Abstract:
AIM:Eudragit® E 100 (EE100) was used to improve the transfection efficiency of polyethylenimine (PEI). MATERIALS AND METHODS:Mobility of PEI-DNA complexes with and without EE100 were visualized by agarose gel electrophoresis and their transfection efficiencies were investigated in KB human oral carcinoma cells by flow cytometry. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to determine the viability of transfected cells. RESULTS:Gel electrophoresis illustrated formation of complete complexes at N/P ratios above 5. PEI had the highest transfection efficiency at an N/P ratio of 15, whereas in combination with EE100, the transfection efficiency was highest at an N/P ratio of 7.5. High concentrations of EE100 in combination with PEI were found to reduce cell viability. CONCLUSION:The results show a synergistic action of EE100 in transfection of DNA at low N/P ratios compared to PEI alone.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Kanthamneni N,Yung B,Lee RJsubject
Has Abstractpub_date
2016-01-01 00:00:00pages
81-5issue
1eissn
0250-7005issn
1791-7530pii
36/1/81journal_volume
36pub_type
杂志文章abstract::The authors report their experience in the radioimmunodetection of recurrent colorectal cancer in comparison with traditional examinations. 485 patients were studied after radical surgery for colorectal cancer: 168 (Group 1) were studied with a radioimmunoassisted follow-up plan including: Immunoscintigraphy (IS), ser...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::Adenovirus(ADV) mediated thymidine kinase(TK) gene therapy followed by ganciclovir(GCV) administration is widely used in different types of cancer. ACV shares the same mechanism of selective cell killing in ADV/TK positive cells as GCV and can be used at 4.5 times higher doses in patients without significant side effe...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract:BACKGROUND/AIM:Breast cancer is the most prevalent and devastating malignant disease among women worldwide. Green tea has been extensively studied for its anti-cancer effects, however, existing literature on the correlation of other types of tea with breast cancer is very limited. MATERIALS AND METHODS:We used six dif...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12976
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. MATERIALS AND METHODS:Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95)...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Glycerophosphoinositols are metabolites formed by the sequential action of a phospholipase A2 specific for membrane phosphoinositides, and a lysolipase. Increased levels of these compounds characterize cell lines of different origin transformed by ras and other oncogenes. Thus, glycerophosphoinositols have been propos...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND/AIM:The Cancer Stem Cell (CSC) model proposes that cancer is driven by a cellular component which possesses stem cell (SC) properties, cancer stem cells (CSCs), a distinct cell-type which is tumorigenic and capable of invasion and metastasis. Enzymatic activity of aldehyde dehydrogenase-1 (ALDH1), a de-toxif...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Big Endothelin-1 levels increase significantly in patients with various tumors, and raised plasma concentrations are associated with worse outcome. The aim of this study was to investigate plasma Big Endothelin-1 levels in patients with gastric carcinoma before and after radical gastrectomy and to explore it...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Angiogenesis is essential for tumour growth. Mostly hypervasculariced renal cell carcinoma (RCC) usually express angiogenic factors, e.g. basic fibroblast factor (bFGF), vascular endothelial growth factor, and angiogenin (ag). This study was designed to evaluate the improvement of serological angiogenesis-factors, the...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract:BACKGROUND:Oleanolic acid is a triterpenoid that has shown in vitro cytotoxic activity against human tumour cells and is known to be present in many higher plants. MATERIALS AND METHODS:Oleanolic acid is known to have some biological potential including anticancer property. Oleanolic acid was isolated from the ethyl a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Since superparamagnetic iron oxide nanoparticles (SPION) possess unique features, they provide a huge platform for medical applications, especially for cancer diagnosis and therapy (e.g. imaging, and drug targeting). However, heterogeneous effects on mammalian cells with regard to reproductive tissue are des...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-06-01 00:00:00
abstract::Dendritic cells (DCs) are professional antigen presenting cells. Two x 10(7) peripheral blood mononuclear cells (PBMCs) from each of 6 melanoma patients and 4 normal donors were incubated in 6-well plates. The adherent cells were cultured in GM-CSF (800 U/ml) and IL-4 (1000 U/ml) for 10 days. Anti-CD80, anti-CD86, ant...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::The effect of various physiological fluids on the radical intensity of sodium ascorbate and sodium 5,6-benzylidene-L-ascorbate (SBA) was investigated using ESR spectroscopy. Blood from various animals did not significantly affect the radical intensity of both ascorbates, whereas the corresponding plasma fractions sign...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Cytogenetic and DNA flow cytometric analysis was carried out on four SV40T-transfected human buccal epithelial cells lines. One of these was immortalized and showed a nontumorigenic phenotype when tested in athymic nude mice. DNA flow cytometric ploidy values correlated well with cytogenetic ploidy values as calculate...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND/AIM:Despite numerous studies, the etiology of chronic lymphocytic leukemia (CLL) remains unknown. A hypothesis of autoantigen stimulation in leukemic clone selection might explain 'stereotypy' of B-cell receptors. In healthy cells, cofilin-1 (CFL1) has multiple functions. Its role was described in several ma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14653
