当前位置： SCI文献检索 > ANTICANCER RESEARCH期刊下所有文献 > Morphological and phenotypic characterization of a new established ovarian carcinoma cell line (OvBH-1).

Morphological and phenotypic characterization of a new established ovarian carcinoma cell line (OvBH-1).

Abstract：

BACKGROUND:A limited number of permanent ovarian carcinoma cell lines have been described so far and the majority of them have been derived from ovarian ascitic fluid cells taken from patients with serous ovarian carcinoma usually after chemotherapy treatment. MATERIALS AND METHODS:The cells for culture were obtained from ascitic fluid cells of a patient with ovarian clear cell carcinoma. Cytomorphological analysis of cultured cells at early and late passages was performed by hematoxylin-eosin staining. Immunophenotypic characterization of cells was performed using the following monoclonal antibodies against: intermediated cellular filaments (CK 6/18, CK 7, CK 1,5,6,8,10,14,18, V9) ovarian carcinoma-associated antigens (OC125, OV-TL3), carcinoembryonic antigen, p53 and c-erbB-2 oncoproteins. RESULTS:In the established ovarian carcinoma cell line (OvBH-1) two morphologically distinct cell types were recognized. Cytomorphologically the dominating type appears to frankly malignant features. The second cell subtype showed a lower degree of malignant features. The epithelial origin of both cell types was confirmed by immunohistochemical staining using antibodies against different cytokeratin epitopes. The expression of tumor-associated antigens (CA125, OV-TL3) was found in both cell subtypes reflecting their origin from ovarian carcinoma. The cell line was negative for CEA staining. The genetic defects of cultured cells were revealed by detection of p53 and c-erbB-2 overexpression. The level of both oncoproteins and especially c-erbB-2 was higher in the cell subtype with frankly malignant morphological features. CONCLUSIONS:A new established, well characterized ovarian clear cell carcinoma line OvBH-1 provides an experimental model for further investigation of the biological alterations responsible for carcinogenesis and chemoresistance of this uncommon subtype of epithelial ovarian carcinomas.

journal_name

Anticancer Res

journal_title

Anticancer research

authors

Bar JK,Harlozińska A

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

2975-80

issue

5A

eissn

0250-7005

issn

1791-7530

journal_volume

20

pub_type

杂志文章

相关文献

ANTICANCER RESEARCH文献大全
  • Angiogenesis: prognostic significance in laryngeal cancer.

    abstract：:The aim of this study was to evaluate the role of angiogenesis in the progression of laryngeal squamous cell carcinoma (LSCC). We correlated disease-free survival with microvessel count (MC) in the hot spot areas of 97 randomly selected caucasian males with LSCC followed for 60 to 90 months after surgery with or witho...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Beatrice F,Cammarota R,Giordano C,Corrado S,Ragona R,Sartoris A,Bussolino F,Valente G

    更新日期：1998-11-01 00:00:00

  • Preclinical Evaluation of Combined Topoisomerase and Proteasome Inhibition Against Pediatric Malignancies.

    abstract：BACKGROUND/AIM:The toxicity of the proteasome inhibitor MG132 was tested alone and combined either with the topoisomerase I inhibitor topotecan or the topoisomerase II inhibitor etoposide against a panel of 18 cell lines representing six pediatric tumor types. MATERIALS AND METHODS:MG132, topotecan, etoposide and thei...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：10.21873/anticanres.12684

    authors： Destanovic E,Boos J,Lanvers-Kaminsky C

    更新日期：2018-07-01 00:00:00

  • The possible role of stromal influences in tumor cell heterogeneity. Histology and fibronectin pattern of the Yoshida sarcoma of the rat.

    abstract：:Yoshida tumors implanted subcutaneously in the rat are conspicuously heterogeneous. A definite zoning can be discerned. Cell nuclei range from uniform epitheloid (type I) via pleomorphic (type II) to compact (type III). Type III is seen to detach at the rim of the tumor and to infiltrate the dermis. Type I is characte...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Jiwa M,Van den Hooff A

    更新日期：1985-05-01 00:00:00

  • A 12-year Recurrence-free Survival After Multidisciplinary Treatment for a Patient With Combined Hepatocellular-Cholangiocarcinoma.

