Validation of microdialysis probes for studying nitrosative chemistry within localized regions of the human upper gastrointestinal tract.

Abstract:

BACKGROUND:We have examined the suitability of microdialysis probes for examining nitrosative chemistry within localized regions of the lumen of the gastrointestinal tract. Chemical nitrosation occurs maximally at pH 2.5 in the presence of nitrite and thiocyanate and absence of ascorbic acid. Nitrite and thiocyanate are delivered into the stomach in saliva and ascorbic acid is secreted in gastric juice. METHODS:We used a benchtop model to reproduce the nitrosative chemistry occurring in the human upper GI tract and assessed the ability of the microdialysis probes to measure it. RESULTS:The microdialysis probes were reliable at measuring nitrite ascorbic acid, total vitamin C and thiocyanate in both aqueous solutions and human gastric juice over the full range of intragastric pH, i.e. 1.5, 2.5, 3.5, 5.0 and 7.0. The probes were also reliable at measuring these chemicals under conditions simulating the active interaction between nitrite and ascorbic acid. Under such conditions with ascorbic acid in excess the probes gave a more accurate assessment of the nitrite level than that obtained by directly sampling the gastric juice. This was due to the probes not being subject to artefactual measurement of nitric oxide as nitrite. This was prevented by the rapid diffusion of nitric oxide through the probe collecting tube. CONCLUSION:Microdialysis probes provide a reliable means of examining nitrosative chemistry within the lumen of the upper GI tract. In addition, they have the advantage of measuring this chemistry in very local regions and of simultaneously comparing the chemistry in different regions of the upper GI tract.

journal_name

Scand J Gastroenterol

authors

Suzuki H,Moriya A,Iijima K,McElroy K,Fyfe VE,McColl KE

doi

10.1080/00365520310004119

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

856-63

issue

8

eissn

0036-5521

issn

1502-7708

journal_volume

38

pub_type

杂志文章
  • Gastrointestinal bleeding due to peptic ulcers and erosions - a prospective observational study (BLUE study).

    abstract:OBJECTIVES:Acute upper gastrointestinal bleeding is a well-recognized complication of peptic ulcers and erosions. The aim of this study was to assess the incidence rate and identify risk factors for this complication in southeastern Norway. MATERIALS AND METHODS:Between March 2015 and December 2017, a prospective obse...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2020.1819405

    authors: Romstad KK,Detlie TE,Søberg T,Ricanek P,Jahnsen ME,Lerang F,Jahnsen J

    更新日期:2020-10-01 00:00:00

  • Adipokine profile in celiac patients: differences in comparison with patients suffering from diarrhea-predominant IBS and healthy subjects.

    abstract::OBJECTIVE. The role of adipokines such as resistin, leptin, and adiponectin could be pivotal in the molecular crosstalk between the inflamed intestine and the surrounding mesenteric adipose tissue. Our aims were to a) evaluate their circulating concentrations in patients with active celiac disease (ACD) and compare th...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.3109/00365521.2013.845907

    authors: Russo F,Chimienti G,Clemente C,D'Attoma B,Linsalata M,Orlando A,De Carne M,Cariola F,Semeraro FP,Pepe G,Riezzo G

    更新日期:2013-12-01 00:00:00

  • Morphology in the cat ileal mucosa following construction of an ileal reservoir or transposition of patches to different locations.

    abstract::In an attempt to examine the response of the ileal mucosa to different noxious environments and to correlate the results with findings in human continent ileostomy reservoirs, cat intestine was subjected to various operative procedures: first, ileal reservoirs were constructed in continuity with the small intestine, a...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:

    authors: Philipson B

    更新日期:1975-01-01 00:00:00

  • Apoptosis, proliferation and differentiation patterns are influenced by Zebularine and SAHA in pancreatic cancer models.

    abstract:OBJECTIVE:Pancreatic cancer continues to be an urgent clinical problem. We used the novel DNA methyltransferase inhibitor Zebularine and the histone deacetylase inhibitor SAHA to investigate the epigenetic influence on viability and differentiation of the pancreatic cancer cell lines YAP C, DAN G and Panc-89 in vitro a...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520600874198

    authors: Neureiter D,Zopf S,Leu T,Dietze O,Hauser-Kronberger C,Hahn EG,Herold C,Ocker M

    更新日期:2007-01-01 00:00:00

  • Detection of Helicobacter pylori gene by means of immunomagnetic separation-based polymerase chain reaction in feces.

    abstract:BACKGROUND:Detection of Helicobacter pylori is usually performed by culture, polymerase chain reaction (PCR), histology, or urease test on gastric biopsy samples. Although methods based on feces are non-invasive, their sensitivity has been relatively low. In this study, to improve its sensitivity, immunomagnetic separa...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365529850172476

    authors: Watanabe T,Tomita S,Kudo M,Kurokawa M,Orino A,Todo A,Chiba T

    更新日期:1998-11-01 00:00:00

  • Spontaneous splenic rupture precipitated by cough: a case report and a review of the literature.

