testis-specific member of the olfactory receptor gene family in human primordial germ cells.

    abstract::Olfactory receptors are G protein-coupled transmembrane receptors. Genes encoding olfactory receptors constitute a large gene family of approximately 1000 total member genes. In mammals, a subset of member genes is specifically expressed in the testis (and not in the olfactory mucosa) and olfactory receptor proteins h...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/7.6.553

    authors: Goto T,Salpekar A,Monk M

    更新日期:2001-06-01 00:00:00

  • Sexual selection and the evolution of sperm quality.

    abstract::Sperm experience intense and varied selection that dramatically impacts the evolution of sperm quality. Selection acts to ensure that sperm are fertilization-competent and able to overcome the many challenges experienced on their way towards eggs. However, simply being able to fertilize an egg is not enough to ensure ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gau067

    authors: Fitzpatrick JL,Lüpold S

    更新日期:2014-12-01 00:00:00

  • The molecular basis of recurrent pregnancy loss: impaired natural embryo selection.

    abstract::Recurrent pregnancy loss (RPL) is a common and distressing disorder. Chromosomal errors in the embryo are the single most common cause, whereas uterine factors are invariably invoked to explain non-chromosomal miscarriages. These uterine factors are, however, poorly defined. The ability of a conceptus to implant in th...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章,评审

    doi:10.1093/molehr/gaq079

    authors: Teklenburg G,Salker M,Heijnen C,Macklon NS,Brosens JJ

    更新日期:2010-12-01 00:00:00

  • Sex-related physiology of the preimplantation embryo.

    abstract::Male and female preimplantation mammalian embryos differ not only in their chromosomal complement, but in their proteome and subsequent metabolome. This phenomenon is due to a finite period during preimplantation development when both X chromosomes are active, between embryonic genome activation and X chromosome inact...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章,评审

    doi:10.1093/molehr/gaq042

    authors: Gardner DK,Larman MG,Thouas GA

    更新日期:2010-08-01 00:00:00

  • Prostaglandin E2 induces inhibin alpha- and beta A-subunit mRNA and secretion of dimeric inhibin A in cultured human granulosa-luteal cells.

    abstract::Prostaglandins (PG) E2 and F2 alpha are produced by the human corpus luteum in significant amounts and they are known to exert local luteotrophic and luteolytic effects respectively. The present study determined how PGE2 and PGF2 alpha are involved in the regulation of inhibin A, a granulosa cell-derived glycoprotein ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/2.11.815

    authors: Erämaa M,Ritvos O

    更新日期:1996-11-01 00:00:00

  • Evaluation of stimulus-induced acrosome reaction by two-colour flow cytometric analysis.

    abstract::Acrosome status in human spermatozoa from 20 normozoospermic men was evaluated by flow cytometry following the induction of the acrosome reaction with the ionophore A23187. Dual fluorescence staining of methanol fixed spermatozoa incubated with and without (control) the ionophore A23187 was performed with probes which...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/4.3.243

    authors: Nikolaeva MA,Golubeva EL,Kulakov VI,Sukhikh GT

    更新日期:1998-03-01 00:00:00

  • Biochemical study of individual zonae from human oocytes that failed to undergo fertilization in intracytoplasmic sperm injection.

    abstract::Successful intracytoplasmic sperm injection (ICSI) is dependent upon the competence of the oocyte to respond to the injection of a spermatozoon in the presence of calcium. This study determined if oocytes that failed to become fertilized (absence of any pronuclei) failed to undergo cytoplasmic activation, as ascertain...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/2.12.959

    authors: Gross V,Dubey A,Penzias AS,Layman L,Reindollar R,Ducibella T

    更新日期:1996-12-01 00:00:00

  • Regulation of placental monocyte chemotactic and activating factor during pregnancy and chorioamnionitis.

    abstract::A number of placental cytokines participate in the feto-maternal defence mechanism. Monocyte chemotactic and activating factor (MCAF) is one of these chemokines. We investigated the pattern of placental MCAF production, the localization of MCAF-producing cells in the placenta, and alterations in its expression in chor...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/4.4.393

    authors: Shimoya K,Matsuzaki N,Sawai K,Saji F,Murata Y,Yasumoto K,Su S,Mukaida N,Matsushima K

    更新日期:1998-04-01 00:00:00

  • Gene expression and tissue concentrations of IGF-I in human myometrium and fibroids under different hormonal conditions.

    abstract::The expression of insulin-like growth factor-I (IGF-I) was measured at the mRNA and protein level in myometrium and fibroids from women with and without preoperative treatment with a gonadotrophin-releasing hormone (GnRH) agonist for 3 months, from post-menopausal women, from pregnant women and in myometrium from wome...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/6.10.915

    authors: Englund K,Lindblom B,Carlström K,Gustavsson I,Sjöblom P,Blanck A

    更新日期:2000-10-01 00:00:00

  • Fas-associated factor (FAF1) is required for the early cleavage-stages of mouse embryo.

