Identification of transcripts by macroarrays, RT-PCR and in situ hybridization in human ejaculate spermatozoa.

abstract：STUDY QUESTION:Is oxidative stress associated with the A disintegrin and metalloproteases (ADAM) metallopeptidase domain 17 (ADAM17)/Notch signalling pathway and fibrosis in the development of endometriosis? SUMMARY ANSWER:Oxidative stress is correlated with hyperactivation of the ADAM17/Notch signalling pathway and a...

journal_title：Molecular human reproduction

authors： González-Foruria I,Santulli P,Chouzenoux S,Carmona F,Chapron C,Batteux F

更新日期：2017-07-01 00:00:00

abstract：:In early gestation, the human placental renin-angiotensin system (RAS) is upregulated and plays a role in placental development. Among other functions, signalling through the angiotensin II type 1 receptor (AT1R) initiates proliferation. Many microRNAs (miRNAs) targeting placental RAS mRNAs are downregulated at this t...

journal_title：Molecular human reproduction

authors： Arthurs AL,Lumbers ER,Pringle KG

更新日期：2019-04-01 00:00:00

abstract：:Before the advent of intracytoplasmic sperm injection (ICSI) semen preparation techniques focused on the need to sustain the fertilizing potential of the spermatozoa particularly by reducing oxidative stress. However, for severely oligozoospermic patients treated by ICSI, sperm preparation protocols are used which aim...

journal_title：Molecular human reproduction

authors： Twigg J,Irvine DS,Houston P,Fulton N,Michael L,Aitken RJ

更新日期：1998-05-01 00:00:00

abstract：:Prader-Willi syndrome (PWS) is a genomic disorder mostly caused by deletions of 15q11-q13 region (70%). It has been suggested that the particular genomic architecture of 15q11-q13 region, characterized to be flanked by low copy repeats, could predispose it to Non-Allelic Homologous Recombination (NAHR). However, no st...

journal_title：Molecular human reproduction

authors： Molina O,Blanco J,Vidal F

更新日期：2010-05-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is relevant for normal pregnancy, and abnormalities in VEGF functions are associated with hypertensive disorders of pregnancy. Because there are few studies on how VEGF genetic polymorphisms affect susceptibility to pre-eclampsia (PE), and no studies on how they affect suscept...

journal_title：Molecular human reproduction

authors： Sandrim VC,Palei AC,Cavalli RC,Araújo FM,Ramos ES,Duarte G,Tanus-Santos JE

更新日期：2009-02-01 00:00:00

abstract：:Preimplantation development shifts from a maternal to embryonic programme rapidly after fertilization. Although the majority of oogenetic products are lost during the maternal to embryonic transition (MET), several do survive this interval to contribute directly to supporting preimplantation development. Embryonic gen...

journal_title：Molecular human reproduction

authors： Bell CE,Calder MD,Watson AJ

更新日期：2008-12-01 00:00:00

abstract：:The molecular mechanism of stress-associated reproductive dysfunction is complex and largely unknown. This study was designed to systematically analyze molecular effects of systemic in vivo blockade of α1-adrenergic receptors (α1-ADRs) on stress-induced disturbance of cAMP/cGMP signaling in testosterone-producing Leyd...

journal_title：Molecular human reproduction

authors： Stojkov NJ,Baburski AZ,Bjelic MM,Sokanovic SJ,Mihajlovic AI,Drljaca DM,Janjic MM,Kostic TS,Andric SA

更新日期：2014-01-01 00:00:00

abstract：:Interleukin (IL)-33, a newly described member of the IL-1 family, has been reported to facilitate primary tumor progression and metastatic dissemination. However, its biological function on decidual stromal cells (DSCs) remains unclear. In this study, we tested the hypothesis whether IL-33 promotes proliferation and i...

journal_title：Molecular human reproduction

authors： Hu WT,Li MQ,Liu W,Jin LP,Li DJ,Zhu XY

更新日期：2014-04-01 00:00:00

abstract：:Inhibin production has been demonstrated in malignant epithelial ovarian tumours, but secretion of inhibins by benign cystadenoma has not yet been reported. The present study evaluated the concentrations of inhibin A and inhibin B and the relationship with oestradiol and nitric oxide metabolites in fluid collected fro...

journal_title：Molecular human reproduction

authors： Reis FM,Faletti A,Luisi S,Bifulco G,Cauci S,Quadrifoglio F,Dor J,Petraglia F

更新日期：2000-12-01 00:00:00

abstract：:Previous studies have implicated receptor tyrosine kinases in progesterone-induced [Ca2+]i signalling, and consequent induction of the acrosome reaction, in human spermatozoa. We have investigated the effects of tyrosine kinase inhibition on [Ca2+]i responses in large numbers of individual human spermatozoa. Genistein...

