Abstract:
:The binding of fluorescein and 6-carboxyfluorescein by normal, diabetic and in vitro glycosylated human serum proteins was analyzed by absorption and fluorescence spectroscopy, gel filtration and equilibrium dialysis. The absorption spectra of bound fluorochromes showed red shifts and hypochromic changes, and fluorochrome fluorescence was reduced by serum proteins. Bio-gel P-6 and Sephadex G-200 gel filtration in solvents containing free fluorochrome demonstrated that fluorescein and carboxyfluorescein were bound by serum proteins of varying molecular weights and/or diffusion rates. Equilibrium dialysis at 37 degrees C showed that human sera contained 3.5 +/- (SD) 0.4 X 10(-3)M of fluorescein and carboxyfluorescein binding sites with an average association constant of 3.9 +/- 0.2 X 10(-3)M-1. Undiluted serum proteins bound 93% of the fluorochrome at a total concentration of 2 X 10(-4)M or less. Glycosylation of serum proteins in vivo or in vitro did not change the concentration-dependent or pH-dependent binding of fluorescein or carboxyfluorescein.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Rockey JH,Li Wdoi
10.1159/000265219subject
Has Abstractpub_date
1982-01-01 00:00:00pages
416-27issue
6eissn
0030-3747issn
1423-0259journal_volume
14pub_type
杂志文章abstract:AIM/BACKGROUND:A 1-bp deletion (1147delA) in the SAG (also known as arrestin or S-antigen) gene is the most frequently seen mutation in Japanese patients suffering from Oguchi disease, a recessively inherited stationary night blindness. We investigated macular function in a patient with Oguchi disease with the 1147delA...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000325024
更新日期:2011-10-01 00:00:00
abstract::In corneal organ culture, a contamination of sterile culture media with endotoxin is frequently found. Thus, we investigated if the presence of endotoxin affects the viability of cultured porcine corneal endothelial cells. Endotoxin in high concentrations caused morphological cell changes in porcine corneal endothelia...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055566
更新日期:1999-01-01 00:00:00
abstract:PURPOSE:The possibility of providing cultured corneal endothelial cells (CECs) for clinical transplantation has gained much attention. However, the worldwide need for human (h) donor corneas far exceeds supply. The pig (p) might provide an alternative source. The aim of this study was to compare the proliferative capac...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000342978
更新日期:2013-01-01 00:00:00
abstract::Cellular immune responses in retinal autoimmunity have scarcely been studied in rabbits. An effective isolation of rabbit peripheral blood lymphocytes is difficult to achieve and requires specific conditions. This hampers especially the assay of antigen-induced lymphocyte transformation. We have improved the lymphocyt...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000266934
更新日期:1989-01-01 00:00:00
abstract::The ultrastructure of optic nerve axons was examined in several mammals (human, cat, rat, sheep, ox, pig, guinea pig, rabbit). Human material was obtained from normotensive, glaucoma-free eyes and from eyes with a history of glaucoma and raised intra-ocular pressure (IOP). We describe accumulations of organelles (prin...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267739
更新日期:1995-01-01 00:00:00
abstract:INTRODUCTION:Diabetic Retinopathy (DR) is one of the major vision-threatening causes worldwide. Searching for an individualized therapeutic strategy to prevent its progress is challenging. OBJECTIVE:This work aimed to investigate the association of angiogenesis-inducer vascular endothelial growth factor (VEGF) gene fa...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000511087
更新日期:2020-08-24 00:00:00
abstract::To investigate the binding of peanut-agglutinin (PNA) on porcine corneal tissue, several methods of tissue processing were used. In cryostat sections of postmortem tissue, PNA labeling of epithelial cell membranes was detected. In the stroma, a fibrillar structure parallel to the corneal surface was found. The anterio...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000266748
更新日期:1988-01-01 00:00:00
abstract::Chloroquine retinopathy was produced experimentally in the eye of the albino corydoras (one of the tropical fish) by daily administration of chloroquine (0.1 mg per os). The enucleated eyes were examined from the 14th day to 3 months after the beginning of drug administration under light and electron microscopy. The f...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265429
更新日期:1986-01-01 00:00:00
