Abstract：
:A phospholipase A2 (OHV A-PLA2) from the venom of Ophiophagus hannah (King cobra) is an acidic protein exhibiting antiplatelet activity. In in vitro tests, OHV APLA2 showed a marked inhibitory effect on platelet aggregation induced by ADP, collagen and arachidonic acid in both human whole blood and platelet-rich plasma in a dose-dependent manner. The antiplatelet effects of OHV A-PLA2 did not increase when preincubation times of platelets and OHV A-PLA2 were prolonged indicating phospholipid hydrolysis did not significantly contribute to the antiplatelet effects. Alkylation of active site His residue using p-bromophenacyl bromide resulted in the complete loss of enzymatic activity, but the modified enzyme retained more than 30% of its antiplatelet effects. These results indicate that the antiplatelet effects of OHV A-PLA2 appear to be independent of its enzymatic activity, and there are separate sites responsible for the catalytic and antiplatelet activities.
journal_name
Life Scijournal_title
Life sciencesauthors
Huang MZ,Gopalakrishnakone P,Kini RMdoi
10.1016/s0024-3205(97)00923-5subject
Has Abstractpub_date
1997-01-01 00:00:00pages
2211-7issue
22eissn
0024-3205issn
1879-0631pii
S0024320597009235journal_volume
61pub_type
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