Fibronectin patterns in hepatocellular carcinoma and its clinical significance.

Abstract:

:Fibronectin, a high molecular weight glycoprotein component of the extracellular matrix, was localized extracellularly and intracellularly in the nuclei and the cytoplasm of hepatocellular carcinomas (HCC) by the use of the biotin-avidin method on paraffin-embedded, pepsin-treated tissue. The specificity of the staining was confirmed by absorbing the antibodies with purified fibronectin. Twenty HCC were investigated (7 were autopsy specimens and 13 biopsy and surgical specimens). The fibronectin was either increased or decreased. When increased the fibronectin consisted of thin or thick feathery strands and was either pericellular, sinusoidal, periglandular, or stromal. Marked increase of fibronectin was seen in fibrolamellar HCC, clear cell HCC, and encapsulated HCC, correlating with the better prognosis. Decreased fibronectin was seen in two undifferentiated HCC. HCC obtained at autopsy displayed nuclear and cytoplasmic fibronectin staining. Nuclear fibronectin may be an expression of the DNA binding ability of fibronectin which becomes demonstrable in cells with increased membrane permeability.

journal_name

Cancer

journal_title

Cancer

authors

Jagirdar J,Ishak KG,Colombo M,Brambilla C,Paronetto F

doi

10.1002/1097-0142(19851001)56:7<1643::aid-cncr2820

subject

Has Abstract

pub_date

1985-10-01 00:00:00

pages

1643-8

issue

7

eissn

0008-543X

issn

1097-0142

journal_volume

56

pub_type

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