Use of monoclonal antibodies (UCHL1, Ki-B3) against T and B cell antigens in routine paraffin-embedded skin biopsy specimens.

Abstract:

:Diagnosis and classification of cutaneous lymphoid infiltrates are often hampered by lack of frozen tissue to allow detailed immunophenotyping. Therefore antibodies that recognize antigens resistant to routine tissue processing would be of value. In this study we present a detailed analysis of two such antibodies against CD45R antigens, UCHL1 and Ki-B3, that detect predominantly T or B cells, respectively. Tumor cells in 125 cases of various nonlymphatic skin tumors did not react with either UCHL1 or Ki-B3. Examination of 85 biopsy specimens from 13 different inflammatory skin diseases demonstrates that most reactive T cells in the skin carry the UCHL1 antigen, as controlled by simultaneous immunophenotyping in frozen tissue. With few exceptions, UCHL1 also detected the tumor cells in 28 cases of cutaneous malignant T cell lymphomas. In contrast, Ki-B3 failed to detect tumor cells in seven of eight cases of malignant B cell lymphomas of the skin.

journal_name

J Am Acad Dermatol

authors

Sterry W,Hauschild A

doi

10.1016/s0190-9622(89)70155-9

subject

Has Abstract

pub_date

1989-07-01 00:00:00

pages

98-107

issue

1

eissn

0190-9622

issn

1097-6787

pii

S0190-9622(89)70155-9

journal_volume

21

pub_type

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