Abstract：
:The hypoxia-inducible factor-1 (HIF-1) was first described as a DNA binding activity that specifically recognizes an 8 bp hypoxia response element (HRE) known to be essential for oxygen-regulated erythropoietin gene expression. In electrophoretic mobility shift assays (EMSAs) HIF-1 DNA binding activity is only detectable in nuclear extracts of cells cultivated in a low oxygen atmosphere. In addition to HIF-1, a constitutive DNA binding activity also specifically binds the HIF-1 probe. Based on EMSAs using competitor oligonucleotides, specific antibodies and recombinant proteins, we previously reported that the constitutive HRE binding factor is composed of ATF-1 and CREB-1. Here we show that this site is functionally responsive to the cAMP agonist 8Br-cAMP in a dose-dependent manner under hypoxic but not under normoxic conditions. These results were confirmed by using the protein kinase A (PKA) activator Sp-cAMPS and the PKA inhibitor Rp-cAMPS: while Sp-cAMPS was synergistic with hypoxia on the HIF-1 DNA recognition site, the Rp-cAMPS isomer showed no effect. Our findings suggest that the PKA-signaling pathway is enhancing oxygen-dependent gene expression via the HRE.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Kvietikova I,Wenger RH,Marti HH,Gassmann Mdoi
10.1038/ki.1997.80subject
Has Abstractpub_date
1997-02-01 00:00:00pages
564-6issue
2eissn
0085-2538issn
1523-1755pii
S0085-2538(15)59920-1journal_volume
51pub_type
杂志文章abstract：:Studies were performed in anesthetized dogs (N = 7) to determine the effects of synthetic atrial natriuretic peptide (ANP) on sodium excretion in the presence and absence of control of glomerular filtration rate produced by suprarenal aortic clamping. Intrarenal infusion of synthetic atrial natriuretic peptide (0.3 mi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1986.144
更新日期：1986-07-01 00:00:00
abstract：:Tubulointerstitial nephritis antigen (TIN-ag) is an extracellular matrix (ECM) glycoprotein that has been recently isolated and cloned from the rabbit kidney. It is an integral component of the basal lamina, and unlike other basement membrane proteins it is exclusively expressed in the tubular basement membranes (TBMs...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.375
更新日期：1997-09-01 00:00:00
abstract：:Methods for estimating glomerular volume were compared in Zenker-fixed, paraffin-embedded biopsies from 10 patients with insulin-dependent diabetes mellitus and 6 normal kidney donors. Two methods of measurement of individual glomerular volumes were used: the Cavalieri method (considered the "gold standard") and the m...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.165
更新日期：1992-04-01 00:00:00
abstract：:Nephropathy is among the most frequent complications of diabetes and the leading cause of end-stage renal disease. Despite the success of novel drugs in animal models, the majority of the subsequent clinical trials employing those drugs targeting diabetic nephropathy failed. This lack of translational value may in par...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.06.023
更新日期：2016-11-01 00:00:00
abstract：:Podocyte foot process effacement is characteristic of proteinuric renal diseases. In minimal change nephrotic syndrome (MCNS) foot processes are diffusely effaced whereas the extent of effacement varies in focal segmental glomerulosclerosis (FSGS). Here we measured foot process effacement in FSGS and compared it to th...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.413
更新日期：2008-12-01 00:00:00
abstract：:The enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD) is thought to be a protective enzyme of the mineralocorticoid receptor (MR). We have previously demonstrated (Bonvalet et al, J Clin Invest 86:832-837, 1990) that 11 beta-OHSD is colocalized with MR along the rabbit nephron. In the present study, we examin...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.260
更新日期：1992-07-01 00:00:00
abstract：:With a lifetime incidence of up to 12% in man and 6% in woman, nephrolithiasis is a major health problem worldwide. Approximately, 80% of kidney stones are composed of calcium and hypercalciuria is found in up to 40% of stone-formers. Although the mechanisms resulting in precipitation and growth of calcium crystals in...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5002441
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND:A family history increases the risk of kidney stone passage independent of dietary risk factors. However, the metabolic basis for familial aggregation of urolithiasis is unknown. METHODS:We evaluated metabolic risk factors in families with at least two sibs with a history of calcium stones. Sibs underwent o...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.0600031141.x
