当前位置： SCI文献检索 > KIDNEY INTERNATIONAL期刊下所有文献 > A method for accurate measurement of GFR in conscious, spontaneously voiding rats.

A method for accurate measurement of GFR in conscious, spontaneously voiding rats.

Abstract：

:Renal function measurement by clearance methods relies on accurately timed urine collection. In small experimental animals, renal function measurement is usually performed under anesthesia and/or with the application of bladder catheters to ensure accurate urine collection. To avoid both anesthesia and the need for bladder catheters we developed a method to measure glomerular filtration rate (GFR) in spontaneously voiding conscious rats. GFR was measured as the urinary clearance of constantly infused 125I-iothalamate. To correct for incomplete bladder emptying urinary clearance of 125I-iothalamate was multiplied by the ratio of plasma and urinary clearance of simultaneously infused 131I-hippuran, a correction method that has been previously validated in humans. Reproducibility of the technique was evaluated by analysis of the results of four consecutive clearance periods during the day (intra-assay variation) in a group of 17 rats and of two consecutive clearance periods on two or three separate days in a group of 20 rats (inter-assay variation), all with normal renal function. Application of the correction method reduced the intra-assay coefficient of variation (mean +/- SD) from 37.4 +/- 14.3 to 5.4 +/- 2.3% (P < 0.05). The mean inter-assay coefficient of variation fell slightly from 23.4 +/- 10.3 to 11.0 +/- 7.2% (P < 0.10). In rats with moderately impaired renal function (N = 8) the intra-assay variation fell from 27.9 +/- 20.7 to 2.7 +/- 1.6% (P < 0.05). Our data show that this correction method is a useful technique to assess renal function in conscious, spontaneously voiding rats.

journal_name

Kidney Int

journal_title

Kidney international

authors

Marcel de Vries PA,Navis G,de Boer E,de Jong PE,de Zeeuw D

doi

10.1038/ki.1997.327

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

244-7

issue

1

eissn

0085-2538

issn

1523-1755

pii

S0085-2538(15)60167-3

journal_volume

52

pub_type

杂志文章

相关文献

KIDNEY INTERNATIONAL文献大全
  • Cardiorenal effects of celecoxib as compared with the nonsteroidal anti-inflammatory drugs diclofenac and ibuprofen.

    abstract：:The cardiorenal safety database from the Celecoxib Long-term Arthritis Safety Study (CLASS) was analyzed to examine whether supratherapeutic doses of celecoxib are associated with decreased renal function and blood pressure (BP) effects compared with standard doses of diclofenac and ibuprofen in osteoarthritis (OA) an...

    journal_title：Kidney international

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/sj.ki.5001766

    authors： Whelton A,Lefkowith JL,West CR,Verburg KM

    更新日期：2006-10-01 00:00:00

  • Effects of hyperosmolality on ANP-stimulated cGMP generation in rat inner medullary collecting duct.

    abstract：:The inner medullary collecting duct (IMCD) is a major target site of atrial natriuretic peptide (ANP) for diuresis and natriuresis, and it is in a hypertonic condition made by the renal countercurrent multiplication system. We investigated the effects of hyperosmolality on ANP-stimulated cGMP generation in IMCD and gl...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1994.178

    authors： Shinohara M,Nonoguchi H,Ujiie K,Terada Y,Owada A,Tomita K,Marumo F

    更新日期：1994-05-01 00:00:00

  • Molecular interactions of FGF23 and PTH in phosphate regulation.

    abstract：:Bone-derived fibroblast growth factor-23 (FGF23) plays an important role in systemic phosphate turnover. Increased FGF23 activity results in hypophosphatemic disorders, while reduced activity is linked to hyperphosphatemic disorders. FGF23, together with klotho as co-factor, can activate FGF receptors in its target ti...

    journal_title：Kidney international

    pub_type： 评论,杂志文章

    doi：10.1038/ki.2014.316

    authors： Lanske B,Razzaque MS

    更新日期：2014-12-01 00:00:00

  • Klotho expression in osteocytes regulates bone metabolism and controls bone formation.

