Temporal variations of adenosine metabolism in human blood.

Abstract:

:Eight diurnally active (06:00-23:00 h) subjects were adapted for 2 days to the room conditions where the experiments were performed. Blood sampling for adenosine metabolites and metabolizing enzymes was done hourly during the activity span and every 30 min during sleep. The results showed that adenosine and its catabolites (inosine, hypoxanthine, and uric acid), adenosine synthesizing (S-adenosylhomocysteine hydrolase and 5'-nucleotidase), degrading (adenosine deaminase) and nucleotide-forming (adenosine kinase) enzymes as well as adenine nucleotides (AMP, ADP, and ATP) undergo statistically significant fluctuations (ANOVA) during the 24 h. However, energy charge was invariable. Glucose and lactate chronograms were determined as metabolic indicators. The same data analyzed by the chi-square periodogram and Fourier series indicated ultradian oscillatory periods for all the metabolites and enzymatic activities determined, and 24-h oscillatory components for inosine, hypoxanthine, adenine nucleotides, glucose, and the activities of SAH-hydrolase, 5'-nucleotidase, and adenosine kinase. The single cosinor method showed significant oscillatory components exclusively for lactate. As a whole, these results suggest that adenosine metabolism may play a role as a biological oscillator coordinating and/or modulating the energy homeostasis and physiological status of erythrocytes in vivo and could be an important factor in the distribution of purine rings for the rest of the organism.

journal_name

Chronobiol Int

authors

Chagoya de Sánchez V,Hernández-Muñoz R,Suárez J,Vidrio S,Yáñez L,Aguilar-Roblero R,Oksenberg A,Vega-González A,Villalobos L,Rosenthal L,Fernández-Cancino F,Drucker-Colín R,Díaz-Muñoz M

doi

10.3109/07420529609012650

subject

Has Abstract

pub_date

1996-08-01 00:00:00

pages

163-77

issue

3

eissn

0742-0528

issn

1525-6073

journal_volume

13

pub_type

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