    abstract：:We report a 64-year-old woman with a 9-cm liver tumor in the left lateral section. The patient had neither hepatitis B or C virus infection, nor cirrhosis. Carbohydrate antigen 19-9 (CA 19-9) level was 1,889 U/ml. We also suspected bulky hilar lymph node metastasis, and a left lateral sectionectomy without lymph node ...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：10.21873/anticanres.13327

    authors： Miyata T,Beppu T,Imamura YU,Hayashi H,Imai K,Chikamoto A,Yamashita YI,Fukubayashi K,Ishiko T,Baba H

    更新日期：2019-04-01 00:00:00

  • Selective cytotoxicity of L-glutamic acid gamma-monohydroxamate (GAH) for melanoma tumor cells.

    abstract：:We have previously shown that L-glutamic acid gamma-monohydroxamate (GAH) exhibits an antitumor activity, both in vitro and in vivo. In this report we explore the selective cytotoxicity of GAH in vitro by comparing the survival of tumor and normal cells. GAH exerts an irreversible delayed effect with tumoral cells and...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Thomasset N,Chabanas A,Tournaire R,Malley S,Navarro C,Lawrence JJ,Doré JF,Vila J

    更新日期：1993-09-01 00:00:00

  • Early-stage central lung cancer and volumetric modulated arc therapy: a dosimetric case study with literature review.

    abstract：BACKGROUND/AIM:In the present article we review on the use of Volumetric Modulated Arc Therapy (VMAT) for a small lung nodule that was centrally located in close proximity to the mediastinal structures. CASE REPORT:An inoperable patient with central, clinical stage IA adenocarcinoma of the right lung was treated with ...

    journal_title：Anticancer research

    pub_type： 杂志文章,评审

    doi：

    authors： Valakh V,Chan P,D'Adamo K,Micaily B

    更新日期：2013-10-01 00:00:00

  • Peritoneal Metastases in a Patient-derived Orthotopic Xenograft (PDOX) Model of Colon Cancer Imaged Non-invasively via Red Fluorescent Protein Labeled Stromal Cells.

    abstract：BACKGROUND/AIM:Patient-derived orthotopic xenograft (PDOX) models have patient-like clinical features and may be imaged, in case of some cancers, by passaging of the tumors through transgenic nude mice expressing red-fluorescent protein (RFP) where they stably acquire RFP expressing stroma. The aim of the present study...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：10.21873/anticanres.13492

    authors： Park JH,Zhao M,Oshiro H,Miyake K,Higuchi T,Reynoso J,Razmjooei S,Bouvet M,Clary B,Zhang Z,Sugisawa N,Yamamoto J,Singh SR,Hoffman RM

    更新日期：2019-07-01 00:00:00

  • R-verapamil decreases anti-estrogen resistance in a breast cancer model.

    abstract：:Drug resistance eventually limits the effectiveness of antiestrogens in breast cancer treatment. Pharmacological reversal of this refractoriness has been attempted with R-Verapamil, a well tolerated calcium channel blocker. This drug significantly decreased the incidence of lung foci after intravenous seeding of the R...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Kellen JA,Wong A,Georges E,Ling V

    更新日期：1991-03-01 00:00:00

  • Development of a Questionnaire for Monitoring Risk Factors for Chemotherapy-induced Nausea and Vomiting - A NOGGO Pilot Study.

    abstract：BACKGROUND:Despite many years of clinical research and development, nausea and vomiting remain challenging toxicities related to chemotherapy. The aim of our study was to clarify the significance of non-pharmacological, patient-related risk factors for chemotherapy-induced nausea and vomiting. Furthermore, we aimed to ...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：10.21873/anticanres.12798

    authors： Rittmeister H,Oskay-Özcelik G,Richter R,Sehouli J,Grabowski JP

    更新日期：2018-08-01 00:00:00

  • Correlation of Pathological Complete Response With Tumor Volume Reduction During Neoadjuvant Chemoradiotherapy in Lung Cancer.

