Abstract：
BACKGROUND:Oestrogen (E2) induces apoptosis in long-term E2-deprived MCF7 cells (MCF7:5C). Taxanes have been used extensively in the treatment of early and advanced breast cancer. We have interrogated the sequence of events that involve the apoptotic signalling pathway induced by E2 in comparison with paclitaxel. METHODS:DNA quantification and cell cycle analysis were used to assess proliferation of cancer cells. Apoptosis was evaluated using annexin V and DNA staining methods. Regulation of apoptotic genes was determined by performing PCR-based arrays and RT-PCR. RESULTS:E2-induced apoptosis is a delayed process, whereas paclitaxel immediately inhibits the growth and induces death of MCF7:5C cells. The cellular commitment for E2-triggered apoptosis occur after 24 h. Activation of the intrinsic pathway was observed by 36 h of E2 treatment with subsequent induction of the extrinsic apoptotic pathway by 48 h. Paclitaxel exclusively activated extramitochondrial apoptotic genes and caused rapid G2/M blockade by 12 h of treatment. By contrast, E2 causes an initial proliferation with elevated S phase of cell cycles followed by apoptosis of the MCF7:5C cells. Most importantly, we are the first to document that E2-induced apoptosis can be reversed after 24 h treatment. CONCLUSIONS:These data indicate that E2-induced apoptosis involves a novel, multidynamic process that is distinctly different from that of a classic cytotoxic chemotherapeutic drug used in breast cancer.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Obiorah I,Sengupta S,Fan P,Jordan VCdoi
10.1038/bjc.2014.50subject
Has Abstractpub_date
2014-03-18 00:00:00pages
1488-96issue
6eissn
0007-0920issn
1532-1827pii
bjc201450journal_volume
110pub_type
杂志文章abstract：:NIH3T3 cells transfected with c-H-ras and/or c-myc genes were examined for differences in drug sensitivity. The five transfectants used were N8, NIH3T3-nm-1, pT22-3-nm-2, pP1-4 and pT22-3. They were transfected with pKOneo alone, pKOneo and c-myc, pKOneo and c-myc plus activated c-H-ras, normal c-H-ras and activated c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1991.56
更新日期：1991-02-01 00:00:00
abstract：:The response of a transplantable rat fibrosarcoma, RIB(5), to a single injection of 100 mg/kg body weight of cyclophosphamide, 0·5, 1, 2 and 3 days after treatment, has been studied by means of a morphometric analysis of tumour sections and has been compared to the changes occurring in untreated tumours. The results s...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1973.9
更新日期：1973-01-01 00:00:00
abstract：:Four human melanoma xenograft lines (A-07, D-12, R-18, U-25) grown orthotopically in Balb/c nu/nu mice were characterized with respect to the fraction of radiobiologically hypoxic cells. The purpose of the study was to establish a firm radiobiological basis for future use of the lines in the development and evaluation...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.598
更新日期：1998-10-01 00:00:00
abstract：:We analysed two case-control studies of women from Italy and Switzerland, including 195 cases of oral and pharyngeal cancers and 1113 controls. The multivariate odds ratio was 4.6 for heavy smokers and 2.7 for high alcohol intake. Vegetables, fruit, beta-carotene and wholegrain foods were inversely, butter and retinol...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.1999.0900
更新日期：2000-01-01 00:00:00
abstract：:Histological sections of the primary tumour and of homolateral axillary lymph nodes from 500 women with operable invasive breast cancer have been examined. The tumours have been graded and the degree of round cell infiltration assessed. These features, together with clinical palpability and pathological involvement of...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1972.19
更新日期：1972-04-01 00:00:00
abstract：:The tumour suppressor gene CDKN2A, located on chromosome 9p21, encodes the cell cycle regulatory protein p16. Inactivation of the CDKN2A gene could lead to uncontrolled cell growth. In order to determine the role of CDKN2A in the development of sporadic ovarian cancer, loss of heterozygosity at 9p21-22, homozygous del...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690621
更新日期：1999-08-01 00:00:00
abstract：BACKGROUND:A Population-Based Cancer Registry (PBCR) was set up in Sikkim (a state in the North Eastern India) in 2003. We examined incidence rates by ethnic groups from 2003-2008. METHODS:Age-adjusted incidence rates (AARs) per 100,000 person-years were calculated by direct method using the world standard population,...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.598
更新日期：2012-02-28 00:00:00
