Abstract：
:Membrane permeability is a critical parameter which must be assessed in the course of new potential anticancer drug studies. Flow cytometry allows us to monitor the permeability status of living cells through the measurement of fluorochrome retention; conventionally this is done using fluorescein diacetate or Hoechst33342. In the present study we show that Texas-Red (TR) can be advantageously used for the same purpose because: (i) it possesses fluorescence spectra compatible with the simultaneous use of various fluorochromes; (ii) staining is rapid; (iii) it is a viability marker. TR uptake is realized through an active, energy dependent transport across the membrane. This new technique was applied to exponentially growing HL60 and their differentiated counterparts. We show that membrane permeability varies throughout the cell cycle progression and during differentiation. We also show that TR uptake is different between sensitive and cytotoxic drug resistant cells. This technique could be used, in anticancer pharmacology, to correlate phase specificity of cytotoxic drugs with membrane permeability, to understand better the mechanism of action of differentiating agents, and to document the apparition of resistant clones within populations of cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Leonce S,Cudennec CAsubject
Has Abstractpub_date
1990-03-01 00:00:00pages
369-74issue
2Aeissn
0250-7005issn
1791-7530journal_volume
10pub_type
杂志文章abstract：BACKGROUND:P-glycoprotein (Pgp), encoded by the ATP-binding cassette B1 (ABCB1) gene, is an efflux transporter located on the luminal side of intestinal epithelial cells, which protects the gut from endogenous and exogenous toxins. The association of two ABCB1 polymorphisms with the occurrence of colon cancer and long-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-11-01 00:00:00
abstract：:The p53 gene product suppresses both tumor cell growth and the cellular transformation process promoted by oncogenes. Although several genes are known to be positively regulated by p53, it is unclear how many gene expression events are involved in p53-mediated growth arrest and apoptosis. Two-dimensional gel electroph...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:As with SV40-transformed BHK cells (1), pretreatment of BHK cells with 1-beta-D-arabinofuranosylcytosine (araC) very markedly enhanced the resistance frequency to N-phosphonoacetyl-L-aspartate (PALA) but only modestly increased the resistance frequency to vincristine (VCR). AraC pretreatment of cells that already had ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:Molecular alterations such as DNA microsatellite instability (MSI/RER), single nucleotide polymorphism (SNP) and loss of heterozygosity (LOH) can occur throughout the genome and be associated with different types of cancer. In the present study, we aimed at detecting molecular alterations within the mismatch...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:Measurement of cell survival as performed by the differential staining cytotoxicity (DiSC) assay allows for a comprehensive prediction of individual chemotherapy response. This assay has not reached routine clinical application because of its several limitations. In order to improve the DiSC assay, we develo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-06-01 00:00:00
abstract：:CA 72-4 is a high molecular weight, pancarcinoma human tumor mucin which may play an important role in the identification (i.e., staging) and clinical management of patients with gastric carcinoma. In the present study of 242 patients with primary or recurrent gastric cancer, a higher percentage of these patients had ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The development of multidrug resistance (MDR) causes difficulties in the chemotherapy of of human cancer. Investigation of the possibility of reversal of MDR has been greatly aided by the use of cell lines with acquired resitance to anticancer agents in vitro or transfected with the mdrl gene. The aim of this study wa...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：AIM:The purpose of the present study was the analysis of the relationship between tumor necrosis factor-α (TNF-α) -308 G/A and cervical intraepithelial neoplasia (CIN). MATERIALS AND METHODS:A prospective case-control study was performed, enrolling 78 cases of cervical intraepithelial neoplasia and 107 controls. Every...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:Metallothioneins (MT) are low molecular weight cysteine-rich proteins, present in a wide variety of eukaryotes. Although their physiological function is not entirely understood, recently it was found that in vitro human MTs (hMTs) expression prevents apoptosis. In the present study, the apoptosis preventing effect of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:Several studies have reported an association between Hashimoto's disease and thyroid carcinoma although the cause/effect relationship is still controversial. CASE REPORT:In this paper we report the case of a 38-year-old female who first presented with a clinical history of Hashimoto's thyroiditis. Ultrasoun...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Inv(3)(q21q26)/t(3;3)(q21;q26) is a feature of a distinctive entity of acute myeloid leukemia (AML) associated with normal or elevated platelet count, atypical megakaryocytes and multilineage dysplasia in the bone marrow, as well as minimal to no response to chemotherapy and poor clinical outcome. The presence of an i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-02-01 00:00:00
abstract：BACKGROUND/AIM:The hormonally-active form of vitamin D, 1,25(OH)2D3, demonstrated activity against oral squamous cell carcinoma (OSCC). Cytochrome P450scc (CYP11A1)-derived vitamin D hydroxyderivatives, such as 20(OH)D3 and 1,20(OH)2D3, have overlapping beneficial effects with 1,25(OH)2D3 without causing hypercalcemia....
