Abstract:
BACKGROUND/AIM:The mechanism responsible for B-cell translocation gene 1 (BTG1) down-regulation in breast carcinoma remains unknown. We examined the BTG1 expression status in breast carcinoma cells and investigated the mechanism underlying the observed alterations. MATERIALS AND METHODS:Four breast carcinoma cell lines (SK-BR-3, MDA-MB-231, T-47D, and MCF-7), and one normal mammary epithelial cell line (MCF-10A) were analyzed. BTG1 expression was examined using quantitative reverse transcription polymerase chain reaction (PCR) and western blot. Methylation status of the BTG1 promoter was analyzed using methylation-specific PCR (MSP). To investigate the effect of methylation on BTG1, the cells were treated with a demethylating agent. RESULTS:The carcinoma cells expressed significantly lower levels of BTG1 mRNA and protein than normal cells. The BTG1 promoter was highly methylated in the carcinoma cells. 5-aza-2-deoxycytidine significantly restored BTG1 expression. CONCLUSION:Down-regulation of BTG1 expression through epigenetic repression is involved in mammary carcinogenesis. BTG1 is a potential diagnostic marker and therapeutic target for breast carcinoma.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Woo HY,DO SI,Kim SH,Song SY,Kim HSdoi
10.21873/anticanres.13729subject
Has Abstractpub_date
2019-10-01 00:00:00pages
5361-5367issue
10eissn
0250-7005issn
1791-7530pii
39/10/5361journal_volume
39pub_type
杂志文章abstract:BACKGROUND:TAOS1 (tumor amplified and overexpressed sequence 1) was recently cloned and found to be possibly important in driving the amplification of 11q13 in oral squamous cell carcinoma (SCC). MATERIALS AND METHODS:Quantitative RT-PCR was performed to determine the possible relationship between TAOS1 gene expressio...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Evidence from our laboratory suggests that conventional chromosome banding analysis alone is not sufficient for detecting interspecies contamination of cell lines. To differentiate noncycling interphase cells of murine or other species origin that may be contaminating a human cell line, molecular cytogenetic technique...
journal_title:Anticancer research
pub_type: 杂志文章
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abstract::Triple-negative breast cancer phenotype (estrogen receptor-, progesterone receptor- and human epidermal growth receptor 2-negative) is one of the most aggressive molecular subtypes, accounting for 15-20% of all breast tumors. There is no standard treatment for this setting of patients except anthracyclines and taxanes...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-08-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11038
更新日期:2016-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11803
更新日期:2017-08-01 00:00:00
abstract::The influence of human stromal fibroblasts cultured from normal, non-malignant and malignant breast tissues on in vivo growth of human breast tumour cells was studied. Earlier appearance and increased incidence were observed when low cell inocula of breast MDA-MB 231 tumour cells were coinjected with fibroblasts from ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::The relationship between human papillomavirus (HPV) infection and breast cancer is controversial. In this study, paraffin-embedded tissue blocks prepared from 72 patients with benign, premalignant or malignant mammary lesions were randomly collected from the Shanghai region of China and Tokushima in Japan. DNA specime...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
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更新日期:2016-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13079
更新日期:2019-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::Human urothelial cell lines of non-malignant and malignant origin were classified into three grades of transformation, i.e. TGr I, TGr II, and TGr III, which differ from primary and early cultures of normal bladder mucosa (TGr 0) by growth in the absence of fibroblasts, prolonged survival, increasing chromosomal abnor...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-02-01 00:00:00
abstract::Therapy of patients with metastatic renal cell carcinoma (mRCC) requires sequential use of several agents with different mechanisms and minimal cross-resistance between the different agents. Tyrosine kinase inhibitors (TKIs) and mammalian target of rapamycin (mTOR) inhibitors prolong progression-free survival (PFS) in...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2005-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Two human herpesviruses, HHV-6 and HHV-7, recently identified and closely related, were studied for their influence on cellular apoptosis and proliferation. Infection was monitored by viral DNA--and antigen expression. Apoptosis and cell proliferation were determined by immunocytological techniques and the markers p53...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND:In previous reports, changes in oxytocinase activity in human breast cancer tissue and in the serum of N-methyl-nitrosourea (NMU)-induced rat mammary tumors were described. Insulin-regulated aminopeptidase (IRAP) has been identified with oxytocinase and has also been referred to as placental leucine aminopep...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:Pleomorphic rhabdomyosarcomas of the uterus, mainly occurring in postmenopausal women with leading symptoms of vaginal bleeding and abdominal pain, are very rare malignant tumors of the female genital tract. Due to the inefficiency of the adjuvant therapy, the outcome remains poor in the majority of the repo...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:10.21873/anticanres.11592
更新日期:2017-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13870
更新日期:2019-12-01 00:00:00
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journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2010-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00