    abstract：BACKGROUND/AIM:We retrospectively investigated the relationship between pathological complete response (pCR) and tumor volume (TV) reduction during neoadjuvant chemoradiation therapy (NACRT) in non-small cell lung cancer (NSCLC). PATIENTS AND METHODS:We evaluated patients who received NACRT (50 Gy/25 fractions with pl...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：10.21873/anticanres.14435

    authors： Takahashi S,Go T,Anada M,Kinoshita T,Nishide T,Yokomise H,Shibata T

    更新日期：2020-08-01 00:00:00

  • The bisphosphonate, zoledronic acid reduces experimental neuroblastoma growth by interfering with tumor angiogenesis.

    abstract：BACKGROUND:Zoledronic acid is a new member of the bisphosphonate (BP) class of compounds, a family of closely related synthetic molecules originally derived from the naturally occurring pyrophosphate. These compounds that are potent inhibitors of bone resorption, have been shown to reduce the growth of several cancer c...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Bäckman U,Svensson A,Christofferson RH,Azarbayjani F

    更新日期：2008-05-01 00:00:00

  • Glucose transporter GLUT1 in colorectal adenocarcinoma cell lines is inversely correlated with tumour cell proliferation.

    abstract：:The high glucose consumption of tumour cells has been well known since Warburg's investigations. Nevertheless, the reason for this phenomenon is still a matter of speculation. Glucose uptake of tumour cells is mainly mediated by GLUT1. In this study, the question of whether glucose uptake as well as expression of GLUT...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Hauptmann S,Grünewald V,Molls D,Schmitt WD,Köbel M,Kriese K,Schürmann A

    更新日期：2005-09-01 00:00:00

  • Age as a prognostic factor in breast cancer.

    abstract：:We retrospectively analysed data from 1751 patients with non-metastatic and non-inflammatory breast cancer treated in our institution between 1977 and 1989, in order to evaluate the link between age and prognosis in breast cancer. We chose three age groups (1): < or = 33 (n = 67), 34-40 ( n = 155), > 40 years (n = 152...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Vanlemmens L,Hebbar M,Peyrat JP,Bonneterre J

    更新日期：1998-05-01 00:00:00

  • Effects of 5-fluorouracil on various melanoma cell metabolisms. Its use for assessing the cold thymidine pool.

    abstract：:5-Fluorouracil (5FU) increases the rate of incorporation of tritiated thymidine (3HTdR) and iododeoxyuridine (125 IUdR) into the cell and its DNA. 5FU has differential effect on metabolism of DNA through its specific action on thymidylate synthetase. Effects of 5FU on the incorporation of other metabolic precursors we...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Vercammen-Grandjean AF,Arnould R,Libert A,Lejeune FJ

    更新日期：1982-05-01 00:00:00

  • MIB1 (Ki-67) expression in non-Hodgkin's lymphomas.

    abstract：:The MIB1 monoclonal antibody which is used as a marker of cell proliferation was studied by immunohistochemistry on formalin-fixed and paraffin embedded biopsy specimens of lymph nodes in 40 high- and 46 lowgrade cases of non-Hodgkin's lymphomas (NHL) classified according to the Kiel classification. All cases were fou...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Kalogeraki A,Tzardi M,Panagiotides I,Koutsoubi K,Bolioti S,Rontogianni D,Stefanaki K,Zois E,Karidi E,Darivianaki K,Delides G,Kanavaros P

    更新日期：1997-01-01 00:00:00

  • Prediction of Non-sentinel Lymph Node Metastases After Positive Sentinel Lymph Nodes Using Nomograms.

    abstract：BACKGROUND/AIM:Only 30-50% of patients with sentinel lymph node (SLN) metastases present with further axillary lymph node metastases. Therefore, up to 70% of patients with positive SLN are overtreated by axillary dissection (AD) and may suffer from complications such as sensory disturbances or lymphedema. According to ...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：10.21873/anticanres.12694

    authors： Gruber I,Henzel M,Schönfisch B,Stäbler A,Taran FA,Hahn M,Röhm C,Helms G,Oberlechner E,Wiesinger B,Nikolaou K,LA Fougère C,Wallwiener D,Hartkopf A,Krawczyk N,Fehm T,Brucker S

    更新日期：2018-07-01 00:00:00

  • A combination phase I study of weekly paclitaxel and doxifluridine in advanced gastric cancer patients.

