Abstract:
:Whole cell membrane currents induced by the inflammatory mediators, bradykinin, 5-hydroxytryptamine (5-HT) and prostaglandin E2, were investigated in capsaicin-sensitive dorsal root ganglion (DRG) neurons from newborn rats grown on a monolayer of hippocampal glia without nerve growth factor (NGF). When firmly attached to an underlying cell, the neurons survived >14 days without growing extensive processes. A majority of the small diameter neurons ( approximately 80%) exhibited sensitivity to capsaicin (3-6 muM) and this was enhanced in solution of low pH. In acidic extracellular solution (pH 6.1), the combination of bradykinin (10 microM), 5-HT (10 microM) and prostaglandin E2 (1 microM) induced an inward membrane current in all capsaicin-sensitive DRG neurons (n = 43). The current exceeded the sustained, low pH-induced membrane current by 205 +/- 53 (SE) pA. The combination of acidic inflammatory mediators was ineffective in cells that were insensitive to capsaicin. In capsaicin-sensitive neurons, the inflammatory mediators when applied singly or in any combination of two, induced no membrane currents or small current at pH 7.3 and 6.1. Capsazepine (10 microM), the capsaicin antagonist, completely inhibited the facilitatory action of inflammatory mediator combination but not the sustained inward current induced by acidic extracellular solution (pH 6.1 or 5.5). It is suggested that the inflammatory mediators, bradykinin,5-HT, and prostaglandin E2 together act as endogenous mediators at capsaicin receptors to generate an inward current when the ion channel is protonized.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Vyklický L,Knotková-Urbancová H,Vitásková Z,Vlachová V,Kress M,Reeh PWdoi
10.1152/jn.1998.79.2.670subject
Has Abstractpub_date
1998-02-01 00:00:00pages
670-6issue
2eissn
0022-3077issn
1522-1598journal_volume
79pub_type
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