Abstract：
OBJECTIVES:To characterize the 'anti-ischemic' effects of adenosine metabolism inhibition in ischemic-reperfused myocardium. METHODS:Perfused C57/B16 mouse hearts were subjected to 20 min ischemia 40 min reperfusion in the absence or presence of adenosine deaminase inhibition (50 microM erythro-2-(2-hydroxy-3-nonyl)adenine; EHNA) adenosine kinase inhibition (10 microM iodotubercidin; IODO), or 10 microM adenosine. Hearts overexpressing A(1) adenosine receptors (A(1)ARs) were also studied. RESULTS:EHNA treatment reduced ischemic contracture and post-ischemic diastolic pressure (14+/-2 vs. 20+/-1 mmHg), increased recovery of developed pressure (66+/-3 vs. 53+/-2%) and reduced LDH efflux (8.9+/-1.6 vs. 18.0+/-1.7 I.U./g). IODO also improved functional recovery (to 60+/-2%) and reduced LDH efflux (5.3+/-1.7 I.U./g), as did treatment with 10 microM adenosine. Protection with EHNA was reversed by co-infusion of IODO or 50 microM 8-rho-sulfophenyltheophylline (adenosine receptor antagonist), but unaltered by 20 microM inosine+10 microm hypoxanthine. Similarly, effects of iodotubercidin were inhibited by EHNA and 8-rho-sulfophenyltheophylline. A(1)AR overexpression exerted similar effects to EHNA and EHNA or IODO alone enhanced recovery while EHNA+IODO reduced recovery in transgenic hearts. Functional recoveries and xanthine oxidase reactant levels were unrelated in the groups studied. CONCLUSIONS:Adenosine deaminase or kinase inhibition protects from ischemia-reperfusion. Cardioprotection via these enzyme inhibitors requires a functioning purine salvage pathway and involves enhanced adenosine receptor activation. Reduced formation of inosine is unimportant in EHNA-mediated protection.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Peart J,Matherne GP,Cerniway RJ,Headrick JPdoi
10.1016/s0008-6363(01)00360-1subject
Has Abstractpub_date
2001-10-01 00:00:00pages
120-9issue
1eissn
0008-6363issn
1755-3245pii
S0008636301003601journal_volume
52pub_type
杂志文章abstract：AIMS:Our objective was to explore the functional interdependence of protein kinase A (PKA) phosphorylation with binding of modulatory FK506 binding proteins (FKBP12/12.6) to the ryanodine receptor (RyR). RyR type 1 or type 2 was prepared from rabbit skeletal muscle or pig cardiac muscle, respectively. In heart failure,...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvp273
更新日期：2010-01-01 00:00:00
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journal_title：Cardiovascular research
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doi：10.1093/cvr/cvz145
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abstract：:The signal transducer and activator of transcription 3 (STAT3) is fundamental for physiological homeostasis and stress-induced remodelling of the heart as deregulated STAT3 circuits are sufficient to induce dilated and peripartum cardiomyopathy and adverse remodelling after myocardial infarction. STAT3 activity depend...
journal_title：Cardiovascular research
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更新日期：2014-05-01 00:00:00
abstract：OBJECTIVE:Our aim was to determine in desmin homozygous mutant mice the viscoelastic properties, the mechanical strength and the structure of the carotid artery. METHODS:To assess the viscoelastic properties of large arteries, we have performed an in vivo analysis of the diameter-, and distensibility-pressure curves o...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(01)00278-4
更新日期：2001-07-01 00:00:00
abstract：AIMS:Moderate wine consumption is associated with a significant reduction of cardiovascular mortality. The molecular basis of this phenomenon remains unknown. Platelet-derived growth factor (PDGF) is an important contributor to atherogenesis. We investigated the effects of selected red and white wines on PDGF receptor ...
journal_title：Cardiovascular research
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doi：10.1093/cvr/cvn340
更新日期：2009-03-01 00:00:00
abstract：:Vasculopathy is a pathological process occurring in the blood vessel wall, which could affect the haemostasis and physiological functions of all the vital tissues/organs and is one of the main underlying causes for a variety of human diseases including cardiovascular diseases. Current pharmacological interventions aim...
