Abstract：
:Effects of mechanical trauma of platelets and red blood cells on dynamic viscoelasticity of blood during clotting were examined. Two different methods were attempted to give the mechanical damage to blood cells. In one method, a flow apparatus consisting of a thin teflon tube and two sample reservoirs connecting to both ends of the tube was employed. After a blood sample repeated the shuttle flow many times through the tube, the dynamic rigidity modulus (G') and loss modulus (G") of the blood sample during clotting were measured. The mechanical trauma of platelets brought about the increase of G' and G" for clots of PRP and whole blood, in addition to shorter clotting time. Slight hemolysis of red blood cells (less than 0.5% of total hemolysis) only yielded shorter clotting time for whole blood. In other method, packed red blood cells were hemolyzed by exposing the ultrasonic wave (the amount of hemolysis was about 5% of total hemolysis). Addition of supernatant of hemolyzed red blood cells to PRP caused the platelet aggregation. Furthermore, a marked hemolysis brought about higher values of G' and G" for blood clot, in addition to shorter clotting time. These results suggest that the release of constituents from damaged red blood cells and alteration of cell surface due to mechanical damage would accelerate the coagulation sequence.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Kaibara M,Ukiya Y,Sekiguchi Mdoi
10.1016/0049-3848(86)90084-8subject
Has Abstractpub_date
1986-08-15 00:00:00pages
395-408issue
4eissn
0049-3848issn
1879-2472journal_volume
43pub_type
杂志文章abstract：INTRODUCTION:Plasma viscosity (PV) constitutes an independent important predictor of initial and recurrent cardiac events and mortality. It has been suggested that there is a geographical variation in PV values related to coronary event rates. Little information exists regarding PV in Spain. Therefore, our objective wa...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2006.11.009
更新日期：2007-01-01 00:00:00
abstract：:The pharmacodynamic and pharmacokinetic properties of endpoint-attached N'alkylamine derivatives of low molecular mass heparin (LMMH), low molecular mass heparin (LMMH), low molecular mass heparin-tyramine (LMMH-tyr) and low molecular mass heparin-tyramine-fluorescein-5-isothiocyanate (LMMH-tyr-fitc) were investigated...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00172-n
更新日期：1995-11-01 00:00:00
abstract：:The use of platinum based chemotherapy in ovarian malignancy and other cancer types is known to be associated with deep vein thrombosis. In a prospective study of 47 patients with ovarian cancer of International Federation of Gynecology and Obstetrics stage Ib-IV, serial rheological parameters were determined (plasma ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00022-x
更新日期：1998-04-15 00:00:00
abstract：BACKGROUND/AIM:It is believed that the amniotic fluid-derived TF, in the case of amniotic fluid embolism (AFE), contributes to acute disseminated intravascular coagulation (DIC) and obstetric shock in the mother. However, the role of amniotic fluid-derived contact phase coagulation factors that irrupt into the bloodstr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.10.014
更新日期：2015-04-01 00:00:00
abstract：:Venous thromboembolism (VTE) is a common complication in patients with malignant disease. In addition to well-established acquired risk factors for VTE, several genetic risk factors, mainly related to the haemostatic system, are known to influence thrombotic risk. However, the contribution of gene abnormalities to thr...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(03)00179-8
更新日期：2003-02-15 00:00:00
abstract：:Two functional assays for protein C inhibitor (PCI) were evaluated in parallel for the same plasma samples. One assay is specific for active PCI and measures its ability to form complexes with activated protein C (APC) in the presence of heparin (España et al, Thromb Res 64, 309, 1991) (Method A). After incubation of ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(93)90079-4
更新日期：1993-06-01 00:00:00
abstract：:Plasma from patients with thrombotic thrombocytopenic purpura (TTP) caused the aggregation of washed human platelets, which was inhibited by preincubation with normal plasma. Using salt fractionation, ion exchange chromatography, and preparative agarose gel electrophoresis, we purified a protein from normal plasma whi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(84)90155-5
更新日期：1984-01-01 00:00:00
abstract：:Selected coagulation and fibrinolytic parameters were assessed in 40 insulin dependent diabetes mellitus patients with varying degrees of metabolic control; 30 healthy subjects matched for age and sex formed the control group. Activated Partial Thromboplastin Time, Prothrombin Time, Fibrinogen, Factor VII, Antithrombi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(92)90068-l