abstract：:The limited prognosis of patients with castration-resistant prostate cancer (CRPC) on existing hormonal manipulation therapies calls out for the urgent need for new management strategies. The novel, orally available, small-molecule compound, abiraterone acetate, is undergoing evaluation in early clinical trials and em...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/sj.bjc.6604904
更新日期：2009-03-10 00:00:00
abstract：:A retrospective analysis of 321 gastric cancer patients was made to assess the prognostic value of TNM classification, tumour differentiation, Laurén classification, proliferative rate, inflammatory reaction and tumour invasion in vascular or neural structures of the gastric wall. The TNM classification showed the str...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.434
更新日期：1996-09-01 00:00:00
abstract：:Meningiomas are tumours derived from the arachnoid and pia mater. During embryogenesis, these membranes develop from the migrating craniofacial neural crest. We have previously demonstrated that meningiomas have characteristic features of embryonic meninges. Craniofacial neural crest derivatives are affected during no...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690705
更新日期：1999-10-01 00:00:00
abstract：:We investigated expression of a human p53-inducible gene, P2XM, a member of the P2X-receptor family of ATP-gated ion channels, in 56 human primary soft-tissue tumours including 47 sarcomas and nine benign tumors. Among the 47 sarcomas examined by reverse transcription polymerase chain reaction, 12 had lost expression ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690484
更新日期：1999-06-01 00:00:00
abstract：:Seventy three children with rhabdomyosarcoma were treated by members of the Children's Solid Tumour Group during the period, 1974-1981. The extent of disease at diagnosis was found to be the major influence affecting outcome. Children with tumours confined to the tissue of origin with no evidence of nodal or metastati...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1983.175
更新日期：1983-08-01 00:00:00
abstract：:Human papillomavirus (HPV) is thought to cause some vulval squamous cell carcinomas (VSCC) by degrading p53 product. Evidence on whether HPV-negative VSCC results from p53 mutation is conflicting. We performed immunohistochemistry for p53 product on 52 cases of lone vulval intraepithelial neoplasia (VIN), 21 cases of ...
journal_title：British journal of cancer
pub_type： 杂志文章,meta分析,多中心研究
doi：10.1038/sj.bjc.6600677
更新日期：2003-01-27 00:00:00
abstract：BACKGROUND:Heterogeneity and low incidence comprise the biggest challenge in sarcoma diagnosis and treatment. Chemotherapy, although efficient for some sarcoma subtypes, generally results in poor clinical responses and is mostly recommended for advanced disease. Specific genomic aberrations have been identified in some...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-018-0359-4
更新日期：2019-02-01 00:00:00
abstract：:The relationship between hormone replacement treatment (HRT) and breast cancer risk was analysed using data from a case-control study conducted between June 1991 and February 1994 in six Italian centres on 2569 patients aged below 75 with histologically confirmed breast cancer and 2588 controls admitted to hospital fo...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.310
更新日期：1995-07-01 00:00:00
abstract：BACKGROUND:The recently described navigator proteins have a multifaceted role in cytoskeletal dynamics. We report here on the relevance of one of them, navigator 3 (NAV3), in colorectal cancer (CRC). METHODS:We analysed changes in chromosome 12 and NAV3 copy number in CRC/adenoma samples of 59 patients and in 6 CRC ce...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.553
更新日期：2012-01-31 00:00:00
abstract：BACKGROUND:To date, no good marker for screening or disease monitoring of endometrial cancer (EC) is available. The aims of this study were to investigate HE4 gene, protein expression and serum HE4 (sHE4) levels in a panel of ECs and normal endometria (NEs) and to correlate sHE4 with patient clinicopathological charact...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.109
更新日期：2011-04-26 00:00:00
abstract：:The relationship between aspirin use and colorectal cancer risk was examined by a case-control study in Italy. Regular aspirin use was reported by only 47 (3.5%) cases and 77 (4.1%) control subjects, giving a multivariate odds ratio (OR) of 0.7 (95% CI 0.5-1.0) after allowance for education, physical exercise and sele...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.444
更新日期：1997-01-01 00:00:00
abstract：:Male and female Swiss strain mice were put on a 0.03% thioacetamide diet at the age of 2 months. Control mice were kept on a stock diet. Control and treated mice were killed at the ages of 6, 9, 13 and 17 months. Progressive morphological, histological and biochemical alterations in the liver and tumour tissue of the ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1970.60
