Abstract：
BACKGROUND/AIM:Ras-related associated with diabetes (RRAD), a member of the Ras-related GTPase superfamily, is frequently methylated in several human cancers, though its methylation profile remains unclear in esophageal cancer. MATERIALS AND METHODS:We examined RRAD promoter hypermethylation using real-time quantitative methylation-specific PCR in 229 primary human esophageal tissues of contrasting histological types. RESULTS:RRAD hypermethylation showed highly discriminative receiver-operator characteristic curve profiles, clearly distinguishing esophageal squamous cell carcinoma (ESCC) from esophageal adenocarcinoma (EAC) or normal esophagus (NE) (p<0.01 and p<0.01, respectively). RRAD normalized methylation values were significantly higher in ESCC (0.0242) than in NE (0.0057, p<0.05) or EAC (0.0139, p<0.01). RRAD hypermethylation frequency was also significantly higher in ESCC (23.1%) than in NE (0%, p<0.05) or EAC (5.4%, p<0.05). CONCLUSION:Promoter hypermethylation of RRAD is a frequent, tissue-specific event in ESCC, and is uncommon in EAC. The aberrant methylation of RRAD may be involved in the pathogenesis of a subset of ESCC, but not in EAC.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Jin Z,Feng X,Jian Q,Cheng Y,Gao Y,Zhang X,Wang L,Zhang Y,Huang W,Fan X,Chen S,Yu H,Zhao Z,Dong M,Liu J,Mori Y,Meltzer SJsubject
Has Abstractpub_date
2013-11-01 00:00:00pages
5199-203issue
11eissn
0250-7005issn
1791-7530pii
33/11/5199journal_volume
33pub_type
杂志文章abstract：AIM:Eudragit® E 100 (EE100) was used to improve the transfection efficiency of polyethylenimine (PEI). MATERIALS AND METHODS:Mobility of PEI-DNA complexes with and without EE100 were visualized by agarose gel electrophoresis and their transfection efficiencies were investigated in KB human oral carcinoma cells by flow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a cancer/testis antigen and predominant target for cancer immunotherapy. Its detection is only established based on gene expression. In this study, we established a monoclonal antibody against KK-LC-1 to detect its protein expression in formalin-fixed samples. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12937
更新日期：2018-10-01 00:00:00
abstract：PURPOSE:Investigation of the oxygenation status of organotypic multicellular spheroids (OMS) and their response to irradiation. MATERIALS AND METHODS:Tumour specimens of glioblastoma multiforme patients (n = 16) were initiated as OMS. Following 20 Gy gamma-irradiation, the cell migratory capacity was evaluated. Sphero...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：BACKGROUND/AIM:Intraperitoneal chemotherapy with taxanes provides high locoregional drug concentrations. Regarding their synergy with hyperthermia, results have been inconclusive. In this in vitro study, the thermal enhancement of the effect of paclitaxel and docetaxel on ovarian cancer cells under conditions mimicking...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14700
更新日期：2020-12-01 00:00:00
abstract：:Cells cultured as spheroids present an heterogeneity similar to that of tumours in vivo. In the spheroid peripheral layers, cells are proliferating, deeper cells are non-cycling, when in the aggregate centre, cells form often a necrotic core. A multicellular resistance is related on the cell contact to other cells or ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1998-11-01 00:00:00
abstract：BACKGROUND AND AIM:The clinical efficiency of cisplatin (CDDP) against gastric cancer is often limited by the development of resistance. A third-generation platinum-containing agent, oxaliplatin (L-OHP), has been introduced for treating gastric cancer. Here, we studied oxaliplatin in vitro to reveal the mechanism of ac...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:We used non-linear kernel discriminant analysis (KDA) to predict the outcome of 134 axillary node-negative primary breast cancer patients not treated with adjuvant therapy in a non censored database. MATERIAL:Posterior probabilities of relapse at 5 years were estimated using probabilistic neural networks (P...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:The effects of all-trans retinoic acid (ATRA), either alone or in combination with GM-CSF, on the induction of differentiation of a human myeloblastic leukemia cell line, ML-1, were investigated. ATRA alone caused only slight induction of NBT-reducing activity even at a high concentration (10(-7) M), but when combined...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:This article describes previous studies on a unique protein antitumor antibiotic, neocarzinostatin. Namely, its chemical nature, mode of action at molecular and cellular levels, toxicity and pharmacology, and recent results in clinical trials obtained primarily in Japan have been reviewed briefly. ...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,评审
doi：
更新日期：1981-01-01 00:00:00
abstract：BACKGROUND:Incidence of brain metastases (BM) from breast cancer (BC) is increasing. However, prognostic evaluation and treatment strategies are still a matter of debate. AIM:To describe the clinical outcome of BM from BC treated by neurosurgical resection and to identify the actual prognostic factors in this specific...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
abstract：AIM:The American thyroid association (ATA) recommends that additional imaging procedures supplement cervical ultrasonography (US) in any patient with a basal calcitonin value above 150 pg/ml in the follow-up of medullary thyroid carcinoma (MTC). The aim of the present study was to reaffirm or challenge this cut-off for...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：:Hyperthermia is effective for the treatment of cancer when applied concomitantly with chemotherapy and irradiation. However, it is difficult to heat deep portions of the body including the esophagus. Cancer of the esophagus still poses considerable treatment problems, with a poor 5-year survival rate after surgery, an...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,评审
doi：
更新日期：1992-05-01 00:00:00
