Abstract：
BACKGROUND/AIM:Intraperitoneal chemotherapy with taxanes provides high locoregional drug concentrations. Regarding their synergy with hyperthermia, results have been inconclusive. In this in vitro study, the thermal enhancement of the effect of paclitaxel and docetaxel on ovarian cancer cells under conditions mimicking those during hyperthermic intraperitoneal chemotherapy (HIPEC) is evaluated. MATERIALS AND METHODS:Cisplatin-resistant SKOV-3 and OVCAR-3 ovarian cancer cells were exposed for 2 h to 0.1, 1 and 3 μΜ of paclitaxel and docetaxel at 37°C (normothermia) and 41.5°C (hyperthermia). Cell proliferation and cell-cycle distribution were evaluated after 24 h, 3 days and 7 days. RESULTS:A concentration-dependent cytotoxic effect on cell proliferation was observed. Concurrent hyperthermia caused an increased arrest of cells in the G2/M phase. At 7 days, thermal enhancement of drug effect was shown only for treatment of OVCAR-3 cells with 1 μM paclitaxel. CONCLUSION:The concentration-dependent cytotoxic effect of paclitaxel and docetaxel supports their intraperitoneal use. Due to the lack of or only minimal thermal enhancement, normothermic may be as effective as hyperthermic intraoperative intraperitoneal chemotherapy with taxanes, avoiding, however, potential oncological and treatment-related adverse effects of concurrent hyperthermia.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
de Bree E,Katsougkri D,Polioudaki H,Tsangaridou E,Michelakis D,Zoras O,Theodoropoulos Pdoi
10.21873/anticanres.14700subject
Has Abstractpub_date
2020-12-01 00:00:00pages
6769-6780issue
12eissn
0250-7005issn
1791-7530pii
40/12/6769journal_volume
40pub_type
杂志文章abstract：:The objective of this study was to discover whether the peptides LRMK and LRMK-Ava linked to the N-terminus of peptides HER-2 (774-788) and HER-2 (776-788), respectively, help differentiation of E75-TCR(+)CD8(+) cells. Activation was quantified in terms of proliferation of E75-TCR(+)CD8(+) cells expressing high, mediu...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
abstract：:Membrane permeability is a critical parameter which must be assessed in the course of new potential anticancer drug studies. Flow cytometry allows us to monitor the permeability status of living cells through the measurement of fluorochrome retention; conventionally this is done using fluorescein diacetate or Hoechst3...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：BACKGROUND:Since the anti-HER2 monoclonal antibody trastuzumab made its triumphant advance into breast cancer therapy, new selective agents, including pan-HER inhibitors are entering clinical practice. MATERIALS AND METHODS:This study investigates the expression of the four HER-family members, HER1-4, in 48 primary br...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：AIM:To comprehensively summarize the most important literature regarding the late effects of radiotherapy to the abdomen in childhood and adolescence. MATERIALS AND METHODS:Published trials, studies and series were identified using the PubMed database. The key words late effects, late sequelae, child, radiation, radio...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2010-01-01 00:00:00
abstract：:NMR spectroscopy was used to analyse perchloric acid extracts of normal human brain, murine brain cell cultures, glioblastoma tissue and the glioblastoma cell line U-87. 1H NMR spectra revealed the presence of elevated levels of UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in glioblastoma extracts and the gli...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:Tumor angiogenesis seems to be an important factor in determining therapeutic effectiveness and patient prognosis. An abundance of data on tumor angiogenesis is available and it clearly shows that most human solid tumors are heterogeneously vascularized and oxygenated and contain hypoxic regions. Such regions and area...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2001-11-01 00:00:00
abstract：:In this study, we set out to determine if the differential sensitivities to CPT between a radioresistant (Sk-Mel-3) and a radiosensitive (HT-144) human melanoma cell line, and also between cultures with a different growth phase in each cell line, were related to endogenous differences in cellular transport of CPT or t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：BACKGROUND:Plasmablastic lymphoma (PBL) is a rare entity which is often causally related to infection by the Human Immunodeficiency Virus (HIV). Despite its predilection for oral cavity involvement, multiple cases of extra-oral involvement have been reported in the literature, more often among immunocompetent individua...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：:We generated a recombinant immunotoxin, named scFv(MGR6)-Cla, composed of the Fv region of an anti ErbB2 monoclonal antibody (MGR6) fused to clavin, a type 1 ribosome-inactivating protein (RIP) from Aspergillus clavatus. ErbB2 is a tyrosine kinase receptor which is overexpressed in most adenocarcinomas; clavin is a 17...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Prostatic 25-hydroxyvitamin D-1alpha-hydroxylase (1alpha-OHase) is up-regulated by epidermal growth factor (EGF) and down-regulated by 1alpha,25-dihydroxyvitamin D [1alpha,25(OH)2D] at the promoter level in an autocrine/paracrine fashion, suggesting that local production of 1alpha,25(OH)2D could provide an important c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND/AIM:Gemcitabine is a drug commonly used to treat pancreatic cancer but chemoresistance to it is a common clinical issue. KML001 (sodium meta-arsenite) has demonstrated certain antitumor activity. The objective of the study was to evaluate the influence of KML001 on the anticancer activity of gemcitabine agai...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：:A 67-year-old man presented with leg edema. Laboratory data showed elevated blood glucose and carbohydrate antigen (CA) 19-9 levels, and anemia. Further imaging studies revealed a relatively clear-margined tumor totally occupying the main pancreatic duct (MPD) from the head to the tail of the pancreas (maximum diamete...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-11-01 00:00:00
