Abstract:
:A murine model of allergic bronchopulmonary aspergillosis (ABPA), developed by exposure to Aspergillus fumigatus antigens, demonstrated eosinophilia of peripheral blood (PB), bone marrow (BM), and lung. The eosinophilia was abrogated by monoclonal anti-interleukin-5 (IL-5) antibody (TRFK-5) and not by an isotype control antibody (GL 113). Eosinophils in PB were enumerated from stained smears and their relative increase or decrease in cells from BM and lung was determined by an eosinophil peroxidase (EPO) assay (measured in optical density). Intraperitoneal injection of TRFK-5 in mice exposed to A. fumigatus antigen produced a significant reduction in eosinophils (PB 6.6 +/- 1.14% vs. 3.8 +/- 0.8%, P < .01) and EPO production in BM (0.935 +/- 0.03 vs. 0.615 +/- 0.02, P < .001). A similar reduction in EPO production in the lung (0.691 +/- 0.12 vs. 0.495 +/- 0.05, not significant) was also reflected in the histopathology for the different groups of mice. These findings confirming the role of IL-5 in eosinophilia, although not surprising, are significant in elucidating the immunopathogenesis of ABPA in the murine model. We conclude that in this model, eosinophilia may be due largely to the Th2 cytokine -IL-5 induced by A. fumigatus antigens.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Murali PS,Kumar A,Choi H,Banasal NK,Fink JN,Kurup VPdoi
10.1002/jlb.53.3.264subject
Has Abstractpub_date
1993-03-01 00:00:00pages
264-7issue
3eissn
0741-5400issn
1938-3673journal_volume
53pub_type
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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abstract::TNF is implicated in the suppression of neutrophil apoptosis during sepsis. Multiple signaling pathways are involved in TNF-mediated antiapoptotic signaling; a role for the MAP kinases (MAPK), ERK1/2, and p38 MAPK has been suggested. Antiapoptotic signaling is mediated principally through TNF receptor-1 (TNFR-1), and ...
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.3A0915-393R
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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abstract::Rat thymocytes respond to exposure to phytomitogens by oxidant generation as detected by chemiluminescence in presence of luminol. Maximal chemiluminescent response to a wide variety of plant lectins was obtained only after the cells had been incubated for 18 hours at 37 degrees C but not at 4 degrees C. This temperat...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.52.5.545
更新日期:1992-11-01 00:00:00
abstract::The specific binding of human polymorphonuclear leukocytes (PMN) to antibody-coated target cells was characterized by flow cytometry. PMN were labeled with phycoerythrin-E (PE) via a granulocyte-specific monoclonal antibody (leu-M1) and mixed with fluorescein isothiocyanate-labeled K562 tumor cells sensitized with rab...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.52.4.390
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.53.5.550
更新日期:1993-05-01 00:00:00
abstract::Polyamines naturally occur in all living beings and play an important role in the regulation of cell proliferation, differentiation, and functional stimulation of terminally differentiated cells. Our studies, using specific inhibitors of polyamine biosynthesis such as alpha-difluoromethylornithine and methylglyoxal-bi...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.52.6.585
更新日期:1992-12-01 00:00:00
abstract::As the natural resistance-associated macrophage protein 1 Nramp1 (also known as Slc11a1) modulates Kupffer cell (KC) activation, and KC are responsible for the early phase of warm ischemia/reperfusion (I/R) to the liver, we hypothesized that livers of Nramp1(-/-) mice will be protected from early-phase I/R injury comp...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
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abstract::Neonatal CD71+ erythroid cells are thought to have immunosuppressive functions. Recently, we demonstrated that CD71+ erythroid cells from neonates born to women who underwent spontaneous preterm labor (PTL) are reduced to levels similar to those of term neonates; yet, their functional properties are unknown. Herein, w...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
abstract::A simple and rapid method for the purification of morphologically and functionally intact eosinophils from human blood of both normal and eosinophilic subjects is described. The method is based on a single centrifugation of total blood leukocytes suspended in Percoll with specific gravity 1.0853 g/ml, after erythrocyt...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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更新日期:1992-09-01 00:00:00
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journal_title:Journal of leukocyte biology
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更新日期:1995-03-01 00:00:00
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
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更新日期:2004-01-01 00:00:00
abstract::In this study, we examined the expression of nerve growth factor (NGF) and its receptors in mouse macrophages and the mechanisms involved in the effect of NGF on tumor necrosis factor (TNF)-alpha production. Macrophages expressed NGF and the NGF receptors TrkA and p75. Treatment of J744 cells or peritoneal macrophages...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00