    abstract::FAF1 was initially isolated as a Fas-associated factor and was subsequently found to interact with a subset of additional proteins that are involved in many cellular events including Fas-mediated apoptosis, heat shock signalling pathways and ubiquitin-dependent processes. Here, we describe that the 74-kDa FAF1 is ubiq...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gan009

    authors: Adham IM,Khulan J,Held T,Schmidt B,Meyer BI,Meinhardt A,Engel W

    更新日期:2008-04-01 00:00:00

  • RNA in the ejaculate spermatozoon: a window into molecular events in spermatogenesis and a record of the unusual requirements of haploid gene expression and post-meiotic equilibration.

    abstract::Several reports demonstrating the presence of messenger RNAs for sperm-specific nucleoproteins, the protamines PRM-1, PRM-2, and the transition protein TP-1 as well as beta-actin, c-MYC, HLA1, and beta 1 integrin have challenged the accepted view of the transcriptional dormance of terminally differentiated spermatozoa...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章,评审

    doi:10.1093/molehr/3.8.669

    authors: Miller D

    更新日期:1997-08-01 00:00:00

  • Oct-4 mRNA and protein expression during human preimplantation development.

    abstract::The transcription factor OCT-4 is regarded as a critical factor in controlling mammalian early embryonic development because of its role in toti-/pluripotency. In human preimplantation embryos, OCT-4 studies are limited to RNA analysis of abnormally developing embryos. This study thoroughly investigated the expression...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gah155

    authors: Cauffman G,Van de Velde H,Liebaers I,Van Steirteghem A

    更新日期:2005-03-01 00:00:00

  • Regulated expression of cytokines in human endometrium throughout the menstrual cycle: dysregulation in habitual abortion.

    abstract::It is widely assumed that, after ovulation, the human endometrium undergoes specific changes and becomes receptive to the implantation of embryo during the mid-secretory phase. When implantation does not take place, further changes occur which eventually result in the shedding of human endometrium. The present study w...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/6.7.627

    authors: von Wolff M,Thaler CJ,Strowitzki T,Broome J,Stolz W,Tabibzadeh S

    更新日期:2000-07-01 00:00:00

  • The effect of intrauterine levonorgestrel use on the expression of c-JUN, oestrogen receptors, progesterone receptors and Ki-67 in human endometrium.

    abstract::The major regulators of endometrial function are oestrogen and progesterone, which act through binding their nuclear receptors and by activating transcription of their target genes. Interactions between steroid receptors and transcription proteins, e.g. c-JUN/AP-1, can modulate steroid action at the transcriptional le...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/4.12.1110

    authors: Salmi A,Pakarinen P,Peltola AM,Rutanen EM

    更新日期:1998-12-01 00:00:00

  • Tumour necrosis factor-alpha (TNF-alpha) in human endometrium and uterine secretion: an evaluation by immunohistochemistry, ELISA and semiquantitative RT-PCR.

    abstract::Tumour necrosis factor-alpha (TNF-alpha is a pleiotropic cytokine synthesized throughout the female reproductive tract. Even though evidence has accumulated that supports its role in autocrine and paracrine processes, its expression and function in the human endometrium are still not completely understood. To gain a b...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/5.2.146

    authors: von Wolff M,Classen-Linke I,Heid D,Krusche CA,Beier-Hellwig K,Karl C,Beier HM

    更新日期:1999-02-01 00:00:00

  • Tyrosine nitration in human spermatozoa: a physiological function of peroxynitrite, the reaction product of nitric oxide and superoxide.

    abstract::Tyrosine nitration is a widely used marker of peroxynitrite (ONOO-) produced from the reaction of nitric oxide (NO.) with superoxide (O2(.-)). Since human spermatozoa are able to produce both NO. and O2(.-) during capacitation in vitro, we investigated whether spontaneous tyrosine nitration of proteins occurs in human...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/7.10.913

    authors: Herrero MB,de Lamirande E,Gagnon C

    更新日期:2001-10-01 00:00:00

  • IDO expression on decidual and peripheral blood dendritic cells and monocytes/macrophages after treatment with CTLA-4 or interferon-gamma increase in normal pregnancy but decrease in spontaneous abortion.

    abstract::Recent data demonstrated that CD4+CD25+ regulatory T (Treg) cells and an enzyme called indoleamine 2,3-dioxygenase (IDO) mediate maternal tolerance to the fetus. Interestingly, Treg cells express the CTLA-4 molecule on their surface, and B7 (CD80/86) ligation by CTLA-4 enhanced IDO activity of dendritic cells (DCs) an...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gah246

    authors: Miwa N,Hayakawa S,Miyazaki S,Myojo S,Sasaki Y,Sakai M,Takikawa O,Saito S

