Effect of Eudragit on In Vitro Transfection Efficiency of PEI-DNA Complexes.

Abstract:

AIM:Eudragit® E 100 (EE100) was used to improve the transfection efficiency of polyethylenimine (PEI). MATERIALS AND METHODS:Mobility of PEI-DNA complexes with and without EE100 were visualized by agarose gel electrophoresis and their transfection efficiencies were investigated in KB human oral carcinoma cells by flow cytometry. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to determine the viability of transfected cells. RESULTS:Gel electrophoresis illustrated formation of complete complexes at N/P ratios above 5. PEI had the highest transfection efficiency at an N/P ratio of 15, whereas in combination with EE100, the transfection efficiency was highest at an N/P ratio of 7.5. High concentrations of EE100 in combination with PEI were found to reduce cell viability. CONCLUSION:The results show a synergistic action of EE100 in transfection of DNA at low N/P ratios compared to PEI alone.

journal_name

Anticancer Res

journal_title

Anticancer research

authors

Kanthamneni N,Yung B,Lee RJ

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

81-5

issue

1

eissn

0250-7005

issn

1791-7530

pii

36/1/81

journal_volume

36

pub_type

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