Influences of keratinocyte-fibroblast interaction on the expression of epimorphin by fibroblasts in vitro.

Abstract:

:Epimorphin was demonstrated to be a mesenchymal signal factor modulating epithelial morphogenesis of skin, lung and liver in vitro. Most of the previous studies were performed biochemically and functionally. In the present study, expression of epimorphin was immunohistochemically compared between cultured fibroblasts and cocultured fibroblasts with keratinocytes obtained from normal skin. Cultured fibroblasts revealed a low level of epimorphin expression. In contrast, the expression by fibroblasts was greatly enhanced in skin explant culture where both fibroblasts and keratinocytes were present. In three-dimensional coculture of fibroblasts and keratinocytes, the expression of epimorphin was enhanced. The staining pattern of epimorphin in three-dimensional coculture was similar to that in human skin. These results suggest that dermal fibroblasts are manufacturers of epimorphin, and keratinocyte-fibroblast interaction may play important roles in the expression of epimorphin in vitro.

journal_name

J Dermatol Sci

authors

Zhang L,Ishikawa O,Takeuchi Y,Yokoyama Y,Miyachi Y

doi

10.1016/s0923-1811(98)00081-4

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

191-6

issue

3

eissn

0923-1811

issn

1873-569X

pii

S0923181198000814

journal_volume

20

pub_type

杂志文章
  • Immunoelectron microscopic analysis of lysosomal deposits in alpha-N-acetylgalactosaminidase deficiency with angiokeratoma corporis diffusum.

    abstract::Alpha-N-acetylgalactosaminidase (alpha-NAGA) deficiency with angiokeratoma corporis diffusum (AKCD) is one of the lysosomal storage diseases. GalNAc(alpha))1-O-Ser/Thr (Tn) is theoretically deposited in lysosomes, but substances with attached galactose and neuraminic (sialic) acid (T and sialosyl Tn, respectively) are...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/s0923-1811(02)00005-1

    authors: Kanda A,Tsuyama S,Murata F,Kodama K,Hirabayashi Y,Kanzaki T

    更新日期:2002-05-01 00:00:00

  • Gene silencing of extracellular matrix protein 1 (ECM1) results in phenotypic alterations of dermal fibroblasts reminiscent of clinical features of lichen sclerosus.

    abstract:BACKGROUND:Lichen sclerosus (LS) is an acquired inflammatory mucocutaneous disease affecting the anogenital area, characterized histologically by hyalinosis and thickened vessel walls in the dermis. The presence of serum autoantibodies against extracellular matrix protein 1 (ECM1) in LS patients may suggest its involve...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2020.06.010

    authors: Utsunomiya N,Utsunomiya A,Chino T,Hasegawa M,Oyama N

    更新日期:2020-11-01 00:00:00

  • Objective scratch monitor evaluation of the effect of an antihistamine on nocturnal scratching in atopic dermatitis.

    abstract::We previously reported a simple and easy-to-use device, Scratch Monitor for evaluation of nocturnal scratching. In the present study, the effect of an antihistamine (azelastine hydrochloride) on atopic dermatitis was investigated using this device. In 40 patients with atopic dermatitis, nocturnal scratching was measur...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/s0923-1811(99)00048-1

    authors: Endo K,Sano H,Fukuzumi T,Adachi J,Aoki T

    更新日期:1999-12-01 00:00:00

  • Unfolded protein response in keratinocytes: impact on normal and abnormal keratinization.

    abstract::The unfolded protein response (UPR) is a signaling pathway from the endoplasmic reticulum (ER) to the nucleus that protects cells from stress caused by misfolded or unfolded proteins. As such, ER stress is an ongoing challenge for all cells, given the central biologic importance of secretion as part of normal physiolo...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审

    doi:10.1016/j.jdermsci.2012.12.002

    authors: Sugiura K

    更新日期:2013-03-01 00:00:00

  • PCR and PCR-RFLP techniques targeting the DNA topoisomerase II gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis.

