Abstract:
:The current study was performed to evaluate the mechanisms and therapeutic effects of overexpressing neuroglobin (Ngb) on spinal cord injury (SCI). Adeno-associated virus (AAV) was injected in the T12 section 7 days before SCI. Animals were randomly divided into four groups: a sham group, a vehicle group, an AAV-EGFP group and an AAV-Ngb group. Recovery of hind limb locomotor function was determined during the 3-week post operation period by the Basso, Beattie and Bresnahan locomotor rating scale. At 24 h after SCI and at the end of the study, the segments of spinal cord, centered with the lesion site were harvested for histopathological analysis. Immunofluorescence was performed using antibodies to recognize neuN in the lesion sections. At 24 h after SCI, the spinal cord tissue samples were removed to analyze tissue concentrations of superoxide dismutase (SOD) and malondialdehyde (MDA). Apoptotic cells were assessed using a terminal deoxynucleotidyl transferase, dUTP nick end labeling (TUNEL) kit. The expression of bcl-2, bax, cytochrome c, and cleaved caspase-3, were determined by Western blot assay and immunostaining analysis. The results showed that animals overexpressing Ngb had significantly greater recovery of locomotor function, less neuronal loss and fewer apoptotic cells. In addition, overexpressing Ngb significantly increased bcl-2 expression and SOD level, decreased bax expression, attenuated the release of cytochrome c from mitochondria to the cytosol fraction, and reduced the activity of caspase-3 and MDA level after SCI. These findings suggest, that overexpressing Ngb can significantly improve the recovery of locomotor function. This neuroprotective effect may be associated with the inhibition of neural apoptosis via the mitochondrial pathway.
journal_name
Brain Resjournal_title
Brain researchauthors
Lan WB,Lin JH,Chen XW,Wu CY,Zhong GX,Zhang LQ,Lin WP,Liu WN,Li X,Lin JLdoi
10.1016/j.brainres.2014.03.020subject
Has Abstractpub_date
2014-05-08 00:00:00pages
100-8eissn
0006-8993issn
1872-6240pii
S0006-8993(14)00368-0journal_volume
1562pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::A review of the available literature reveals that proliferation of ependyma occurs during embryological and early postnatal periods of development. Turnover, however, declines significantly during postnatal life and only low levels of residual activity persist into adulthood under normal conditions. In some regions of...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/0165-0173(85)90016-5
更新日期:1985-04-01 00:00:00
abstract::The study investigated the effect of aminoguanidine (AG) on inducible nitric oxide synthase (iNOS), aquaporin-4 (AQP4), malondialdehyde (MDA) and glutathione (GSH) levels in surgical brain injury (SBI) in rats. AG (75, 150 and 300 mg/kg, i.p.) was administered immediately following surgical resection. Using an SBI mod...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.05.041
更新日期:2009-07-28 00:00:00
abstract::The ventral tegmental area (VTA) is a brain region centrally involved in the development and expression of a variety of behaviors associated with drug use. Orexin/hypocretin (ox/hcrt) and corticotropin releasing factor (CRF) are both peptides released into the VTA, and an increasing number of studies show that both ox...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2009.10.068
更新日期:2010-02-16 00:00:00
abstract::Aquaporins (AQPs) are membrane proteins that facilitate water transport across biological membranes and are essential for the proper function of neural tissue. Although AQPs have been extensively studied in mammalian retina, their presence in lower vertebrate retina is less frequently characterized. AQP4 expressed in ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.11.014
更新日期:2012-01-13 00:00:00
abstract::Glycine is thought to be a major inhibitory neurotransmitter in the mammalian CNS. Two types of physiologically identified interneurons, Renshaw cells and Ia inhibitory interneurons, were intracellularly stained with horseradish peroxidase, and their axon terminals were studied at the electron microscopic level. Post-...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90590-r
更新日期:1991-04-26 00:00:00
abstract::The present study examined the effects of stereotaxic delivery of corticosterone to the amygdala on anxiety-like behavior and corticotropin-releasing factor (CRF) mRNA level in the central nucleus of the amygdala (CeA). Micropellets (30 microg) of crystalline corticosterone or cholesterol (control) were implanted bila...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02019-9
更新日期:2000-04-10 00:00:00
abstract::The purpose of this study was to examine mechanisms involved in the coupling of neuronal activity to cerebral blood flow (CBF). CBF was measured in rat cerebellum using laser-Doppler flowmetry during stimulus-evoked neuronal activity and related to the distribution of the extracellular field potential. Local electrica...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01354-7
