Abstract:
:Diseases of the nasopharyngeal epithelium due to Epstein-Barr virus (EBV) infection typically occur in chronic virus carriers with preexisting virus-specific antibodies. In vitro studies have shown that EBV-specific immunoglobulin A (IgA) promotes infection of human epithelial cells, otherwise refractory to EBV, via the polymeric immunoglobulin receptor (pIgR). To determine if EBV similarly exploits IgA transport mechanisms in vivo, we examined the fate of IgA-EBV complexes in the blood of mice, where pIgR-mediated transcytosis of IgA immune complexes through hepatocytes eliminates exogenous antigens from the circulation. By PCR analysis we showed hepatobiliary transport of IgA-EBV in viremic mice, but without detectable hepatocellular infection by immunostaining. Because efficient transport of EBV immune complexes might avert an infectious outcome, we modulated the transcytotic pathway in polarized Madin-Darby canine kidney (MDCK) cells transfected with pIgR to determine the effect on viral antigen expression. Like hepatocytes in vivo, MDCK cells in polarized monolayers translocated IgA-EBV from the basal cell face into apical medium without evidence for infection. However, when exposed to IgA-EBV as unpolarized single-cell suspensions, MDCK cells expressed EBV immediate-early and early antigens. These results suggest that pIgR-mediated transcytosis of pIgA-EBV through epithelium facilitates endogenous spread of EBV in long-term virus carriers, with infection being confined to cells with altered polarity from prior cytopathology.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gan YJ,Chodosh J,Morgan A,Sixbey JWdoi
10.1128/JVI.71.1.519-526.1997subject
Has Abstractpub_date
1997-01-01 00:00:00pages
519-26issue
1eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::At mucosal sites, papillomavirus genomes can persist in the epithelial basal layer following immune-mediated regression. Subsequent T-cell depletion stimulates a 3- to 5-log increase in the viral copy number, to levels associated with productive infection. Reappearance of microlesions was rare within the short time fr...
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doi:10.1128/JVI.19.2.709-716.1976
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doi:10.1128/JVI.27.2.313-319.1978
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pub_type: 杂志文章
doi:10.1128/JVI.70.7.4538-4548.1996
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.44.2.752-754.1982
更新日期:1982-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.173-184.1990
更新日期:1990-01-01 00:00:00
abstract::The highly syncytium-inducing M strain and the weakly syncytium-inducing SC strain of bovine parainfluenza 3 virus differ by a single amino acid substitution in each of the hemagglutinin-neuraminidase (HN) and membrane (M) proteins, while their fusion (F) proteins are identical (T. Shioda, S. Wakao, S. Suzu, and H. Sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3661-3668.1989
更新日期:1989-09-01 00:00:00
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doi:10.1128/JVI.22.3.794-803.1977
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7362-7366.1995
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pub_type: 杂志文章
doi:10.1128/JVI.25.1.60-72.1978
更新日期:1978-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.1.67-74.1986
更新日期:1986-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00