Abstract：
:The effect of activated protein C (APC) on agonist-induced platelet activation and on thrombin generation after intrinsic (IA) and extrinsic (EA) activation of the coagulation system was studied by flow cytometry and by measuring levels of prothrombin fragment F1+2. In platelet activation studies blood drawn from healthy volunteers was anticoagulated with 10 micrograms/ml APC and incubated at 37 degrees C either with saline, recombinant tissue factor (r-TF), arachidonic acid (AA), ADP or collagen. At definite times aliquots were taken and processed for flow studies. Platelet activation was measured using fluorescent monoclonal antibodies to platelet surface receptors GPIIIa (CD-61) and P-selectin (CD-62). Flow cytometric analysis showed platelet activation after all agonists used. APC did not influence AA-, ADP- and collagen-induced platelet activation but completely inhibited activation of platelets induced by r-TF. The effect of APC on r-TF-mediated platelet activation was concentration-dependent in the range of 0.5 to 20 micrograms/ml showing an increase in CD-62 expression at lower concentrations. In citrated and APC-anticoagulated blood the generation of thrombin was studied after IA and EA. At 10 and 20 micrograms/ml APC effectively prevented blood clotting which rapidly occurred especially after EA. The amount of thrombin generated via the extrinsic pathway was reduced by APC whereas after IA F1+2 levels measured in the presence of APC were still strongly increased. These results indicate that small amounts of thrombin generated by r-TF are sufficient to activate platelets as well as blood coagulation. APC exerts strong concentration-dependent anticoagulant actions and effectively prevents activation of platelets.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Kaiser B,Jeske W,Hoppensteadt DH,Walenga JM,Drohan W,Fareed Jdoi
10.1016/s0049-3848(97)00119-9subject
Has Abstractpub_date
1997-07-15 00:00:00pages
197-204issue
2eissn
0049-3848issn
1879-2472pii
S0049-3848(97)00119-9journal_volume
87pub_type
杂志文章abstract：INTRODUCTION:Excessive platelet activation fundamentally contributes to cardiovascular events and mortality in patients with diabetes mellitus. Functional resistance has been described for current antiplatelet therapies in broad populations that include patients with diabetes. We investigated acute and chronic effects ...
journal_title：Thrombosis research
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journal_title：Thrombosis research
pub_type： 杂志文章,meta分析
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更新日期：2010-06-01 00:00:00
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更新日期：1992-08-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.01.006
更新日期：2007-01-01 00:00:00
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更新日期：2003-03-15 00:00:00
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更新日期：1985-11-15 00:00:00
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更新日期：2009-07-01 00:00:00
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更新日期：2018-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1990-01-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
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更新日期：1982-12-15 00:00:00
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更新日期：2014-08-01 00:00:00
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pub_type： 临床试验,信件
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更新日期：2019-04-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1986-08-15 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.01.002
更新日期：2013-03-01 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90345-8
更新日期：1987-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0049-3848(90)90118-v
更新日期：1990-09-15 00:00:00
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journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.04.019
更新日期：2014-07-01 00:00:00
abstract：:Concentration-response relationships of human platelet aggregation rates were analyzed for a variety of agonists and inhibitors. Their approximation by the Hill equation showed that the values of the Hill coefficient (h) were agonist-dependent and increased as follows: hADP = hL-EPINEPHRINE = hPAF = hPGH2 = hU46619 le...
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pub_type： 杂志文章
doi：10.1016/0049-3848(92)90308-w
更新日期：1992-06-01 00:00:00
