Abstract:
:We purified by fractionation on 10-40% glycerol gradients, 26S proteasomes from normal human spermatozoa. These proteasomes, which participate in the ATP-dependent degradation of ubiquitinated proteins, share a similar sedimentation coefficient to those purified from other human tissues. Fluorogenic peptide assays reveal they have chymotrypsin, trypsin and peptidyl-glutamyl-like peptide hydrolysing activities; the chymotrypsin activity is ablated by the specific 26S proteasome inhibitor MG132. Confirmation that these large proteases are 26S proteasomes is provided by detection of the 20S proteasome subunits HC2, XAPC7, RN3 and Z and regulatory ATPases MSS1, TBP1, SUG1 and SUG2 by Western analyses with monoclonal antisera. These antigens are found only in the gradient fractions enriched in proteolytic activities. We have also shown that, although mature spermatozoa from mice have considerably reduced amounts of a ubiquitin-conjugating enzyme (E2) and ubiquitin-protein conjugates in comparison with less mature germ cells, they retain relatively high values of 26S proteasome activity. This suggests that proteasomes may have further roles to play in normal sperm physiology.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Tipler CP,Hutchon SP,Hendil K,Tanaka K,Fishel S,Mayer RJdoi
10.1093/molehr/3.12.1053subject
Has Abstractpub_date
1997-12-01 00:00:00pages
1053-60issue
12eissn
1360-9947issn
1460-2407journal_volume
3pub_type
杂志文章abstract::We have developed a competitive reverse transcription-polymerase chain reaction (RT-PCR) sensitive enough to detect and quantify as little as 2-fold differences in gene expression in individual oocytes and embryos throughout human preimplantation development. This RT-PCR assay can be tailored for the examination of an...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/7.2.147
更新日期:2001-02-01 00:00:00
abstract:STUDY QUESTION:Is it possible to identify original compounds that are able to enhance sperm motility from the venom of the scorpion Scorpio maurus palmatus? SUMMARY ANSWER:We identified a potent disulfide-rich peptide (DRP) of 73 amino acids that significantly improved the motility of fresh and frozen-thawed sperm in ...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaw075
更新日期:2017-02-10 00:00:00
abstract::Prevention of mitochondrial DNA (mtDNA) diseases may currently be possible using germline nuclear transfer (NT). However, scientific evidence to compare efficiency of different NT techniques to overcome mtDNA diseases is lacking. Here, we performed four types of NT, including first or second polar body transfer (PB1/2...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaz062
更新日期:2019-12-01 00:00:00
abstract::Oligozoospermia (low sperm count) is a common semen deficiency. However, to date, few genetic defects have been identified to cause this condition. Moreover, even fewer molecular genetic diagnostic tests are available for patients with oligozoospermia in the andrology clinic. Based on animal and gene expression studie...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gat008
更新日期:2013-06-01 00:00:00
abstract::SUN domain proteins are integral proteins of the inner nuclear membrane and functions in linkage of the nuclear lamina to the cytoskeleton. Moreover, SUN domain proteins seem to mediate the tethering of the centrosome to the nuclear membrane, and they are involved in telomere attachment to the nuclear envelope in meio...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaq099
更新日期:2011-04-01 00:00:00
abstract::The expression of insulin-like growth factor-I (IGF-I) was measured at the mRNA and protein level in myometrium and fibroids from women with and without preoperative treatment with a gonadotrophin-releasing hormone (GnRH) agonist for 3 months, from post-menopausal women, from pregnant women and in myometrium from wome...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/6.10.915
更新日期:2000-10-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/2.1.2
更新日期:1996-01-01 00:00:00
abstract:STUDY HYPOTHESIS:In women with preterm premature rupture of the membranes (PPROM), increased oxidative stress may accelerate premature cellular senescence, senescence-associated inflammation and proteolysis, which may predispose them to rupture. STUDY FINDING:We demonstrate mechanistic differences between preterm birt...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gav074
更新日期:2016-02-01 00:00:00
abstract:STUDY QUESTION:Can enzymatically dispersed testicular cells from adult men reassemble into seminiferous cord-like structures in vitro? SUMMARY ANSWER:Adult human testicular somatic cells reassembled into testicular cord-like structures via dynamic interactions of Sertoli and peritubular cells. WHAT IS KNOWN ALREADY:I...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gax063
更新日期:2018-02-01 00:00:00
abstract:STUDY QUESTION:Does A20 regulate mediators involved in the terminal processes of human labour in primary myometrial and amnion cells? SUMMARY ANSWER:A20 is a nuclear factor-kappa B (NF-κB) responsive gene that acts as a negative regulator of NF-κB-induced expression of pro-labour mediators. WHAT IS KNOWN ALREADY:Infl...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gax041
更新日期:2017-09-01 00:00:00
abstract::Development of uterine glands (adenogenesis) in mammals typically begins during the early post-natal period and involves budding of nascent glands from the luminal epithelium and extensive cell proliferation in these structures as they grow into the surrounding stroma, elongate and mature. Uterine glands are essential...
journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
doi:10.1093/molehr/gat031
更新日期:2013-09-01 00:00:00
abstract::Folate deficiency has been linked to a wide range of pregnancy disorders. Most research about folate-deficiency has focused on the embryo itself, little attention has been paid to possible effects on the placenta. According to our results, the morphology of the placenta, endocrine function, and the expression of genes...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaz022
更新日期:2019-06-06 00:00:00
abstract::The endocrine disrupting chemical o, p'-dichlorodiphenyltrichloroethane (DDT) can affect reproductive organs, tissues and cells in several species. Treatment of human endometrial endothelial cells (HEECs) with 50 microM o,p'-DDT decreased their proliferation compared with the control. Microarray analyses revealed that...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gam091
更新日期:2008-02-01 00:00:00
abstract::A novel gene, TSG23/Tsg23, was identified by comparing the expression profiles of human adult and fetal testis using Affymetrix Genechips. RT-PCR analysis from multiple human and mouse tissues indicated TSG23/Tsg23 mRNA was mainly expressed in the testis. In situ hybridization revealed that TSG23/Tsg23 mRNA was locate...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gap015
更新日期:2009-04-01 00:00:00
abstract::MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression post-transcriptionally. We explored whether connexin 43 (Cx43) was differently expressed in luteinized granulosa cells from women with polycystic ovary syndrome (PCOS) compared with luteinized granulosa cells from women with a normal...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaaa044
更新日期:2020-08-01 00:00:00
abstract::Round spermatids contain high levels of extremely varied mRNAs that are synthesized either throughout early spermatogenesis or during spermiogenesis from the haploid genome. Concomitantly, with major changes in the chromatin organization, arrest of transcription occurs at midspermiogenesis. However, previous investiga...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah137
更新日期:2005-02-01 00:00:00
abstract::Preimplantation development shifts from a maternal to embryonic programme rapidly after fertilization. Although the majority of oogenetic products are lost during the maternal to embryonic transition (MET), several do survive this interval to contribute directly to supporting preimplantation development. Embryonic gen...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gan063
更新日期:2008-12-01 00:00:00
abstract::Human pregnancy represents a situation of semiallograft to maternal host. Therefore, it has been reported that tolerance to the fetal allograft represents a mechanism for maintaining a pregnancy. CD4(+)CD25(bright) regulatory T cells are known to play an important role in the development and maintenance of tolerance i...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah044
更新日期:2004-05-01 00:00:00
abstract::Oocyte quality is one of the most important aspects of in vitro embryo development. Extensive epigenetic programming must occur during oocyte growth and maturation. A specific DNA methylation pattern of the imprinted genes must be established on differentially methylated regions (DMR). The insulin-like growth factor 2...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaq075
更新日期:2011-02-01 00:00:00
abstract::Retrograde flow of menstrual blood cells during menstruation is considered as the dominant theory for the development of endometriosis. Moreover, current evidence suggests that endometrial-derived stem cells are key players in the pathogenesis of endometriosis. In particular, endometrial stromal stem cells have been s...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau044
更新日期:2014-09-01 00:00:00
abstract::An understanding of the relationship between nuclear morphology and DNA function is important in cytology and preimplantation diagnosis. In this study, direct polymerase chain reaction (PCR) amplification was used to diagnose the common delta F508 mutation of cystic fibrosis in 62 biopsied human embryo cells. The nucl...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/2.1.63
更新日期:1996-01-01 00:00:00
abstract:STUDY QUESTION:How does hCG signal in human endometrial stromal cells (ESCs) and what is its role in regulating ESC function? SUMMARY ANSWER:hCG signaling in ESCs activates the extracellular signal-regulated protein kinases 1 and 2 (Erk1/2) pathway through exchange protein activated by cyclic AMP (cAMP) (Epac) and tra...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gax015
更新日期:2017-06-01 00:00:00
abstract::In mature mammalian oocytes, cortical f-actin distribution is polarized, as evidenced by a prominent cap subtended by the metaphase II (MII) spindle. Formation of a polarized actin cap is a consequence of a complex actomyosin-driven contractile process that directs polar body extrusion. Human mature oocytes also displ...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gat085
更新日期:2014-03-01 00:00:00
abstract::Natural killer (NK) cells are the predominant leukocyte in first trimester decidua and play a role in vascular remodelling through interferon gamma (IFNgamma) secretion. Membrane expression of the killer immunoglobulin-like receptor (KIR) KIR2DL4 on peripheral blood NK (pNK) cells is controlled by the 9A/10A transmemb...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gap039
更新日期:2009-08-01 00:00:00
abstract::A molecule isolated from the peritoneal fluids of women undergoing laparoscopy for in-vitro fertilization techniques has been chemically characterized and identified as 1-palmitic-3-phosphorylcholine (lysophosphatidylcholine, LPC). This lipid is able, at physiological concentrations, to completely inhibit sperm motili...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/3.3.241
更新日期:1997-03-01 00:00:00
abstract::The clinical application of quantitative fluorescent polymerase chain reaction (QF-PCR) for rapid prenatal detection of chromosome aneuploidies has been limited in most studies to the detection of autosomal trisomies. Recently it has been shown that a newly identified highly polymorphic marker, termed X22, which maps ...
journal_title:Molecular human reproduction
pub_type: 临床试验,杂志文章
doi:10.1093/molehr/7.10.1001
更新日期:2001-10-01 00:00:00
abstract::We report here the molecular cloning and characterization of human haspin cDNA and its genomic DNA construct. The haspin protein is a unique protein kinase, first isolated from mouse testis. Specifically expressed in mouse testicular germ cells, haspin is suggested to play a role in cell cycle arrest in haploid sperma...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/7.3.211
更新日期:2001-03-01 00:00:00
abstract::Morphological assessments are the main way in which fertility clinics select in vitro generated embryo(s) for transfer to the uterus. However, it is widely acknowledged that the microscopic appearance of an embryo is only weakly correlated with its viability. Furthermore, the extent to which morphology is affected by ...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gat073
更新日期:2014-02-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaz010
更新日期:2019-04-01 00:00:00
abstract::Prader-Willi syndrome (PWS) is a genomic disorder mostly caused by deletions of 15q11-q13 region (70%). It has been suggested that the particular genomic architecture of 15q11-q13 region, characterized to be flanked by low copy repeats, could predispose it to Non-Allelic Homologous Recombination (NAHR). However, no st...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaq005
更新日期:2010-05-01 00:00:00