    abstract：BACKGROUND:Preclinical studies have shown that paclitaxel and doxifluridine can act synergistically without overlapping toxicity for the treatment of advanced gastric cancer. The objectives of this study were to determine the maximum tolerated dose (MTD), the dose-limiting toxicity and the recommended Phase II dose for...

    journal_title：Anticancer research

    pub_type： 临床试验,杂志文章

    doi：

    authors： Takeyoshi I,Makita F,Tanahashi Y,Yokomori T,Iwazaki S,Kawashima Y,Iwanami K,Yamada T,Kawate S,Hamada K,Sunose Y,Yoshida M,Horiguchi J,Iesato H,Kobayashi M,Morishita Y

    更新日期：2005-03-01 00:00:00

  • Serially cultured keratinocytes from human scalp hair follicles: a tool for cytogenetic studies.

    abstract：:Keratinocytes originating from adult human hair follicles, the most convenient biopsy tissue, can be serially cultured using a combination of two techniques. Primary cultures are established using plucked scalp hair follicles and the bovine eye lens capsule as a growth substrate. Subsequently, cells from these culture...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Weterings PJ,Roelofs HM,Jansen BA,Vermorken AJ

    更新日期：1983-05-01 00:00:00

  • Detection and clinical relevance of tumor cells in blood and bone marrow of patients with colorectal cancer.

    abstract：:In colorectal cancer, the predictive value of the currently used staging method is limited. Therefore, many parameters have been studied to improve the prediction of final clinical outcome, including several aspects of the primary tumor that are associated with its aggressiveness and the capacity of the host response....

    journal_title：Anticancer research

    pub_type： 杂志文章,评审

    doi：

    authors： Vlems FA,Wobbes T,Punt CJ,Van Muijen GN

    更新日期：2003-01-01 00:00:00

  • Promoter Methylation Down-regulates B-cell Translocation Gene 1 Expression in Breast Carcinoma.

    abstract：BACKGROUND/AIM:The mechanism responsible for B-cell translocation gene 1 (BTG1) down-regulation in breast carcinoma remains unknown. We examined the BTG1 expression status in breast carcinoma cells and investigated the mechanism underlying the observed alterations. MATERIALS AND METHODS:Four breast carcinoma cell line...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：10.21873/anticanres.13729

    authors： Woo HY,DO SI,Kim SH,Song SY,Kim HS

    更新日期：2019-10-01 00:00:00

  • Activating Gs a mutation rarely occurs in musculoskeletal tumors other than fibrous dysplasia.

    abstract：BACKGROUND:Activating Gs a mutations have been identified in most instances of fibrous dysplasia (FD). This mutation leads to consistently elevated intracellular cyclic adenosine monophosphate (cAMP) levels, with various biological consequences. The development of secondary sarcoma in FD is a rare but well-established ...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Gu W,Ogose A,Matsuba A,Kawashima H,Hotta T,Kudo N,Hoshino M,Kondo N,Mera H,Endo N

    更新日期：2006-03-01 00:00:00

  • Influence of hypotonic stress on the biliary excretion of doxorubicin in Wistar and TR- rats.

    abstract：:In tumor cells, doxorubicin (DOX) is excreted by P-glycoprotein (P-gp) and the multidrug resistance-associated protein (mrp). Both transporters might also be involved in cellular regulatory volume decrease (RVD). To study the hepatobiliary excretion of DOX during RVD, isolated livers of Wistar and multidrug resistance...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Gaugg M,Haslmayer P,Jäger W,Thalhammer T

    更新日期：2001-07-01 00:00:00

  • Radiation therapy for oncological emergencies.

    abstract：AIMS AND BACKGROUND:Radiation therapy holds a fundamental role in oncological emergencies such as superior vena cava syndrome, spinal cord compression and endocranial hypertension. The purpose of our study was, by comparing schedules of treatment, to confirm the efficacy of hypofractionated radiation therapy. METHODS:...

    journal_title：Anticancer research

    pub_type： 临床试验,杂志文章

    doi：

    authors： Donato V,Bonfili P,Bulzonetti N,Santarelli M,Osti MF,Tombolini V,Banelli E,Enrici RM

    更新日期：2001-05-01 00:00:00

  • Keishi-ka-kei-to, a traditional Chinese herbal medicine, inhibits pulmonary metastasis of B16 melanoma.