更新日期：1992-09-15 00:00:00
abstract：:A Ca++-ionophore, ionomycin, increased the volume of human platelets suspended in a Ca++-containing buffer. This change in cell volume was dependent upon ionomycin and extracellular Ca++ concentrations, suggesting that the volume change occurs when the intracellular Ca++ reaches a certain level (greater than uM as det...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90197-k
更新日期：1990-01-01 00:00:00
abstract：:The effects of platelets on clot lysis and plasminogen activation by staphylokinase (SAK) were investigated. At concentrations ranging from 2 x 10(-7) to 1 x 10(-5)g/ml of SAK, the lysis time of platelet-rich plasma clots (PRP-clots) was shorter than that of platelet-poor plasma clots (PPP-clots). This reduction of cl...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)00159-o
更新日期：1995-10-15 00:00:00
abstract：:The effect of extracellular matrix components on lipopolysaccharide-induced vascular endothelial cell injury was studied by using lipopolysaccharide-susceptible bovine aortic endothelial cells. For evaluation of lipopolysaccharide-induced injury, we estimated DNA synthesis and cell detachment of bovine aortic endothel...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(99)00226-1
更新日期：2000-04-15 00:00:00
abstract：BACKGROUND:Intravenous fluids can impair coagulation and affect the endothelial glycocalyx, whereas glycocalyx shedding itself can cause an impairment of clot formation and firmness. We hypothesized that hydroxyethyl starch 6% (130/0.4) has a more distinct effect on coagulation and glycocalyx shedding than albumin 5%. ...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2019.10.020
更新日期：2019-11-01 00:00:00
abstract：INTRODUCTION:The effects of multiple inherited and acquired thrombophilic defects on the outcome of in-vitro fertilization (IVF) remain unexplored. The aim of this study was to evaluate the association between multiple thrombophilia and clinical outcomes in a large prospective cohort of women undergoing IVF. MATERIALS...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.05.006
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:In the rat liver, growth hormone (GH) affects the synthesis of vitamin-K-dependent factors, including Protein C (prot.C) and protein S (prot.S), two natural anticoagulants that prevent hypercoagulable states. Adults with GH deficiency (GHD) are at risk of thrombotic events. High circulating levels of PAI-1 a...
journal_title：Thrombosis research
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.thromres.2010.08.028
更新日期：2010-12-01 00:00:00
abstract：:Addition of two commercial luciferin-luciferase reagents caused marked inhibition of the aggregatory response of washed human platelets to thrombin, ADP, vasopressin and platelet-activating factor (PAF). Analysis of the effects of the individual components of one of these reagents revealed that Mg2+, and to a lesser e...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(85)90053-2
更新日期：1985-04-15 00:00:00
abstract：INTRODUCTION:Reports on the prevalence and risk factors of atherothrombotic events (AEs) are conflicting in persons with hemophilia (PWH). METHODS:This study evaluated the prevalence and risk factors of AEs among 1054 male hemophilia patients, using data collected from Taiwan's National Health Insurance Research Datab...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.12.027
更新日期：2015-03-01 00:00:00
abstract：INTRODUCTION:Growth differentiation factor-15 (GDF-15) has been identified as a predictor in cardiovascular diseases and acute pulmonary embolism. However, the association of GDF-15 and deep venous thrombosis (DVT) remains unclear. This study aimed to investigate levels of GDF-15 in patients with DVT, and determine its...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2020.01.022
更新日期：2020-03-01 00:00:00
abstract：:Analogs of the antithrombin peptide hirudin54-65 with C-terminal modifications have been synthesized in order to examine the requirements for alpha-thrombin inhibition. The C-terminal residue, Gln65, could be replaced with L-amino acids or amino alcohols with neutral or charged hydrophilic side chains without greatly ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90090-x
更新日期：1989-05-15 00:00:00
abstract：:Optimal conditions for a method to simultaneously measure aggregation in 96 samples using a microplate reader were developed. The temperature of the assay was set at 25 degrees C, the optimal platelet concentration range was determined to be from 1-3 x 10(8) per mL, the assay volume was determined to be best at 100 mi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(95)93881-y
