当前位置： SCI文献检索 > BRAIN RESEARCH期刊下所有文献 > gamma-Endorphin and schizophrenia: amino acid composition of gamma-endorphin and nucleotide sequence of gamma-endorphin cDNA from pituitary glands of schizophrenic patients.

gamma-Endorphin and schizophrenia: amino acid composition of gamma-endorphin and nucleotide sequence of gamma-endorphin cDNA from pituitary glands of schizophrenic patients.

Abstract：

:The possibility has been mentioned that a change in the structure is responsible for the deviant behavioral activity of gamma-endorphin in extracts of postmortem brain and pituitary gland samples of schizophrenic patients. This paper describes the investigation of this possibility by means of: amino acid composition analysis of alpha- and gamma-endorphin isolated from a pituitary gland of a schizophrenic patient; and nucleotide sequence analysis of the gamma-endorphin coding region of pro-opiomelanocortin (POMC) mRNA from two other pituitary glands, using the primer extension method. Both methods require no more than a single pituitary to obtain reliable results. alpha- and gamma-endorphin were isolated from an acid extract by gel filtration and two subsequent HPLC steps. In addition, the gamma-endorphin region of beta-endorphin was analyzed by enzymatic cleavage of beta-endorphin and isolation of the resulting fragment. Single-stranded gamma-endorphin cDNA was synthesized by reverse transcriptase using total cellular pituitary RNA and a 5' 32P-labeled oligodeoxyribonucleotide primer (20-mer) hybridizing close to the gamma-endorphin coding region of POMC mRNA. Single-stranded cDNA was digested with restriction enzyme HaeIII which generated a 148 nucleotides long radioactive cDNA fragment containing the gamma-endorphin cDNA sequence. The sequence of the 148 nucleotides fragment was determined. Neither the amino acid composition analysis nor the amino acid sequence derived from the cDNA nucleotide sequence revealed differences between schizophrenics and controls. Thus, no evidence was found for changes in the amino acid sequence of pituitary gamma-endorphin in these analyses, which include 3 cases of schizophrenia.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Brain Res

journal_title

Brain research

authors

Bovenberg RA,Burbach JP,Wiegant VM,Veeneman GH,van Boom JH,Baas PD,Jansz HS,de Wied D

doi

10.1016/0006-8993(86)90896-6

subject

Has Abstract

pub_date

1986-06-18 00:00:00

pages

29-37

issue

1

eissn

0006-8993

issn

1872-6240

pii

0006-8993(86)90896-6

journal_volume

376

pub_type

杂志文章

相关文献

BRAIN RESEARCH文献大全
  • Peroxisome proliferator-activated receptor gamma agonist rosiglitazone attenuates oxyhemoglobin-induced Toll-like receptor 4 expression in vascular smooth muscle cells.

    abstract：:Inflammation and immune response have been implicated in the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage (SAH). Recently, increased TLR4 expression has been associated with the development of cerebral vasospasm in a rabbit model of SAH. Peroxisome proliferator-activated receptor gamma (PPARgamma) ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2010.01.073

    authors： Wu Y,Zhao XD,Zhuang Z,Xue YJ,Cheng HL,Yin HX,Shi JX

    更新日期：2010-03-31 00:00:00

  • The role of altered sodium currents in action potential abnormalities of cultured dorsal root ganglion neurons from trisomy 21 (Down syndrome) human fetuses.

    abstract：:Trisomy 21 (Down syndrome) results in abnormalities in electrical membrane properties of cultured human fetal dorsal root ganglion (DRG) neurons. Action potentials have faster rates of depolarization and repolarization, with decreased spike duration, compared to diploid neurons. In order to analyze the faster depolari...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(90)91372-n

    authors： Caviedes P,Ault B,Rapoport SI

    更新日期：1990-03-05 00:00:00

  • Non-linear kinetics of glutamyl-tRNA synthesis catalyzed by high molecular weight complexes from rat brain neuronal cells but not from glial cells.

