Abstract：
:The effect of the tricarboxylic acid (TCA) cycle precursor, pyruvate, on glutamine metabolism by isolated renal cortical mitochondria was assessed by quantitating its key nitrogen and carbon metabolites. When mitochondria from normal rats were incubated at pH 7.4, pyruvate (2 mM) inhibited ammonia production by almost completely erradicating glutamate deamination and by diminishing glutamine deamidation but to a lesser extent. Alpha KG, citrate, and malate accumulation in the incubation medium were increased dramatically reflecting the increased flux of pyruvate through the TCA cycle; the intramitochondrial concentrations of both Alpha KG and glutamate were increased. Thus, pyruvate primarily inhibits flux through glutamate dehydrogenase as a result either of an increase in Alpha KG concentration and/or a decrease in the redox (NAD/NADH) potential secondary to enhanced flux through the TCA cycle. Glutamine deamidation is secondarily inhibited, presumably due to the increased intramitochondrial concentration of glutamate. Citrate (2 mM) produced changes comparable to those observed with pyruvate. Mitochondria from normal rats incubated at pH 7.0 as well as mitochondria from rats with chronic metabolic acidosis responded to pyruvate in a fashion qualitatively similar to normal mitochondria incubated at pH 7.4. Glutamate deamination was inhibited significantly, but a high rate persisted with chronic acidosis despite the presence of pyruvate. Nevertheless, when glutamine metabolism was contrasted with normal mitochondria incubated at pH 7.4, the response to in vitro incubation in an acid pH as well as to chronic metabolic acidosis was similar quantitatively regardless of whether glutamine alone or in combination with pyruvate was present in the incubation medium.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Tannen RL,Kunin ASdoi
10.1038/ki.1982.166subject
Has Abstractpub_date
1982-09-01 00:00:00pages
280-5issue
3eissn
0085-2538issn
1523-1755pii
S0085-2538(15)32801-5journal_volume
22pub_type
杂志文章abstract：:We sought to study glomerular pathophysiology in a model of IgA nephropathy (IgAN). Preliminary experiments with oral immunization indicated that Lewis rats had higher IgA levels and IgA/IgG ratios than Wistar, Fischer or Sprague-Dawley rats. Six groups of Lewis rats were studied: four continuously orally immunized fo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.268
更新日期：1992-07-01 00:00:00
abstract：BACKGROUND:Familial nephrotic syndrome (NS) has both autosomal dominant and recessive forms of inheritance. Recent studies in families with an autosomal dominant form of focal segmental glomerulosclerosis (FSGS) have been at odds concerning linkage to chromosome 19q13 (Mathis et al, Kidney Int 53:282-286, 1998; Winn et...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.057003875.x
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:Vascular endothelial growth factor (VEGF) and its receptors have been implicated in the pathogenesis of diabetic nephropathy. The objective of this study was to determine whether alterations of the plasma and urinary VEGF and sFLT-1 levels were related to the stages and risk factors of diabetic nephropathy. ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00067.x
更新日期：2005-01-01 00:00:00
abstract：:We identified a novel mutation in the COL4A5 gene of a Japanese patient with Alport syndrome. A combination of in vitro amplification of the exons with single strand conformation polymorphisms (SSCP) analysis suggested the presence of a mutation in exon 48. Sequencing of the amplified DNA revealed a single base (T) in...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1993.353
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND:The Banff working formulation of renal transplant pathology is intended to have international application. There remains a need to develop methods to harmonize the application of such grading systems between laboratories. Banff grades do not always permit precise management decisions to be made. Alternative ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00030.x
更新日期：2001-11-01 00:00:00
abstract：:The candidacidal activity of kidneys, liver, and spleen's phagocytic systems was studied in mouse. Different strains of mice were inoculated intravenously (i.v.) with 1 to 2.6 x 10(4) viable Candida albicans. Elimination of the microorganisms from the kidneys, liver, and spleen were evaluated by enumeration of colony ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1991.225
更新日期：1991-09-01 00:00:00
abstract：:Absorption of amino acids by the necturus proximal tubules was measured under free-flow conditions The coexistence of proximal tubular amino acid influx was determined by infusing saline into tubular lumens by the stopped-flow microperfusion technique. Under free-flow conditions, fractional absorption of individual am...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1978.67
