Intracellular proteolytic activity of cathepsin B is associated with capillary-like tube formation by endothelial cells in vitro.


:The lysosomal cysteine protease cathepsin B is implicated in degradation of extracellular matrix (ECM), a crucial step in a variety of physiological and pathological processes, including tumor dissemination and angiogenesis. In this study, we analyzed the contribution of extracellular and intracellular cathepsin B activity on the formation of capillary-like tubular structures by human umbilical vein endothelial cells (HUVECs) grown on Matrigel matrix, using general and specific cysteine protease inhibitors. We demonstrated, by confocal assay using quenched fluorescent protein substrate DQ-collagen IV, that endothelial cells degrade ECM both intracellularly and pericellularly. Intracellular cathepsin B activity detected by degradation of Z-Arg-Arg cresyl violet substrate was co-localized with the products of DQ-collagen IV degradation in the perinuclear region and in the capillary-like tubular structures. Treatment of cells with membrane-permeable CA-074 Me effectively abolished intracellular cathepsin B activity, and resulted in reduced tube length (32.3+/-9.4% at 10 microM), total tubule area (49.6+/-12.4% at 10 microM), and the number of branch points of tubules (47.5+/-7.7% at 10 microM) in a dose-dependent manner. In contrast, CA-074 (0.1-10 microM), a membrane-impermeable cathepsin B specific inhibitor, general cysteine protease inhibitors chicken cystatin (5 microM) and E-64 (10 microM), and the metalloprotease inhibitor Minocycline (10 microM) showed no significant inhibitory effect in our angiogenesis model. These results show that, besides multiple regulatory molecules, intracellular cathepsin B also contributes to the neovascularization process and should be considered as a potential therapeutic target.


J Cell Biochem


Premzl A,Turk V,Kos J




Has Abstract


2006-04-15 00:00:00












  • Constitutive and inducible expression of cytochromes P4501A (CYP1A1 and CYP1A2) in normal prostate and prostate cancer cells.

    abstract::Constitutive and benzo[a]pyrene (B[a]P) inducible expression of CYP1A1 and CYP1A2 in prostate cancer and normal prostate epithelial cells were examined by immunoblotting. Androgen independent prostate cancer cell lines DU145 and PC3 have constitutive expression of CYP1A and CYP1A1 and CYP1A2, respectively. Four microm...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Sterling KM Jr,Cutroneo KR

    更新日期:2004-02-01 00:00:00

  • Biological evidence of the relationship between Helicobacter pylori and associated extragastric diseases.

    abstract:BACKGROUND:Gram-negative bacterium, Helicobacter pylori is notorious for various pathologies like peptic ulcers, gastritis, functional dyspepsia, and various cancers. METHODS:Systemic effects of its toxins have led scientists' attention toward the extragastric pathologies associated with it. To date, it has been shown...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Zendehdel A,Roham M

    更新日期:2019-08-01 00:00:00

  • Phosphorylation of the insulin receptor by casein kinase I.

    abstract::Insulin receptor was examined as a substrate for the multipotential protein kinase casein kinase I. Casein kinase I phosphorylated partially purified insulin receptor from human placenta as shown by immunoprecipitation of the complex with antiserum to the insulin receptor. Analysis of the phosphorylated complex by pol...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Tuazon PT,Pang DT,Shafer JA,Traugh JA

    更新日期:1985-01-01 00:00:00

  • Fullerene-polyvinylpyrrolidone clathrate localizes in the cytoplasm to prevent Ultraviolet-A ray-induced DNA-fragmentation and activation of the transcriptional factor NF-kappaB.

    abstract::By Western blot and immunostaining we proved that polyvinylpyrrolidone (PVP)-wrapped fullerene molecules (PVP-fullerene) could combine the 8- and 53-kb proteins which localize in the membrane of human skin keratinocytes HaCaT. Only fullerene molecules are able to cross the lipid membrane and conjugate 53-kb proteins i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Xiao L,Aoshima H,Saitoh Y,Miwa N

    更新日期:2010-11-01 00:00:00

  • Overexpression of the long noncoding RNA TRHDE-AS1 inhibits the progression of lung cancer via the miRNA-103/KLF4 axis.

    abstract:AIM:The aim of this study was to explore the role and molecular basis of the long noncoding RNA (lncRNA) TRHDE-AS1 in lung cancer. METHODS:We used real-time polymerase chain reaction to analyze the messenger RNA expression levels of TRHDE-AS1, miR-103, and KLF4. The cell viability, proliferation, and invasion rates we...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zhuan B,Lu Y,Chen Q,Zhao X,Li P,Yuan Q,Yang Z