    abstract：:High molecular weight complexes of aminoacyl-tRNA synthetases isolated from rat brain catalyze the formation of glutamyl-tRNA with an initial lag time of the order of 1 min, as previously reported for the formation of glutamyl-tRNA and glutaminyl-tRNA catalyzed by similar complexes from bovine brain (Vadeboncoeur and ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0169-328x(86)90016-1

    authors： Lacoste L,Hertz L,Lapointe J

    更新日期：1986-07-01 00:00:00

  • Epidural motor cortex stimulation suppresses somatosensory evoked potentials in the primary somatosensory cortex of the rat.

    abstract：:Motor cortex stimulation (MCS) is a promising clinical procedure to help alleviate chronic pain. Animal models demonstrated that MCS is effective in lessening nocifensive behaviors. The present study explored the effects of MCS on cortical somatosensory evoked potentials (SEPs) recorded at the primary somatosensory co...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2012.04.027

    authors： Chiou RJ,Lee HY,Chang CW,Lin KH,Kuo CC

    更新日期：2012-06-29 00:00:00

  • The mitochondrial division inhibitor mdivi-1 attenuates spinal cord ischemia-reperfusion injury both in vitro and in vivo: Involvement of BK channels.

    abstract：:Mitochondrial division inhibitor (mdivi-1), a selective inhibitor of a mitochondrial fission protein dynamin-related protein 1 (Drp1), has been shown to exert protective effects in heart and cerebral ischemia-reperfusion models. The present study was designed to investigate the beneficial effects of mdivi-1 against sp...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2015.03.033

    authors： Liu JM,Yi Z,Liu SZ,Chang JH,Dang XB,Li QY,Zhang YL

    更新日期：2015-09-04 00:00:00

  • Sexual dimorphism in the trigeminal motor neurons innervating the mouse masseter muscle.

    abstract：:The extrafusal and intrafusal muscle fibers in the masseter muscle are innervated by motoneurons in the trigeminal motor nucleus (Mo 5). In the present study, we found that the number of trigeminal motor neurons in the male mouse was significantly larger than that in the female. However, we could not detect any signif...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(93)90763-d

    authors： Maeda N,Sugiyama H,Suemune S,Wakisaka H,Niida S,Ogata K,Miyata K

    更新日期：1993-11-05 00:00:00

  • ETA and ETB receptor antagonists synergistically increase extracellular endothelin-1 levels in primary rat astrocyte cultures.

    abstract：:Astrocytes produce and bind endothelins (ETs), suggesting that these cells have ET autoregulatory and eliminatory functions. To further investigate these functions in primary rat astrocytes, ET-1 levels in the cell culture media (RIA/HPLC) and intracellular content of ET-1 mRNA (RT PCR) were measured under basal and s...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(97)01368-1

    authors： Hasselblatt M,Kamrowski-Kruck H,Jensen N,Schilling L,Kratzin H,Sirén AL,Ehrenreich H

    更新日期：1998-03-02 00:00:00

  • Inositol 1,4,5-trisphosphate 3-kinase distribution in the rat brain. High levels in the hippocampal CA1 pyramidal and cerebellar Purkinje cells suggest its involvement in some memory processes.

    abstract：:The distribution of inositol 1,4,5-trisphosphate (InsP3) 3-kinase was studied in the adult rat brain, using polyclonal antibodies raised against the purified 50,000-Da rat brain enzyme by immunohistochemistry and Western blot, in addition to enzymatic assay. Immunohistochemically, the enzyme was detected in neurons, w...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(91)91622-8

    authors： Mailleux P,Takazawa K,Erneux C,Vanderhaeghen JJ

    更新日期：1991-01-25 00:00:00

  • Stimulation-induced status epilepticus: role of the hippocampal mossy fibers in the seizures and associated neuropathology.

