Abstract：
AIMS:Caveolae are membrane microdomains where important signalling pathways are assembled and molecular effects transduced. In this study, we hypothesized that shear stress-mediated vasodilation (SSD) of mouse small coronary arteries (MCA) is caveolae-dependent. METHODS AND RESULTS:MCA (80-150 μm) isolated from wild-type (WT) and caveolin-1 null (Cav-1(-/-)) mice were subjected to physiological levels of shear stress (1-25 dynes/cm(2)) with and without pre-incubation of inhibitors of nitric oxide synthase (L-NAME), cyclooxygenase (indomethacin, INDO), or cytochrome P450 epoxygenase (SKF 525A). SSD was endothelium-dependent in WT and Cav-1(-/-) coronaries but that in Cav-1(-/-) was significantly diminished compared with WT. Pre-incubation with L-NAME, INDO, or SKF 525A significantly reduced SSD in WT but not in Cav-1(-/-) mice. Vessels from the soluble epoxide hydrolase null (Ephx2(-/-)) mice showed enhanced SSD, which was further augmented by the presence of arachidonic acid. In donor-detector-coupled vessel experiments, Cav-1(-/-) donor vessels produced diminished dilation in WT endothelium-denuded detector vessels compared with WT donor vessels. Shear stress elicited a robust intracellular Ca(2+) increase in vascular endothelial cells isolated from WT but not those from Cav-1(-/-) mice. CONCLUSION:Integrity of caveolae is critical for endothelium-dependent SSD in MCA. Cav-1(-/-) endothelium is deficient in shear stress-mediated generation of vasodilators including NO, prostaglandins, and epoxyeicosatrienoic acids. Caveolae plays a critical role in endothelial signal transduction from shear stress to vasodilator production and release.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Chai Q,Wang XL,Zeldin DC,Lee HCdoi
10.1093/cvr/cvt157subject
Has Abstractpub_date
2013-10-01 00:00:00pages
151-9issue
1eissn
0008-6363issn
1755-3245pii
cvt157journal_volume
100pub_type
杂志文章abstract：AIMS:Sphingosine-1-phosphate (S1P), a key regulator of vascular homeostasis and angiogenesis, promotes endothelial cell migration via stimulation of phosphoinositide 3-kinase (PI3K). The aim of this study was to identify the role of PI3Kbeta and gamma isoforms and their downstream effector pathways in mediating endothe...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvn159
更新日期：2008-10-01 00:00:00
abstract：AIMS:Doxorubicin (DOX) is a widely used and effective anti-cancer therapeutic. DOX treatment is associated with both acute and late onset cardiotoxicity, limiting its overall efficacy. Here, the impact of cardiomyocyte cell cycle activation was examined in a juvenile model featuring aspects of acute and late onset DOX ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvy273
更新日期：2019-04-15 00:00:00
abstract：AIMS:Mobilization of stem cells/progenitors is regulated by the interaction between stromal cell-derived factor-1 (SDF-1) and its ligand, CXC chemokine receptor 4 (CXCR4). Statins have been suggested to ameliorate pulmonary arterial hypertension (PAH); however, the mechanisms involved, especially their effects on proge...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvn244
更新日期：2009-01-01 00:00:00
abstract：AIMS:Atrial fibrillation (AF) is the most common type of arrhythmia in humans, yet the genetic cause of AF remains elusive. Genome-wide association studies (GWASs) have reported risk variants in four distinct genetic loci, and more recently, a meta-GWAS has further implicated six new loci in AF. However, the functional...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvv207
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVE:Diastolic dysfunction is a characteristic finding of the aged mammalian heart. Parvalbumin acts as a Ca2+ sink and enhances relaxation in skeletal muscle, and overexpression of parvalbumin in myocardium increased cardiac relaxation in vitro as well as in vivo. Therefore, the objective of this study is to test...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2004.06.028
更新日期：2005-05-01 00:00:00
abstract：:The rate of QT adaptation to abrupt changes in pacing rate was studied in seven patients with newly diagnosed complete heart block with a ventricular escape rate of less than 40 beats.min-1. Their median age was 70 (range 36-84) years, and none was taking any cardioactive medication known to affect the QT interval. Fr...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/22.1.67
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVE:Hereditary long QT syndrome (LQTS) is a genetically heterogeneous disease characterized by prolonged QT intervals and an increased risk for ventricular arrhythmias and sudden cardiac death. Mutations in the voltage-gated potassium channel subunit KCNQ1 induce the most common form of LQTS. KCNQ1 is associated ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2005.05.003
更新日期：2005-08-15 00:00:00