更新日期:1996-02-26 00:00:00
abstract::The suspected functional role of dendritic spines as loci of neuronal plasticity (possibly memory and learning) is greatly enriched when active membrane properties are assumed at the spine head. Computations with reasonable electrical and structural parameter values (corresponding to an optimal range for spine stem re...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90333-6
更新日期:1985-01-28 00:00:00
abstract::The ability of diphenylhydantoin (DPH) to protect against hypoxia-induced neuronal damage was examined using electrophysiological recordings of extracellular evoked potentials from CA1 pyramidal neurons of rat hippocampal slices in vitro. In normal medium, a 15-min hypoxic insult (95% N2/5% CO2) produced rapid and com...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91501-q
更新日期:1991-04-19 00:00:00
abstract::Prolonged seizures have long been known to be associated with cell injury and cell death in brain. Such seizure-related neuronal injury has been assumed to be mediated by glutamate, the same excitatory amino acid in the central nervous system which propagates the seizure itself. Elevated extracellular concentrations o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00658-0
更新日期:1996-10-21 00:00:00
abstract::In the present study fast axonal transport was examined in streptozotocin rats with 4 weeks duration in diabetes. Tritiated leucine and 14C-labelled glucosamine were injected into the fifth lumbar ganglion and TCA-soluble as well as insoluble activity were measured in segments of the sciatic nerve at various time inte...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90631-0
更新日期:1979-09-14 00:00:00
abstract::We have developed and tested the biological activity and specificity of a novel fluorescent dextran-Texas Red-nerve growth factor (DTR-NGF) conjugate. DTR-NGF was found to promote survival and neurite outgrowth in cultured dissociated sympathetic neurons similarly to native NGF. The conjugate was taken up and transpor...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)03099-2
更新日期:2001-11-30 00:00:00
abstract::The neural manifestations of food avoidance learning in the mollusc, Pleurobranchaea, survive the surgical reduction of the preparation to the nearly isolated brain. These manifestations include increased synaptic inhibition and reduced synaptic excitation of the phasic paracerebral feeding command interneurons (PCps)...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91553-7
更新日期:1985-04-08 00:00:00
abstract::Tetanic stimulation of fibers in the hippocampal slice preparation produces long-term potentiation (LTP) and also decreases the in vitro incorporation of phosphate into the alpha subunit of pyruvate dehydrogenase (alpha PDH). This paper describes 6 experiments that were undertaken to replicate this observation. Hippoc...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91292-7
更新日期:1984-06-04 00:00:00
abstract::The blockade of the lateral parabrachial nucleus (LPBN) with GABA(A) receptor agonist muscimol induces robust hypertonic NaCl and water intake by rats. In the present study we investigated the effects of previous injections of losartan (AT(1) angiotensin receptor antagonist) into the LPBN on 0.3M NaCl and water intake...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.06.004
更新日期:2011-07-27 00:00:00
abstract::Current and voltage clamp recordings were made with a patch-clamp technique from large, light, dorsal root ganglia (DRG) neurons in tissue culture, derived from trisomy 16 and normal fetal mice. In a Na gradient of [52 mM]o/[28 mM]i, the action potential was accelerated, depolarization and repolarization were faster a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(88)90052-1
更新日期:1988-02-01 00:00:00
abstract::The omega-3 fatty acids are essential dietary nutrients and one of their important roles is providing the fatty acid with 22 carbons and 6 double bonds known as docosahexaenoic acid (DHA) for nervous tissue growth and function. Inadequate intakes of omega-3 fatty acids decrease DHA and increase omega-6 fatty acids in ...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2008.08.078
更新日期:2008-10-27 00:00:00
abstract::Immunohistochemistry for three calcium-binding proteins calbindin D-28k, calretinin, and parvalbumin revealed neuronal heterogeneity within the GP. Each neurone appeared to express either a single type of calcium binding protein or none at all. The co-localisation of calcium binding proteins was not observed. Combined...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)03263-2
更新日期:2002-03-08 00:00:00