    abstract:BACKGROUND:We have focused on the DNA topoisomerase II genes of several pathogenic fungi, and developed polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) methods targeting this gene for identification of dermatophytes. OBJECTIVE:To assess the availability of the PCR-based identifi...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2003.10.007

    authors: Kamiya A,Kikuchi A,Tomita Y,Kanbe T

    更新日期:2004-02-01 00:00:00

  • Expression of antimicrobial peptides in diabetic foot ulcer.

    abstract:BACKGROUND:Foot ulcers are one of the main diabetes complications due to its high frequency and difficulty of complete healing. There are several factors that participate in diabetic ulcers development and limited information exists about the role of antimicrobial peptides (AMP) in its pathogenesis. OBJECTIVE:The aim ...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2011.09.013

    authors: Rivas-Santiago B,Trujillo V,Montoya A,Gonzalez-Curiel I,Castañeda-Delgado J,Cardenas A,Rincon K,Hernandez ML,Hernández-Pando R

    更新日期:2012-01-01 00:00:00

  • Alterations of immune functions in barrier disrupted skin by UVB irradiation.

    abstract:BACKGROUND:While immunologic events elicited by acute barrier disruption or UVB irradiation have been studied in detail, the biological sequel of multiple insults to the skin is not well understood. OBJECTIVE:Since the skin would receive a variety of simultaneous stimuli in daily life, we tested the effects of sequent...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/s0923-1811(03)00177-4

    authors: Ito T,Seo N,Yagita H,Tsujimura K,Takigawa M,Tokura Y

    更新日期:2003-12-01 00:00:00

  • Neutrophil elastase contributes to extracellular matrix damage induced by chronic low-dose UV irradiation in a hairless mouse photoaging model.

    abstract:BACKGROUND:Repeated exposure to ultraviolet (UV) rays damages skin connective tissue, which is thought to be associated with wrinkle formation. We hypothesized that repeated mild inflammation may cause the connective tissue alterations in photoaging. OBJECTIVE:To clarify the behavior of neutrophils and neutrophil elas...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2010.09.001

    authors: Takeuchi H,Gomi T,Shishido M,Watanabe H,Suenobu N

    更新日期:2010-12-01 00:00:00

  • Autoimmune reactivity against precursor form of desmoglein 1 in healthy Tunisians in the area of endemic pemphigus foliaceus.

    abstract:BACKGROUND:Desmoglein 1 (Dsg1), the pemphigus foliaceus (PF) antigen, is produced as a precursor (preDsg1) and is transported to the cell surface as the mature form (matDsg1). Recent studies show that B cells from North American individuals without pemphigus can potentially produce anti-preDsg1 IgG antibodies, but ELIS...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2013.02.002

    authors: Toumi A,Saleh MA,Yamagami J,Abida O,Kallel M,Masmoudi A,Makni S,Turki H,Hachiya T,Kuroda K,Stanley JR,Masmoudi H,Amagai M

    更新日期:2013-04-01 00:00:00

  • Neither cyclosporin A nor FK506 affects adenylate cyclase responses of fetal rat keratinizing epidermal cells (FRSK cells) at concentrations that inhibit thymidine incorporation.

    abstract::It has been reported that various anti-psoriatic agents augment the beta-adrenergic adenylate cyclase response that is defective in the psoriatic hyperproliferative epidermis. Recent evidence indicates that cyclosporin A (CsA) and FK506 show beneficial effects on psoriasis. Using fetal rat keratinizing epidermal cells...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/0923-1811(93)90044-p

    authors: Takahashi H,Iizuka H

    更新日期:1993-12-01 00:00:00

  • Cultured human dermal papilla cells secrete a chemotactic factor for melanocytes.

    abstract::Large numbers of pigmented melanocytes are located in human hair follicles, predominantly around the dermal papillae, and the number of melanocytes and the melanogenic activity of the hair follicles are closely related to the hair cycle. We found that cultured human dermal papilla cells secreted a melanocyte chemoattr...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/s0923-1811(01)00145-1

    authors: Ideta R,Soma T,Tsunenaga M,Ifuku O

    更新日期:2002-01-01 00:00:00

  • Topically applied Melaleuca alternifolia (tea tree) oil causes direct anti-cancer cytotoxicity in subcutaneous tumour bearing mice.

