Abstract:
:We document findings on c-kit (CD117) expression in 1,937 pediatric and adult de novo acute leukemia cases, diagnosed in five single European centers. All cases were well characterized as to the morphologic, cytochemical, and immunologic features, according to the European Group for the Immunological Classification of Leukemias (EGIL). The cases included 1,103 acute myeloid leukemia (AML), 819 acute lymphoblastic leukemia (ALL), 11 biphenotypic acute leukemia (BAL), and 4 undifferentiated (AUL). c-kit was expressed in 741 (67%) AML cases, regardless of the French-American-British (FAB) subtype, one third of BAL, all four AUL, but only in 34 (4%) of ALL cases. The minority of c-kit+ ALL cases were classified as: T-cell lineage (two thirds), mainly pro-T-ALL or T-I, and B lineage (one third); cells from 62% of these ALL cases coexpressed other myeloid markers (CD13, CD33, or both). There were no differences in the frequency of c-kit+ AML or ALL cases according to age being similar in the adult and pediatric groups. Our findings demonstrate that c-kit is a reliable and specific marker to detect leukemia cells committed to the myeloid lineage, and therefore should be included in a routine basis for the diagnosis of acute leukemias to demonstrate myeloid commitment of the blasts. c-kit expression should score higher, at least one point, in the system currently applied to the diagnosis of BAL, as its myeloid specificity is greater than CD13 and CD33. Findings in ALL and AUL suggest that c-kit identifies a subgroup of cases, which may correspond to leukemias either arising from early prothymocytes and/or early hematopoietic cells, both able to differentiate to the lymphoid and myeloid pathways.
journal_name
Bloodjournal_title
Bloodauthors
Bene MC,Bernier M,Casasnovas RO,Castoldi G,Knapp W,Lanza F,Ludwig WD,Matutes E,Orfao A,Sperling C,van't Veer MBsubject
Has Abstractpub_date
1998-07-15 00:00:00pages
596-9issue
2eissn
0006-4971issn
1528-0020journal_volume
92pub_type
杂志文章相关文献
BLOOD文献大全abstract::Natural-killer (NK)-cell dysfunction and IFN-gamma deficiencies have been associated with increased incidence of both malignancy and infection. The immunologic basis of NK-cell defects in cancer-bearing hosts has not been extensively studied. Here, we demonstrate that multiple lineages of tumors, including thymoma, br...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-11-4535
更新日期:2006-07-01 00:00:00
abstract::Immune deficiency viruses such as SIV in macaques or HIV-1 in human beings have evolved mechanisms to defeat host immunity that also impact the efficacy of vaccines. A key factor for vaccine protection is whether immune responses elicited by prior immunization remain at levels sufficient to limit disease progression o...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-02-202655
更新日期:2009-08-06 00:00:00
abstract::To study the role of different cytokine combinations on the proliferation and differentiation of highly purified primitive progenitor cells, a serum-free liquid culture system was used in combination with phenotypic and functional analysis of the cells produced in culture. CD34+ CD45RAlo CD71lo cells, purified from um...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-06-15 00:00:00
abstract::We used both radiolabeled and fluorescein-labeled antiglobulins in assays to detect antibodies against platelets in multiply transfused patients to determine the value of these tests in predicting the outcome of platelet transfusion in such patients. In 15 allosensitized patients, we studied 68 single-donor platelet t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Recurrence of the underlying malignancy remains a major cause of treatment failure after autologous bone marrow transplantation (BMT) for patients with lymphoma. In this regard, we have developed an immunotherapeutic approach designed to induce resistance against residual tumor cells persisting after BMT. Previous stu...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract::Essential thrombocythemia (ET) and primary myelofibrosis (PMF) are chronic diseases characterized by clonal hematopoiesis and hyperproliferation of terminally differentiated myeloid cells. The disease is driven by somatic mutations in exon 9 of CALR or exon 10 of MPL or JAK2-V617F in >90% of the cases, whereas the rem...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-07-661835
更新日期:2016-01-21 00:00:00
abstract::To identify molecularly defined subgroups in multiple myeloma, gene expression profiling was performed on purified CD138(+) plasma cells of 320 newly diagnosed myeloma patients included in the Dutch-Belgian/German HOVON-65/GMMG-HD4 trial. Hierarchical clustering identified 10 subgroups; 6 corresponded to clusters desc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-12-261032
更新日期:2010-10-07 00:00:00
abstract::The Kell blood group is one of the major antigenic systems in human red blood cells. To determine the location of the Kell gene on human chromosomes, panels containing genomic DNA of human-hamster somatic cell hybrids were hybridized with radiolabeled cDNA probe specific for the Kell locus. Only the samples containing...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-05-15 00:00:00
abstract::During development, mice with mutations of stem cell factor (SCF) or its receptor c-kit exhibit defects in melanogenesis, as well as hematopoiesis and gonadogenesis. Because melanocytes derive from neural crest cells, the role of SCF and c-kit was investigated in the neural crest-derived childhood tumor neuroblastoma....
