Abstract:
BACKGROUND:Circulating nucleosomes, complexes of DNA and histones, are recognized as a potential new diagnostic tool for therapy monitoring of cancer disease and as prognostic marker in other acute diseases. Because many studies are carried out retrospectively on serum samples collected earlier, we tested the stability of nucleosomes during long-term storage at -70 degrees C. PATIENTS AND METHODS:Never-thawed aliquots of 154 stabilized serum samples from cancer patients which had been stored between 11/1999 and 07/2001 at -70 degrees C were analyzed by ELISA six to nine months after collection and for a second time in 11/2006, with a median interval of 64.8 months (+/-5.5 months) between the measurements. RESULTS:Nucleosome levels of the second measurement (median 220.0 ng/ml) were significantly lower than those of the first measurement (354.0 ng/ml; p<0.0001) exhibiting a median decrease of 32.0% (mean 29.7%+/-39.7%). Single concentrations of both measurements correlated well (R=0.92; p<0.0001). Individual courses of eight cancer patients showed a parallel downshifting of the nucleosome signal. The concordance rate of parallel increases and decreases was 89%. CONCLUSION:Retrospective studies on nucleosomes in serum can be performed if samples derive from homogeneous time intervals and preanalytical protocols are respected.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Holdenrieder S,Von Pawel J,Nagel D,Stieber Psubject
Has Abstractpub_date
2010-05-01 00:00:00pages
1613-5issue
5eissn
0250-7005issn
1791-7530pii
30/5/1613journal_volume
30pub_type
杂志文章abstract::The recent meeting of the AGMIF (Working Group for Mistletoe Therapy and Immunological Research) held in Herdecke, Germany, on October, 3rd, 1997, covered recent developments in the field of immunological and biological properties of Viscum album L., the European mistletoe, which is used for adjuvant cancer treatment....
journal_title:Anticancer research
pub_type:
doi:
更新日期:1998-09-01 00:00:00
abstract::The effect of alpha-Interferon (alpha-IFN), gamma-interferon (gamma-IFN), tumor necrosis factor alpha (TNF-alpha) and interleukin-2 (Il-2) on growth of two renal cancer cell lines (DNT-11, HTB-44) was studied. Cell proliferation was measured by a tetrazolium based microculture assay (MTT) after incubation of tumor cel...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:This study aimed to elucidate the prognostic value of vimentin expression in patients with intrahepatic cholangiocarcinoma (ICC). PATIENTS AND METHODS:A retrospective analysis of 21 patients who underwent resection for ICC was conducted. Vimentin expression was positive when single tumour cells or cell clus...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND/AIM:The hormonally-active form of vitamin D, 1,25(OH)2D3, demonstrated activity against oral squamous cell carcinoma (OSCC). Cytochrome P450scc (CYP11A1)-derived vitamin D hydroxyderivatives, such as 20(OH)D3 and 1,20(OH)2D3, have overlapping beneficial effects with 1,25(OH)2D3 without causing hypercalcemia....
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14216
更新日期:2020-05-01 00:00:00
abstract::In a randomised, double-blind study, the efficacy of sodium selenite application in combination with physical therapy to relieve congestion was investigated in a cohort of 60 cancer patients with secondary lymphedema, with special reference to the development of the incidence of erysipelas. All of the patients investi...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1998-05-01 00:00:00
abstract::Because of its poor prognosis, new modalities to treat pancreatic cancer are highly welcome. Gammalinolenate (GLA) has been shown to possess antitumor activity on various human cancer cell lines in vitro and some evidence has been found of its modulative activity on tubulin active agents, such as vinca alkaloids. GLA ...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract:AIM:To evaluate the frequency of loss of mediator of DNA damage checkpoint protein 1 (MDC1) protein expression in endometrial cancer (EC) and to determine whether loss of MDC1 is associated with certain clinicopathological parameters. MATERIALS AND METHODS:MDC1 expression was examined in 426 samples of EC. The nuclear...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13870
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:The mitochondrial protein translocase of inner mitochondrial membrane 17 homolog A (TIMM17A) has been identified as a biomarker of breast cancer. The present study aimed to investigate the biological role of TIMM17A in human breast cancer cells. MATERIALS AND METHODS:Anti-TIMM17A transgenes were stably tran...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND/AIM:The European MRI and Rectal Cancer Surgery (EuMaRCS) score was proposed to identify preoperatively difficult laparoscopic total mesorectal excision (L-TME) for locally advanced rectal cancer (LARC). This study aimed to test EuMaRCS's validity. PATIENTS AND METHODS:Data were retrieved from a European mul...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14166
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:Farnesyltransferase inhibitor R115777 (Tipifamib, Zarnestra) is active in breast cancer, but its efficacy in drug combinations has not been extensively investigated. MATERIALS AND METHODS:The activity of R115777 and paclitaxel, alone and in combination, was studied in the human breast cancer cell lines, BT-...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract::The incidence of breast cancer is increasing rapidly in Taiwan but is still low compared with western countries. The impact of body size factors in western countries inspired us to have a case-control study. A matched case-control study was done on 122 pairs of incident cases of female breast cancer and community cont...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::The effect of PSK, a protein-bound polysaccharide and an immunomodulator, on lymphocytes was examined in vitro for 36 patients with gastric cancer and 26 with colorectal cancer. Cultured lymphocytes with PSK at 100 micrograms/ml increased the level of DNA synthesis, as determined by the 3H-thymidine uptake, from 0.9 t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Four sorts of tumor proliferation-related markers were studied in 50 papillary carcinomas of the thyroid, and the results were correlated with the histologic subtypes of papillary thyroid carcinoma, which have been shown to be associated with the prognosis of the disease. The mitotic count analyzed by light microscopy...