    abstract:BACKGROUND:Melaleuca alternifolia (tea tree) oil (TTO) applied topically in a dilute (10%) dimethyl sulphoxide (DMSO) formulation exerts a rapid anti-cancer effect after a short treatment protocol. Tumour clearance is associated with skin irritation mediated by neutrophils which quickly and completely resolves upon tre...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2012.05.005

    authors: Ireland DJ,Greay SJ,Hooper CM,Kissick HT,Filion P,Riley TV,Beilharz MW

    更新日期:2012-08-01 00:00:00

  • Ligand-activated PPARδ upregulates α-smooth muscle actin expression in human dermal fibroblasts: A potential role for PPARδ in wound healing.

    abstract:BACKGROUND:The phenotypic changes that accompany differentiation of resident fibroblasts into myofibroblasts are important aspects of the wound healing process. Recent studies showed that peroxisome proliferator-activated receptor (PPAR) δ plays a critical role in wound healing. OBJECTIVE:To determine whether the nucl...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2015.10.005

    authors: Ham SA,Hwang JS,Yoo T,Lee WJ,Paek KS,Oh JW,Park CK,Kim JH,Do JT,Kim JH,Seo HG

    更新日期:2015-12-01 00:00:00

  • Localization of fibronectin in the initial lymphatics and blood vessels of normal human skin: an immunoelectron microscopic investigation.

    abstract::The initial lymphatics of the human skin do not possess a basal lamina, but a subendothelial fibrous network, of which we do not know the biochemical and immunohistological composition. We investigated the wall of blood vessels and initial lymphatics in normal human skin for the presence of fibronectin by immunoelectr...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/0923-1811(92)90060-o

    authors: Lubach D,Nissen S,Marghescu S

    更新日期:1992-09-01 00:00:00

  • Novel ex vivo disease model for extramammary Paget's disease using the cancer tissue-originated spheroid method.

    abstract:BACKGROUND:Extramammary Paget's disease (EMPD) is a rare skin cancer that frequently occurs in the anogenital region in the elderly. Prognosis in patients with metastatic EMPD is poor as EMPD treatment has advanced little in recent years, primarily because no EMPD cell line has been established. OBJECTIVE:We aimed to ...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2020.07.006

    authors: Arita T,Kondo J,Kaneko Y,Tsutsumi M,Kanemaru M,Matsui M,Arakawa Y,Katoh N,Inoue M,Asai J

    更新日期:2020-09-01 00:00:00

  • Epidermal basement membrane zone components: ultrastructural distribution and molecular interactions.

    abstract::The epidermal basement membrane zone (BMZ) comprises a multiprotein complex that aids the attachment of epidermal keratinocytes to the underlying dermis. In the last 5 years, our understanding of epidermal BMZ morphology, structure and function has dramatically improved. A complex network of molecular interactions has...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审

    doi:10.1016/s0923-1811(03)00045-8

    authors: McMillan JR,Akiyama M,Shimizu H

    更新日期:2003-05-01 00:00:00

  • A proinflammatory role of KLK6 protease in Netherton syndrome.

    abstract:BACKGROUND:Netherton syndrome (NS) is a rare but severe type of ichthyosis characterized by atopy, allergies, and potentially lethal skin overdesquamation associated with highly elevated proteolytic activities in LEKTI-deficient epidermis. NS symptoms are recapitulated in Spink5-/- mouse where the gene encoding Lekti h...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2019.06.004

    authors: Zingkou E,Pampalakis G,Charla E,Nauroy P,Kiritsi D,Sotiropoulou G

    更新日期:2019-07-01 00:00:00

  • Superantigen-induced cytokine expression in organ-cultured human skin.