    更新日期:2019-10-01 00:00:00

  • Novel identification of peripheral dopaminergic D2 receptor in male germ cells.

    abstract::Dopamine is a recognized modulator in the central nervous system (CNS) and peripheral organ functions. The presence of peripheral dopamine receptors outside the CNS has suggested an intriguing interaction between the nervous system and other functional systems, such as the reproductive system. In the present study we ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Otth C,Torres M,Ramírez A,Fernandez JC,Castro M,Rauch MC,Brito M,Yañez AJ,Rodríguez-Gil JE,Slebe JC,Concha II

    更新日期:2007-01-01 00:00:00

  • Heparan sulfate proteoglycans as trastuzumab targets in anoikis-resistant endothelial cells.

    abstract::Anoikis is a form of programmed cell death induced by loss of contact from neighboring cells or from their extracellular matrix (ECM). Many tumorigenic cells are anoikis resistant, facilitating cancer progression and metastasis. Trastuzumab is a monoclonal antibody used for the treatment of breast and gastric cell can...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Onyeisi JOS,Castanho de Almeida Pernambuco Filho P,de Araujo Lopes S,Nader HB,Lopes CC

    更新日期:2019-08-01 00:00:00

  • High glucose condition suppresses neurosphere formation by human periodontal ligament-derived mesenchymal stem cells.

    abstract::D-Glucose serves many roles in cellular functions, but its role in human periodontal ligament-derived mesenchymal stem cells (hPSLSCs) is yet unknown. Here, the roles of high glucose concentration on neurogenic differentiation by hPDLSCs were investigated. Two-stage neurogenic induction protocol was employed. Cells we...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Sawangmake C,Pavasant P,Chansiripornchai P,Osathanon T

    更新日期:2014-05-01 00:00:00

  • Deletion of insulin receptor in the proximal tubule and fasting augment albumin excretion.

    abstract::The contribution of proximal tubules (PT) to albumin uptake is now well recognized, however, its regulation is understudied area. There are reports suggesting that insulin resistance is associated with the development of albuminuria in nondiabetic individuals. We have previously reported reduced insulin receptor (IR) ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Kumari M,Sharma R,Pandey G,Ecelbarger CM,Mishra P,Tiwari S

    更新日期:2019-06-01 00:00:00

  • Interactions between muscle tissues and bone metabolism.

    abstract::Sarcopenia and osteoporosis have recently been noted for their relationship with locomotive syndrome and increased number of older people. Sarcopenia is defined by decreased muscle mass and impaired muscle function, which may be associated with frailty. Several clinical data have indicated that increased muscle mass i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Kawao N,Kaji H

    更新日期:2015-05-01 00:00:00

  • Dissecting the roles of individual interactions in protein stability: lessons from a circularized protein.

    abstract::A circular form of bovine pancreatic trypsin inhibitor (BPTI) has been prepared by introducing a peptide bond between the N- and C-termini, which are in close proximity in the native conformation. The pathway and energetics of the disulphide-coupled folding transition of the circular protein have been studied using me...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Goldenberg DP

    更新日期:1985-01-01 00:00:00

  • The role of regulatory components from resident T lymphocytes in polyclonal B cell activation.

    abstract::Resident T lymphocytes have been found to exert helper and suppressor regulatory influences with regard to polyclonal activation of murine splenic B lymphocytes elicited by lipopolysaccharide. In the normal adult spleen, only T cell helper influences are exercised over polyclonal B cell activation. This activity is a ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Goodman MG,Weigle WO

    更新日期:1982-01-01 00:00:00

  • Osteoblasts protect AML cells from SDF-1-induced apoptosis.

    abstract::The bone marrow provides a protective environment for acute myeloid leukemia (AML) cells that often allows leukemic stem cells to survive standard chemotherapeutic regimens. Targeting these leukemic stem cells within the bone marrow is critical for preventing relapse. We recently demonstrated that SDF-1, a chemokine a...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Kremer KN,Dudakovic A,McGee-Lawrence ME,Philips RL,Hess AD,Smith BD,van Wijnen AJ,Karp JE,Kaufmann SH,Westendorf JJ,Hedin KE

    更新日期:2014-06-01 00:00:00

  • The effect of long noncoding RNAs HOX transcript antisense intergenic RNA single-nucleotide polymorphisms on breast cancer, cervical cancer, and ovarian cancer susceptibility: A meta-analysis.

    abstract::Recent studies have shown that long noncoding RNAs (lncRNA) HOX transcript antisense intergenic RNA (HOTAIR) polymorphisms are associated with cancer susceptibility. The greatest threat to women's health among a variety of cancers is breast cancer (BC), cervical cancer (CC), and ovarian cancer (OC), and the incidence ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Li J,Liu R,Tang S,Feng F,Wang X,Qi L,Liu C,Yao Y,Sun C