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND/AIM:The role of methylation reactions in cancer was examined using the methylating agents, sulfobetaine [dimethylsulfonioproponate (DMSP)], and glycine betaine (GB), in murine crucial Ehrlich ascites carcinoma (EAC) for up to 10 days. RESULTS:DMSP administration in EAC-bearing mice mitigated EAC, while GB a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:Since specific epidermal growth factor receptor (EGFR) somatic mutations have been demonstrated to influence the response to anti-EGFR therapy in non-small cell lung cancer, EGFR gene mutational analysis of different types of neoplasm is under investigation. PATIENTS AND METHODS:EGFR protein expression and ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract:AIM:To identify predictors of poor prognosis of patients with colon cancer (CC) who underwent surgery with curative intent, we investigated the association between the albumin to globulin ratio (AGR) with clinicopathological findings such as overall (OS) and disease-free (DFS) survival. PATIENTS AND METHODS:We conduct...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11452
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Epstein-Barr virus-associated gastric carcinoma (EBV-GC) has been characterized as a special gastric cancer subset. Lifestyle and other major factors that may contribute to EBV-GC and non-EBV-GC were investigated here. MATERIALS AND METHODS:A total of 247 patients with gastric cancer were interviewed, clini...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
abstract::The production of DNA interstrand crosslinks (ISC) by BCNU and other bifunctional alkylators and the effects of these drugs on the repair of radiation-induced DNA-single strand breaks (SSB) were studied in two human glioblastoma used to assess both DNA-ISCs and DNA-SSBs. BCNU-treated UWR2 and UWR3 cells showed a signi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract:BACKGROUND/AIM:Lipitor is a cholesterol-lowering drug and Celebrex is a Cyclooxygenase-2 inhibitor. We investigated the effects of Lipitor and Celebrex on human prostate cancer VCaP cells cultured in vitro and grown as orthotopic xenograft tumors in SCID mice. MATERIALS AND METHODS:Apoptosis was measured by morphologi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract::This trial is based on the strategy of reversing O6-methylguanine-DNA methyltransferase (MGMT)-mediated nimustine resistance by depleting MGMT activity with streptozotocin (STZ) pretreatment. Eight patients with recurrent malignant gliomas refractory to previous nimustine chemotherapy were entered in this study. Patie...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::The oxazaphosphorines cyclophosphamide (CP) and ifosfamide (IF) are alkylating agents that require bioactivation via cytochrome (CYP) P450 isoenzymes including CYP2C9 enzymes. The present study investigated CYP2C9 in regard to its allelic variants in 23 tumor samples (10 breast tumors, 1 breast tumor cell line, 5 brai...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:The concentration of Des-gamma-carboxy-prothrombin (DCP) or PIVKA-II has been described to be increased in patients with hepatocellular cancer, along with its elevation in vitamin K deficient states by warfarin or dicoumarol treatment. The aim of the study was to investigate its clinical value in HCC in comp...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract:AIM:We investigated the immunohistochemical expression of estrogen receptors alpha (ERalpha) and beta (ERbeta) in pituitary adenoma subtypes combined with clinicopathological factors. MATERIALS AND METHODS:Pituitary adenomas (n=75) were immunostained for ERalpha and ERbeta using the streptavidin-biotin-peroxidase comp...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND/AIM:Although the advent of Helicobacter pylori eradication and global societal changes are widely assumed to impact on gastric cancer (GC)-related mortality, there is remarkable little quantitative and qualitative insight into the nature of its effects. Here, we exploited a nationwide reporting system to inv...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11871
更新日期:2017-08-01 00:00:00
abstract::The response of the blood-forming and supportive stromal compartments of the marrow to a localized, fractionated course of radiotherapy [FxRT; 2.0 Gy (q24 h x 5)-->74 Gy] was examined in the C57Bl/6 mouse using the hematopoietic progenitor and the cobblestone-forming cell (CAFC) assays as end-points for the blood-form...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND/AIM:In pancreatic cancer, where the microenvironment is extremely hypoxic, analyzing signal transduction under hypoxia is thought to be significantly important. By investigating microarray analysis of pancreatic cancer cells cultured under both normoxia and hypoxia, we found that the expression of leukocyte ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12122
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:The risk of chronic obstructive pulmonary disease (COPD) is related to Gc protein allele type, such as Gc*1F, Gc*IS, Gc*2. It has been reported that Gc*1F increased COPD risk, while Gc*2 suppressed the risk. Thus, the allele type of Gc protein is an important factor in COPD. These Gc proteins differ in sugar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND:The purpose of the study was to test the feasibility of laser scanning cytometry (LSC) to simultaneously measure estrogen receptor (ER) and nucleolin (NU) expression in the nuclei of the same individual breast cancer cells. MATERIALS AND METHODS:Cancer cells from 64 breast tumors were labeled with anti-NU a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:No investigation of amrubicin monotherapy in pre-treated advanced non-small cell lung cancer (NSCLC) patients has yet been reported. PATIENTS AND METHODS:The records were reviewed of NSCLC patients who had received amrubicin monotherapy between 2003 and 2007 with the following eligibility criteria:previousl...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is expressed by immune cells and has been shown to play an important role in tumor surveillance due to its ability to induce apoptosis in various transformed cells. Interferon gamma (IFN-gamma), a multipotent cytokine with broad stimulatory effe...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00