Abstract：
:The immunoreceptors NKG2D and NKp46 are known for their capacity to activate natural killer (NK) cell cytotoxicity and secretory responses in the contexts of tumors and infections, yet their roles in NK cell education remain unclear. Here, we provide the first characterization of mice deficient for both NKG2D and NKp46 receptors to address the relevance of their concomitant absence during NK cell development and function. Our findings reveal that NK cells develop normally in double-mutant (DKO) mice. Mice lacking NKG2D but not NKp46 showed subtle differences in the percentages of NK cells expressing inhibitory Ly49 receptors and the adhesion molecule DNAM-1. A slightly increased percentage of terminally differentiated NK cells and functional response to in vitro stimuli was observed in some experiments. These alterations were modest and did not affect NK cell function in vivo in response to mouse cytomegalovirus infection. NKp46 deficiency alone, or in combination with NKG2D deficiency, had no effect on frequency or function of NK cells.
journal_name
Bloodjournal_title
Bloodauthors
Sheppard S,Triulzi C,Ardolino M,Serna D,Zhang L,Raulet DH,Guerra Ndoi
10.1182/blood-2012-12-471607subject
Has Abstractpub_date
2013-06-20 00:00:00pages
5025-33issue
25eissn
0006-4971issn
1528-0020pii
blood-2012-12-471607journal_volume
121pub_type
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