journal_title：Molecular human reproduction

authors： Kirkman-Brown JC,Lefièvre L,Bray C,Stewart PM,Barratt CL,Publicover SJ

更新日期：2002-04-01 00:00:00

abstract：:Premature loss of ovarian activity before 40 years of age is known as primary ovarian insufficiency (POI) and occurs in ∼1% of women. A more subtle decline in ovarian activity, known as premature ovarian ageing (POA), occurs in ∼10% of women. Despite the high prevalence of POA, very little is known regarding its genet...

journal_title：Molecular human reproduction

authors： Subramanian GN,Lavin M,Homer HA

更新日期：2020-12-18 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is a heterogeneous condition with unknown etiology and is considered to be the most common endocrine disorder in women of reproductive age. Two meta-analyses are presented here concerning the association of Plasminogen Activator Inhibitor 1 (PAI-1) 4G/5G polymorphism and the methylene-...

journal_title：Molecular human reproduction

authors： Bagos PG

更新日期：2009-01-01 00:00:00

abstract：:Epidemiological and experimental animal studies show that suboptimal environments in fetal and neonatal life exert a profound influence on physiological function and risk of diseases in adult life. The concepts of the 'developmental programming' and Developmental Origins of Health and Diseases (DOHaD) have become well...

journal_title：Molecular human reproduction

authors： Chen T,Liu HX,Yan HY,Wu DM,Ping J

更新日期：2016-08-01 00:00:00

abstract：:The endocrine disrupting chemical o, p'-dichlorodiphenyltrichloroethane (DDT) can affect reproductive organs, tissues and cells in several species. Treatment of human endometrial endothelial cells (HEECs) with 50 microM o,p'-DDT decreased their proliferation compared with the control. Microarray analyses revealed that...

journal_title：Molecular human reproduction

authors： Bredhult C,Sahlin L,Olovsson M

更新日期：2008-02-01 00:00:00

abstract：STUDY HYPOTHESIS:It is possible to isolate pure populations of single potential human spermatogonial stem cells without somatic contamination for down-stream applications, for example cell culture and gene expression analysis. STUDY FINDING:We isolated pure populations of single potential human spermatogonial stem cel...

journal_title：Molecular human reproduction

authors： von Kopylow K,Schulze W,Salzbrunn A,Spiess AN

更新日期：2016-04-01 00:00:00

abstract：:In the implantation, trophoblasts penetrate maternal decidua by secreting proteases. It has been reported that cathepsins are highly expressed in the mouse villi, and play an important role in normal embryonal growth and decidualization. In this study, we evaluated cathepsins and their endogenous inhibitors, cystatins...

journal_title：Molecular human reproduction

authors： Nakanishi T,Ozaki Y,Blomgren K,Tateyama H,Sugiura-Ogasawara M,Suzumori K

更新日期：2005-05-01 00:00:00

abstract：:Successful embryo implantation depends on the quality of the embryo, as well as on the receptivity of the endometrium. The aim of this study was to investigate the endometrial gene expression profile in women with unexplained infertility in comparison with fertile controls at the time of embryo implantation in order t...

journal_title：Molecular human reproduction

authors： Altmäe S,Martínez-Conejero JA,Salumets A,Simón C,Horcajadas JA,Stavreus-Evers A

更新日期：2010-03-01 00:00:00

abstract：:Surfactant protein D (SP-D) plays a role in innate immunity against various pathogens and in vivo studies have demonstrated that SP-D also has anti-inflammatory properties. SP-D was originally demonstrated in alveolar type II cells, but recent studies have shown extrapulmonary expression of SP-D indicating a systemic ...

journal_title：Molecular human reproduction

authors： Leth-Larsen R,Floridon C,Nielsen O,Holmskov U

更新日期：2004-03-01 00:00:00

abstract：:Stromal cell-derived factor-1 (SDF-1 or CXCL12) is the physiologic ligand for the chemokine receptor CXCR4. CXCR4-mediated signalling regulates cell migration and apoptosis in certain haematopoietic and neuronal cells. Using gene profiling, we determined that CXCR4 is the only chemokine receptor for which mRNA express...

journal_title：Molecular human reproduction

authors： Jaleel MA,Tsai AC,Sarkar S,Freedman PV,Rubin LP

更新日期：2004-12-01 00:00:00

abstract：:This study was undertaken to evaluate the effects of gamma-aminobutyric acid (GABA) and GABAergic agonists and antagonists on sperm kinematic parameters and hyperactivation, evaluated by a computer-assisted semen analysis (CASA) system, and intracellular cAMP content in 22 normozoospermic semen samples. Because of the...

journal_title：Molecular human reproduction

authors： Calogero AE,Hall J,Fishel S,Green S,Hunter A,D'Agata R

更新日期：1996-10-01 00:00:00

abstract：:Matrix metalloproteinases (MMP), tissue inhibitors of metalloproteinases (TIMP), and MMP-TIMP interactions may contribute to the highly programmed process of embryo implantation. The loss of the delicate MMP-TIMP balance may lead to abnormal implantation. The role of TIMP-4 in mouse implantation has not been reported....