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-11-15 00:00:00
abstract::Conventional chemotherapy has failed to substantially prolong survival for patients with advanced follicular lymphoma. To improve outcomes, the German Low-Grade Lymphoma Study Group (GLSG) initiated a randomized trial to compare the effect of potentially curative myeloablative radiochemotherapy followed by autologous ...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2004-03-0982
更新日期:2004-11-01 00:00:00
abstract::Systemic inflammation perturbs the bone marrow environment by evicting resident B cells and favoring granulopoiesis over lymphopoiesis. Despite these conditions, a subset of marrow B cell remains to become activated and produce potent acute immunoglobulin M (IgM) responses. This discrepancy is currently unresolved and...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-03-635805
更新日期:2015-09-03 00:00:00
abstract::Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Platelet packaging and release concludes at the tips of each proplatelet. Essential in this process is the distribution of organelles and platelet-specific granules i...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-06-2206
更新日期:2005-12-15 00:00:00
abstract::Essential thrombocythemia (ET) and polycythemia vera (PV) are characterized by persistent platelet activation. The mechanisms involved in their clearance are poorly characterized. In the present study, we report that leukocytes were actively involved in platelet disposal in 51 patients with ET and 30 with PV, but not ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-02-337337
更新日期:2011-09-22 00:00:00
abstract::WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome is an immune deficiency linked in many cases to heterozygous mutations causing truncations in the cytoplasmic tail of CXC chemokine receptor 4 (CXCR4). Leukocytes expressing truncated CXCR4 display enhanced responses to the receptor ligand CXC...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-07-102103
更新日期:2008-07-01 00:00:00
abstract::X-linked severe combined immunodeficiency (XSCID) is characterized by absent or profoundly reduced numbers of T cells and normal numbers of B cells in the circulation. Affected patients have mutations of the interleukin-2 (IL-2) receptor gamma chain gene. Using Epstein-Barr virus-transformed B-lymphoblastoid cell line...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-08-15 00:00:00
abstract::Exposed subendothelial collagen acts as a substrate for platelet adhesion and thrombus formation after vascular injury. Synthetic collagen-derived triple-helical peptides, designated collagen-related peptide (CRP), GFOGER, and VWF-III, can specifically engage the platelet collagen receptors, glycoprotein VI and integr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-01-260778
更新日期:2010-06-17 00:00:00
abstract::In this issue of Blood, Mayer et al describe a new method of generating high numbers of Batf3- and Irf8-dependent CD103+ conventional dendritic cells (cDCs), providing new opportunities to study this subset of antigen-presenting cells specialized in crosspresentation. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2014-08-595298
更新日期:2014-11-13 00:00:00
abstract::Pain is the leading cause of emergency department visits, hospitalizations, and daily suffering in individuals with sickle cell disease (SCD). The pathologic mechanisms leading to the perception of pain during acute RBC sickling episodes and development of chronic pain remain poorly understood and ineffectively treate...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-12-327429
更新日期:2011-09-22 00:00:00
abstract::Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening hyperinflammatory disease. Inherited forms of HLH are caused by biallelic mutations in several effectors of granule-dependent lymphocyte-mediated cytotoxicity. A small proportion of patients with a so-called "secondary" form of HLH, which develops in the a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-12-688960
更新日期:2016-04-28 00:00:00
