Abstract:
:As an extension of our recently published work (Mlejnek and Kuglík [2000] J. Cell. Biochem. 77:6-17), the role of caspases in N(6)-benzylaminopurine riboside (BAPR)-induced apotosis in HL-60 cells was evaluated in this study. Here, BAPR-induced apoptosis was accompanied by activation of caspase-3 and caspase-9. However, when these caspases were selectively inhibited, the progression of BAPR-induced apoptosis was not markedly affected. Besides that, activation of caspase-3 and caspase-9 was found to be rather late event in apoptotic process. These results suggested that other caspases might be critically implicated. Indeed, pan-specific caspase inhibitor, Z-VAD-FMK, completely prevented DNA cleavage and apoptotic bodies formation. However, Z-VAD-FMK failed to prevent cell death and it was incapable to fully counteract the main apoptotic hallmark-chromatin condensation. Finally, our data indicate that cellular decision between apoptosis and necrosis is made upon the availability of both caspase proteases and intracellular ATP.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Mlejnek Pdoi
10.1002/jcb.1262subject
Has Abstractpub_date
2001-01-01 00:00:00pages
678-89issue
4eissn
0730-2312issn
1097-4644pii
10.1002/jcb.1262journal_volume
83pub_type
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abstract:OBJECTIVE:The aim of the study was to explore the mechanism of excessive apoptosis of nucleus pulposus cells induced by short hairpin RNA (shRNA) Piezo type mechanosensitive ion channel component 1 (Piezo1) under abnormal mechanical stretch stress. METHODS:In vitro mechanical stretch stress model of nucleus pulposus c...
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更新日期:2019-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00