abstract::Autofluorescence and transmission values of the lens for blue-green light were determined at two locations with the use of identical fluorophotometers: in Coimbra, Portugal, in 44 healthy controls, 15 haemodialysis and 11 renal failure patients, and in Leiden, The Netherlands, in 57 healthy controls, 23 haemodialysis ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000266736
更新日期:1988-01-01 00:00:00
abstract::Substance P (SP) immunoreactivity was demonstrated using the indirect immunofluorescence technique in one normal and one retinoblastomatous human retina. In the normal retina SP immunoreaction was located in nerve fibres but not in the neurons in the inner plexiform layer. A similar location was observed in the histol...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265276
更新日期:1983-01-01 00:00:00
abstract::Dry eye is the most prominent pathology among those involving the ocular surface: a decrease of the aqueous (less frequent) or the lipid (more frequent) component of the tear film is the cause of the diminished stability of tears that is observed in this pathology. Dry eye shows a clear distribution linked to both sex...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000489093
更新日期:2018-01-01 00:00:00
abstract::The purpose of this study was to compare the variability of the amplitude ratios and latencies of the electro-oculogram (EOG) light peak (LP) in three different recording conditions: Arden ratio (dark trough (DT): 12 min), modified Arden ratio (DT: 15 min) and light peak/dark baseline ratio (LP/BL: 35 min of dark adap...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267320
更新日期:1993-01-01 00:00:00
abstract::Correlation proton nuclear magnetic resonance spectroscopy was performed on light- and dark-adapted rabbit retinas to elucidate real-time hexose monophosphate shunt (HMPS) activity. Light significantly stimulated retinal HMPS initially, which consumed 17% more glucose than that in dark-adapted retinas. The glucose con...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265511
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND/AIMS:To evaluate the safety of suramin compared with mitomycin-C (MMC) as an adjunctive agent in trabeculectomy by determining its ciliary body toxicity at predetermined effective dosages in rabbit eyes. METHODS:Thirty-two New Zealand albino rabbits received either suramin (200, 300, 400, or 800 mg/ml) or M...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000187625
更新日期:2009-01-01 00:00:00
abstract::The pathology of rainbow trout lenses incubated in various media and at various temperatures was examined by electron microscopy. After incubation at 0 degrees C for 5 h, no changes were observed in transparency and cytology. Irreversible warm cataract occurred at 37 degrees C, which corresponds to the body temperatur...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265427
更新日期:1986-01-01 00:00:00
abstract::Inflammation is substantially contributing to the development and worsening of diabetic retinopathy in general and diabetic macular edema (DME) in particular, which provides the rationale to treat DME with corticosteroids. While anti-vascular endothelial growth factor (VEGF) agents are mostly chosen as a first-line tr...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000499540
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:The aim of this work was to evaluate the narrow anterior chamber angle (ACA) in Chinese patients by anterior segment optical coherence tomography (As-OCT). METHODS:Eighty right eyes of 80 consecutive patients with a shallow peripheral anterior chamber but no peripheral anterior synechia were enrolled in thi...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000448523
更新日期:2017-01-01 00:00:00
abstract::The purpose of this study was to determine the expression of metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in the aqueous humour of patients with exfoliation syndrome (XFS). XFS and control samples were analysed for their MMP content by zymography and for their tissue inhibitors by ELISA. In XFS eyes, ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000063661
更新日期:2002-05-01 00:00:00
abstract:INTRODUCTION:Retinitis pigmentosa (RP) is an inherited retinal disorder, characterized by photoreceptor degeneration inducing progressive vision loss. This study evaluates its impact on quality of life (QOL) and emotional states of patients affected by RP. METHODS:A cross-sectional study was conducted on 60 RP patient...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000435886
更新日期:2015-01-01 00:00:00