更新日期：1970-09-01 00:00:00
abstract：:Ductal carcinoma in situ (DCIS) now represents 20-25% of all 'breast cancers' consequent upon detection by population-based breast cancer screening programmes. Currently, all DCIS lesions are treated, and treatment comprises either mastectomy or breast-conserving surgery supplemented with radiotherapy. However, most D...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/s41416-019-0478-6
更新日期：2019-08-01 00:00:00
abstract：BACKGROUND:Vascular endothelial growth factor (VEGF)-mediated angiogenesis mediates tumour growth and metastasis. Meningiomas are primarily benign, slow-growing, highly vascularised tumours. Aside from VEGF, there is little data on the function of major angiogenic proteins in meningiomas. METHODS:The VEGFA, platelet-d...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.459
更新日期：2012-11-06 00:00:00
abstract：:The oncogenicity of Duck hepatitis B virus (DHBV) is unclear since hepatocellular carcinomas (HCCs) have been reported only in domestic ducks in Qidong, an area of China where hepatitis B virus (HBV) and aflatoxin B1 (AFB1) are risk factors for liver cancer in man. In order to better define the association between DHB...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.16
更新日期：1994-01-01 00:00:00
abstract：:Vascular endothelial growth factor-C (VEGF-C) functions specifically to induce lymphangiogenesis. We examined the relationship between expression of VEGF-C and clinicopathological features in patients with colorectal cancer. The expression of VEGF-C in the 99 primary tumours and 18 metastatic lymph nodes from colorect...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2000.1396
更新日期：2000-10-01 00:00:00
abstract：:The lymphocyte marker pattern of non-Hodgkin's lymphoma cells was related to current concepts of lymphoma classification. In a series of 28 lymphomas lymphocyte markers indicated that 2 were of histiocytic origin, 2 were unclassifiable, none were derived from T cells and the remainder were B-cell neoplasms. The immuno...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.154
更新日期：1977-07-01 00:00:00
abstract：:In a blind study, 518 serum samples were assayed for serum levels of mammary serum antigen (MSA) by an enzyme immunoassay (EIA) using the 3E1.2 monoclonal antibody. Using 300 IU as the arbitrary cut off to distinguish normal from abnormal individuals, 75% of patients with primary Stage I carcinoma of the breast (n = 1...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1988.66
更新日期：1988-03-01 00:00:00
abstract：:We analysed 29 consecutive cases of primary gastric lymphoma (20 men and 9 women) treated in our unit between January 1977 and May 1983. Median age was 55 years. Abdominal pain and weight loss were the main presenting symptoms while there was no palpable disease in the majority of cases. Upper gastrointestinal radiolo...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1985.206
更新日期：1985-09-01 00:00:00
abstract：BACKGROUND:defective DNA repair has a causal role in hereditary colorectal cancer (CRC). Defects in the base excision repair gene MUTYH are responsible for MUTYH-associated polyposis and CRC predisposition as an autosomal recessive trait. Numerous reports have suggested MUTYH mono-allelic variants to be low penetrance ...
journal_title：British journal of cancer
pub_type： 杂志文章,meta分析
doi：10.1038/sj.bjc.6605966
更新日期：2010-12-07 00:00:00
abstract：BACKGROUND:Efficacy and safety are the two considerations when characterising the effects of a new therapy. We sought to apply an innovative method of assessing the benefit-risk balance using data from a completed randomised controlled trial that compared erlotinib vs placebo added to gemcitabine in patients with advan...
journal_title：British journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1038/bjc.2015.55
更新日期：2015-03-17 00:00:00
abstract：BACKGROUND:The importance of lymphocyte subtypes in determining outcome in primary operable ductal invasive breast cancer remains unclear. The aim of present study was to examine the relationship between tumour lymphocyte subsets infiltrate and standard clinico-pathological factors and survival in patients with primary...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.493
更新日期：2013-09-17 00:00:00
abstract：:Human dialysable Transfer Factor (TFd) extracted from lymphocytes of patients with transitional cell carcinoma of bladder (TCCB) was replicated in culture by lymphoblastoid cell lines. The effectiveness of two such TFdLs produced in vitro in transferring sensitivity to TCCB was assessed in the lymphocyte migration tes...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1976.98
更新日期：1976-06-01 00:00:00