    更新日期:2018-11-28 00:00:00

  • Osteoprotegerin inhibit osteoclast differentiation and bone resorption by enhancing autophagy via AMPK/mTOR/p70S6K signaling pathway in vitro.

    abstract::Osteoclasts are highly differentiated terminal cells formed by fusion of hematopoietic stem cells. Previously, osteoprotegerin (OPG) inhibit osteoclast differentiation and bone resorption by blocking receptor activator of nuclear factor-κB ligand (RANKL) binding to RANK indirect mechanism. Furthermore, autophagy plays...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Tong X,Gu J,Song R,Wang D,Sun Z,Sui C,Zhang C,Liu X,Bian J,Liu Z

    更新日期:2018-09-06 00:00:00

  • Role of angiotensin-converting enzyme insertion/deletion polymorphism in sudden cardiac arrest.

    abstract:OBJECTIVE:This study aimed to investigate the relationship between angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism and to understand sudden cardiac arrest (SCA) in the Chinese population. METHODS:In this study, 232 patients were divided into the SCA group and the coronary disease group with c...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Liu A,Wang S,Zhang C,Sun D,Song X

    更新日期:2019-03-01 00:00:00

  • In Vitro Antitumor Effects of AHR Ligands Aminoflavone (AFP 464) and Benzothiazole (5F 203) in Human Renal Carcinoma Cells.

    abstract::We investigated activity and mechanism of action of two AhR ligand antitumor agents, AFP 464 and 5F 203 on human renal cancer cells, specifically examining their effects on cell cycle progression, apoptosis, and migration. TK-10, SN12C, Caki-1, and ACHN human renal cancer cell lines were treated with AFP 464 and 5F 20...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Luzzani GA,Callero MA,Kuruppu AI,Trapani V,Flumian C,Todaro L,Bradshaw TD,Loaiza Perez AI

    更新日期:2017-12-01 00:00:00

  • TE2 and TE1 sub-elements of the testis-specific histone H1t promoter are functionally different.

    abstract::The testis-specific linker histone H1t gene is transcribed exclusively in pachytene primary spermatocytes. Tissue specific expression of the gene is mediated in part by transcriptional factors that bind elements located within the proximal and distal promoter. A 40 bp promoter element, designated H1t/TE, that is locat...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Wilkerson DC,Wolfe SA,Grimes SR

    更新日期:2003-04-15 00:00:00

  • Jedi--a novel transmembrane protein expressed in early hematopoietic cells.

    abstract::Self-renewal and differentiation of hematopoietic stem and progenitor cells are defined by the ensembles of genes expressed by these cells. Here we report identification of a novel gene named Jedi, which is expressed predominantly in short- and long-term repopulating stem cells when compared to more mature bone marrow...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Krivtsov AV,Rozov FN,Zinovyeva MV,Hendrikx PJ,Jiang Y,Visser JW,Belyavsky AV

    更新日期:2007-06-01 00:00:00

  • cAMP-dependent protein kinase modulates expression and subcellular localization of Dolichos biflorus agglutinin binding sites in renal epithelial cells.

    abstract::Previous studies demonstrated that, upon attaining confluence, a clone of the renal epithelial cell, LLC-PK1, expressed progressively binding sites for the lectin Dolichos biflorus agglutinin (DBA) at the apical cell surface. Activation of cAMP-dependent protein kinase enhanced surface expression dramatically. The goa...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Amsler K

    更新日期:1993-08-01 00:00:00

  • Effects of human placenta-derived mesenchymal stem cells with NK4 gene expression on glioblastoma multiforme cell lines.

    abstract::Poor prognosis and low survival are commonly seen in patients with glioblastoma multiforme (GBM). Due to the specific nature of solid tumors such as GBM, delivery of therapeutic agents to the tumor sites is difficult. So, one of the major challenges in the treatment of these tumors is a selection of appropriate method...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Jabbarpour Z,Kiani J,Keshtkar S,Saidijam M,Ghahremani MH,Ahmadbeigi N

    更新日期:2020-02-01 00:00:00

  • Suppression of EC-SOD by oxLDL During Vascular Smooth Muscle Cell Proliferation.

    abstract::Reactive oxygen species (ROS) produced by endothelial cells and macrophages play important roles in atherogenesis because they promote the formation of oxidized low-density lipoproteins (oxLDL). Extracellular-superoxide dismutase (EC-SOD) is mainly produced by vascular smooth muscle cells (VSMCs), is secreted into the...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Makino J,Asai R,Hashimoto M,Kamiya T,Hara H,Ninomiya M,Koketsu M,Adachi T