    abstract::Spontaneous rupture of a normal spleen without a history of trauma is a rare clinical entity. We report on a case of atraumatic splenic rupture in a 61-year-old man who presented to the emergency department for abdominal pain and hypotension. There was no evidence of hematologic or infectious disease involving the spl...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.1080/00365520701763472

    authors: Wehbe E,Raffi S,Osborne D

    更新日期:2008-01-01 00:00:00

  • Extracellular vesicle-associated soluble CD163 and CD206 in patients with acute and chronic inflammatory liver disease.

    abstract::Background: Extracellular vesicles (EVs) are implicated in intercellular communication in liver diseases. An EV-associated fraction of the macrophage biomarker soluble CD163, denoted EV-CD163, was recently identified. EV-CD163 may be released during later phases of the inflammatory response as opposed to the acute she...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2020.1759140

    authors: Nielsen MC,Andersen MN,Grønbæk H,Damgaard Sandahl T,Møller HJ

    更新日期:2020-05-01 00:00:00

  • Anal endosonography in asymptomatic subjects.

    abstract::The anal sphincter thickness was measured ultrasonographically in 30 asymptomatic subjects (13 male and 17 female: 23 young and 7 old). The internal anal sphincter (IAS) was measured at its thickest portion in the anal canal, its borders lying between the submucosa and the external and sphincter (EAS). The EAS was mea...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529309098265

    authors: Papachrysostomou M,Pye SD,Wild SR,Smith AN

    更新日期:1993-06-01 00:00:00

  • Release of vasoactive intestinal polypeptide (VIP) by intraduodenal stimuli.

    abstract::The effect of intraduodenal infusion of amino acids, glucose, fat, HCl, ethanol, or saline on plasma VIP concentration was investigated in 7 normal subjects, 5 post-vagotomy patients, and 12 anaesthetized pigs. Furthermore, the concentrations of VIP in plasma after ingestion of a mixed meal were measured in 6 normal s...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365527709181722

    authors: Schaffalitzky de Muckadell OB,Fahrenkrug J,Holst JJ,Lauritsen KB

    更新日期:1977-01-01 00:00:00

  • Impaired lipopolysaccharide-induced interleukin-1-beta production in patients with anti-hepatitis C virus antibody-positive chronic liver disease.

    abstract::The aim of our study was to determine the role of cytokine interleukin-1-beta (IL-1-beta) in patients with chronic hepatitis C virus (HCV) infection. Twenty-eight patients with chronic HCV infection were studied and compared with 18 healthy subjects. IL-1-beta levels were measured with an enzyme-linked immunosorbent a...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529409090477

    authors: Ozyilkan E,Tatar G,Hacibektaşoglu A,Kayhan B,Telatar H

    更新日期:1994-03-01 00:00:00

  • Transjugular intrahepatic portosystemic shunt placement for refractory ascites: a single-centre experience.

    abstract:BACKGROUND:The presence of refractory ascites is a common indication for transjugular intrahepatic portosystemic shunt (TIPS). Different models have been proposed for the prediction of survival after TIPS. The aim of this study was to evaluate the predictive factors associated with patients' survival after TIPS placeme...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2012.703239

    authors: Lodato F,Berzigotti A,Lisotti A,Azzaroli F,Mosconi C,Giampalma E,Renzulli M,Cappelli A,Buonfiglioli F,Calvanese C,Zoli M,Golfieri R,Mazzella G

    更新日期:2012-12-01 00:00:00

  • Helicobacter pylori in functional dyspepsia and normal controls.

    abstract:BACKGROUND:The aim of this study was to examine whether Helicobacter pylori status can identify a specific subset of patients with functional dyspepsia (FD) and erosive prepyloric changes (EPC). METHODS:Anamnestic, somatic, and psychologic data were collected from 87 patients with functional dyspepsia and 77 normal co...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529409092466

    authors: Wilhelmsen I,Tangen Haug T,Sipponen P,Berstad A

    更新日期:1994-06-01 00:00:00

  • The renin-angiotensin system in Barrett's esophagus.

    abstract:OBJECTIVE:Barrett's esophagus (BE) is a risk factor for esophageal adenocarcinoma. In addition to its classical endocrine character known for hemodynamic regulation, the renin-angiotensin system (RAS) can be associated with inflammation, wound healing, and cancer. The aim of this study was to explore a potential expres...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2016.1174881

    authors: Bratlie SO,Edebo A,Casselbrant A,Helander HF,Fändriks L

    更新日期:2016-09-01 00:00:00

  • Eosinophilic gastroenteritis: our recent experience: one-year experience of atypical onset of an uncommon disease.