    abstract::Bacterial superantigen such as staphylococcal enterotoxin B (SEB) induced strong ICAM-1 expression in organ-cultured human keratinocytes. Other superantigens (SEA, SEC1, SEC2) but not mite antigen (Dermatophagoides) also induced ICAM-1 expression both at protein and mRNA level. In contrast to ICAM-1, vascular endothel...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/0923-1811(95)00426-2

    authors: Matsunaga T,Katayama I,Yokozeki H,Nishioka K

    更新日期:1996-02-01 00:00:00

  • Genetic variation at IL12B, IL23R and IL23A is associated with psoriasis severity, psoriatic arthritis and type 2 diabetes mellitus.

    abstract:BACKGROUND:Common DNA variants in IL12B, IL23R and IL23A have been associated with an increased susceptibility to psoriasis (Ps) and psoriatic arthritis (PsA). Metabolic comorbidities and cardiovascular risk factors have also been associated to both Ps and PsA. OBJECTIVE:To analyze the effect of single nucleotide poly...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2014.05.010

    authors: Eirís N,González-Lara L,Santos-Juanes J,Queiro R,Coto E,Coto-Segura P

    更新日期:2014-09-01 00:00:00

  • Regulation of keratinocyte differentiation by O-GlcNAcylation.

    abstract:BACKGROUND:O-linked β-N-acetylglucosamine (O-GlcNAc) modification is one of the posttranslational modification, emerging as an important regulatory mechanism in various cellular events. OBJECTIVE:We attempted to investigate whether O-GlcNAcylation is involved in keratinocyte differentiation. METHODS:Immunohistochemis...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2014.04.010

    authors: Sohn KC,Lee EJ,Shin JM,Lim EH,No Y,Lee JY,Yoon TY,Lee YH,Im M,Lee Y,Seo YJ,Lee JH,Kim CD

    更新日期:2014-07-01 00:00:00

  • Adherence characteristics and susceptibility to antimicrobial agents of Staphylococcus aureus strains isolated from skin infections and atopic dermatitis.

    abstract::We examined the adherence characteristics and susceptibility to various antimicrobial agents of 130 strains of Staphylococcus aureus isolated from infective skin lesions and 135 strains of S. aureus isolated from non-infective eczematous lesions of atopic dermatitis (AD) patients. The isolation rate of methicillin-res...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/s0923-1811(00)00070-0

    authors: Akiyama H,Yamasaki O,Tada J,Arata J

    更新日期:2000-08-01 00:00:00

  • Dermal carotenoid level and kinetics after topical and systemic administration of antioxidants: enrichment strategies in a controlled in vivo study.

    abstract:BACKGROUND:High doses of sun-emitted UV-radiation induce reactive oxygen species (ROS) as major pro-oxidants thus inducing premature skin aging. The best prevention of the destructive action of free radicals in human skin is textile coverings, topical sunscreens and the development of a high antioxidative protective ne...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2011.06.009

    authors: Darvin ME,Fluhr JW,Schanzer S,Richter H,Patzelt A,Meinke MC,Zastrow L,Golz K,Doucet O,Sterry W,Lademann J

    更新日期:2011-10-01 00:00:00

  • Hydroquinone-salicylic acid conjugates as novel anti-melasma actives show superior skin targeting compared to the parent drugs.

    abstract:BACKGROUND:Hydroquinone (HQ) and salicylic acid (SA) are drugs for treating melasma through the mechanisms of tyrosinase inhibition and chemical peeling, respectively. Their high frequency of causing skin irritation has led to limited use of both drugs. OBJECTIVES:We designed the new conjugates obtained by joining HQ ...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2014.08.013

    authors: Hsieh PW,Aljuffali IA,Fang CL,Chang SH,Fang JY

    更新日期:2014-11-01 00:00:00

  • Malignant blue nevus: a report of eight cases.

    abstract::Malignant blue nevus is uncommon compared to its benign counterpart and is regarded as a rare form of malignant melanoma. We report the clinical and histological findings in eight cases. Histologically, all eight specimens showed no epidermal involvement and had contained within or were adjacent to portions of blue ne...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/0923-1811(92)90018-7

    authors: Mehregan DA,Gibson LE,Mehregan AH

    更新日期:1992-11-01 00:00:00

  • IL-11, IL-1α, IL-6, and TNF-α are induced by solar radiation in vitro and may be involved in facial subcutaneous fat loss in vivo.