    abstract：:A study of seizure activity and neuronal cell death produced by intracerebroventricular kainic acid had suggested that seizures conveyed by the hippocampal mossy fibers are more damaging to CA3 pyramidal cells than seizures conveyed by other pathways. To test this idea, the effects of a unilateral mossy fiber lesion w...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(90)91171-c

    authors： Vicedomini JP,Nadler JV

    更新日期：1990-03-26 00:00:00

  • The effects of acute and chronic morphine treatment on the process of facial nerve regeneration.

    abstract：:Prominent cellular responses to axonal interruption include enhanced synthesis of RNA and protein in the neuronal perikaryon, and proliferation of reactive Schwann cells. Since morphine has been shown to significantly depress cellular metabolism, we examined its effect on these and other reparative responses underlyin...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(79)91010-2

    authors： Sinatra RS,Ford DH

    更新日期：1979-10-19 00:00:00

  • Insulin inhibits specific norepinephrine uptake in neuronal cultures from rat brain.

    abstract：:Neuronal cells in primary culture have been demonstrated to possess specific insulin receptors (Boyd et al., J. Biol. Chem., 260 (1985) 15880-15884). Incubation of these cultures with insulin causes a dose-dependent inhibition of maprotiline-sensitive [3H]norepinephrine uptake. Maximum inhibition of 95% of maprotiline...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(86)91242-4

    authors： Boyd FT Jr,Clarke DW,Raizada MK

    更新日期：1986-11-19 00:00:00

  • Purkinje cell loss from alternating sagittal zones in the cerebellum of leaner mutant mice.

    abstract：:The cerebellum and inferior olive of adult homozygous leaner mutant mice (tgIa/tgIa) were examined using anti-vitamin D-dependent calcium binding protein immunohistochemistry, and Nissl staining. The immunohistochemical staining revealed that cerebellar Purkinje cell loss in leaner is restricted to alternating saggita...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(09)90014-2

    authors： Heckroth JA,Abbott LC

    更新日期：1994-09-26 00:00:00

  • Presynaptic adenosine A2a receptors on soma and central terminals of rat vagal afferent neurons.

    abstract：:The dorsal vagal complex of the medulla oblongata is a key centre involved in the regulation of numerous autonomic functions, including cardiovascular control. Adenosine has been implicated as a potential neuromodulator of the baroreceptor reflex, and therefore the current study has investigated the presence and chara...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(94)90327-1

    authors： Castillo-Meléndez M,Krstew E,Lawrence AJ,Jarrott B

    更新日期：1994-07-25 00:00:00

  • Purification and mass spectrometric analysis of the kappa opioid receptor.

    abstract：:A clonal human embryonic kidney (HEK) 293 cell line was established that stably expressed the rat kappa-opioid receptor (rKOR) with a FLAG epitope at the amino terminus. The Kd for [3H]diprenorphine was 1.1+/-0.2 nM, and the Bmax was 2.6+/-0.4 pmol/mg. Dynorphin A (1-13), U69,593 and naloxone competitively inhibited [...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2008.06.121

    authors： Wannemacher KM,Terskiy A,Bian S,Yadav PN,Li H,Howells RD

    更新日期：2008-09-16 00:00:00

  • Ethanol-induced state-dependent learning is mediated by 5-hydroxytryptamine3 receptors but not by N-methyl-D-aspartate receptor complex.

    abstract：:Involvement of N-methyl-D-aspartate (NMDA) receptor complex and 5-hydroxytryptamine3 (5-HT3) receptors in state-dependent learning (SDL) induced by ethanol (EtOH) was investigated in the step-through passive avoidance task in rats. Pre-training injections of EtOH or MK-801 reduced step-through latency in the test sess...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(95)01040-8

    authors： Nakagawa Y,Iwasaki T

    更新日期：1996-01-15 00:00:00

  • Regeneration of gamma-fusimotor axons after nerve-freeze injury in the cat.

    abstract：:Nerve injury was produced by application of dry ice to the common peroneal nerve. After 20 weeks, muscle-spindle primary endings responded normally to stretch and the spindles were found to have a fully functional fusimotor innervation. ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(85)90372-5

    authors： Scott JJ

    更新日期：1985-11-25 00:00:00

  • Lesions of the inferior olivary complex cause extinction of the classically conditioned eyeblink response.

    abstract：:The dentate-interpositus nuclei of the cerebellum are known to be critically involved in the production of the classically conditioned eyeblink response in the rabbit. The rostro-medial portions of the inferior olivary complex (rmIO) project to these nuclei as well as receive projections from the fifth sensory nuclei....