abstract：:In order to estimate the environmental risk of the use of Alachlor, experiments on laboratory animals were conducted. Alachlor and 2,6 diethylaniline content in blood serum was quantified. Three groups of male ACI/T rats and C3H/FEJ mice were treated with three different doses of Alachlor. Six hours after the intraper...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:The aim of this study was to investigate whether multiple injections (daily for 5 days) of the hemorheological agent pentoxifylline (PTX) affected tumor physiological parameters (i.e., tumor pO2, tumor pH, and tumor interstitial fluid pressure) during the tumor growth of FSaII murine fibrosarcoma in C3H mice. The radi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:A pilot study to evaluate the predictive value of Indium-111-labelled somatostatin analogue [DTPA-D-Phe1]-octreotide scintigraphy (OctreoScan111) in the Octreotide treatment of hormone-refractory prostatic adenocarcinoma was initiated. Ten patients were investigated with OctreoScan111 with regard to disease extension ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The usefulness of lymphocyte subset change as an indicator for predicting survival time and the effectiveness of combined treatment with an immunopotentiator, lentinan, and carboplatin (CBDCA) were investigated. Of 13 patients with advanced unresectable cancer entered in this study, 9 were administered 2 mg/body lenti...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：BACKGROUND/AIM:This study assessed the toxicity and activity of biweekly docetaxel and S-1 combination therapy in elderly patients with advanced gastric cancer. PATIENTS AND METHODS:One-hundred and thirteen patients were enrolled: 35 were 75 years old or more. The objective response rate, toxicity, progression-free su...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2013-02-01 00:00:00
abstract：BACKGROUND/AIM:Extracts of Paeonia suffruticosa are traditionally used in Chinese medicine to increase blood flow. Recently, this extract has been shown to possess anti-tumor and anti-inflammatory properties, though this mechanism remains unknown. In the current work, we prepared extracts of P. suffruticosa and analyze...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11888
更新日期：2017-09-01 00:00:00
abstract：:Many arylamines (including carcinogens) and hydrazine drugs are acetylated by the cytosolic N-acetyltransferase (NAT). NAT plays an important role in the first step of arylamine metabolism and has been found in immortalized human cell lines, human and laboratory animal tissues. Human colon tumor cell line (colo 205) h...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:Vitamin D is a steroid hormone traditionally recognized for maintaining calcium and phosphorous homeostasis in the body. However, it is now widely accepted that it exerts several extraskeletal actions, including anti-tumorigenic and immunomodulatory effects in vitro and in vivo. There is now a huge interest in studyin...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2012-01-01 00:00:00
abstract：:This epidemiological study was performed to evaluate the influence of postoperative complementary treatment with lectin-standardized mistletoe extract (sME) on breast cancer patients. The design (retrolective cohort analysis with parallel groups) and conduct of the study were in agreement with current standards for pr...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：
更新日期：2003-11-01 00:00:00
abstract：AIM:The aim of this study was to evaluate the detection rate of bone metastases and the added value of 11C-acetate (ACE) positron-emission tomography/computed tomography (PET/CT) compared to bone scintigraphy (BS) in high-risk prostate cancer (PC). MATERIALS AND METHODS:A total of 66 untreated patients with high-risk ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11246
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND/AIM:The mechanism responsible for B-cell translocation gene 1 (BTG1) down-regulation in breast carcinoma remains unknown. We examined the BTG1 expression status in breast carcinoma cells and investigated the mechanism underlying the observed alterations. MATERIALS AND METHODS:Four breast carcinoma cell line...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13729
更新日期：2019-10-01 00:00:00
abstract：:The M-VAC regimen (methotrexate, vinblastine, doxorubicin and cisplatin) is widely used in the treatment of advanced or metastatic bladder cancer. In the present trial, an alternate week regimen of M-VEC (with epidoxorubicin instead of doxorubicin) supported by G-CSF (filgrastim) was evaluated. Eligible patients had m...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:This review focuses on the functional role and structural features of the genes involved in common hereditary cancers. Most of these tumors are sporadic and the genetic alterations responsible for their genesis take place over several cell generations; nevertheless, 5 to 10% of the human tumors are hereditary, with a ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2000-11-01 00:00:00
abstract：:Adenovirus (ADV)-mediated gene therapy with the thymidine kinase (TK) gene under control of the Rous sarcoma virus (RSV) promotor followed by the administration of acyclovir has been established in vitro for the treatment of ovarian cancer cells and has been used as the basis for intraperitoneal phase I clinical trial...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：BACKGROUND:Lysozyme is a natural antimicrobial enzyme that is up-regulated in inflammatory diseases of the gastrointestinal tract. Pathogenic microbes have recently been identified in the esophageal mucosa in patients with Barrett's esophagus (BE). Lysozyme expression was evaluated in biopsies from patients with BE. M...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
abstract：:The vascular endothelial growth factor (VEGF) and its receptor (Flt) protein expression was immunologically studied individually and in combination with the microvascular density (MVD) determined by the factor 8-related antigen (F8RA) and their prognostic value in 118 patients with a pulmonary adenocarcinoma. The pres...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Plastins are members of a family of actin-binding proteins which exhibit a tissue-specific expression pattern. L-plastin, which is specifically expressed in hematopoietic cell lineage, has been proposed to be involved in the control of cell adhesion and motility. This protein is also frequently expressed in cell lines...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:The incidence of breast cancer is increasing rapidly in Taiwan but is still low compared with western countries. The impact of body size factors in western countries inspired us to have a case-control study. A matched case-control study was done on 122 pairs of incident cases of female breast cancer and community cont...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