更新日期：2001-09-01 00:00:00
abstract：:In landmark clinical trials, pharmacological inhibition of the renin-angiotensin system (RAS) with angiotensin-converting enzyme inhibitors (ACEIs) attenuated the decline in renal function associated with chronic renal disease (CRD). Hemodynamic and nonhemodynamic effects of angiotensin II (Ang II) attest to its centr...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1046/j.1523-1755.2000.00031.x
更新日期：2000-05-01 00:00:00
abstract：BACKGROUND:Redox-active iron can promote oxidative stress and tissue injury by catalyzing hydroxyl radical generation and lipid peroxidation. Intravenous iron preparations are routinely administered in conjunction with erythropoietin to treat anemia in patients with chronic renal failure (CRF), a condition that is mark...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2004.00580.x
更新日期：2004-05-01 00:00:00
abstract：:Adynamic bone disease (ABD) is increasingly recognized, especially in dialysis patients treated with oral calcium carbonate, vitamin D supplements, or supraphysiological dialysate calcium. We undertook this study to assess the effect of lowering dialysate calcium on episodes of hypercalcemia, serum parathyroid hormone...
journal_title：Kidney international
pub_type： 杂志文章,随机对照试验
doi：10.1038/sj.ki.5001666
更新日期：2006-09-01 00:00:00
abstract：BACKGROUND:Elimination of residual proteinuria is the novel target in renoprotection; nevertheless, whether a greater suppression of renin-angiotensin system (RAS) effectively improves the antiproteinuric response in patients with moderate proteinuria remains ill-defined. METHODS:We evaluated the effects of maximizing...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1046/j.1523-1755.2003.00015.x
更新日期：2003-06-01 00:00:00
abstract：:Adhesion of calcium oxalate (CaOx) crystals to kidney cells may be a key event in the pathogenesis of kidney stones associated with marked hyperoxaluria. Previously, we found that 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG), isolated from a traditional medicinal herb, reduced CaOx crystal adhesion to renal epithelial ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.458
更新日期：2011-03-01 00:00:00
abstract：:We examined the potential of in vivo linkage of plasminogen activator inhibitor-1 (PAI-1) and angiotensin II (Ang II) in the setting of endothelial injury and sclerosis following radiation injury in the rat. PAI-1 is a major physiological inhibitor of the plasminogen activator (PA)/plasmin system, a key regulator of f...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.20
更新日期：1997-01-01 00:00:00
abstract：:Pathogenesis of vascular calcification in chronic kidney disease. Background. Hyperphosphatemia and hypercalcemia are independent risk factors for higher incidence of cardiovascular events in patients with chronic kidney disease. In addition to increased calcium-phosphate product, hyperphosphatemia accelerates the pro...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1111/j.1523-1755.2005.00421.x
更新日期：2005-08-01 00:00:00
abstract：:Many subjects nowadays present with end-stage renal failure and its attendant cardiovascular complications without known prior renal damage. In this report we review the evidence available to strongly suggest that the present practice of secondary prevention in those with known prior renal disease should be extended t...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1111/j.1523-1755.2004.66001.x
更新日期：2004-12-01 00:00:00
abstract：:The hormonal responsiveness profile of the cortical collecting duct varies from one species to another. To identify the hormones and agonists that modulate the functions of this tubule segment in the human species, we generated a cell line (HCD) immortalized by SV40 virus. The tubular origin of this cell line was asse...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.186
更新日期：1995-05-01 00:00:00
abstract：:Adoptive immunotherapy in patients with advanced cancer produces significant regression of metastatic disease in selected patients, but it is complicated by severe side effects. Prevention of these complications is still limited because their precise mechanisms remain unknown. For this reason we have investigated rena...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1991.215
更新日期：1991-08-01 00:00:00
abstract：:Chemotherapy-induced nephrotoxicity limits the success of cancer therapy. Landau et al. now describe a mechanism by which a first dose of cisplatin renders the kidney sensitive to necroptosis mediated by a second dose. Unresolved injury and sustained necrosis, therefore, may represent a pathophysiological means of tra...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2018.12.004