abstract：:The development of the heart is a complex process during which different cell types progressively contribute to shape a four-chambered pumping organ. Over the last decades, our understanding of the specification and transcriptional regulation of cardiac development has been greatly augmented as has our understanding o...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/cvr078
更新日期：2011-07-15 00:00:00
abstract：OBJECTIVE:Growth hormone (GH) is known to induce growth of the normal rat heart. Whether this growth is due solely to hypertrophy of the cardiac myocytes or whether a concomitant hyperplasia of the cardiac myocytes also takes place is currently not known. Therefore, the aim of the present study was to investigate wheth...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2007.06.026
更新日期：2007-12-01 00:00:00
abstract：AIMS:A problem in generating artificial tissues is supplying nutrients to cells within 3D constructs. The use of a decellularized biological vascularized matrix with preserved pedicles (BioVaM), as a scaffold, appears to aid the generation of perfusable tissue constructs in vitro. To prevent vessel occlusion upon impla...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvp370
更新日期：2010-03-01 00:00:00
abstract：:To improve assessment of ventricular function during cardiac catheterisation there should be available a continuous registration of stroke volume and cardiac output in addition to ventricular pressure. To obtain the desired volumetric quantities a catheter has been developed which measures changes in intraventricular ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/15.6.328
更新日期：1981-06-01 00:00:00
abstract：OBJECTIVE:Effective endogenous fibrinolysis requires rapid release of endothelial tissue plasminogen activator (t-PA). Using the nitric oxide synthase inhibitor, L-NG-monomethylarginine (L-NMMA), we examined the contribution of endogenous nitric oxide to substance P-induced t-PA release in vivo in man. METHODS:Blood f...
journal_title：Cardiovascular research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0008-6363(98)00017-0
更新日期：1998-05-01 00:00:00
abstract：:Circulating concentrations of leucocyte elastase and free radical activity were measured in 11 adults undergoing cardiopulmonary bypass. In all patients the bypass procedure was associated with pronounced changes in plasma elastase concentrations, and peak enzyme concentrations correlated closely with the duration of ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/22.1.37
更新日期：1988-01-01 00:00:00
abstract：:The therapeutic potential of Hedgehog (Hh) signalling agonists for vascular diseases is of growing interest. However, molecular and cellular mechanisms underlying the role of the Hh signalling in vascular biology remain poorly understood. AIMS:The purpose of the present paper is to clarify some conflicting literature...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvaa285
更新日期：2020-10-16 00:00:00
abstract：AIMS:For the majority of congenital heart diseases (CHDs), the full complexity of the causative molecular network, which is driven by genetic, epigenetic, and environmental factors, is yet to be elucidated. Epigenetic alterations are suggested to play a pivotal role in modulating the phenotypic expression of CHDs and t...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvw195
更新日期：2016-10-01 00:00:00
abstract：:Atherosclerosis is considered to be a form of chronic inflammation governed by a complex network of inter- and intra-cellular signaling pathways. The pleiotropic cytokine interferon-gamma (IFN-gamma) is a key pro-inflammatory mediator that is expressed at high levels in atherosclerotic lesions. IFN-gamma regulates the...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2005.04.019
更新日期：2005-07-01 00:00:00
abstract：:Most studies on stem cell transplantation therapy on myocardially infarcted animal models and phase-I human clinical trials have focused on the use of undifferentiated stem cells. There is a strong possibility that some degree of cardiomyogenic differentiation of stem cells in vitro prior to transplantation would resu...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2003.12.022
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVE:The role of phosphorylation of the ryanodine receptor at serine-2808 (RyRS2808) in congestive heart failure (CHF) is controversial, and effects of RyRS2808 phosphorylation on contraction are unclear. It has been reported that diastolic sarcomere length (SL) fluctuations accompany propagating contractile waves...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2005.07.010
更新日期：2006-01-01 00:00:00