journal_title：Molecular human reproduction

authors： Zhang J,Zhao YG,Cao YJ,Sang QX,Duan EK

更新日期：2003-03-01 00:00:00

abstract：:Preeclampsia (PE) is a severe pregnancy-induced disorder characterized by hypertension and proteinuria and a leading cause of perinatal maternal-fetal mortality and morbidity in developing countries. Dysregulated human leukocyte antigen (HLA)-G was found in placentas as well as in maternal sera from PE patients; howev...

journal_title：Molecular human reproduction

authors： Tang Y,Liu H,Li H,Peng T,Gu W,Li X

更新日期：2015-09-01 00:00:00

abstract：:We report an improvement in the PGD test for fragile X syndrome (FXS). Recently, multiple displacement amplification (MDA) has been reported to yield large amounts of DNA from single cells. Taking into account this technique, we developed a new PGD test for FXS, enabling combined analysis of linked polymorphic markers...

journal_title：Molecular human reproduction

authors： Burlet P,Frydman N,Gigarel N,Kerbrat V,Tachdjian G,Feyereisen E,Bonnefont JP,Frydman R,Munnich A,Steffann J

更新日期：2006-10-01 00:00:00

abstract：:Ovarian hyperstimulation syndrome (OHSS) is a potentially life-threatening, iatrogenic complication of ovarian stimulation in assisted reproduction technology. This complex syndrome is characterised by enlarged ovaries with multiple corpora luteum, elevated sex steroid hormones in serum and increased capillary permeab...

journal_title：Molecular human reproduction

authors： Liu W,Zhang C,Wang L,Huang X,Zhang J,He Y,Chen L,Li J

更新日期：2019-08-01 00:00:00

abstract：STUDY QUESTION:Can RNA sequencing of human cumulus cells (CC) reveal molecular pathways involved in the physiology of reproductive aging? STUDY FINDING:Senescent but not young CC activate gene pathways associated with hypoxia and oxidative stress. WHAT IS KNOWN ALREADY:Shifts in socioeconomic norms are resulting in l...

journal_title：Molecular human reproduction

authors： Molinari E,Bar H,Pyle AM,Patrizio P

更新日期：2016-08-01 00:00:00

abstract：:Follicular fluid proteoglycans play an important role in human oocyte maturation, including the development of a fluid-filled compartment and maintenance of the hypocoagulative state of the follicular fluid. Human xylosyltransferase (EC 2.4.2.26, XT) is the key enzyme in the biosynthesis of glycosaminoglycan chains in...

journal_title：Molecular human reproduction

authors： Götting C,Kuhn J,Tinneberg HR,Brinkmann T,Kleesiek K

更新日期：2002-12-01 00:00:00

abstract：:We report here the molecular cloning and characterization of human haspin cDNA and its genomic DNA construct. The haspin protein is a unique protein kinase, first isolated from mouse testis. Specifically expressed in mouse testicular germ cells, haspin is suggested to play a role in cell cycle arrest in haploid sperma...

journal_title：Molecular human reproduction

authors： Tanaka H,Iguchi N,Nakamura Y,Kohroki J,de Carvalho CE,Nishimune Y

更新日期：2001-03-01 00:00:00

abstract：:Granzyme B (graB) plays a pivotal role in cytotoxic lymphocyte granule-mediated apoptosis through cleavage of intracellular proteins in target cells. Proteinase inhibitor-9 (PI-9) is a potent inhibitor of graB and is highly expressed in cytotoxic lymphocytes. Here, we show by immunohistochemistry that PI-9 is also abu...

journal_title：Molecular human reproduction

authors： Hirst CE,Buzza MS,Sutton VR,Trapani JA,Loveland KL,Bird PI

更新日期：2001-12-01 00:00:00

abstract：:The reproductive lifespan of a woman is determined by the gradual recruitment of quiescent follicles into the growing pool. In humans, ovarian tissue removal from its in vivo environment induces spontaneous activation of resting follicles. Similarly, pharmacological activation of the PI3K/Akt pathway leads to accelera...

journal_title：Molecular human reproduction

authors： Grosbois J,Vermeersch M,Devos M,Clarke HJ,Demeestere I

更新日期：2019-11-30 00:00:00

abstract：:A major challenge in the comprehension of the endometrial transformations leading to the completion of each menstrual cycle in humans is in the identification of specific molecular pathways underlying these monthly turnovers. Towards this goal we compared, by the differential display technique, the relative expression...

journal_title：Molecular human reproduction

authors： Malette B,Cherry E,Lagacé M,Bernard M,Gosselin D,Hugo P,Shazand K

更新日期：2003-11-01 00:00:00