abstract::Two out of three normal eyes tested with frequent instillation of 30 microliters of drops of 0.5% bupranolol, a beta 1 + beta 2 blocking drug with some intrinsic sympathomimetic and local anesthetic activity, showed a miotic effect of the order of a 25% reduction of pupil area, as estimated by an open-loop iris-videor...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265407
更新日期:1986-01-01 00:00:00
abstract:PURPOSE:To investigate therapeutic ocular surface medium (TOSM), a potential physiological tear replacement therapy, for persistent epithelial defect (PED). METHODS:11 eyes of 10 patients with PED for ≥ 2 weeks without improvement despite conventional treatment were enrolled in a prospective pilot study of TOSM over 1...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000355065
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the sensitivity and specificity of pseudoexfoliation syndrome diagnosis in pseudophakic patients and potential means of improving it. METHODS:This prospective, nonrandomized study comprised 41 consecutive patients (41 eyes) scheduled for cataract surgery at a tertiary medical center during 2016-2...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000508336
更新日期:2020-04-30 00:00:00
abstract:BACKGROUND:The aim of this study is to provide a quantitative evaluation of the blood-aqueous barrier (BAB) in patients with ocular blunt trauma and evaluate its association with intraocular pressure (IOP) elevation. METHODS:This is a prospective case-control study, and the following 3 groups were included: elevated I...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000445352
更新日期:2016-07-01 00:00:00
abstract::Topcon Tokyo manufactured the first series of SL-45 in 1980 and a second in 1982/83, which were completed in Bonn with all technical improvements developed so far. In 1987, the SL-45B was manufactured which included all previously added improvements. To compare both camera types, we carried out a test with 10 voluntee...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267057
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:Osthole is an agent isolated from Cnidium monnieri (L.) Cusson and has been used to treat several disorders. Corneal neovascularization is a sight-threatening condition associated with several inflammatory or infectious ocular disorders. In this study, we investigated the anti-angiogenic effects of osthole on c...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000505976
更新日期:2020-01-01 00:00:00
abstract:PURPOSE:Abnormalities in electroretinograms (ERG), such as reduced amplitudes and delayed implicit times of a- and b-wave and oscillatory potentials (OPs), are one of the earliest features of diabetic retinopathy prior to obvious vascular changes in diabetic retinas. We have previously shown that serum levels of advanc...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000440768
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:Single-field non-mydriatic fundus photography (NMFP) has been used to detect diabetic retinopathy (DR) in many studies; however, its value in a general clinical setting has not been established. Here we performed a meta-analysis to evaluate its diagnostic effectiveness. METHOD:We systematically searched PubMed...
journal_title:Ophthalmic research
pub_type: meta分析
doi:10.1159/000499106
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:To propose a new noncontact tonometer based on corneal photoelasticity. METHOD:In this study, we experimented with 18 enucleated porcine eyes. The anterior chamber was infused with a physiological solution. A circular polarizing filter was attached to an ophthalmic surgical microscope. Color fringe changes at ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000320600
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:This study was aimed at determining the intraocular pharmacokinetics based on molecular physicochemical properties in a rabbit model. METHODS:The entire dataset was obtained from previous literature, and research articles regarding 70 molecular compounds were investigated. The intravitreal half-lives in rabbit...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000499529
更新日期:2020-01-01 00:00:00
abstract::Non-toxic concentrations of ceftazidime (CAZ), cefuzonam sodium (CZON) and cefmetazole sodium (CMZ) for intravitreal use were assessed by the electroretinogram in in vitro perfused eyecups of albino rabbits. None of the a-wave, the b-wave and the oscillatory potentials deteriorated with 300 microM CAZ, 300 microM CZON...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267858
更新日期:1995-01-01 00:00:00