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14216
更新日期：2020-05-01 00:00:00
abstract：:This work includes results from studies on oesophageal cytopathology, dietary studies and the correlation of oesophageal cytological abnormalities and micronutrient blood levels in populations at risk for oesophageal carcinoma (OC) in remote rural areas of Southern Africa. The incidence of malignant lesions was 1.6 - ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:The antitumor effect of a polyamine biosynthetic pathway inhibitor methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP) on human osteosarcoma cell lines such as KHOS-240S, MG-63 and G-292 cells, and its effect in combination with anticancer drugs such as methotrexate (MTX), adriamycin (ADM) and 4-hydroperoxyfosfamid...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:We have developed a chemosensitivity assay of human tumors growing on collagen-sponge-gel-supported histoculture. This assay is thus termed the Histoculture Drug Response Assay (HDRA). In the HDRA, the end points of [3H]thymidine incorporation measured by histological autoradiography and tetrazolium dye reduction were...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：AIM:To identify patients with breast cancer at risk for cardiotoxicity, we evaluated homoarginine (HA) behavior during adjuvant treatment. PATIENTS AND METHODS:Eighty-one patients received radiotherapy (RT) with or without endocrine treatment, and 19 received chemotherapy, RT and endocrine therapy. Serum HA, asymmetri...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12142
更新日期：2017-12-01 00:00:00
abstract：UNLABELLED:AIM/ BACKGROUND: To determine the feasibility and safety of intratumoral α-gal glycolipids injection for conversion of human tumors into autologous Tumor Associated Antigens (TAA) vaccine. α-Gal glycolipids bind anti-Gal--the most abundant antibody in humans. Pre-clinical studies indicated that injected α-ga...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：:The antitumoral and immunostimulating properties of rViscumin (recombinant mistletoe lectin) were investigated in two mouse tumor models. After intravenous inoculation with RAW-117-P or L-1 sarcoma cells in Balb/c mice, rViscumin was given s.c. at non-toxic doses ranging from 0.3 to 150 ng rViscumin/kg. One set of exp...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:The expression of the proto-oncogene c-myc has been strongly implicated in the regulation of apoptosis. Since we have previously shown apoptosis to occur in HL-60 leukemic cells exposed to the prodrug Mafosfamide (ASTA Z 7557), the present study was undertaken to examine the levels of c-myc mRNA transcripts in such ce...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：BACKGROUND:Primary serous-papillary peritoneal carcinoma is able to spread to the retroperitoneal lymph nodes, but there is no evidence of the pattern of lymphatic metastasis. Because of the fact that this tumor entity is indistinguishable histologically from primary serous-papillary ovarian carcinoma, its taxonomic po...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2011-04-01 00:00:00
abstract：BACKGROUND:Treatment for advanced pancreatic cancer is still very unsatisfactory. Treosulfan is an alkylating agent used for conventional, as well as high-dose chemotherapy regimens, whereby plasma concentrations over 500 μg/ml can be achieved. We investigated the effects of treosulfan on pancreatic cancer cell lines. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：AIM:The aim of the study was to identify novel biomarkers that are vital for improving management of patients with gastric cancer (GC). MATERIALS AND METHODS:An RNA-sequencing analysis was conducted using gastric tissue from patients with metastatic GC. In vitro cell functions were evaluated by siRNA-mediated knockdow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13820
更新日期：2019-11-01 00:00:00
abstract：BACKGROUND:NSC697923, a ubiquitin-conjugating enzyme E2 (UBE2) inhibitor, was suggested as an agent to degrade hypoxia-inducible factor 1 alpha subunit (HIF1α), a key factor in radiation resistance. We attempted to clarify whether NSC697923 could overcome radiation resistance. MATERIALS AND METHODS:Radiation resistanc...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11582
更新日期：2017-05-01 00:00:00
abstract：OBJECTIVE:To investigate the role of body composition and oxidative stress measured by total thiol groups (TTG) levels in prostate specimens of patients affected by benign prostatic hyperplasia (BPH) or prostate cancer (PCa). PATIENTS AND METHODS:From January 2011 to January 2013, a cohort of 150 consecutive male pati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：:Xenograft hyperacute rejection in humans occurs as a secondary response to a cellular glycosylation incompatibility with most non-human mammalian species. A key component of hyperacute rejection, alpha(1,3)galactosyl (agal) epitopes present on the surface of most non-human mammal cells, is bound by host anti-agal IgG ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:The small molecular weight protein (p7) was overexpressed in the human ovarian carcinoma cell lines, SKVLB600 (selected for vinblastine resistance from SKOV3 cells). OVCAR 4/ADR100 (selected for doxorubicin resistance from NIH:OVCAR4) and (OVCAR4/VBL200 (selected for vinblastine resistance from NIH:OVCAR4). Trace amou...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND:Although paclitaxel was given triweekly in phase II trials prior to its approval for gastric cancer in Japan, it is currently more often delivered by a weekly schedule in the second-line setting. PATIENTS AND METHODS:A phase II trial with response rate as the primary end-point was conducted. Patients with m...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：BACKGROUND:The aim of this study was to assess the metallothionein (MT), maintenance protein 2 (Mcm-2) and Ki-67 expressions in adrenocortical adenomas and carcinomas in comparison to normal tissue and evaluate the correlations between these markers of proliferation and between these markers and tumor diameter. MATERI...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：BACKGROUND:Most tumor tissues, especially those of non-hematopoietic origin, do not express CD80 co-stimulatory molecules, possibly as a mechanism to evade immune surveillance. The objective of this study was to determine whether abundant endogenous CD80 expression on oral squamous cell carcinoma (SCC) early during tum...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：:Screening for hereditary tumours provides a basis for secondary prevention. In 1985, the Foundation for the Detection of Hereditary Tumours was established in The Netherlands to coordinate screening in families with hereditary tumours. In the initial period of four years a large number of families were collected. Regi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