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(85)91419-2

    authors： McCormick DA,Steinmetz JE,Thompson RF

    更新日期：1985-12-16 00:00:00

  • Postnatal development of the endothelial system of the rat cerebellum using immunohistochemical techniques.

    abstract：:The development of the endothelial system of the rat cerebellum was studied by immunohistochemical techniques with two antibodies specific of two membrane antigens characteristic of 'mature' endothelial cells. It appears that the maturation of endothelial cells is very late in the rat cerebellum. This absence of a ful...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0165-3806(85)90149-x

    authors： Zanetta JP,Dontenwill M,Bricca G

    更新日期：1985-01-01 00:00:00

  • The effects of stimulation and lesion of raphe nuclei on luteinizing hormone release in estrogen-progesterone-treated ovariectomized rats.

    abstract：:A study was made of the effects of electrochemically stimulating (ECS) and destroying the midbrain dorsal and median raphe nuclei on the estrogen-progesterone- (EP) induced surge of pituitary LH release in ovariectomized rats. ECS and lesions were produced simultaneously with anodal direct current through a chronicall...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(81)90006-8

    authors： Waloch M,Gilman D,Whitmoyer D,Sawyer CH

    更新日期：1981-08-03 00:00:00

  • Expression of metallothionein-III induced by hypoxia attenuates hypoxia-induced cell death in vitro.

    abstract：:Metallothioneins (MTs) are metal-binding proteins that are expressed in many tissues including brain. MTs protect cells and organs against metal toxicity and oxidants. Among MTs, a brain-predominant subtype MT-III has prominent neuroprotective activity against various types of damage. Here we show that the expression ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(03)02633-7

    authors： Tanji K,Irie Y,Uchida Y,Mori F,Satoh K,Mizushima Y,Wakabayashi K

    更新日期：2003-06-20 00:00:00

  • Nuclear RNA metabolism in rat hippocampal slices incubated in vitro.

    abstract：:Rat hippocampal slices were incubated with [3H]uridine in vitro to analyze the metabolism of nuclear RNA and the RNA precursor fractions. Labeling of total nuclear RNA was linear for 4 h of incubation and proportional to the concentration of labeled uridine in the incubation medium. Addition of 3.5 X 10(-8) M corticos...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(84)90375-5

    authors： Dokas LA,Liauw A

    更新日期：1984-07-23 00:00:00

  • The derivation and characterization of neuronal cell lines from rat and mouse brain.

    abstract：:This study shows that permanent cell lines can be established from rat and mouse brain by direct tissue culture methodology without the aid of exogenous chemical or viral transforming agents. These cells were derived from specific areas of the brain, such as the cerebellum and hippocampus, at chosen times during fetal...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(77)91049-6

    authors： Bulloch K,Stallcup WB,Cohn M

    更新日期：1977-10-21 00:00:00

  • Selective loss of subpopulations of ventral mesencephalic dopaminergic neurons in the monkey following exposure to MPTP.

    abstract：:Tyrosine hydroxylase immunohistochemical examination of the mesencephalon of severely parkinsonian MPTP-treated macaque fascicularis monkeys revealed a marked loss of substantia nigra pars compacta (SNc) neurons in both medial and central portions of the nucleus with a relative sparing of neurons in the dorsal-most po...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(87)90691-3

    authors： Schneider JS,Yuwiler A,Markham CH

    更新日期：1987-05-12 00:00:00

  • Expression of only one myelin basic protein allele in mouse is compatible with normal myelination.