更新日期：1978-06-01 00:00:00
abstract：:Augmentation of the CD36 pathway, which involves the uptake of several endogenous ligands including free fatty acids and oxidized low-density lipoprotein, contributes to the damage of proximal tubules and podocytes, whereas ablation of CD36 attenuates renal injury. Souza et al. demonstrate that 5A peptide can inhibit ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2016.01.009
更新日期：2016-04-01 00:00:00
abstract：:Studies were performed in anesthetized dogs (N = 7) to determine the effects of synthetic atrial natriuretic peptide (ANP) on sodium excretion in the presence and absence of control of glomerular filtration rate produced by suprarenal aortic clamping. Intrarenal infusion of synthetic atrial natriuretic peptide (0.3 mi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1986.144
更新日期：1986-07-01 00:00:00
abstract：BACKGROUND:Current renal substitution therapy for acute or chronic renal failure with hemodialysis or hemofiltration is life sustaining, but continues to have unacceptably high morbidity and mortality rates. This therapy is not complete renal replacement therapy because it does not provide active transport nor metaboli...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00486.x
更新日期：1999-06-01 00:00:00
abstract：:In summary, the three major segments of the collecting duct subserve three different functions in the urinary concentrating mechanism. The main function of the cortical collecting tubule is to raise the fractional solute contribution and absolute concentration of urea in fluid that it delivers to the outer medullary c...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.1987.41
更新日期：1987-02-01 00:00:00
abstract：:The binding of albumin to the glomerular capillary wall was studied using albumin-gold in perfused kidneys, the interaction of [3H]albumin with isolated glomeruli at 37 degrees C and 4 degrees C and the interaction at [3H]albumin with purified basement membrane. The albumin-gold was found to bind predominantly to the ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.149
更新日期：1995-04-01 00:00:00
abstract：:Although the goal glomerular filtration rate (GFR) for chronic dialysis initiation is currently above 5 ml/min per 1.73 m(2), there is no convincing evidence that patients will benefit from this approach. With close follow-up of advanced chronic kidney disease patients, aiming to start dialysis at an estimated GFR (eG...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2014.164
更新日期：2014-10-01 00:00:00
abstract：:Podocytes are highly differentiated cells that play an important role in maintaining glomerular filtration barrier integrity; a function regulated by small GTPase proteins of the Rho family. To investigate the role of Rho A in podocyte biology, we created transgenic mice expressing doxycycline-inducible constitutively...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.472
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND:The hypothesis that angiotensin-converting enzyme (ACE) inhibitors interfere with the treatment of anemia with recombinant human erythropoietin (rHuEPO) remains controversial. To test this hypothesis, a retrospective analysis was conducted in a large group of hemodialysis patients with renal anemia. METHODS...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00028.x
更新日期：2001-11-01 00:00:00
abstract：:Hypoxia in the embryo is a frequent cause of intra-uterine growth retardation, low birth weight, and multiple organ defects. In the kidney, this can lead to low nephron endowment, predisposing to chronic kidney disease and arterial hypertension. A key component in cellular adaptation to hypoxia is the hypoxia-inducibl...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2017.06.015
更新日期：2017-12-01 00:00:00
abstract：:Sudden death is one of the more frequent causes of death for hemodialysis patients, but the underlying mechanisms, contribution of arrhythmia, and associations with serum chemistries or the dialysis procedure are incompletely understood. To study this, implantable loop recorders were utilized for continuous cardiac rh...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究
doi：10.1016/j.kint.2017.11.019
更新日期：2018-04-01 00:00:00
abstract：:Electron microprobe analysis on freeze-dried cryosections was used to determine the effect of the loop diuretics torasemide and furosemide on intracellular electrolyte concentrations in individual cells of the outer and inner stripe of the outer medulla and on cell rubidium uptake, the latter a measure of basolateral ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.359
更新日期：1992-10-01 00:00:00