    abstract：:Osteocytes within the mineralized bone matrix control bone remodeling by regulating osteoblast and osteoclast activity. Osteocytes express the aging suppressor Klotho, but the functional role of this protein in skeletal homeostasis is unknown. Here we identify Klotho expression in osteocytes as a potent regulator of b...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1016/j.kint.2017.02.014

    authors： Komaba H,Kaludjerovic J,Hu DZ,Nagano K,Amano K,Ide N,Sato T,Densmore MJ,Hanai JI,Olauson H,Bellido T,Larsson TE,Baron R,Lanske B

    更新日期：2017-09-01 00:00:00

  • Renal handling of urea in subjects with persistent azotemia and normal renal function.

    abstract：:Fourteen subjects with persistent azotemia and normal glomerular filtration rate were studied by renal clearances and hormonal determinations to establish the nephron site of altered urea transport and the mechanism(s) responsible for their azotemia. During constant alimentary protein, urea nitrogen appearance was nor...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1987.266

    authors： Conte G,Dal Canton A,Terribile M,Cianciaruso B,Di Minno G,Pannain M,Russo D,Andreucci VE

    更新日期：1987-11-01 00:00:00

  • Reducing hospital readmissions in patients with end-stage kidney disease.

    abstract：:ESKD patients have a large burden of disease, with high rates of readmission to hospital compared with the general population. A readmission after an acute index hospital discharge is either planned or unplanned. A proportion of unplanned readmissions are potentially avoidable, and could have been prevented with optim...

    journal_title：Kidney international

    pub_type： 杂志文章,评审

    doi：10.1038/ki.2015.307

    authors： Mathew AT,Strippoli GF,Ruospo M,Fishbane S

    更新日期：2015-12-01 00:00:00

  • New mouse model for polycystic kidney disease with both recessive and dominant gene effects.

    abstract：:In the course of studying the genetics of chlorambucil mutagenesis, we have uncovered a new model for autosomal polycystic kidney disease (PKD). In the homozygous condition, the gene, jcpk, causes a very severe disease characterized by cysts in all segments of the nephron. Death usually occurs before 10 days of age. E...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1995.69

    authors： Flaherty L,Bryda EC,Collins D,Rudofsky U,Montogomery JC

    更新日期：1995-02-01 00:00:00

  • Acute renal failure independently predicts mortality after myeloablative allogeneic hematopoietic cell transplant.

    abstract：BACKGROUND:Patients undergoing myeloablative allogeneic hematopoietic cell transplantation (HCT) have a high incidence of acute renal failure (ARF). However, it is unclear if ARF is independently associated with mortality after this procedure. METHODS:We performed meta-analysis of published reports on ARF after myeloa...

    journal_title：Kidney international

    pub_type： 杂志文章,meta分析

    doi：10.1111/j.1523-1755.2005.00301.x

    authors： Parikh CR,McSweeney P,Schrier RW

    更新日期：2005-05-01 00:00:00

  • A network thermodynamic model of glomerular dynamics: application in the rat.

    abstract：:A model of glomerular dynamics has been developed by using network thermodynamics and the SPICE 2 computer program to further explore the determinants of glomerular filtration. The model is designed to be holistic and self-adjusting, taking cognizance of and permitting quantitation of the secondary alterations in indi...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1981.27

    authors： Oken DE,Thomas SR,Mikulecky DC

    更新日期：1981-02-01 00:00:00

  • Combination therapy with ACE inhibitors and angiotensin II receptor blockers to halt progression of chronic renal disease: pathophysiology and indications.

    abstract：:It is no a secret that we are confronted by an alarmingly increasing number of patients with progressive renal disease. There is ample evidence for the notion that angiotensin II (Ang II) is a major culprit in progression. The vasopeptide Ang II turned out to have also multiple nonhemodynamic pathophysiologic actions ...

    journal_title：Kidney international

    pub_type： 杂志文章,评审

    doi：10.1111/j.1523-1755.2005.00145.x

    authors： Wolf G,Ritz E

    更新日期：2005-03-01 00:00:00

  • Risk factors for cyclosporine-induced tubulointerstitial lesions in children with minimal change nephrotic syndrome.

