当前位置： SCI文献检索 > JOURNAL OF DERMATOLOGICAL SCIENCE期刊下所有文献 > Application of FTIR microspectroscopy for characterization of biomolecular changes in human melanoma cells treated by sesamol and kojic acid.

Application of FTIR microspectroscopy for characterization of biomolecular changes in human melanoma cells treated by sesamol and kojic acid.

Abstract：

BACKGROUND:Hyperpigmentation is aesthetic undesirable. Sesamol and the standard antimelanogenic agent (kojic acid) were shown to hinder melanogenesis by blocking tyrosinase and reducing melanin content. OBJECTIVE:The FTIR microspectroscopy was used in an attempt to find a novel method to define biological alternation in a melanogenesis inhibition of sesamol and kojic acid. METHODS:Tyrosinase inhibition and melanin content of sesamol and kojic acid were evaluated. The FTIR microspectroscopy was adopted to define the vibrational characteristic involved with the melanogenesis in the untreated SK-MEL2 cells vs. the sesamol- and kojic-treated SK-MEL2 cells. RESULTS:Sesamol and kojic acid inhibited mushroom tyrosinase at IC₅₀ of 0.33 μg/ml and 6.1±0.4 μg/ml, respectively. Moreover, 30 μg/ml sesamol inhibited 23.55±8.25% cellular tyrosinase activity in human SK-MEL2 cells, while 600 μg/ml kojic acid inhibited 33.9±1.4% cellular tyrosinase activity in the same cells. In the SK-MEL2-treated with two inhibitors, the FTIR spectra assigned to the lipid and nucleic acid bands were significantly depleted with the secondary protein structure shifted to a more β-pleated secondary protein one. CONCLUSION:Both sesamol and kojic acid display a similar pattern of antimelanogenesis activity albeit to a different degree. The mechanism of their whitening effect may be via the alteration of (a) the enzyme conformation disallowing the ordinary enzyme-substrate interaction and maybe (b) the integrity of the lipid-containing melanosome. Our results support the alternative use of FTIR microspectroscopy as a simple and reagent-free method for characterization of biomolecular changes in human melanoma cells.

journal_name

J Dermatol Sci

journal_title

Journal of dermatological science

authors

Srisayam M,Weerapreeyakul N,Barusrux S,Tanthanuch W,Thumanu K

doi

10.1016/j.jdermsci.2013.11.002

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

241-50

issue

3

eissn

0923-1811

issn

1873-569X

pii

S0923-1811(13)00360-5

journal_volume

73

pub_type

杂志文章

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