abstract：:Problems with vascular access are an important cause of morbidity and mortality in hemodialysis patients. We established a rodent model of arteriovenous fistula by anastomosing the end of a lateral vein to the side of the ventral artery of the rat tail. All operations were technically successful and in all animals the...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.207
更新日期：2008-08-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) are key mediators of adverse peritoneal membrane remodeling in peritoneal dialysis eventually leading to ultrafiltration failure. Both are pleiotropic growth factors with cell type-dependent regulation of expression and biological eff...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2013.217
更新日期：2013-12-01 00:00:00
abstract：:Mutations in the complement regulatory proteins are associated with several different diseases. Although these mutations cause dysregulated alternative pathway activation throughout the body, the kidneys are the most common site of injury. The susceptibility of the kidney to alternative pathway-mediated injury may be ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.02.036
更新日期：2016-07-01 00:00:00
abstract：:Identifying subjects with progressive chronic kidney disease will be important both in clinical practice and in conducting clinical trials. Pruijm et al. (in this issue) demonstrate for the first time that cortical oxygenation as evaluated by blood oxygenation level-dependent magnetic resonance imaging can predict fut...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2017.11.029
更新日期：2018-04-01 00:00:00
abstract：:Patients receiving cadaveric kidney transplants often experience delayed graft function. As iodinated contrast media injection (ICMI), necessary for cerebral angiography, which is often used to diagnose brain death, can be nephrotoxic, we compared renal function recovery (RFR) and 1-year and long-term graft survival a...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5001727
更新日期：2006-09-01 00:00:00
abstract：:Organomercurial diuretics stimulate renin secretion although the underlying cellular mechanisms remain undefined. Since organomercurials are also known to react with sulfhydryl groups, the present studies determined the effects of sulfhydryl reagents on renin secretion. The effects of the non-diuretic mercurial agent,...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.57
更新日期：1989-03-01 00:00:00
abstract：:Adenosine is known to decrease renal blood flow and glomerular filtration rate. We have tested the hypothesis that adenosine exerts contractile effects on mesangial cells. Furthermore, we have studied, using selective agonists and antagonists for adenosine, which kind of adenosine receptor, A1 or A2, is mainly implica...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.126
更新日期：1989-06-01 00:00:00
abstract：:Nitric oxide is generated from L-arginine by nitric oxide synthase (NOS), an enzyme that exists in several isoforms. Some studies found that a polymorphism (G894T) in the endothelial NOS gene was associated with decreased nitric oxide bioactivity and vascular complications. However, it is not known whether the enzyme ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.574
更新日期：2009-02-01 00:00:00
abstract：:Gene expression differs substantially among individual cells of the same type. We speculate that genes that are expressed in all but a portion of cells of a given cell type would be likely essential and required for either the cell survival (housekeeping) or for the cell type's unique structure and function, enabling ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2017.04.022
更新日期：2017-11-01 00:00:00
abstract：:Antibody responses to antigens are impaired in humans and animals with chronic renal failure (CRF), and parathyroid hormone (PTH) inhibits Staphylococcus aureus Cowan I (SAC) or pokeweed mitogen (PWM)-induced antibody production by B cells from normal subjects. Since CRF is associated with secondary hyperparathyroidis...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1991.286
更新日期：1991-11-01 00:00:00
abstract：:Low nephron number has been related to low birth weight and hypertension. In the southeastern United States, the estimated prevalence of chronic kidney disease due to hypertension is five times greater for African Americans than white subjects. This study investigates the relationships between total glomerular number ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000041
更新日期：2006-02-01 00:00:00
abstract：BACKGROUND:Tumor necrosis factor (TNF) receptor family members, such as Fas and TNF receptor 1 (TNFR1), are thought to induce apoptosis in a variety of cells and organs. Although a number of potential scenarios have been postulated for the involvement of these receptors in the pathogenesis of acute renal failure (ARF),...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00709.x
更新日期：2003-01-01 00:00:00