    abstract：BACKGROUND:Cyclosporine (CsA) is effective for the treatment of children with steroid-dependent and -resistant nephrotic syndrome (NS), but it can result in chronic CsA nephrotoxicity including CsA-induced tubulointerstitial lesions. The factors responsible for the development of CsA-induced tubulointerstitial lesions ...

    journal_title：Kidney international

    pub_type： 临床试验,杂志文章

    doi：10.1046/j.1523-1755.2002.00303.x

    authors： Iijima K,Hamahira K,Tanaka R,Kobayashi A,Nozu K,Nakamura H,Yoshikawa N

    更新日期：2002-05-01 00:00:00

  • Cardiac dysfunction in Pkd1-deficient mice with phenotype rescue by galectin-3 knockout.

    abstract：:Alterations in myocardial wall texture stand out among ADPKD cardiovascular manifestations in hypertensive and normotensive patients. To elucidate their pathogenesis, we analyzed the cardiac phenotype in Pkd1(cond/cond)Nestin(cre) (CYG+) cystic mice exposed to increased blood pressure, at 5 to 6 and 20 to 24 weeks of ...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1016/j.kint.2016.04.028

    authors： Balbo BE,Amaral AG,Fonseca JM,de Castro I,Salemi VM,Souza LE,Dos Santos F,Irigoyen MC,Qian F,Chammas R,Onuchic LF

    更新日期：2016-09-01 00:00:00

  • Relationship of protein intake to urinary oxalate and glycolate excretion.

    abstract：:The relationship of protein intake to urinary oxalate and glycolate excretion was examined in a large cohort (N = 101) of normal individuals on self-selected diets and in 11 individuals on controlled protein diets. On self-selected diets no correlation was detected between protein intake and urinary oxalate or glycola...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1993.253

    authors： Holmes RP,Goodman HO,Hart LJ,Assimos DG

    更新日期：1993-08-01 00:00:00

  • Polycystic kidney and liver disease in Springbok: I. Morphology of the lesions.

    abstract：:Spontaneous polycystic kidney and liver disease in an inbred herd of Springbok (Antidorcas marsupialis) was studied by light microscopy, transmission electron microscopy, and microdissection. Springbok are a small species of gazelle of the family Bovidae native to South Africa. Since 1976, 18% of all live calves born ...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1982.146

    authors： Iverson WO,Fetterman GH,Jacobson ER,Olsen JH,Senior DF,Schobert EE

    更新日期：1982-08-01 00:00:00

  • Morphometric study of glomerular slit diaphragms fixed by rapid-freezing and freeze-substitution.

    abstract：:The widths of slit diaphragms in rat glomeruli were quantitatively measured on electron micrographs obtained by the rapid-freezing and freeze-substitution (FS) fixation method and compared to those prepared by conventional chemical fixation procedures, such as tannic acid, glutaraldehyde and osmium tetroxide (TGO) or ...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1991.253

    authors： Furukawa T,Ohno S,Oguchi H,Hora K,Tokunaga S,Furuta S

    更新日期：1991-10-01 00:00:00

  • Elimination of Candida albicans from kidneys of mice during short-term systemic infections.

    abstract：:The candidacidal activity of kidneys, liver, and spleen's phagocytic systems was studied in mouse. Different strains of mice were inoculated intravenously (i.v.) with 1 to 2.6 x 10(4) viable Candida albicans. Elimination of the microorganisms from the kidneys, liver, and spleen were evaluated by enumeration of colony ...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1991.225

    authors： Baghian A,Lee KW

    更新日期：1991-09-01 00:00:00

  • Immune evasion in renal cell carcinoma: biology, clinical translation, future directions.

    abstract：:Targeted therapies and immune checkpoint inhibitors have advanced the treatment landscape of Renal Cell Carcinoma (RCC) over the last decade. While checkpoint inhibitors have demonstrated survival benefit and are currently approved in the front-line and second-line settings, primary and secondary resistance is common....

    journal_title：Kidney international

    pub_type： 杂志文章,评审

    doi：10.1016/j.kint.2020.08.028

    authors： Wang X,Lopez R,Luchtel RA,Hafizi S,Gartrell B,Shenoy N

    更新日期：2021-01-01 00:00:00

  • Dual RAAS blockade is desirable in kidney disease: con.