    abstract::Eosinophilic gastroenteritis is an unusual entity that is rarely found in daily clinical practise. Its aetiology is unknown and diagnosis can be made microscopically with evidence of massive eosinophilic infiltration in patients with chronic gastrointestinal symptoms, excluding entities that may cause such findings (p...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655201317097254

    authors: Redondo-Cerezo E,Cabello MJ,González Y,Gómez M,García-Montero M,de Teresa J

    更新日期:2001-12-01 00:00:00

  • Urinary prostaglandins and renal function in chronic liver diseases.

    abstract::Changes in urinary PGE2 and PGF2 alpha excretion in chronic liver diseases were observed in relation to renal hemodynamics and sodium balance. After equilibration on a 110-170-meq sodium diet, daily urine collections were analyzed for PGE2 and PGF2 alpha by a new extraction and radioimmunoassay method. PGE2 was signif...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528609034626

    authors: Uemura M,Tsujii T,Fukui H,Kita K,Takaya A,Tsukamoto N,Nakayama M,Matsumoto M,Tamura M

    更新日期:1986-01-01 00:00:00

  • Invasive and non-invasive diagnosis of Helicobacter pylori-associated atrophic gastritis: a comparative study.

    abstract:OBJECTIVE:Helicobacter pylori-associated atrophic gastritis is known to be a significant risk factor for gastric cancer. Among the well-known parameters of atrophic gastritis are the levels of serum gastrin-17 (G-17) and pepsinogen I (PG1), which are biomarkers of gastric antral and corpus mucosal activity, respectivel...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520410010607

    authors: Pasechnikov VD,Chukov SZ,Kotelevets SM,Mostovov AN,Mernova VP,Polyakova MB

    更新日期:2005-03-01 00:00:00

  • Effects of insulin and beta-adrenergic blockade on the migrating motor complex in humans.

    abstract:BACKGROUND:Interdigestive small-intestinal motility is suppressed by hyperglycemia and also by hyperinsulinemia per se. Since hyperinsulinemia may increase sympathetic activity, the present study was undertaken to ascertain to what extent insulin affects phase III of the migrating motor complex (MMC) and MMC-related du...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529509093267

    authors: Björnsson ES,Urbanavicius V,Eliasson B,Attvall S,Smith U,Abrahamsson H

    更新日期:1995-03-01 00:00:00

  • The new prophylactic strategy for colon cancer in inflammatory bowel disease by modulating microbiota.

    abstract::It is well understood that intestinal microbiota play an important role in the pathogenesis of inflammatory bowel disease (IBD). In addition, IBD patients are well known to have a higher risk of developing colon cancer due to chronic inflammation. Recent evidence suggests that manipulation of microbiota improves the c...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章,评审

    doi:10.3109/00365521.2012.741617

    authors: Kanauchi O,Mitsuyama K,Andoh A

    更新日期:2013-04-01 00:00:00

  • The prognosis of chronic aggressive hepatitis. A clinical and morphological follow-up study.

    abstract::In a follow-up study of 85 patients with chronic aggressive (active) hepatitis (CAH) repeated liver biopsies and/or autopsy liver sections were available in 74 cases. The median time of observation was 45 months. Cirrhosis was demonstrated in 38 patients, and cirrhosis was suspected in a further five cases. Fifteen pa...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365527709180930

    authors: Dietrichson O,Christoffersen P

    更新日期:1977-01-01 00:00:00

  • Predictors of quality-of-life after ileal pouch-anal anastomosis in patients with ulcerative colitis.

    abstract:OBJECTIVES:Predictors of the postoperative quality of life (QoL) following ileal pouch anal anastomosis (IPAA) have not been thoroughly investigated. This study was planned to assess the postoperative QoL following IPAA and to identify its predictors using the 15D instrument. MATERIALS AND METHODS:A retrospective coho...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2017.1337218

    authors: Abolfotouh S,Rautio T,Klintrup K,Helavirta I,Mäkelä J

    更新日期:2017-10-01 00:00:00

  • Health care costs associated with Australian tertiary inflammatory bowel disease care.

    abstract:INTRODUCTION:We aimed to describe the total costs of illness for IBD patients and compare the costs of patients with active disease to those with inactive disease. MATERIALS AND METHODS:Resource use for IBD management was itemized for attributable costs (AUD) among all IBD patients over a 12-month period at an Austral...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2017.1323117

    authors: Jackson B,Con D,Ma R,Gorelik A,Liew D,De Cruz P

    更新日期:2017-08-01 00:00:00

  • Percutaneous transgallbladder rendezvous for enteroscopic management of choledocholithiasis in patients with surgically altered anatomy.