更新日期:2020-11-01 00:00:00
abstract::Five functionalized benz(c)acridines - 5,6-dimethylbenz(c)acridine; 5,6,7-trimethylbenz(c)acridine; 7-chloro-5,6-dimethylbenz(c)acridine; 7-amino-5,6-dimethylbenz(c)acridine; 7-oxo-5,6-dimethylbenz(c)-acridine and 5,6-dimethylbenz(a)phenazine - were tested for mutagenic activity in the Ames Salmonella typhimurium assa...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Monoclonal antibodies TP-1 and TP-3, which detect an antigen expressed on the cells of human osteosarcoma, were tested by immunohistochemical staining for binding to normal and neoplastic canine tissues. In dogs, as in man, TP antibodies stained the tumor cells of all (13/13) osteosarcomas tested. Limited staining was...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract::Consumption of dietary fat has been linked to the high incidence of certain cancers. However, recent research has stimulated interest in conjugated linoleic acid (CLA), a newly recognized anticarcinogenic fatty acid. Human MCF-7 breast cancer cells were incubated for 12 d in culture medium supplemented with various co...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::The magnetic resonance (MR) findings in malignant melanoma of soft tissues, also called clear cell sarcoma of tendons and aponeuroses, have been described as a focal abnormality with a specific MR pattern of increased signal intensity (relative to normal muscle) on T1 weighted sequences and variably decreased signal i...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2000-09-01 00:00:00
abstract:BACKGROUND:This study aimed to examine the effect of apigenin on proliferation and apoptosis in HER2-overexpressing MDA-MB-453 breast cancer cells. MATERIALS AND METHODS:The antiproliferative effects of apigenin were examined by proliferation and MTT assays. The effect of apigenin on apoptotic molecules was determined...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract::The present study was undertaken to evaluate the significance of grooved nuclei as an additional diagnostic criterion for primary breast carcinoma as well as their association with tumor grade in cytologic material obtained by fine needle aspiration (FNA). Cytologic slides of 105 cases of breast carcinoma (89 ductal, ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:Green tea polyphenols (GTPs) have been proposed as promising candidates for chemoprevention. However, GTPs levels are maintained relatively low in the blood and are chemically-unstable. Lipid-soluble tea polyphenols (LTPs) are products of modified GTPs with ester linkage with fatty acids. LTPs are lipophilic...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND/AIM:No standard treatment has been established for poorly-differentiated neuroendocrine carcinomas (PDNEC). The aim of this study is to evaluate the response to the combination of three drugs. MATERIAL AND METHODS:We reviewed 21 PDNEC patients treated from 2008 to 2013 in two Institutions. Five patients wer...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
abstract::260 CB57BL/J6 mice were used in an experimental protocol designed to investigate the effects of 4 different varieties of splenectomy on the growth rate of subcutaneously implanted GB-16 melanoma. In addition, the mean and absolute survival of the mice, the histopathology of the tumour and the effects of the same proce...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND/AIM:Non-pegylated liposomal doxorubicin (NPLD) has demonstrated antitumour activity equivalent to conventional doxorubicin and a significantly lower risk of cardiotoxicity. This phase II trial was performed to evaluate the activity and the safety of NPLD and ifosfamide combination in patients with metastatic...
journal_title:Anticancer research
pub_type: 杂志文章,多中心研究
doi:
更新日期:2015-01-01 00:00:00
abstract::Germ line point mutations in the RET proto-oncogene have been implicated in four inherited disorders: multiple endocrine neoplasia 2A (MEN 2A) and 2B (MEN 2B); familial medullary thyroid carcinoma (FMTC); and Hirschprung's disease, a congenital lack of enteric plexus neurons. Oncogenically activated RET has also been ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract:AIM:To assess outcomes in patients treated with first-line bevacizumab-containing therapy for human epidermal growth factor receptor (HER)2-negative metastatic breast cancer (mBC) at a single centre with a homogenous standard-of-care. PATIENTS AND METHODS:Information on patient and disease characteristics, efficacy, a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11462
更新日期:2017-03-01 00:00:00
abstract::The effects of the 18 carbon chain length series of fatty acids on the viability of a 3T6 derived mouse fibroblast cell line were tested. An overall concentration dependent cytotoxic effect was demonstrated with the saturated fatty acid, and cis- and trans- mono- and dienoic fatty acids; however, within a narrow range...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Chemotherapy for patients with multiple myeloma was individually selected on the basis of antiproliferative drug sensitivity determined in vitro by a clonogenic assay. Myeloma stem cells were preincubated with various cytostatic drugs and allowed to form colonies in a plasma clot culture system. In 21 out of 24 prospe...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract:BACKGROUND:Although oncological results for resection of colon cancer with inferior mesenteric artery (IMA) ligation at its origin (high tie) or immediately below the left colic artery (low tie) are similar, injury of the superior hypogastric plexus may be caused when the IMA is tied at its origin, thus impairing defec...
journal_title:Anticancer research
pub_type: 杂志文章,随机对照试验
doi:10.21873/anticanres.14276
更新日期:2020-05-01 00:00:00
abstract::2-4 Dinitrochlorobenzene (D.N.C.B.) is a synthetic primary allergenic molecule which has proved to have at least two useful clinical applications as regards neoplastic conditions. As a diagnostic measure, it serves in the detection of global alterations of cellular immunity. As a therapeutical measure, its epicutaneou...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1989-11-01 00:00:00