    abstract：:Dual renin-angiotensin aldosterone (RAAS) blockade is associated with higher risk of hyperkalemia and has not been shown, in any outcome trial of validated renal end points, that is, doubling of creatinine, time to dialysis, or death, to be superior over other approaches. It shows promise in advanced proteinuric nephr...

    journal_title：Kidney international

    pub_type： 评论,杂志文章,评审

    doi：10.1038/ki.2010.226

    authors： Bakris GL

    更新日期：2010-09-01 00:00:00

  • Amiloride blocks lithium entry through the sodium channel thereby attenuating the resultant nephrogenic diabetes insipidus.

    abstract：:Lithium therapy frequently induces nephrogenic diabetes insipidus; amiloride appears to prevent its occurrence in some clinical cases. Amiloride blocks the epithelial sodium channel (ENaC) located in the apical membrane of principal cells; hence one possibility is that ENaC is the main entry site for lithium and the b...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.2009.91

    authors： Kortenoeven ML,Li Y,Shaw S,Gaeggeler HP,Rossier BC,Wetzels JF,Deen PM

    更新日期：2009-07-01 00:00:00

  • Predominant synthesis of IgA with lambda light chain in IgA nephropathy.

    abstract：:The nature of the light chains in mesangial IgA deposits and serum IgA was studied in patients with IgA nephropathy. Immunofluorescence (IF) studies using murine monoclonal antibodies, rabbit and goat anti-human monospecific antisera were performed in kidney sections from 15 IgA nephritic patients with only IgA isotyp...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1988.37

    authors： Lai KN,Chui SH,Lai FM,Lam CW

    更新日期：1988-02-01 00:00:00

  • Metabolomic study of cisplatin-induced nephrotoxicity.

    abstract：:We have shown that cisplatin inhibits fatty acid oxidation, and that fibrate treatment ameliorates renal function by preventing the inhibition of fatty acid oxidation and proximal tubule cell death. Urine samples of mice treated with single injection of cisplatin (20 mg/kg body weight) were collected for 3 days and an...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/sj.ki.5000433

    authors： Portilla D,Li S,Nagothu KK,Megyesi J,Kaissling B,Schnackenberg L,Safirstein RL,Beger RD

    更新日期：2006-06-01 00:00:00

  • Influence of hematocrit on the measurement of lipoproteins demonstrated by the example of lipoprotein(a).

    abstract：BACKGROUND:The measurement of many parameters of human blood is usually performed in plasma or serum. Since lipoproteins or apolipoproteins, for example, are found almost exclusively in the plasma fraction after low-speed centrifugation, these parameters can be expected to be distributed in a different plasma volume de...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1046/j.1523-1755.1998.00086.x

    authors： Kronenberg F,Trenkwalder E,Kronenberg MF,König P,Utermann G,Dieplinger H

    更新日期：1998-10-01 00:00:00

  • Humoral control of water and electrolyte excretion during water restriction.

    abstract：:The goals of the present study were twofold: first, to assess the renal excretory and hormonal responses to chronic water restriction in dogs whose sodium retaining mechanisms had been stimulated through dietary sodium (Na+) deprivation; second, to determine the mediator(s) of the natriuresis which was observed with w...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1986.121

    authors： Merrill DC,Skelton MM,Cowley AW Jr

    更新日期：1986-06-01 00:00:00

  • Binding of factor H to tubular epithelial cells limits interstitial complement activation in ischemic injury.

    abstract：:Factor H is a regulator of the alternative pathway of complement, and genetic studies have shown that patients with mutations in factor H are at increased risk for several types of renal disease. Pathogenic activation of the alternative pathway in acquired diseases, such as ischemic acute kidney injury, suggests that ...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.2011.115

    authors： Renner B,Ferreira VP,Cortes C,Goldberg R,Ljubanovic D,Pangburn MK,Pickering MC,Tomlinson S,Holland-Neidermyer A,Strassheim D,Holers VM,Thurman JM