    abstract:BACKGROUND:The loss of subcutaneous (sc) fat is associated with aging. Inflammatory cytokines, such as interleukin-1 α (IL-1α), interleukin-11 (IL-11) and tumor necrosis factor-α (TNF-α), are known to inhibit the differentiation of preadipocytes. OBJECTIVE:This study investigated the potential role of inflammatory cyt...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2013.03.009

    authors: Li WH,Pappas A,Zhang L,Ruvolo E,Cavender D

    更新日期:2013-07-01 00:00:00

  • Therapeutic effects of streptococcal preparation OK-432 on atopic dermatitis-like lesions in NC/Nga mice: possible shift from a Th2- to Th1-predominance.

    abstract:BACKGROUND:The inducement of Th1 cell-mediated immune response, possibly brought about through bacterial stimulation, may serve to control atopic disorders such as atopic dermatitis (AD). The streptococcal preparation, OK-432, has been shown a potent Th1 inducer through the action of IL-12. NC/Nga mice under ordinary c...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2004.06.006

    authors: Horiuchi Y,Bae S,Katayama I,Nishioka K

    更新日期:2004-09-01 00:00:00

  • TGF-beta-induced SMAD signaling and gene regulation: consequences for extracellular matrix remodeling and wound healing.

    abstract::Members of the transforming growth factor-beta (TGF-beta) superfamily are pleiotropic cytokines that have the ability to regulate numerous cell functions, including proliferation, differentiation, apoptosis, epithelial-mesenchymal transition, and production of extracellular matrix, allowing them to play an important r...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章,评审

    doi:10.1016/j.jdermsci.2003.12.006

    authors: Schiller M,Javelaud D,Mauviel A

    更新日期:2004-08-01 00:00:00

  • Tight junction dysfunction in the stratum granulosum leads to aberrant stratum corneum barrier function in claudin-1-deficient mice.

    abstract:BACKGROUND:Tight junctions (TJs) contribute to the epithelial barrier function by preventing leakage of solutes through the intercellular space. In the skin, TJs occur in the stratum granulosum (SG), where claudin-1 and claudin-4 are expressed as adhesion molecules of TJs. Claudin-1-deficient (Cldn1(-/-)) mice die with...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2013.01.002

    authors: Sugawara T,Iwamoto N,Akashi M,Kojima T,Hisatsune J,Sugai M,Furuse M

    更新日期:2013-04-01 00:00:00

  • Serum squamous cell carcinoma antigen (SCCA)-2 correlates with clinical severity of pediatric atopic dermatitis in Ishigaki cohort.

    abstract:BACKGROUND:We sometimes encounter difficulties in assessing the severity of pediatric atopic dermatitis (AD) using currently available biomarkers such as thymus and activation-regulated chemokine (TARC) due to the higher baseline values in non-AD children. Recent case control studies have indicated the usefulness of sq...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2019.07.005

    authors: Takeuchi S,Furusyo N,Ono J,Azuma Y,Takemura M,Esaki H,Yamamura K,Mitamura Y,Tsuji G,Kiyomatsu-Oda M,Hayashi J,Izuhara K,Furue M

    更新日期:2019-08-01 00:00:00

  • Keratinocytes in atopic dermatitis express abundant ΔNp73 regulating thymic stromal lymphopoietin production via NF-κB.

    abstract:BACKGROUND:Atopic dermatitis (AD) is a chronic inflammatory skin disease that often cannot be completely controlled by modern medicine. Since multiple factors are intricately involved in the pathogenesis of AD, wide-ranging research is required for further advancement of AD treatment. Epidermal keratinocytes are the fo...

    journal_title:Journal of dermatological science

    pub_type: 杂志文章

    doi:10.1016/j.jdermsci.2017.06.017

    authors: Kumagai A,Kubo T,Kawata K,Kamekura R,Yamashita K,Jitsukawa S,Nagaya T,Sumikawa Y,Himi T,Yamashita T,Ichimiya S

    更新日期:2017-11-01 00:00:00