Abstract：
BACKGROUND:Wilms' tumor 1 (WT1) gene has different functional properties depending on the isoform type. This gene correlates with cell proliferation in various types of cancer. Here, we investigated the expression of WT1 isoforms in breast cancer tissues, and focused on the oncogenic role through estrogen receptor-alpha (ER-α) and human epidermal growth factor receptor 2 (HER2). MATERIALS AND METHODS:Expression of WT1(17AA+) and (17AA-) was investigated in adjacent normal breast and breast cancer using Reverse transcription-polymerase chain reaction and western blotting. The correlation of WT1 isoforms with HER2 and ER-α was examined using MCF-7 cells stably-overexpressing WT1s and siRNA against WT1 gene. RESULTS:The expression of WT(17AA-) was significantly found in adjacent normal breast tissues. A mixture of WT1(17AA+) and WT1(17AA-) were highly expressed in breast carcinoma tissues. MCF-7 cells overexpressing WT1+/+ and WT1+/- represented strong expression of ER-α and HER2. Moreover, the silencing of WT1+/+ and WT1+/- resulted in a decrease of both ER-α and HER2 and led to a decrease of cell numbers. CONCLUSION:Our results suggest that WT1(17AA+) was exhibited dominantly in breast carcinoma tissues. WT1+/+ and WT1+/- correlated with the high expression of ER-α and HER2, leading to cell proliferation and might be involved in cancer development and progression.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Nasomyon T,Samphao S,Sangkhathat S,Mahattanobon S,Graidist Psubject
Has Abstractpub_date
2014-03-01 00:00:00pages
1333-42issue
3eissn
0250-7005issn
1791-7530pii
34/3/1333journal_volume
34pub_type
杂志文章abstract：BACKGROUND:The objective of the present study was to investigate how oxidative status influences the effectiveness of cytotoxicity of artemisinin towards cancer cells. It is hypothesized that antioxidants would reduce, whereas pro-oxidants would enhance, cytotoxicity. MATERIALS AND METHODS:Molt-4 human leukemia cells ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-04-01 00:00:00
abstract：BACKGROUND/AIM:The role of cathepsin-D is well established in breast cancer progression, being correlated with worse clinical outcomes. However, to our knowledge, no study has been performed investigating its expression in primary breast cancer tumors and their corresponding recurrences or metastasis. MATERIALS AND ME...
journal_title：Anticancer research
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更新日期：2012-03-01 00:00:00
abstract：BACKGROUND/AIM:The present study aimed to identify hypoxia-regulated microRNAs (HRMs) in vitro and investigate the clinical role of candidate HRMs in patients with gastroesophageal cancer (GEC). MATERIALS AND METHODS:microRNA expression changes induced by hypoxia in human GEC cell lines were measured with microarrays ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-02-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:For the selection of patients responding well to lentinan, we performed two immunological analysis of patients. Flow cytometric analysis was obtained from peripheral blood lymphocytes; the patients who had greater than 2.5 ratio of [CD11(-) CD8(+)/CD11(+) CD8(+) after treatment]/[CD11(-) CD8(+)/CD11(+) CD8(+) before t...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:E-selectin which is expressed on endothelial cells plays an important role in the adhesion of cancer cells to the vascular endothelium, being the ligand for a carbohydrate antigen expressed on cancer cells. In this study, the clinical usefulness of this protein was examined. E-selectin was expressed on the endothelial...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-08-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2001-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：10.21873/anticanres.11080
更新日期：2016-10-01 00:00:00
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journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1995-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13800
更新日期：2019-11-01 00:00:00
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pub_type： 杂志文章
doi：
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
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doi：
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doi：
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doi：
更新日期：2011-05-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2015-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2016-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