abstract：OBJECTIVES:To characterize the 'anti-ischemic' effects of adenosine metabolism inhibition in ischemic-reperfused myocardium. METHODS:Perfused C57/B16 mouse hearts were subjected to 20 min ischemia 40 min reperfusion in the absence or presence of adenosine deaminase inhibition (50 microM erythro-2-(2-hydroxy-3-nonyl)ad...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(01)00360-1
更新日期：2001-10-01 00:00:00
abstract：:Protein kinase A (PKA) is a central regulator of cardiac performance and morphology. Myocardial PKA activation is induced by a variety of hormones, neurotransmitters and stress signals, most notably catecholamines secreted by the sympathetic nervous system. Catecholamines bind β-adrenergic receptors to stimulate cAMP-...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvab008
更新日期：2021-01-23 00:00:00
abstract：AIM:The aim of the study was to determine the potential for K(V)1 potassium channel blockers as inhibitors of human neoinitimal hyperplasia. METHODS AND RESULTS:Blood vessels were obtained from patients or mice and studied in culture. Reverse transcriptase-polymerase chain reaction and immunocytochemistry were used to...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvq305
更新日期：2011-02-01 00:00:00
abstract：:In isolated electrically stimulated guinea pig papillary muscles tiapamil exerts a negative inotropic action that corresponds to its presumptive inhibitory effects on the slow-channel-mediated transmembrane Ca2+ influx. However, contractile depression is always accompanied by marked alterations of the normal Na+-carri...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/20.1.42
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND:Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligands have been shown to ameliorate a variety of inflammatory conditions. The present study tested the hypothesis that PPAR-gamma ligands reduce experimental autoimmune myocarditis (EAM) associated with inhibition of the expansion and activation...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(03)00457-7
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:The aims were: (1) to evaluate whether differences in absolute and cyclic echocardiographic image amplitude exist in different layers (subendocardium and subepicardium) and regions (septal, anterior, lateral, inferior wall) of the canine left ventricle; (2) to assess the dependence of these variables upon loc...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/27.2.279
更新日期：1993-02-01 00:00:00
abstract：OBJECTIVE:Myocardial adaptation has been reported to result from mild but chronic ischaemia in the hearts of patients with coronary artery disease. The aim of this study was to test the hypothesis that the responses of the chronically hypoxic myocardium to an episode of severe ischaemia, or the effects of acute ischaem...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/28.3.312
更新日期：1994-03-01 00:00:00
abstract：AIMS:n-3 Polyunsaturated fatty acids (PUFAs) are known to protect the cardiovascular system and improve blood pressure control. These important dietary constituents are converted into bioactive metabolites, but their role in regulation of the cardiovascular system is unclear. In particular, the functions of the cytochr...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvq411
更新日期：2011-05-01 00:00:00
abstract：:18 patients without valvular pathology, coronary artery disease, or idiopathic hypertrophic subaortic stenosis were haemodynamically and angiographically investigated in order to analyse the effects of a ventricular extrasystolic beat upon the post-extrasystolic left ventricular peak pressure. In eight normal patients...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/13.6.338
更新日期：1979-06-01 00:00:00
abstract：:Diabetes is one of the most prevalent chronic conditions that has a high association with death from cardiovascular disease(s). An impaired cardiac function independent of vascular disease suggests the existence of a primary myocardial defect in diabetes mellitus. We and others have documented that myocardial performa...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/s0008-6363(97)00059-x
更新日期：1997-04-01 00:00:00
abstract：OBJECTIVE:Previous studies showed that treatment of hearts with nisoldipine improves recovery of cardiac function following ischaemia, improves reperfusion, and reduces the constrictor sensitivity to endothelin. The aim of the present study was to assess the reduction in vasodilator responses that occurs following isch...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/27.6.990
更新日期：1993-06-01 00:00:00
abstract：Aims:Hypertensive cardiac hypertrophy is associated with reduced coronary flow reserve, but its impact on coronary flow regulation and vasomotor function remains incompletely understood and requires further investigation. Methods and results:Left ventricular hypertrophy was induced in mice by transverse aortic coarcta...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvx103
更新日期：2017-09-01 00:00:00