    abstract:INTRODUCTION:Endoscopic retrograde cholangiopancreatography (ERCP) in patients with surgically altered upper gastrointestinal anatomy (SAA) is generally challenging despite the use of enteroscopy. After failed biliary cannulation, rendezvous technique (RV) can be an option to assist the biliary access. However, proper ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2013.805812

    authors: Okuno M,Iwashita T,Yasuda I,Mabuchi M,Uemura S,Nakashima M,Doi S,Adachi S,Mukai T,Moriwaki H

    更新日期:2013-08-01 00:00:00

  • Crohn's disease of the distal large bowel.

    abstract::This study describes the clinical features and management of 80 patients with Crohn's disease affecting initially only the rectum with or without involvement of the sigmoid colon. Most patients were treated medically, and the various measures used are considered; the cumulative probability of completing five years und...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:

    authors: Ritchie JK,Lennard-Jones JE

    更新日期:1976-01-01 00:00:00

  • Do bile acids exert a negative feedback control of cholecystokinin release?

    abstract::The influence of intraduodenal bile deficiency due to chronic bile duct obstruction and acute exogenous administration of bile acids on plasma cholecystokinin (CCK) and pancreatic polypeptide (PP) was investigated. Fourteen patients with tumor-induced bile duct stenosis and five healthy volunteers were given a liquid ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528909093053

    authors: Koop I,Koop H,Gerhardt C,Schafmayer A,Arnold R

    更新日期:1989-04-01 00:00:00

  • High incidence of extrapancreatic carcinoma in chronic pancreatitis.

    abstract::In the prospective clinical long-term study of 246 patients with chronic pancreatitis, 26 patients (24 men) developed 27 histologically proved malignant tumors (11%). Four additional patients with neoplasia were excluded (papilloma, two; Bowen's disease of the tonsils, one; and seminoma, one, occurring 8 years before ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528009181490

    authors: Ammann RW,Knoblauch M,Möhr P,Deyhle P,Largiadèr F,Akovbiantz A,Schüler G,Schneider J

    更新日期:1980-01-01 00:00:00

  • Helicobacter pylori and different topographic types of gastritis: treatment response after successful eradication therapy in functional dyspepsia.

    abstract:BACKGROUND:Helicobacter pylori gastritis is usually a lifelong disease which can cause different topographical inflammatory reactions and induce divergent effects on acid secretion in humans. High acid output and antrum-predominant gastritis are associated with duodenal ulcer disease, whereas corpus-predominat gastriti...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Koskenpato J,Färkkilä M,Sipponen P

    更新日期:2002-07-01 00:00:00

  • Pre-endoscopic medication. A randomized double-blind trial of atropine and meperidine as a supplement to diazepam.

    abstract::The effects of an intramuscular injection of atropine (0.6 mg) and meperidine (1 mg/kg body weight) 30 min before topical benzocaine and intravenous diazepam administration were compared with those of a control group that received only benzocaine and intravenous diazepam in a randomized double-blind controlled trial o...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/00365527909181948

    authors: Schwartz SE,Fazio TL

    更新日期:1979-01-01 00:00:00

  • Helicobacter pylori infection inhibits antral mucosal nitric oxide production in humans.

    abstract:BACKGROUND:Inducible NO synthase expression is upregulated in H. pylori-infected gastric mucosa, suggesting increased NO synthesis as part of a host defense reaction. This study investigates actual NO production in the human antrum in situ. METHODS:Gastroscopy with antral biopsy sampling and intragastric tonometric NO...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655202317316024

    authors: von Bothmer C,Edebo A,Lönroth H,Olbe L,Pettersson A,Fändriks L

    更新日期:2002-04-01 00:00:00

  • A prospective epidemiologic study of Crohn's disease in Heraklion, Crete. Incidence over a 5-year period.

    abstract:BACKGROUND:There has been an impression from published work that Crohn's disease is less common in southern than in northern Europe. A low incidence of Crohn's disease has been observed in Greece, but conclusive data are still lacking. METHOD:A 5-year prospective and population-based epidemiologic study of Crohn's dis...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529609009134

    authors: Manousos ON,Koutroubakis I,Potamianos S,Roussomoustakaki M,Gourtsoyiannis N,Vlachonikolis IG

    更新日期:1996-06-01 00:00:00

  • The postcholecystectomy syndrome: bile ducts as pain trigger zone.

    abstract::Sixty-five non-icteric patients presumed to have the postcholecystectomy syndrome (PCS) were followed up for 4-13 years after their first endoscopic retrograde cholangiopancreatography (ERCP) examination, which gave normal findings. All patients, however, experienced severe pain on injection of only 1-2 ml of contrast...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528809093863

    authors: Lasson A,Fork FT,Trägårdh B,Zederfeldt B

    更新日期:1988-04-01 00:00:00