Abstract：
PURPOSE:Investigation of the oxygenation status of organotypic multicellular spheroids (OMS) and their response to irradiation. MATERIALS AND METHODS:Tumour specimens of glioblastoma multiforme patients (n = 16) were initiated as OMS. Following 20 Gy gamma-irradiation, the cell migratory capacity was evaluated. Spheroid oxygenation was determined by micro-electrode pO2 measurements and pimonidazole immunostaining. Spheroids prepared from established human glioma cell lines were used as a reference. RESULTS:Irradiation inhibited spheroid outgrowth by 12 to 88% relative to the non-irradiated controls. A large interpatient variation was noticed. Oxygen measurements revealed a gradual decrease in pO2 level from the periphery to the core of the spheroids, but the pO2 values remained within an oxygenated range. However, in the cell line spheroids an intermediate layer of hypoxia surrounding the central core was observed. CONCLUSION:Cell line spheroids with a hypoxic cell fraction and well-oxygenated OMS both show high resistance to irradiation, indicating that hypoxia may not be the biological factor determining the radioresistance of glioma spheroids in vitro.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Sminia P,Acker H,Eikesdal HP,Kaaijk P,Enger Pø,Slotman B,Bjerkvig Rsubject
Has Abstractpub_date
2003-03-01 00:00:00pages
1461-6issue
2Beissn
0250-7005issn
1791-7530journal_volume
23pub_type
杂志文章abstract：:Cancer patients suffer from cancer-induced bone pain, hypercalcemia, and reduced quality of life caused by pathological fractures. Many of these complications related to cancer can be treated, or at least controlled, using new anticancer agents. Recently, two agents used initially to treat osteoporosis demonstrated di...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2013-08-01 00:00:00
abstract：:Multidrug resistance (mdr) constitutes the major obstacle to successful cancer treatment. The ability of fifteen newly-synthesised N-acylphenothiazine derivatives to inhibit the transport activity of P-glycoprotein was studied by flow cytometry in a resistant mouse lymphoma cell line. A standard functional assay with ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:A rat rhabdomyosarcoma clonal line RMS/8 was cotransfected with genomic DNA of procaryotic or eucaryotic origin and neomycin resistance plasmid pKOneo. The lung colonizing and metastatic capacity of transfected cells was evaluated. When col- meta- RMS/8 cells were transfected, an increase in lung colonizing but not in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:Alphafetoprotein (AFP) is an oncofetal glycoprotein that frequently reappears in sera of patients affected by hepatocellular carcinoma (HCC) and yolk-sac tumours (YST). In these cases AFP determination represents a useful marker for monitoring the therapeutic response and the clinical evolution of the neoplastic disea...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1995-07-01 00:00:00
abstract：BACKGROUND:Acquired resistance to platinum-based chemotherapy (Pt-chemo) is a major problem for improving the prognosis for patients with advanced epithelial ovarian cancer (EOC). However, the molecular mechanism of acquired resistance to Pt-chemo is not well understood. MATERIALS AND METHODS:hMLH1 promoter methylatio...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:The mammalian target of rapamycin (mTOR) controls cell growth through protein synthesis regulation. It can be activated by protein kinase B (AKT) or through ribosomal S6 kinase (S6K1). In malignant glioma, mTOR inhibitors have antiproliferative and proapoptotic effects and mTOR has been suggested as a target...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：:The role of growth factors in tumour growth and progression has increasingly been studied over the last few years. This review summarizes the available data and discusses their limitations as well as their potential influence on future therapeutic strategies. A large body of data suggests an important role of EGF, TGF...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2003-03-01 00:00:00
abstract：:This review focuses on the functional role and structural features of the genes involved in common hereditary cancers. Most of these tumors are sporadic and the genetic alterations responsible for their genesis take place over several cell generations; nevertheless, 5 to 10% of the human tumors are hereditary, with a ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND/AIM:To compare the GeneXpert® O6-methylguanine DNA methyltransferase (MGMT) methylation prototype (GX MGMT) assay with pyrosequencing in glioblastomas. MATERIALS AND METHODS:The MGMT methylation status was retrospectively assessed in formalin-fixed paraffin embedded (FFPE) tumor blocks from 262 glioblastoma...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14643
更新日期：2020-11-01 00:00:00
abstract：BACKGROUND/AIM:To investigate whether surgical margin (SM) status would affect the biochemical recurrence (BCR) after robot-associated RP (RARP). PATIENTS AND METHODS:We evaluated BCR after RARP and the association between pre- and postoperative predictive factors and BCR. RESULTS:Positive SM (PSM) was observed in 97...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14802
更新日期：2021-01-01 00:00:00
abstract：:Cisplatin toxicity could be decreased by adjusting its dosage to each patient. For this purpose, a limited sampling method was established and validated based on a Bayesian approach taken using the values of assays during a 5-day continuous infusion of cisplatin. Using this method, a dosing model to achieve a target p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND:Patients with advanced pancreatic carcinoma have a risk of relapse after primary therapy, and the prognosis for these patients remains bleak. The effect of immuno-cell therapy in advanced pancreatic carcinoma, with or without other standard therapies, was examined. PATIENTS AND METHODS:Forty-six patients wi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:We studied a consecutive series of 54 cases of lower lip squamous cell carcinoma (LLSCC) in order to identify any variables which might predict the development of lymph node metastases. The cases were divided into 38 tumors without metastases (group I) and 16 tumors with lymph node metastases (group II). The following...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：BACKGROUND:Basic conventional prognostic factors for breast cancer include the age of the patient, tumor grade, regional lymph nodes status, and estrogen (ER) and progesterone (PR) receptor status. Positivity of the HER2 receptor (c-erbB-2) seems to be a new prognostic and predictive factor. Prognostic factors seem to ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：:Due to its diagnostic application, consideration was given to the therapeutic potential of 131I-MIBG in neural crest tumours, mainly in malignant pheochromocytomas, paragangliomas, neuroblastomas (NB), carcinoids and medullary thyroid carcinomas (MTC). The therapeutic procedure consists of a) thyroid blockade; b) admi...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1997-05-01 00:00:00