    更新日期：2011-07-01 00:00:00

  • Nrf2 suppresses lupus nephritis through inhibition of oxidative injury and the NF-κB-mediated inflammatory response.

    abstract：:The generation of reactive oxygen species has a pivotal role in both acute and chronic glomerular injuries in patients with lupus nephritis. As the transcription factor Nrf2 is a major regulator of the antioxidant response and is a primary cellular defense mechanism, we sought to determine a role of Nrf2 in the progre...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.2013.343

    authors： Jiang T,Tian F,Zheng H,Whitman SA,Lin Y,Zhang Z,Zhang N,Zhang DD

    更新日期：2014-02-01 00:00:00

  • Cysteamine therapy delays the progression of nephropathic cystinosis in late adolescents and adults.

    abstract：:Nephropathic cystinosis is a multisystem autosomal recessive disease caused by cystine accumulation, which is usually treated by oral cysteamine. In order to determine long-term effects of this therapy, we enrolled 86 adult patients (mean age 26.7 years) diagnosed with nephropathic cystinosis, 75 of whom received cyst...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.2011.277

    authors： Brodin-Sartorius A,Tête MJ,Niaudet P,Antignac C,Guest G,Ottolenghi C,Charbit M,Moyse D,Legendre C,Lesavre P,Cochat P,Servais A

    更新日期：2012-01-01 00:00:00

  • A missense mutation in podocin leads to early and severe renal disease in mice.

    abstract：:Mutations in the NPHS2 gene, encoding podocin, are responsible for familial autosomal recessive and sporadic cases of steroid-resistant nephrotic syndrome. We have successfully generated a mouse model in which the common p.R138Q mutation found in nephrotic patients is expressed in the kidney. Homozygous mice express t...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.2008.27

    authors： Philippe A,Weber S,Esquivel EL,Houbron C,Hamard G,Ratelade J,Kriz W,Schaefer F,Gubler MC,Antignac C

    更新日期：2008-05-01 00:00:00

  • Beneficial impact of spironolactone in diabetic nephropathy.

    abstract：BACKGROUND:Aldosterone has been suggested to play a role in the initiation and progression of diabetic nephropathy. Currently recommended treatment with angiotensin-converting enzyme (ACE) inhibitors or angiotensin II receptor blockers [renin-angiotensin system (RAS) blockade] does not suppress circulating aldosterone ...

    journal_title：Kidney international

    pub_type： 杂志文章,随机对照试验

    doi：10.1111/j.1523-1755.2005.00756.x

    authors： Schjoedt KJ,Rossing K,Juhl TR,Boomsma F,Rossing P,Tarnow L,Parving HH

    更新日期：2005-12-01 00:00:00

  • The STARMEN trial indicates that alternating treatment with corticosteroids and cyclophosphamide is superior to sequential treatment with tacrolimus and rituximab in primary membranous nephropathy.

    abstract：:A cyclical corticosteroid-cyclophosphamide regimen is recommended for patients with primary membranous nephropathy at high risk of progression. We hypothesized that sequential therapy with tacrolimus and rituximab is superior to cyclical alternating treatment with corticosteroids and cyclophosphamide in inducing persi...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1016/j.kint.2020.10.014

    authors： Fernández-Juárez G,Rojas-Rivera J,Logt AV,Justino J,Sevillano A,Caravaca-Fontán F,Ávila A,Rabasco C,Cabello V,Varela A,Díez M,Martín-Reyes G,Diezhandino MG,Quintana LF,Agraz I,Gómez-Martino JR,Cao M,Rodríguez-Moreno A,

    更新日期：2020-11-07 00:00:00

  • Effect of vitamin D metabolites on calcitriol metabolism in experimental renal failure.

    abstract：:Previous studies from our laboratory have demonstrated that metabolic clearance rate (MCR) of calcitriol is decreased in experimental renal failure. In this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin D3 (25(OH)D3) and 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) on the MCR of calcitriol in renal ...

    journal_title：Kidney international

    pub_type： 杂志文章

    doi：10.1038/ki.1989.185

    authors： Patel S,Simpson RU,Hsu CH

    更新日期：1989-08-01 00:00:00