    abstract：:Keishi-ka-kei-to, a traditional Chinese herbal medicine composed of a mixture of crude extracts from five medicinal plants (Cinnamomi cortex, Paeoniae radix, Zizyphi fructus, Zingiberis rhizoma and Glycyrrhizae radix), inhibited experimental pulmonary metastasis in mice implanted with B16F10 melanoma cells. When 136 t...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Suzuki F,Kobayashi M,Komatsu Y,Kato A,Pollard RB

    更新日期：1997-03-01 00:00:00

  • Clinical experience with bevacizumab in colorectal cancer.

    abstract：:Angiogenesis, the process of generating new capillary blood vessels, is a fundamental requirement for normal physiological processes including embryogenesis, reproductive function and wound healing. Angiogenesis is also implicated in various pathological conditions including age-related retinal macular degeneration, d...

    journal_title：Anticancer research

    pub_type： 杂志文章,评审

    doi：

    authors： Salesi N,Bossone G,Veltri E,Di Cocco B,Marolla P,Pacetti U,Larosa G,Muni R,Vecchione A

    更新日期：2005-09-01 00:00:00

  • Cryosurgery plus adjuvant systemic alpha2-interferon for HPV-associated lesions.

    abstract：OBJECTIVE:The authors report their experience in patients with adjuvant systemic 2-interferon with the aim of defining the effectiveness, side-effects, indications and limitations of this treatment. MATERIALS AND METHODS:From January 1989 to December 1996, 123 patients with genital, anorectal and perineal HPV lesions ...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Scala M,Bonelli G,Gipponi M,Margarino G,Muzza A

    更新日期：2002-03-01 00:00:00

  • The co-expression of c-myc and p53 increases and reaches a plateau early in oral oncogenesis.

    abstract：BACKGROUND:The balance between cell proliferation and apoptosis plays a significant role in cancer development. The expressions of the p53 and c-myc genes, both strongly related to cell proliferation and apoptosis, were studied in sequential histological grades of oral carcinogenesis in an animal model. MATERIALS AND ...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Papakosta V,Vairaktaris E,Vylliotis A,Derka S,Nkenke E,Vassiliou S,Lazaris A,Mourouzis C,Rallis G,Spyridonidou S,Anagnostopoulou S,Perrea D,Donta I,Yapijakis C,Patsouris E

    更新日期：2006-07-01 00:00:00

  • Epidermal growth factor receptor changes during breast cancer metastasis.

    abstract：BACKGROUND:Since the anti-HER2 monoclonal antibody trastuzumab made its triumphant advance into breast cancer therapy, new selective agents, including pan-HER inhibitors are entering clinical practice. MATERIALS AND METHODS:This study investigates the expression of the four HER-family members, HER1-4, in 48 primary br...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Fuchs IB,Siemer I,Bühler H,Schmider A,Henrich W,Lichtenegger W,Schaller G,Kuemmel S

    更新日期：2006-11-01 00:00:00

  • Prediction of response in cancer immunotherapy.

    abstract：:This review focuses on the relationship between pre-treatment immune parameter values and outcome of immunotherapy of cancer patients. The evidence presented in this review suggests that there is a relationship between pre-treatment immune parameter values and survival of cancer patients treated with immunotherapy. Tu...

    journal_title：Anticancer research

    pub_type： 杂志文章,评审

    doi：

    authors： Characiejus D,Jacobs JJ,Pašukonienė V,Kazlauskaitė N,Danilevičiūtė V,Mauricas M,Den Otter W

    更新日期：2011-02-01 00:00:00

  • Increased alternative lengthening of telomere phenotypes of telomerase-negative immortal cells upon trichostatin--a treatment.

    abstract：:Human immortal cells maintain their telomeres either by telomerase or by alternative lengthening of telomeres (ALT) that is based on homologous telomeric recombination. Previous studies showed that the ALT mechanism is activated in non-ALT cells when heterochromatic features are reduced. In this study, we examined the...

    journal_title：Anticancer research

    pub_type： 杂志文章

    doi：

    authors： Jung AR,Yoo JE,Shim YH,Choi YN,Jeung HC,Chung HC,Rha SY,Oh BK

    更新日期：2013-03-01 00:00:00