    abstract：:Myelin deficiency (mld) is an autosomal recessive mutation in mice characterized by a severe myelin deficit in the central nervous system (CNS). The primary defect in mld is a reduction of the synthesis of the myelin basic protein (MBP) and probably lies in a regulatory element of the MBP gene. In young mld heterozygo...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0169-328x(87)90045-3

    authors： Roch JM,Cooper BJ,Ramirez M,Matthieu JM

    更新日期：1987-12-01 00:00:00

  • Involvement of medial septal glutamate and GABAA receptors in behaviour-induced acetylcholine release in the hippocampus: a dual probe microdialysis study.

    abstract：:In the present study, the role of medial septal receptors in behaviour-induced increase in acetylcholine (ACh) release in hippocampus was investigated using dual-probe microdialysis in combination with a simple behavioural procedure. gamma-Aminobutyric acid (GABA) and glutamate receptor agonists and antagonists were a...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(97)01445-5

    authors： Moor E,Schirm E,Jacsó J,Westerink BH

    更新日期：1998-04-06 00:00:00

  • Synaptic connections between pars compacta and pars reticulata neurones: electrophysiological evidence for functional modules within the substantia nigra.

    abstract：:Intracellular recordings were performed in vitro from both pars compacta and pars reticulata neurones of the substantia nigra, and their postsynaptic responses to electrical stimulation within the nucleus were analysed. Intracellular staining by biocytin was used to reveal the morphology and location of the recorded n...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(94)91292-0

    authors： Hajós M,Greenfield SA

    更新日期：1994-10-17 00:00:00

  • The site of the anti-emetic action of tachykinin NK1 receptor antagonists may exist in the medullary area adjacent to the semicompact part of the nucleus ambiguus.

    abstract：:NK1 receptor antagonists have been shown to act centrally and to produce a broad-spectrum anti-emetic action. To determine precisely the site of this action, we microinjected GR205171, an NK1 receptor antagonist, into the left medulla oblongata in decerebrate paralyzed dogs. The right medulla was transected 2.5 mm ros...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(98)01324-9

    authors： Fukuda H,Nakamura E,Koga T,Furukawa N,Shiroshita Y

    更新日期：1999-02-13 00:00:00

  • Effects of the 5-HT2A antagonist mirtazapine in rat models of thermoregulation.

    abstract：:Thermoregulation is a complex intercommunicative function requiring coordination between core body temperature (CBT), the central nervous system, and peripheral vasculature. In menopausal women, dysregulation of thermoregulatory mechanisms leads to hot flushes and night sweats. A previous study in ovariectomized (OVX)...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2006.09.050

    authors： Pawlyk AC,Cosmi S,Alfinito PD,Maswood N,Deecher DC

    更新日期：2006-12-06 00:00:00

  • Effects of lesions in the hypothalamic paraventricular, supraoptic and suprachiasmatic nuclei on vasopressin and oxytocin in rat brain and spinal cord.

    abstract：:The content of arginine vasopressin and oxytocin in various extrahypothalamic sites of the rat brain and spinal cord was determined by specific radioimmunoassays after lesions had been made in either the paraventricular (PVN), supraoptic (SON) or suprachiasmatic nuclei (SCN). In some animals all 3 nuclei were destroye...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(85)91093-5

    authors： Hawthorn J,Ang VT,Jenkins JS

    更新日期：1985-10-28 00:00:00

  • Intracellular plasma proteins in human fetal choroid plexus during development. II. The distribution of prealbumin, albumin, alpha-fetoprotein, transferrin, IgG, IgA, IgM, and alpha 1-antitrypsin.

    abstract：:Individual choroid plexuses of human fetal brain at different developmental stages were investigated by an indirect immunoperoxidase technique. The number and distribution of prealbumin-, albumin-, alpha-fetoprotein-, transferrin-, IgG-, IgA-, IgM- and alpha 1-antitrypsin-containing choroid plexus epithelial cells wer...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0165-3806(82)90025-6

    authors： Jacobsen M,Jacobsen GK,Clausen PP,Saunders NR,Møllgård K

    更新日期：1982-02-01 00:00:00