abstract：BACKGROUND:Radiofrequency ablation (RFA) is one of the standard percutaneous therapies for hepatocellular carcinoma (HCC), but RFA has not been applied to treat bone metastasis from HCC. CASE:A 65-year-old male patient, who underwent hepatectomy for HCC two and a half years previously, complained of pain in his right ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:One of the major obstacles in the treatment of hepatocellular carcinoma (HCC) is the intrinsic/acquired resistance to cisplatin-based chemotherapy. Copper-transporting P-type adenosine triphosphate (ATP7B) has been reported to be associated with cisplatin resistance in vitro. However, the expression of ATP7B has not p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:A human metastatic meningiosarcoma has been established as a cell line as follows: At autopsy performed 12 hours after death, tumour nodules from the left parietal pleura were obtained under sterile conditions, minced and inoculated into culture vessels. The cells obtained were cloned, and clones with abnormal chromos...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:The accumulation of vincristine in leukemic cells and normal mononuclear cells and the effect of verapamil on cellular drug accumulation were studied. Leukemic cells were isolated from 10 patients with chronic lymphocytic leukemia, immunocytoma and prolymphocytic leukemia. Normal mononuclear cells were collected from ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Transferrin was covalently conjugated to adriamycin (Trf-adr) by the formation of a schiff base. This conjugate was found to bind to cell membrane trf receptors in a variety of human tumor cell lines. The Trf-adr conjugate was found to be active against sensitive and resistant cell lines e.g. Lovo, HL-60, H-meso and H...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：BACKGROUND:Melanoma is characterized by high resistance to chemotherapy. The aim of this study was to investigate combined effects of doxorubicin and parthenolide on melanoma cells. MATERIALS AND METHODS:Thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometry were used to evaluate viability. The p53 levels a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：BACKGROUND:Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play a key role in extracellular matrix (ECM) turnover and remodeling. Changes in their expression levels have been observed in various tumor types. However, their clinical significance and prognostic importance in the progr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：:Oxazolinodoxorubicin, a doxorubicin analog with a modified daunosamine moiety was synthesized. The properties of this compound and the parent doxorubicin were compared. The cytotoxicity in vitro studies against several human tumor cell lines (PC-3, MCF-7, SW707, HL-60, RPMI 8226, ACHN) showed higher antiproliferative ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND:Farnesyltransferase inhibitor R115777 (Tipifamib, Zarnestra) is active in breast cancer, but its efficacy in drug combinations has not been extensively investigated. MATERIALS AND METHODS:The activity of R115777 and paclitaxel, alone and in combination, was studied in the human breast cancer cell lines, BT-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：BACKGROUND/AIM:Lobarstin is a metabolite occurring from the Antarctic lichen Stereocaulon alpnum. Human glioblastoma is highly resistant to chemotherapy with temozolomide. Lobarstin was examined for its effect on glioblastoma. MATERIALS AND METHODS:Temozolomide-resistant T98G cells were subjected to toxicity test with...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-12-01 00:00:00
abstract：BACKGROUND:Periacetabular reconstruction after malignant bone tumor resection to preserve limb function is extremely challenging. The optimal reconstruction method has not yet received consensus and the functional outcomes still remain unsatisfactory. CASE REPORT:A thirteen-year-old girl who was suffering from acetabu...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:The role of intensive pre- and postoperative chemotherapy in unresectable nonmetastatic neuroblastoma is still controversial. A preoperative regimen that included deferoxamine, cyclophosphamide, etoposide, carboplatin and thiotepa (D-CECaT) was evaluated in 10 children over one year of age at diagnosis, and this was f...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：UNLABELLED:Adenomatous polyps are precursor lesions for colorectal carcinoma. The risk of cancer development has been associated with age and size, amount of villous component and high-grade dysplasia of adenomas. The subject-related and adenoma-related risk factors for severely dysplastic lesions were further investig...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：:12-O-retinoylphorbol-13-acetate (RPA), an incomplete tumor promoter of the phorbol ester type and protein kinase C (PKC) activator, consists of two characteristic structural elements: the phorbol body and the retinoyl ester chain. Therefore, possible binding of the incomplete tumor promoter RPA to the human transport ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Metallothioneins (MT) are low molecular weight cysteine-rich proteins, present in a wide variety of eukaryotes. Although their physiological function is not entirely understood, recently it was found that in vitro human MTs (hMTs) expression prevents apoptosis. In the present study, the apoptosis preventing effect of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:Mammalian cells transfected with the S. cerevisiae APN1 protein acquire resistance to oxidizing agents, the damage of which is mainly repaired via DNA base excision repair (BER). We have recently hypothesized that this effect might be linked to the possible capacity of APN1 to accelerate mammalian BER by its 3' dieste...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:The aim of this study was to evaluate the usefulness of image-guided robotic stereotactic radiosurgery for the local control of unresectable liver metastases from colorectal and non-colorectal cancer. Twenty-seven consecutive patients (median age 62 years, range 47-80 years) with liver metastases considered unsuitable...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：:We have previously described that thymic lymphomas from anti HY-TCR transgenic mice were resistant to TCR-induced (calcium-mediated) apoptosis but sensitive to induction of apoptosis by etoposide. A defect of apoptosis in these cells is located downstream of the nuclear receptor Nur77 induction and upstream from the e...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