abstract：:Intraosseous lipomas are rare benign primary bone tumors, with an incidence of one per thousand bone tumors. We studied eleven cases of intraosseous lipoma of the calcaneus. All the patients received radiographic examinations and MRI with T1-and T2-weighted images with Gd-DTPA enhancement. Seven patients received tumo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND:Circulating nucleosomes, complexes of DNA and histones, are recognized as a potential new diagnostic tool for therapy monitoring of cancer disease and as prognostic marker in other acute diseases. Because many studies are carried out retrospectively on serum samples collected earlier, we tested the stability...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-05-01 00:00:00
abstract：BACKGROUND:The genetic basis of sinonasal inverted papilloma (SNIP)-derived squamous cell carcinoma (SCC) has not yet been well characterized. AIM:To characterize the genetic abnormalities of SNIP and SNIP-derived SCC and to uncover their differences. MATERIALS AND METHODS:Mutations of 409 genes were analyzed using a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14752
更新日期：2021-01-01 00:00:00
abstract：BACKGROUND:Cancer of the small intestine is a rare disease, and its clinical features have not been clearly elucidated. Techniques such as double balloon endoscopy and capsule endoscopy allow the preoperative diagnosis of cancer of the small intestine, but this cancer is often detected at an advanced state and in many ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-08-01 00:00:00
abstract：BACKGROUND/AIM:Glioblastoma multiforme (GBM) is a malignant primary brain tumor with high rates of recurrence. This study aimed to investigate the effect of repurposed drug combinations on GBM. MATERIALS AND METHODS:Viability of U87 MG and 11ST patient-derived GMB cell lines, after valproic acid, tranylcypromine or ri...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13099
更新日期：2019-01-01 00:00:00
abstract：AIM:Carbonic anhydrase 9 (CA9) has been found to be one of most powerful biomarkers for clear-cell renal cell carcinoma (RCC). The serum CA9 is detectable. The aim of this study was to evaluate the potential prognostic role of serum CA9 in patients with metastatic clear-cell RCC patients under targeted therapy. PATIEN...
journal_title：Anticancer research
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2012-12-01 00:00:00
abstract：:2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) is a food mutagen which has been found to be carcinogenic in the Fischer-344 rat. We have assessed the effects of a diet containing menhaden oil on IQ-DNA adduct formation in several organs of the male Fischer-344 rat. Young adult, male Fischer-344 rats were maintained on e...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：BACKGROUND:The albumin-bilirubin (ALBI) grade assesses the severity of liver dysfunction in patients with hepatocellular carcinoma. Herein we investigated the prognostic significance of the combination of the ALBI grade with serum carbohydrate antigen 19-9 (CA 19-9) concentration, the most frequently used tumor marker ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13260
更新日期：2019-03-01 00:00:00
abstract：:The presence of steroid receptors in breast cancer is usually considered as a good indicator for responsiveness to antiestrogen treatment. However, not all estrogen-receptor (ER) positive tumors respond to tamoxifen, probably owing to the natural heterogeneity of breast cancer, which after tamoxifen treatment can resu...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：BACKGROUND/AIM:In the last years, the use of Image Guided Stereotactic Radiotherapy (IG-SBRT) in patients with metastatic prostate cancer has increased. In this study, we aimed to assess the role of IG-SBRT in terms of local control and safety in patients with metastatic prostate cancer. MATERIALS AND METHODS:Primary ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12572
更新日期：2018-05-01 00:00:00
abstract：:Initial events in the metastatic spread of tumours involve loss of cell-cell adhesion within the primary tumour mass. The integrity and morphology of epithelial tumour cell colonies is maintained primarily by cell-cell adhesions mediated by E-cadherin and its associated intracellular catenin molecules. Hepatocyte grow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Carcinoma cell metastasis is mediated by increased mobility and invasiveness via epithelial-mesenchymal transition (EMT). Snail, a zinc-finger transcription factor, triggers EMT by directly repressing E-cadherin expression. In this study, the correlation between E-cadherin and Snail expression was investigated. In add...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：BACKGROUND/AIM:Seizures represent a major problem for patients with brain metastases. This study evaluated the role of seizures in patients receiving single-fraction radiosurgery (SRS) or multi-fraction stereotactic radiotherapy (FSRT). PATIENTS AND METHODS:This retrospective study included 195 patients receiving SRS ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14337
更新日期：2020-06-01 00:00:00
abstract：AIM:After radical prostatectomy, PSA levels that reach near zero values in less than 14 days are associated with a favourable prognosis. The aim of this analysis was to investigate whether PSA also declines to near zero values after combined tele-brachytherapy. MATERIALS AND METHODS:Forty-one patient, treated with com...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND:Doxorubicin remains one of the few drugs with consistent single agent activity in advanced Soft Tissue Sarcomas (STS), with a demonstrated dose-response relationship. Liposomal-encapsulated Doxorubicin (LED) has been shown to be at least as active as free doxorubicin in experimental models, and phase I and I...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-01-01 00:00:00