    更新日期:2005-12-01 00:00:00

  • The effect of maternal high-fat/high-sugar diet on offspring oocytes and early embryo development.

    abstract::Observational human data and several lines of animal experimental data indicate that maternal obesity impairs offspring health. Here, we comprehensively tested the model that maternal obesity causes defects in the next three generations of oocytes and embryos. We exposed female F0 mice to a high-fat/high-sugar (HF/HS)...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gaz049

    authors: Andreas E,Reid M,Zhang W,Moley KH

    更新日期:2019-11-30 00:00:00

  • Pregnancy after preimplantation genetic diagnosis for Charcot-Marie-Tooth disease type 1A.

    abstract::Charcot-Marie-Tooth (CMT) disease type 1A is an autosomal dominant peripheral neuropathy characterized by slow progressive distal muscle wasting and weakness, and decreased nerve conduction velocities. Most CMT1A cases (>98%) are caused by a duplication of a 1.5 Mb region on the short arm of chromosome 17 containing t...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/4.10.978

    authors: De Vos A,Sermon K,Van de Velde H,Joris H,Vandervorst M,Lissens W,Mortier G,De Sutter P,Löfgren A,Van Broeckhoven C,Liebaers I,Van Steirteghem A

    更新日期:1998-10-01 00:00:00

  • Rapid chromosomal analysis of germ-line cells by FISH: an investigation of an infertile male with large-headed spermatozoa.

    abstract::A rapid fluorescence in-situ hybridization (FISH) technique was used for direct chromosomal analysis on germ cells from an infertile male with large-headed spermatozoa. The interphase chromosomes were fluorescently-labelled using an extremely bright cyanine dye during a 5-15 min FISH procedure. Germ cells were analyse...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/2.9.665

    authors: Yurov YB,Saias MJ,Vorsanova SG,Erny R,Soloviev IV,Sharonin VO,Guichaoua MR,Luciani JM

    更新日期:1996-09-01 00:00:00

  • Identification and characterization of an ascorbic acid transporter in human granulosa-lutein cells.

    abstract::Ascorbic acid serves a vital role as a pre-eminent antioxidant. In animals, it has been shown to be concentrated in granulosa and theca cells of the follicle, in luteal cells of the corpus luteum, and in the peripheral cytoplasm of the oocyte. We have previously identified hormonally-regulated ascorbic acid transporte...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/5.4.299

    authors: Zreik TG,Kodaman PH,Jones EE,Olive DL,Behrman H

    更新日期:1999-04-01 00:00:00

  • Identification and localization of G protein subunits in human spermatozoa.

    abstract::Antibodies to alpha and beta subunits of guanine nucleotide regulatory proteins (G proteins) were used to identify which G proteins are present in mature human spermatozoa and to determine their subcellular localization. Immunoblots of membranes from spermatozoa demonstrate the presence of Galphai2, Galphai3, Galphaq/...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/5.1.38

    authors: Merlet F,Weinstein LS,Goldsmith PK,Rarick T,Hall JL,Bisson JP,de Mazancourt P

    更新日期:1999-01-01 00:00:00

  • Temporal expression of platelet-derived growth factor (PDGF)-A and its receptor in human preimplantation embryos.

    abstract::In order to improve assisted fertilization in humans it is important to elucidate the mechanisms of control of growth and development in the early pre-embryo. Increasing evidence shows that growth factors are of importance for such control mechanisms. As platelet-derived growth factor (PDGF) has been shown to enhance ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/2.7.507

    authors: Osterlund C,Wramsby H,Pousette A

    更新日期:1996-07-01 00:00:00

  • Developmental expression pattern of a novel gene, TSG23/Tsg23, suggests a role in spermatogenesis.

    abstract::A novel gene, TSG23/Tsg23, was identified by comparing the expression profiles of human adult and fetal testis using Affymetrix Genechips. RT-PCR analysis from multiple human and mouse tissues indicated TSG23/Tsg23 mRNA was mainly expressed in the testis. In situ hybridization revealed that TSG23/Tsg23 mRNA was locate...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gap015

    authors: Zhou Y,Qin D,Tang A,Zhou D,Qin J,Yan B,Diao R,Jiang Z,Cai Z,Gui Y

    更新日期:2009-04-01 00:00:00

  • Temporal patterns of gene regulation and upstream regulators contributing to major developmental transitions during Rhesus macaque preimplantation development.

    abstract::The preimplantation period of life in mammals encompasses a tremendous amount of restructuring and remodeling of the embryonic genome and reprogramming of gene expression. These vast changes support metabolic activation and cellular processes that drive early cleavage divisions and enable the creation of the earliest ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gaz001

    authors: Schall PZ,Ruebel ML,Midic U,VandeVoort CA,Latham KE

    更新日期:2019-03-01 00:00:00