    更新日期:2016-11-01 00:00:00

  • Tributyltin oxide exposure impairs mouse oocyte maturation and its possible mechanisms.

    abstract::Tributyltin oxide (TBTO) has been widely used as marine antifouling composition, preservative, biocide, and a stabilizer in plastic industry. Previous studies have indicated that TBTO can cause immunotoxicity as an environmental pollutant. However, little is known about its reproductive toxicity, especially on female ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yang LL,Cui YX,Ma JY,Ge ZJ,Shen W,Yin S

    更新日期:2019-01-01 00:00:00

  • Src protein and tyrosine-phosphorylated protein profiles in marrow stroma during osteogenic stimulation.

    abstract::Src protein is essential for the regulation of bone turnover primarily via bone resorption because it is required in osteoclast differentiation and function. We followed temporal changes of Src protein abundance in marrow stromal cells induced to mineralize by dexamethasone (DEX), growth in cold temperature, or both. ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Klein BY,Levitzki R,Ben-Bassat H

    更新日期:1998-06-01 00:00:00

  • Regulation by glucocorticoids of cell differentiation and insulin-like growth factor binding protein production in cultured fetal rat nasal chondrocytes.

    abstract::Glucocorticoids (GCs) modulate insulin-like growth factor action in cartilage through mechanisms that are complex and insufficiently defined, especially in the context of cranio-facial growth. Because the family of IGF-binding proteins (IGFBP-1 to -6) is important in the regulation of IGF availability and bioactivity,...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Nadra R,Menuelle P,Chevallier S,Berdal A

    更新日期:2003-04-01 00:00:00

  • Mechanisms regulating c-met overexpression in liver-metastatic B16-LS9 melanoma cells.

    abstract::Liver selected B16-LS9 melanoma cells show a dramatic overexpression of the proto-oncogene c-met, the cellular receptor for hepatocyte growth factor/scatter factor. As a consequence, c-met becomes constitutively active, and the cells become more responsive to hepatocyte growth factor stimulation. We have investigated ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Elia G,Ren Y,Lorenzoni P,Zarnegar R,Burger MM,Rusciano D

    更新日期:2001-01-01 00:00:00

  • Tissue crosstalk in lung development.

    abstract::Lung development follows a stereotypic program orchestrated by key interactions among epithelial and mesenchymal tissues. Deviations from this developmental program can lead to pulmonary diseases including bronchopulmonary dysplasia and pulmonary hypertension. Significant efforts have been made to examine the cellular...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Hines EA,Sun X

    更新日期:2014-09-01 00:00:00

  • miR-21 downregulates the tumor suppressor P12 CDK2AP1 and stimulates cell proliferation and invasion.

    abstract::The present study was undertaken to investigate the regulation of P12(CDK2AP1) by miRNAs. A conserved target site for miR-21 within the CDK2AP1-3'-UTR at nt 349-370 was predicted by bioinformatics software and an inverse correlation of miR-21 and CDK2AP1 protein was observed. Highly specific amplification and quantifi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zheng J,Xue H,Wang T,Jiang Y,Liu B,Li J,Liu Y,Wang W,Zhang B,Sun M

    更新日期:2011-03-01 00:00:00

  • Membrane signalling and progesterone in female and male osteoblasts. I. Involvement Of intracellular Ca(2+), inositol trisphosphate, and diacylglycerol, but not cAMP.

    abstract::Bone is a target tissue of progestins, but the mechanisms by which they act are still unclear. We examined the early (5-60 s) effects of progesterone and progesterone covalently bound to BSA (P-CMO BSA), which does not enter the cell, on the cytosolic free Ca(2+) concentration ([Ca(2+)]i) and the formation of inositol...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Grosse B,Kachkache M,Le Mellay V,Lieberherr M

    更新日期:2000-08-02 00:00:00

  • Microarray profiling analysis identifies the mechanism of miR-200b-3p/mRNA-CD36 affecting diabetic cardiomyopathy via peroxisome proliferator activated receptor-γ signaling pathway.

    abstract:OBJECTIVE:Current study focused on the influence of miR-200b-3p on cardiocyte apoptosis of diabetic cardiomyopathy (DCM) by regulating CD36 and peroxisome proliferator-activated receptor γ (PPAR-γ) signaling pathway. METHODS:Bioinformatic analysis was used to analyze differentially expressed microRNA (miRNAs), messeng...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Xu L,Chen W,Ma M,Chen A,Tang C,Zhang C,Cai L

    更新日期:2019-04-01 00:00:00