abstract::In primary human T cells, anergy induction results in enhanced p59Fyn activity. Because Fyn is the kinase primarily responsible for the phosphorylation of PAG (the phosphoprotein associated with glycosphingolipid-enriched microdomains), which negatively regulates Src-kinase activity by recruiting Csk (the C-terminal S...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-07-038752
更新日期:2007-07-15 00:00:00
abstract::Previously, it was believed that megakaryocytopoiesis was regulated by two types of humoral factors: megakaryocyte colony-stimulating factor (MK-CSF), which acts on progenitors inducing their proliferation, and thrombopoietin (TPO), a megakaryocyte(s) (MK) maturational factor that induces platelet formation. The recen...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::The expression and function of a glycoprotein Ib (GPIb) complex on human umbilical vein endothelial cells (HUVECs) is still a matter of controversy. We characterized HUVEC GPIb using viper venom proteins: alboaggregins A and B, echicetin, botrocetin, and echistatin. Echicetin is an antagonist, and alboaggregins act as...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-04-15 00:00:00
abstract::Interaction of platelets with collagen under conditions of blood flow is a multi-step process with tethering via glycoprotein IbIXV (GPIbIXV) over von Willebrand factor, adhesion by direct interaction with the integrin GPIaIIa, and signaling via GPVI. GPVI can be specifically agonized by cross-linked collagen-related ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-08-2591
更新日期:2003-06-01 00:00:00
abstract::Nitric oxide (NO) exerts vasodilatatory, antiplatelet, antioxidant, and antiproliferative effects. Endothelium-derived NO has been shown to be of crucial importance in cardiovascular protection, whereas evidence that NO is synthesized by platelets and regulates platelet function is still controversial. By using a sens...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-06-579474
更新日期:2015-01-22 00:00:00
abstract::The treatment landscape for multiple myeloma has been transformed by the introduction of novel agents, including immunomodulatory drugs, proteasome inhibitors, and monoclonal antibodies. These have been shown to be more effective and generally better tolerated than conventional chemotherapy, with their introduction in...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2017-05-693606
更新日期:2018-09-13 00:00:00
abstract::Dendritic cells (DCs) represent a small and heterogeneous fraction of the hematopoietic system, specialized in antigen capture, processing, and presentation. The different DC subsets act as sentinels throughout the body and perform a key role in the induction of immunogenic as well as tolerogenic immune responses. Bec...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2011-11-370130
更新日期:2012-04-12 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) is characterized by delayed senescence and slow accumulation of monoclonal, small lymphocytes. Basic fibroblast growth factor (bFGF) is a pleiotropic cytokine that plays a role in hematopoiesis and apoptosis. Elevated bFGF levels have been detected in urine from patients with a varie...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::In individuals with sickle cell disease, a variable number of irreversibly sickled cells (ISC) is present that may contribute to the pathophysiology of sickle cell anemia. The present study was undertaken to determine the possible role of membrane lipid peroxidation in the genesis of ISC. After 24 hr of simple aerobic...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract::A specially designed rotational viscometer was used to investigate the effects of the antiplatelet agent PGI2 in combination with theophylline on the response of human platelets subjected to shear stress. Samples of citrated platelet-rich plasma (PRP) were exposed to shear stress in the viscometer for a period of 5 mi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::The translocation t(8;14)(q24;q32) is the characteristic chromosomal aberration of Burkitt's-type lymphomas and leukemias (BLs). On the molecular level, the t(8;14) juxtaposes the c-myc gene in 8q24 next to the IgH locus in 14q32, resulting in overexpression of the transcription factor c-Myc. The detection of a t(8;14...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00