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更新日期：2020-11-02 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/s0008-6363(98)00110-2
更新日期：1998-07-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2005.10.004
更新日期：2006-02-15 00:00:00
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journal_title：Cardiovascular research
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doi：10.1093/cvr/cvt013
更新日期：2013-04-01 00:00:00
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journal_title：Cardiovascular research
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doi：10.1093/cvr/cvu029
更新日期：2014-04-01 00:00:00
abstract：OBJECTIVE:Inflammatory cells invade the fibrotic myocardium of spontaneously hypertensive rats at the same sites as where fibroblasts are produced. The role of these inflammatory cells in myocardial fibrogenesis was studied in the present work. METHODS:The production and distribution of proteins that may be implicated...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：OBJECTIVE:To investigate the possible mechanisms of the antiatherosclerotic effects of the angiotensin-converting enzyme (ACE) inhibitor, fosinopril, in apolipoprotein (apo) E deficient mice. METHODS:Apo E deficient (E0) mice at the age of 8 weeks received either placebo or a high dose (25 mg/kg/d) of fosinopril suppl...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(99)00239-4
更新日期：1999-12-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvaa070
更新日期：2021-01-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/21.9.660
更新日期：1987-09-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2004.06.028
更新日期：2005-05-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(01)00531-4
更新日期：2002-03-01 00:00:00
abstract：OBJECTIVE:The aim was to test for the presence of cAMPi activated and ATPo activated chloride conductances (ICl(cAMP) and ICl(ATP)) in mouse ventricular myocytes. METHODS:Membrane currents were measured in enzymatically dissociated ventricular myocytes using the whole cell configuration of the patch clamp technique. ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：OBJECTIVE:It has been proposed that human immunodeficiency virus (HIV) infection-induced inflammatory environment may contribute to the pathogenesis of cardiovascular diseases. Recent studies have also demonstrated the potential role of estrogen as therapeutic agents in the prevention or treatment of cardiovascular dis...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2004.03.006
更新日期：2004-07-01 00:00:00
abstract：:In order to evaluate clinically recorded jugular vein pulses it is necessary to understand the transmission process of the right atrial pressure pulse through the caval veins up to the jugular veins. The transmission speed at distinct points of the venous pressure curve was studied in the superior vena cava of 20 anae...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/17.10.627
更新日期：1983-10-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvy310
更新日期：2019-08-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2010-07-15 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2003.11.024
更新日期：2004-02-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvw250
更新日期：2017-03-01 00:00:00
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journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2007.05.005
更新日期：2007-09-01 00:00:00
abstract：:The most critical determinant of prognosis in patients with acute myocardial infarction (MI) is infarct magnitude, which can be established within several hours of an attack. The importance of the subsequent healing process is not negligible, however. In fact, much experimental and clinical evidence suggests that late...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/cvp032
更新日期：2009-07-15 00:00:00
abstract：:In this study we report about the modulation of connexin45 (Cx45) gap junction channel properties by phosphorylation of the connexin molecules through different protein kinases. Phosphorylation of Cx45 was studied in HeLa cells transfected with mouse Cx45 (mCx45). Using Western blotting (WB) and immunocytochemistry, t...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(00)00047-x
更新日期：2000-06-01 00:00:00
abstract：OBJECTIVE:Vascular endothelial growth factor (VEGF), secreted by vascular cells and a variety of tumour cells, is a potent angiogenic factor. Since nitric oxide (NO) seems to play a key role in the VEGF-induced proliferation of endothelial cells, the aim of the present study was to determine whether VEGF stimulates end...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(98)00228-4
更新日期：1999-03-01 00:00:00
abstract：:Extracellular potassium activity before and after coronary occlusion was measured in the canine heart by means of potassium-selective surface electrodes. In the ischaemic myocardium potassium activity rapidly increased from a preocclusion value of 3.2 +/- 0.3 mmol.litre-1 to 10 +/- 0.6 mmol.litre-1 within the first 5 ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/13.5.297
更新日期：1979-05-01 00:00:00
abstract：OBJECTIVES:I(Ks), the slow component of the delayed rectifier potassium current, underlies a strong beta-adrenergic regulation in the heart. Catecholamines, like isoproterenol, induce a strong increase in I(Ks). Recent work has pointed to an opposing biological effect of beta(1)- and beta(3)-adrenoceptors in the heart....
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(02)00601-6
更新日期：2002-12-01 00:00:00
abstract：AIMS:Regional heterogeneities in contraction contribute to heart failure with reduced ejection fraction (HFrEF). We aimed to determine whether regional changes in myocardial relaxation similarly contribute to diastolic dysfunction in post-infarction HFrEF, and to elucidate the underlying mechanisms. METHODS AND RESULT...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvy257
更新日期：2019-03-15 00:00:00