更新日期：1995-03-01 00:00:00
abstract：:The efficacy and the safety of a new urokinase dosage regimen (loading dose 250,000 IU, initial maintenance dosage 2,000 IU/kg/h in combination with heparin) was studied in ten cases of deep vein thrombosis with regard to the changes of the blood coagulation and fibrinolytic enzyme system. The coagulation analyses dem...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(82)90210-9
更新日期：1982-01-01 00:00:00
abstract：:Although effective therapies for haemophilia have been available for decades, the prevention and treatment of joint disease remain major clinical concerns for all haemophilia patients. Early identification of joint disease is vital to initiate or modify treatment, and prevent arthropathy. However, there remains a need...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2014.02.015
更新日期：2014-06-01 00:00:00
abstract：:Ondansetron is a 5-HT3 receptor antagonist with potent antiemetic, analgesic, and antiphlogistic effects. Literature concerning 5-HT3 antagonists on platelets is limited. In this report we examined the pharmacological effects of ondansetron on human washed platelets. Platelet aggregation induced by thrombin (0.1 U/mL)...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2012.06.003
更新日期：2012-09-01 00:00:00
abstract：:The clinical feature in patients with congenital factor VII deficiency is in part dependent on the underlying genetic defect, but the mechanisms influencing the genotype-phenotype correlation remain to be fully elucidated. In addition, thromboembolic events have been reported. Compensatory mechanisms involving vitamin...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00410-2
更新日期：2001-03-15 00:00:00
abstract：INTRODUCTION:There is some controversy regarding the effect of CYP2C19 polymorphism on clinical outcome in patients with dual antiplatelet therapy. Chronic kidney disease (CKD) is associated with increased risk of cardiovascular event, but the association between the possession of CYP2C19 loss-of-function (LOF) alleles...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2014.07.039
更新日期：2014-11-01 00:00:00
abstract：INTRODUCTION:In the prospective, randomised, double-blind, placebo-controlled Regenerate Vital Myocardium by Vigorous Activation of Bone Marrow Stem Cells (REVIVAL)-2 trial patients with acute myocardial infarction (AMI) and successful mechanical reperfusion received granulocyte-colony stimulating factor (G-CSF, 10 μg/...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2010.11.004
更新日期：2011-02-01 00:00:00
abstract：:D-dimer of human fibrin was used as antigen to obtain monoclonal antibodies (mAbs). We have obtained 16 hybridomas producing mAbs of different specificity. Only two of these mAbs inhibited fibrin polymerization. They are of the IgG-class. One mAb (II-4d) inhibited fibrin polymerization to 100% and another (II-3b) to 6...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2004.03.005
更新日期：2004-01-01 00:00:00
abstract：INTRODUCTION:Recombinant members of the vitamin K-dependent protein family (factors IX and VII and protein C) have become important pharmaceuticals in treatment of bleeding disorders and sepsis. However, because the in vivo gamma-carboxylation system in stable cell lines used for transfection has a limited capacity of ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.11.002
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:The methodology of thromboprophylaxis post minimally invasive esophagectomy (MIE) is unclear. Thus, we compared the efficacy and safety of fondaparinux and nadroparin on the prophylaxis of venous thromboembolism (VTE) after MIE. MATERIALS AND METHODS:We conducted a randomized, double-blind, treatment-contro...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2018.04.001
更新日期：2018-06-01 00:00:00
abstract：:Platelets occupy a central role at the interface between thrombosis and inflammation. At sites of vascular damage, adherent platelets physically and functionally interact with circulating leukocytes. Activated platelets release soluble factors into circulation that may have local and systemic effects on blood and vasc...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2010.10.010
更新日期：2011-03-01 00:00:00
abstract：:Thrombin was acetylated by treatment with acetic anhydride, and the potency for protein C activation and the fibrinogen clotting activity of the resultant acetylthrombin were investigated in vitro and in vivo. The acetylthrombin retained an amidolytic activity to the synthetic substrate, S-2238, and reduced both of th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90053-f
更新日期：1990-08-15 00:00:00
