Abstract:
:The 4-quinolone antibiotics nalidixic acid and ciprofloxacin are potent inhibitors of the bacterial type II topoisomerase DNA gyrase. Treatment of mouse L1210 leukemia cells with these drugs resulted in a delayed inhibition of cell proliferation. Prior to inhibition of cell proliferation, there was a time-dependent decrease in the cellular content of mitochondrial DNA (mtDNA). The decrease in mtDNA was associated with a decrease in the rate of mitochondrial respiration and an increase in the concentration of lactate in the growth medium. Inhibition of cell proliferation by 4-quinolones was reversible upon drug washout. However, there was a 2- to 4-day lag before the growth rate returned to normal levels. This was preceded by an increase in mtDNA content and mitochondrial respiration. These studies suggest that inhibition of mammalian cell proliferation by 4-quinolone drugs is related to the selective depletion of mtDNA.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Lawrence JW,Darkin-Rattray S,Xie F,Neims AH,Rowe TCdoi
10.1002/jcb.240510208subject
Has Abstractpub_date
1993-02-01 00:00:00pages
165-74issue
2eissn
0730-2312issn
1097-4644journal_volume
51pub_type
杂志文章abstract::Understanding mechanisms that govern cell fate decisions will lead to developing techniques for induction of adult stem cell differentiation to desired cell outcomes and, thus, production of an autologos source of cells for regenerative medicine. Recently, we demonstrated that stem cells derived from adult central ner...
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journal_title:Journal of cellular biochemistry
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更新日期:2006-04-15 00:00:00
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