更新日期：2019-04-01 00:00:00
abstract：:Intrarenal artery infusions of hypertonic saline can activate tubuloglomerular feedback (TGF), decreasing renal blood flow (RBF) and glomerular filtration rate (GFR). The response to infusion of hypertonic saline is enhanced by salt depletion and attenuated by salt loading, but has not previously been investigated in ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1987.236
更新日期：1987-10-01 00:00:00
abstract：:Hemodialysis adequacy can be quantified using ultraviolet absorbance of the spent dialysate, or by analysis of dialysate conductivity at the dialyzer inlet and outlet in response to changes in dialysate electrolyte concentration. These measurements can be made at every dialysis, including initial and acute treatments ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2010.218
更新日期：2010-11-01 00:00:00
abstract：:How much dialysis is required for optimal well-being and long-term survival? The Frequent Hemodialysis Network completed two prospective trials examining the effect of more frequent, short hours and, now, of nocturnal hemodialysis compared with standard thrice-weekly treatments. Whereas the short-hours trial reported ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2011.267
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND:An imbalance between protein load and folding capacity is referred to as endoplasmic reticulum (ER) stress. As a defense mechanism, cells express ER stress inducible chaperons, such as oxygen-regulated proteins 150 (ORP150) and glucose-regulated proteins (GRPs). While ER stress is important in various diseas...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00736.x
更新日期：2005-12-01 00:00:00
abstract：:The risk of developing contrast media-induced acute renal failure was studied in 49 randomly selected nonazotemic type 2 adult diabetic patients subjected to IVU. There were 19 men and 30 women in the group whose mean age was 62 +/- 10 years (range, 38 to 82 years). In preparation for IVU, patients were neither dehydr...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1982.91
更新日期：1982-05-01 00:00:00
abstract：:Iron is critical for cellular, organismal, and possibly universal existence. Use of iron complexes to treat human diseases is ancient and is described in detail in Ayurveda/Siddha systems of medicine. Old aphorisms from Siddha medicine ("Alavukku Minjinal Amirdhamum Nanjagum," an elixir turns poisonous when taken in e...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2016.02.035
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:This multicenter, open-label, dose-titration study assessed the safety and efficacy of RenaGel(R), a nonabsorbed calcium- and aluminum-free phosphate binder, in lowering serum phosphorus. Secondary outcomes were its effects on serum intact parathyroid hormone (iPTH) and serum lipids. METHODS:Phosphate binde...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1046/j.1523-1755.1999.00240.x
更新日期：1999-01-01 00:00:00
abstract：:A major contributor to the development and progression of ischemia-reperfusion (IR)-induced acute renal failure (ARF) is the loss of functioning tubular epithelial cells by means of various cell deletion or death processes. Although the term "acute tubular necrosis" is still used to describe the pathology of ARF, this...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00701.x
更新日期：1999-10-01 00:00:00
abstract：:The microvillus brush border on the renal proximal tubule epithelium allows the controlled reabsorption of solutes that are filtered through the glomerulus and thus participates in general body homeostasis. Here, using the lipid 5-phosphatase Ship2 global knockout mice, proximal tubule-specific Ship2 knockout mice, an...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2017.01.008
更新日期：2017-07-01 00:00:00
abstract：BACKGROUND:The "intact" parathyroid hormone (PTH) assay recognizes PTH-(1-84) as well as amino terminally truncated PTH fragments, that is, large carboxyterminal PTH fragments (C-PTH fragments). The present study investigated whether the use of the plasma PTH-(1-84)/C-PTH fragment ratio enhances the noninvasive assessm...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00949.x
更新日期：2001-10-01 00:00:00
abstract：:Early identification of kidney transplant recipients at risk of progressive allograft dysfunction may allow clinicians to provide closer monitoring and more aggressive risk factor modification. In this issue, Raynaud et al. presented a latent class model that clustered kidney transplant recipients into 8 risk categori...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2020.07.031
更新日期：2021-01-01 00:00:00