abstract::A Rous Sarcoma virus transformed cerebellar cell line, BC6, secretes a factor which causes clonal glial cell lines to rapidly (1 h) extend processes. The factor shows a degree of specificity since only 3 out of 10 lines which exhibit either glial or combined glial and neuronal properties respond. The active factor app...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(83)90214-6
更新日期:1983-12-01 00:00:00
abstract::The effects of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS), a synthetic precursor of norepinephrine (NE), on the long-term potentiation (LTP) in the hippocampal mossy fiber-CA3 system was examined in urethane-anesthetized rats, the objective being to determine whether or not this drug acts as NE on the LTP. L-thr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90805-6
更新日期:1991-12-20 00:00:00
abstract::Hippocampal long-term potentiation (LTP) is considered as a cellular model of memory formation. Specific, electrical weak tetanization of distinct afferents such as the medial perforant path results in a short-lasting, protein synthesis-independent early-LTP (up to 4 h) within the dentate gyrus. A stronger tetanizatio...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.03.055
更新日期:2009-06-01 00:00:00
abstract::This study shows that permanent cell lines can be established from rat and mouse brain by direct tissue culture methodology without the aid of exogenous chemical or viral transforming agents. These cells were derived from specific areas of the brain, such as the cerebellum and hippocampus, at chosen times during fetal...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(77)91049-6
更新日期:1977-10-21 00:00:00
abstract::We tested the hypothesis that a single systemic injection of 380 mg/kg of the muscarinic agonist pilocarpine would produce more diffuse and severe seizure-induced brain damage than a single injection of lithium (3 mEq/kg) followed 4 h later by < 1/10 the dosage of pilocarpine. The hypothesis was not supported; the pat...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91126-6
更新日期:1994-06-20 00:00:00
abstract::Frequency-current curves for CA1 hippocampal pyramidal neurons are shown to have basically the same shape as those for spinal motoneurones, with a region of shallow slope at low frequencies, preceding a sleep linear or upward convex region at higher frequencies. The frequency range is, however, displaced towards lower...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91158-6
更新日期:1981-11-02 00:00:00
abstract::Seizures kindled with amygdaloid carbachol injections are transynaptic, dependent on activation of a specific population of muscarinic receptors, and some components of their expression could be mediated by intracellular second messengers. We measured cyclic GMP and cyclic AMP concentrations in micropunch biopsies of ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90637-2
更新日期:1986-11-12 00:00:00
abstract::We measured the release of neuropeptide Y (NPY) from hippocampal slices of rats at various times after limbic seizures induced by a subcutaneous injection of 12 mg/kg kainic acid (KA). Two days after KA, 100 mM KCl induced a 1.6 +/- 0.2-fold increase in NPY release compared to saline-injected rats (P < 0.05), while sp...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90847-8
更新日期:1994-10-10 00:00:00
abstract::Immunocytochemical and morphometric analyses were combined to demonstrate the presence of neuron subpopulations in the rat vestibular ganglion. Monoclonal antibodies reacting with neurofilament proteins (NF), calbindin-D 28K (CaBP) and calretinin (CaR) were used. Three subpopulations were identified: (1) CaBP- and CaR...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90334-6
更新日期:1992-06-05 00:00:00
abstract::Male rats were treated with barbital in their drinking fluid for about 40 weeks (daily dose 200 mg/kg). The treatment was stopped on day 0 and the number of muscarinic binding sites was measured in 3 brain parts (striatum; cortex and hippocampus; medulla oblongata and midbrain) during the abstinence with labeled quinu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90146-9
更新日期:1982-08-19 00:00:00
abstract::In an attempt to determine the basis for apparently conflicting reports of the effects of noradrenaline (NA) on the neurohypophyseal system and its effects on the parvocellular periventricular region of the paraventricular nucleus (PVN), recordings were made from the neurons in the supraoptic nucleus (SON) and the per...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90511-1
更新日期:1986-03-26 00:00:00
abstract::The properties of the beta amino acid transport system were examined in cultivated rat brain astrocytes, using the specific probe, beta-alanine. The uptake of beta-alanine is thought to be glial specific. Beta-alanine was not actively transported and the intracellular accumulation was not altered by coincubation with ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90856-3
更新日期:1982-05-06 00:00:00