Bacterial adherence and biofilm formation on latex and silicone T-tubes in relation to bacterial contamination of bile.

Abstract:

BACKGROUND:T-tube-related bacteriobilia causes infectious complications and obstruction. To prevent these, the choice of T-tube material may be of importance. METHODS:Transected common bile ducts (CBDs) of 17 piglets were sutured over latex or silicone T-tubes, or without a T-tube. RESULTS:After 6 weeks bacteriobilia was found in all of 12 CBDs with and in 1 of 5 CBDs without a T-tube (p < 0.05). By scanning electron microscopy (SEM) four of five latex and none of five silicone T-tubes had bacterial biofilms (p < 0.05). All tubes remained patent. Segments of T-tubes were incubated with five different bacterial strains. Sonication and SEM showed that 0.1-1.1% of 10(7) colony-forming units of inoculum adhered to T-tubes. Two to six times more bacteria adhered to latex than to silicone (p < 0.05). CONCLUSIONS:Silicone offers better long-term patency than latex. Less infectious complications occur if T-tubes are omitted.

journal_name

Scand J Gastroenterol

authors

Koivusalo A,Mäkisalo H,Talja M,Siitonen A,Vuopio-Varkila J,Ruutu M,Höckerstedt K

doi

10.3109/00365529609006417

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

398-403

issue

4

eissn

0036-5521

issn

1502-7708

journal_volume

31

pub_type

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