Abstract：
:Biocytin, a naturally occurring low molecular weight analog of biotin, was evaluated as a neuroanatomical tract-tracing marker in the adult rat brain. Since it retains high-affinity binding to avidin, biocytin can be labelled with avidinylated visualization reagents. Iontophoretic or pressure injections resulted in filling of cell bodies and dendrites around the injection site and their efferent axonal processes and boutons. Retrogradely labelled neurons were occasionally observed at a distance but only with large injections. Anterograde tracing with biocytin is successful even in animals that are quite old, in contrast to lectins and HRP conjugates, and offers advantages in delivery, tissue processing, selection of light and/or electron microscopic labels, time to obtain results, and cost over many conventional tracers.
journal_name
Brain Resjournal_title
Brain researchauthors
King MA,Louis PM,Hunter BE,Walker DWdoi
10.1016/0006-8993(89)90281-3subject
Has Abstractpub_date
1989-09-18 00:00:00pages
361-7issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(89)90281-3journal_volume
497pub_type
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